RityA.18398.a

VirD4 protein of the type IV secretion system

CENTER ID: RityA.18398.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
RityA.18398.a.B2.GE35077 virD4 domain 98 591
RityA.18398.a.B21.GE38034 virD4 domain 98 591
RityA.18398.a.B22.GE41487 virD4 domain 98 591
RityA.18398.a.B42.GE41488 GenScript codon-optimized truncation ("delta104") 104 591
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_145058
PATRIC ID: fig|257363.4.peg.292
RefSeq: YP_067246.1
UniProt: Q68X78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEWHKILKVT RNIFGHAIIH PVVIFCTIWI SGVFIAIFTN EIGALGADIN AINITYKWAY WLINVWRQLK IADYNYLKLK LIISLLGPAI IVIIFYIKNF QRIKSLQFFE QPEKVYGDAS WANQSDIEAA GLRSKKGMLI GVDAGGYFVA DGFQHALLFA PTGSGKGVGF VIPNLLFWTD SVVVHDIKLE NHNLTSGWRE KQGQKVFVWE PSNPDGITHC YNPIDWVSTK PGQMVDDVQK ISNLIMPEKD FWNNEARSLF LGVTLYLIAD PTKTKSFGEV VRTMRSDDVV YNLAVVLDTL GGVIHPVAYM NIAAFLQKAD KERSGVISTM NSSLELWANP LIDSATASSD FNIQEFKKVK TTVYVGLTPD NIQRLQKLMQ VFYQQATEFL SRKMPDLKEE PYGVMFLLDE FPTLGKMDTF KAGIAYFRGY RVRLFLIIQD TQQLKGTYED AGMNSFLSNS TYRITFAANN YETANLISQL VGNKTVEQRS FSKPLFFDLN ISTRTQNVSQ VQRALLLPQE VIQLPRDEQI VLIESFPPIK SRKIKYYEDK FFTSRLLPPT FVPTQVPFNH VSNNSDASKE TETTTAIENN E
NT Sequence
atggaatggc ataagatact taaagttact aggaatatat tcggtcatgc tataattcat ccggttgtta ttttttgcac catttggatc agtggtgtgt ttattgcaat ttttactaat gagatcggag ctttaggtgc agacatcaat gctataaata ttacttataa gtgggcttac tggcttatta atgtttggcg gcaattaaaa attgccgatt ataattattt gaaattgaag ctaattatat ctcttcttgg tcctgctatt attgttataa tattttatat taaaaatttt caaagaataa aatcattaca attttttgag cagcctgaaa aagtatatgg tgacgctagt tgggctaatc agtctgatat agaagctgct ggtcttagat caaaaaaagg catgttaata ggtgttgatg ctggtggata ttttgttgca gatgggtttc agcatgcttt attattcgca cctaccggtt caggtaaagg tgttggtttt gtaataccta accttttatt ttggactgat tcagtagtag tacatgatat aaagcttgaa aatcacaatt tgactagtgg ttggcgtgag aaacaagggc aaaaagtttt tgtttgggaa ccttctaatc cagatggtat tacacattgt tataatccga ttgattgggt tagtactaag cctgggcaaa tggttgatga tgtacagaaa atttcgaatc ttataatgcc agaaaaggat ttttggaaca atgaagcacg tagtttattc ttaggcgtaa ctttatattt aatagctgat cctactaaaa ctaaatcttt cggtgaagta gtacgtacaa tgagaagtga cgatgtagtt tataatctag ccgttgtact tgatacgctt ggtggtgtaa tacatcctgt tgcatatatg aatattgctg catttttgca aaaagctgat aaagagcgtt ccggtgtaat atctacaatg aattcatcac ttgaattatg ggcaaatcca cttattgatt ctgctactgc atcttctgac ttcaatatac aagaatttaa aaaagttaaa acaacagtgt atgtaggatt aacacctgat aatatacagc gtttgcaaaa attaatgcag gtattttatc agcaggctac agaattttta agccgtaaaa tgcctgattt aaaggaggaa ccatatggag taatgttctt acttgatgag ttcccgactc ttggaaagat ggatactttt aaagctggta tcgcttattt tagaggttat agagttcggt tatttttgat tatccaagat acgcagcaat taaaaggaac atatgaggac gccggtatga attctttctt atctaattcc acatatcgca ttacctttgc tgctaataac tatgaaactg caaatttaat atcgcagctt gttggtaata agactgtaga acaaaggtca tttagtaaac ctttattttt tgaccttaat atttctacta gaacccaaaa tgtatctcaa gttcaaagag ctttactttt acctcaagaa gtaatacagt taccgcgaga tgagcaaatt gttttaattg aatccttccc gcctataaaa tctcgtaaaa ttaagtatta cgaagataag ttttttacta gtagattatt accgccgact tttgtaccta ctcaagtacc ctttaatcat gtgtcaaata atagtgatgc ttctaaagag actgaaacaa caactgctat cgaaaataat gagtaa
Details for RityA.18398.a.B2.GE35077
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/16/2012
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 75
Validated AA Sequence
MAHHHHHHMK NFQRIKSLQF FEQPEKVYGD ASWANQSDIE AAGLRSKKGM LIGVDAGGYF VADGFQHALL FAPTGSGKGV GFVIPNLLFW TDSVVVHDIK LENHNLTSGW REKQGQKVFV WEPSNPDGIT HCYNPIDWVS TKPGQMVDDV QKISNLIMPE KDFWNNEARS LFLGVTLYLI ADPTKTKSFG EVVRTMRSDD VVYNLAVVLD TLGGVIHPVA YMNIAAFLQK ADKERSGVIS TMNSSLELWA NPLIDSATAS SDFNIQEFKK VKTTVYVGXT PDNIQRLXKL MXXFYQQATE FLSRKMPDLK EEXYGVMXLL DEFPTXERXI LLKLVSLIXX XXVRXXLXXX XXAXXXMXXX XXXXXXXXSX XLLLXXXXXX XX
Validated NT Sequence
ttttgnnnnn nttaagaagg aganatacca tggctcacca ccaccaccac catatgaaaa attttcaaag aataaaatca ttacaatttt ttgagcagcc tgaaaaagta tatggtgacg ctagttgggc taatcagtct gatatagaag ctgctggtct tagatcaaaa aaaggcatgt taataggtgt tgatgctggt ggatattttg ttgcagatgg gtttcagcat gctttattat tcgcacctac cggttcaggt aaaggtgttg gttttgtaat acctaacctt ttattttgga ctgattcagt agtagtacat gatataaagc ttgaaaatca caatttgact agtggttggc gtgagaaaca agggcaaaaa gtttttgttt gggaaccttc taatccagat ggtattacac attgttataa tccgattgat tgggttagta ctaagcctgg gcaaatggtt gatgatgtac agaaaatttc gaatcttata atgccagaaa aggatttttg gaacaatgaa gcacgtagtt tattcttagg cgtaacttta tatttaatag ctgatcctac taaaactaaa tctttcggtg aagtagtacg tacaatgaga agtgacgatg tagtttataa tctagccgtt gtacttgata cgcttggtgg tgtaatacat cctgttgcat atatgaatat tgctgcattt ttgcaaaaag ctgataaaga gcgttccggt gtaatatcta caatgaattc atcacttgaa ttatgggcaa atccacttat tgattctgct actgcatctt ctgacttcaa tatacaagaa tttaaaaaag ttaaaacaac agtgtatgta ggantaacac ctgataatat acagcgtttg cnaaaattaa tgcangnatt ttatcagcag gctacagaat ttttaagccg taaaatgcct gatttaaagg aggaancata tggagtaatg ntcttacttg atgagttccc nactntngaa aganggatac ttttaaagct ggtatcgctt atttanannt nnngagttcg ntnnnttttg atnnnncant nnncagcnnn annnnntatg agnngnnnnn tgannnnnnn tannnntcnn nnatcnnntn cnttgctgct annnnnnnnn aannncannt nannnct
Expected Protein Sequence
MAHHHHHHMK NFQRIKSLQF FEQPEKVYGD ASWANQSDIE AAGLRSKKGM LIGVDAGGYF VADGFQHALL FAPTGSGKGV GFVIPNLLFW TDSVVVHDIK LENHNLTSGW REKQGQKVFV WEPSNPDGIT HCYNPIDWVS TKPGQMVDDV QKISNLIMPE KDFWNNEARS LFLGVTLYLI ADPTKTKSFG EVVRTMRSDD VVYNLAVVLD TLGGVIHPVA YMNIAAFLQK ADKERSGVIS TMNSSLELWA NPLIDSATAS SDFNIQEFKK VKTTVYVGLT PDNIQRLQKL MQVFYQQATE FLSRKMPDLK EEPYGVMFLL DEFPTLGKMD TFKAGIAYFR GYRVRLFLII QDTQQLKGTY EDAGMNSFLS NSTYRITFAA NNYETANLIS QLVGNKTVEQ RSFSKPLFFD LNISTRTQNV SQVQRALLLP QEVIQLPRDE QIVLIESFPP IKSRKIKYYE DKFFTSRLLP PTFVPTQVPF NHVSNNSDAS KETETTTAIE NNE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa attttcaaag aataaaatca ttacaatttt ttgagcagcc tgaaaaagta tatggtgacg ctagttgggc taatcagtct gatatagaag ctgctggtct tagatcaaaa aaaggcatgt taataggtgt tgatgctggt ggatattttg ttgcagatgg gtttcagcat gctttattat tcgcacctac cggttcaggt aaaggtgttg gttttgtaat acctaacctt ttattttgga ctgattcagt agtagtacat gatataaagc ttgaaaatca caatttgact agtggttggc gtgagaaaca agggcaaaaa gtttttgttt gggaaccttc taatccagat ggtattacac attgttataa tccgattgat tgggttagta ctaagcctgg gcaaatggtt gatgatgtac agaaaatttc gaatcttata atgccagaaa aggatttttg gaacaatgaa gcacgtagtt tattcttagg cgtaacttta tatttaatag ctgatcctac taaaactaaa tctttcggtg aagtagtacg tacaatgaga agtgacgatg tagtttataa tctagccgtt gtacttgata cgcttggtgg tgtaatacat cctgttgcat atatgaatat tgctgcattt ttgcaaaaag ctgataaaga gcgttccggt gtaatatcta caatgaattc atcacttgaa ttatgggcaa atccacttat tgattctgct actgcatctt ctgacttcaa tatacaagaa tttaaaaaag ttaaaacaac agtgtatgta ggattaacac ctgataatat acagcgtttg caaaaattaa tgcaggtatt ttatcagcag gctacagaat ttttaagccg taaaatgcct gatttaaagg aggaaccata tggagtaatg ttcttacttg atgagttccc gactcttgga aagatggata cttttaaagc tggtatcgct tattttagag gttatagagt tcggttattt ttgattatcc aagatacgca gcaattaaaa ggaacatatg aggacgccgg tatgaattct ttcttatcta attccacata tcgcattacc tttgctgcta ataactatga aactgcaaat ttaatatcgc agcttgttgg taataagact gtagaacaaa ggtcatttag taaaccttta ttttttgacc ttaatatttc tactagaacc caaaatgtat ctcaagttca aagagcttta cttttacctc aagaagtaat acagttaccg cgagatgagc aaattgtttt aattgaatcc ttcccgccta taaaatctcg taaaattaag tattacgaag ataagttttt tactagtaga ttattaccgc cgacttttgt acctactcaa gtacccttta atcatgtgtc aaataatagt gatgcttcta aagagactga aacaacaact gctatcgaaa ataatgagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for RityA.18398.a.B21.GE38034
HARVESTED ON: 5/12/2014
SEQUENCED ON: 5/4/2014
EXPECTED MW: 57kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 88
Validated AA Sequence
MLIGVDAGGY FVADGFQHAL LFAPTGSGKG VGFVIPNLLF WTDSVVVHDI KLENHNLTSG WREKQGQKVF VWEPSNPDGI THCYNPIDWV STKPGQMVDD VQKISNLIMP EKDFWNNEAR SLFLGVTLYL IADPTKTKSF GEVVRTMRSD DVVYNLAVVL DTLGGVIHPV AYMNIAAFLQ KADKERSGVI STMNSSLELW ANPLIDSATA SSDFNIQEFK KVKTTVYVGL TPDNIQRLQK LMQVFYQQAT EFLSRKMPDL KEEPYGVMFL LDEFPTLGKM DTFKAGIAYF RGYRVRLFLI IQDTQQLKGT YEDAGMNSFL SNSTYRITFA ANNYETANLI SQLVGNKTVE QRSFSKPLFF DLNISTRTQN VSQVQRALLL PQEVIQLPRD EQIVLIESFP PIKSRKIKYY EDKFFTSRLL PPTFVPTQVP FNHVSNNSDA SKETE
Validated NT Sequence
gtttcagtct ctttagaagc atcactatta tttgacacat gattaaaggg tacttgagta ggtacaaaag tcggcggtaa taatctacta gtaaaaaact tatcttcgta atacttaatt ttacgagatt ttataggcgg gaaggattca attaaaacaa tttgctcatc tcgcggtaac tgtattactt cttgaggtaa aagtaaagct ctttgaactt gagatacatt ttgggttcta gtagaaatat taaggtcaaa aaataaaggt ttactaaatg acctttgttc tacagtctta ttaccaacaa gctgcgatat taaatttgca gtttcatagt tattagcagc aaaggtaatg cgatatgtgg aattagataa gaaagaattc ataccggcgt cctcatatgt tccttttaat tgctgcgtat cttggataat caaaaataac cgaactctat aacctctaaa ataagcgata ccagctttaa aagtatccat ctttccaaga gtcgggaact catcaagtaa gaacattact ccatatggtt cctcctttaa atcaggcatt ttacggctta aaaattctgt agcctgctga taaaatacct gcattaattt ttgcaaacgc tgtatattat caggtgttaa tcctacatac actgttgttt taactttttt aaattcttgt atattgaagt cagaagatgc agtagcagaa tcaataagtg gatttgccca taattcaagt gatgaattca ttgtagatat tacaccggaa cgctctttat cagctttttg caaaaatgca gcaatattca tatatgcaac aggatgtatt acaccaccaa gcgtatcaag tacaacggct agattataaa ctacatcgtc acttctcatt gtacgtacta cttcaccgaa agatttagtt ttagtaggat cagctattaa atataaagtt acgcctaaga ataaactacg tgcttcattg ttccaaaaat ccttttctgg cattataaga ttcgaaattt tctgtacatc atcaaccatt tgcccaggct tagtactaac ccaatcaatc ggattataac aatgtgtaat accatctgga ttagaaggtt cccaaacaaa aactttttgc ccttgtttct cacgccaacc actagtcaaa ttgtgatttt caagctttat atcatgtact actactgaat cagtccaaaa taaaaggtta ggtattacaa aaccaacacc tttacctgaa ccggtaggtg cgaataataa agcatgctga aacccatctg caacaaaata tccaccagca tcaacaccta ttaacatgcc tttttttgat ctaagaccag cagcttctat atcagactga ttagcccaac tagcgtcacc atatactttt tcaggctgct caaaaaattg taatgatttt attctttgaa aattt
Expected Protein Sequence
MAHHHHHHKN FQRIKSLQFF EQPEKVYGDA SWANQSDIEA AGLRSKKGML IGVDAGGYFV ADGFQHALLF APTGSGKGVG FVIPNLLFWT DSVVVHDIKL ENHNLTSGWR EKQGQKVFVW EPSNPDGITH CYNPIDWVST KPGQMVDDVQ KISNLIMPEK DFWNNEARSL FLGVTLYLIA DPTKTKSFGE VVRTMRSDDV VYNLAVVLDT LGGVIHPVAY MNIAAFLQKA DKERSGVIST MNSSLELWAN PLIDSATASS DFNIQEFKKV KTTVYVGLTP DNIQRLQKLM QVFYQQATEF LSRKMPDLKE EPYGVMFLLD EFPTLGKMDT FKAGIAYFRG YRVRLFLIIQ DTQQLKGTYE DAGMNSFLSN STYRITFAAN NYETANLISQ LVGNKTVEQR SFSKPLFFDL NISTRTQNVS QVQRALLLPQ EVIQLPRDEQ IVLIESFPPI KSRKIKYYED KFFTSRLLPP TFVPTQVPFN HVSNNSDASK ETETTTAIEN NE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaaaatt ttcaaagaat aaaatcatta caattttttg agcagcctga aaaagtatat ggtgacgcta gttgggctaa tcagtctgat atagaagctg ctggtcttag atcaaaaaaa ggcatgttaa taggtgttga tgctggtgga tattttgttg cagatgggtt tcagcatgct ttattattcg cacctaccgg ttcaggtaaa ggtgttggtt ttgtaatacc taacctttta ttttggactg attcagtagt agtacatgat ataaagcttg aaaatcacaa tttgactagt ggttggcgtg agaaacaagg gcaaaaagtt tttgtttggg aaccttctaa tccagatggt attacacatt gttataatcc gattgattgg gttagtacta agcctgggca aatggttgat gatgtacaga aaatttcgaa tcttataatg ccagaaaagg atttttggaa caatgaagca cgtagtttat tcttaggcgt aactttatat ttaatagctg atcctactaa aactaaatct ttcggtgaag tagtacgtac aatgagaagt gacgatgtag tttataatct agccgttgta cttgatacgc ttggtggtgt aatacatcct gttgcatata tgaatattgc tgcatttttg caaaaagctg ataaagagcg ttccggtgta atatctacaa tgaattcatc acttgaatta tgggcaaatc cacttattga ttctgctact gcatcttctg acttcaatat acaagaattt aaaaaagtta aaacaacagt gtatgtagga ttaacacctg ataatataca gcgtttgcaa aaattaatgc aggtatttta tcagcaggct acagaatttt taagccgtaa aatgcctgat ttaaaggagg aaccatatgg agtaatgttc ttacttgatg agttcccgac tcttggaaag atggatactt ttaaagctgg tatcgcttat tttagaggtt atagagttcg gttatttttg attatccaag atacgcagca attaaaagga acatatgagg acgccggtat gaattctttc ttatctaatt ccacatatcg cattaccttt gctgctaata actatgaaac tgcaaattta atatcgcagc ttgttggtaa taagactgta gaacaaaggt catttagtaa acctttattt tttgacctta atatttctac tagaacccaa aatgtatctc aagttcaaag agctttactt ttacctcaag aagtaataca gttaccgcga gatgagcaaa ttgttttaat tgaatccttc ccgcctataa aatctcgtaa aattaagtat tacgaagata agttttttac tagtagatta ttaccgccga cttttgtacc tactcaagta ccctttaatc atgtgtcaaa taatagtgat gcttctaaag agactgaaac aacaactgct atcgaaaata atgagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for RityA.18398.a.B22.GE41487
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHKN FQRIKSLQFF EQPEKVYGDA SWANQSDIEA AGLRSKKGML IGVDAGGYFV ADGFQHALLF APTGSGKGVG FVIPNLLFWT DSVVVHDIKL ENHNLTSGWR EKQGQKVFVW EPSNPDGITH CYNPIDWVST KPGQMVDDVQ KISNLIMPEK DFWNNEARSL FLGVTLYLIA DPTKTKSFGE VVRTMRSDDV VYNLAVVLDT LGGVIHPVAY MNIAAFLQKA DKERSGVIST MNSSLELWAN PLIDSATASS DFNIQEFKKV KTTVYVGLTP DNIQRLQKLM QVFYQQATEF LSRKMPDLKE EPYGVMFLLD EFPTLGKMDT FKAGIAYFRG YRVRLFLIIQ DTQQLKGTYE DAGMNSFLSN STYRITFAAN NYETANLISQ LVGNKTVEQR SFSKPLFFDL NISTRTQNVS QVQRALLLPQ EVIQLPRDEQ IVLIESFPPI KSRKIKYYED KFFTSRLLPP TFVPTQVPFN HVSNNSDASK ETETTTAIEN NE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaaaact tccagcgtat caaatcgctg cagtttttcg aacaaccgga aaaagtctat ggcgatgcat catgggctaa tcaatcggac attgaagcgg ccggtctgcg cagcaagaaa ggcatgctga tcggtgttga tgcgggcggt tattttgtcg ccgacggctt ccagcatgca ctgctgtttg ctccgaccgg ctccggtaaa ggcgttggtt ttgtcattcc gaacctgctg ttctggaccg atagtgtggt tgtccatgac atcaaactgg aaaaccacaa tctgacgtcc ggctggcgtg aaaaacaggg tcaaaaagtg tttgtttggg aaccgagcaa cccggatggc attacccact gctacaatcc gatcgactgg gttagcacga aaccgggtca gatggtggat gacgttcaaa aaatctctaa cctgatcatg ccggaaaaag atttttggaa caatgaagca cgctcactgt tcctgggcgt gaccctgtat ctgattgctg acccgaccaa aacgaaatca tttggtgaag tggttcgtac catgcgctcg gatgacgtcg tgtataacct ggccgttgtc ctggatacgc tgggcggtgt tattcatccg gtcgcgtaca tgaatatcgc agctttcctg cagaaagccg acaaagaacg cagtggcgtg atttccacca tgaacagctc tctggaactg tgggcaaatc cgctgattga tagtgcaacc gctagttccg actttaacat ccaggaattc aagaaagtga aaaccacggt ctatgtgggt ctgaccccgg ataatatcca gcgtctgcaa aaactgatgc aggtgtttta ccagcaagcg accgaattcc tgtctcgcaa aatgccggat ctgaaagaag aaccgtatgg cgttatgttt ctgctggatg aattcccgac cctgggtaaa atggacacgt ttaaagcggg cattgcctat ttccgtggtt accgtgtgcg cctgtttctg attatccagg atacccagca actgaaaggc acgtatgaag acgccggtat gaacagcttt ctgtcaaatt cgacctatcg tattacgttc gcggccaaca attacgaaac cgctaacctg atctctcagc tggtcggtaa taaaacggtg gaacaacgca gcttttctaa accgctgttt ttcgatctga acattagtac ccgtacgcag aatgtctccc aggtgcaacg tgcactgctg ctgccgcagg aagtgatcca actgccgcgc gatgaacaga ttgttctgat cgaatctttt ccgccgatta aatctcgtaa aatcaaatac tacgaagata aatttttcac cagccgtctg ctgccgccga cctttgtgcc gacgcaggtt ccgttcaatc acgtgagtaa caacagtgac gcaagcaaag aaaccgaaac cacgaccgct attgaaaaca acgaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for RityA.18398.a.B42.GE41488
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 91
PERCENT COVERAGE: 50
Validated AA Sequence
MAHHHHHHMK SLQFFEQPEK VYGDASWANQ SDIEAAGLRS KKGMLIGVDA GGYFVADGFQ HALLFAPTGS GKGVGFVIPN LLFWTDSVVV HDIKLENHNL TSGWREKQGQ KVFVWEPSNP DGITHCYNPI DWVSTKPGQM VDDVQKISNL IMPEKDFWNN EARSLFLGVT LYLIADPTKT KSFGEVVRTM RSDDVVYNLA VVLDTLGGVI HPVAYMNIAA FPAESRQRTQ WRDFHHEQLS GTVGKSAD
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa tcgctgcagt ttttcgaaca accggaaaaa gtctatggcg atgcatcatg ggctaatcaa tcggacattg aagcggccgg tctgcgcagc aagaaaggca tgctgatcgg tgttgatgcg ggcggttatt ttgtcgccga cggcttccag catgcactgc tgtttgctcc gaccggctcc ggtaaaggcg ttggttttgt cattccgaac ctgctgttct ggaccgatag tgtggttgtc catgacatca aactggaaaa ccacaatctg acgtccggct ggcgtgaaaa acagggtcaa aaagtgtttg tttgggaacc gagcaacccg gatggcatta cccactgcta caatccgatc gactgggtta gcacgaaacc gggtcagatg gtggatgacg ttcaaaaaat ctctaacctg atcatgccgg aaaaagattt ttggaacaat gaagcacgct cactgttcct gggcgtgacc ctgtatctga ttgctgaccc gaccaaaacg aaatcatttg gtgaagtggt tcgtaccatg cgctcggatg acgtcgtgta taacctggcc gttgtcctgg atacgctggg cggtgttatt catccggtcg cgtacatgaa tatcgcagct ttccctgcag aaagccgaca aagaacgcag tggcgtgatt tccaccatga acagctctct ggaactgtgg gcaaatccgc tgattgatag tgcaaccgct agttccgact ttaacatcca ggaattcaag aaagtgaaaa ccacggtcta tgtgggtctg accccggata atatccagcg tctgcaaaaa ctgatgcagg tgttttacca gcaagcgacc gaattcctgt ctcgcaaaat gccggatctg aaagaagaac cgtatggcgt tatgtttctg ctggatgaat tcccgaccct gggtaaaatg gacacgttta aagcgggcat tgcctatttc cgtggttacc gtgtgcgcct gtttctgatt atccaggata cccagcaact gaaaggcacg tatgaagacg ccggtatgaa cagctttctg tcaaattcga cctatcgtat tacgttcgcg gccaacaatt acgaaaccgc taacctgatc tctcagctgg tcggtaataa aacggtggaa caacgcagct tttctaaacc gctgtttttc gatctgaaca ttagtacccg tacgcagaat gtctcccagg tgcaacgtgc actgctgctg ccgcaggaag tgatccaact gccgcgcgat gaacagattg ttctgatcga atcttttccg ccgattaaat ctcgtaaaat caaatactac gaagataaat ttttcaccag ccgtctgctg ccgccgacct ttgtgccgac gcaggttccg ttcaatcacg tgagtaacaa cagtgacgca agcaaagaaa ccgaaacc
Expected Protein Sequence
MAHHHHHHKS LQFFEQPEKV YGDASWANQS DIEAAGLRSK KGMLIGVDAG GYFVADGFQH ALLFAPTGSG KGVGFVIPNL LFWTDSVVVH DIKLENHNLT SGWREKQGQK VFVWEPSNPD GITHCYNPID WVSTKPGQMV DDVQKISNLI MPEKDFWNNE ARSLFLGVTL YLIADPTKTK SFGEVVRTMR SDDVVYNLAV VLDTLGGVIH PVAYMNIAAF LQKADKERSG VISTMNSSLE LWANPLIDSA TASSDFNIQE FKKVKTTVYV GLTPDNIQRL QKLMQVFYQQ ATEFLSRKMP DLKEEPYGVM FLLDEFPTLG KMDTFKAGIA YFRGYRVRLF LIIQDTQQLK GTYEDAGMNS FLSNSTYRIT FAANNYETAN LISQLVGNKT VEQRSFSKPL FFDLNISTRT QNVSQVQRAL LLPQEVIQLP RDEQIVLIES FPPIKSRKIK YYEDKFFTSR LLPPTFVPTQ VPFNHVSNNS DASKETETTT AIENNE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaatcgc tgcagttttt cgaacaaccg gaaaaagtct atggcgatgc atcatgggct aatcaatcgg acattgaagc ggccggtctg cgcagcaaga aaggcatgct gatcggtgtt gatgcgggcg gttattttgt cgccgacggc ttccagcatg cactgctgtt tgctccgacc ggctccggta aaggcgttgg ttttgtcatt ccgaacctgc tgttctggac cgatagtgtg gttgtccatg acatcaaact ggaaaaccac aatctgacgt ccggctggcg tgaaaaacag ggtcaaaaag tgtttgtttg ggaaccgagc aacccggatg gcattaccca ctgctacaat ccgatcgact gggttagcac gaaaccgggt cagatggtgg atgacgttca aaaaatctct aacctgatca tgccggaaaa agatttttgg aacaatgaag cacgctcact gttcctgggc gtgaccctgt atctgattgc tgacccgacc aaaacgaaat catttggtga agtggttcgt accatgcgct cggatgacgt cgtgtataac ctggccgttg tcctggatac gctgggcggt gttattcatc cggtcgcgta catgaatatc gcagctttcc tgcagaaagc cgacaaagaa cgcagtggcg tgatttccac catgaacagc tctctggaac tgtgggcaaa tccgctgatt gatagtgcaa ccgctagttc cgactttaac atccaggaat tcaagaaagt gaaaaccacg gtctatgtgg gtctgacccc ggataatatc cagcgtctgc aaaaactgat gcaggtgttt taccagcaag cgaccgaatt cctgtctcgc aaaatgccgg atctgaaaga agaaccgtat ggcgttatgt ttctgctgga tgaattcccg accctgggta aaatggacac gtttaaagcg ggcattgcct atttccgtgg ttaccgtgtg cgcctgtttc tgattatcca ggatacccag caactgaaag gcacgtatga agacgccggt atgaacagct ttctgtcaaa ttcgacctat cgtattacgt tcgcggccaa caattacgaa accgctaacc tgatctctca gctggtcggt aataaaacgg tggaacaacg cagcttttct aaaccgctgt ttttcgatct gaacattagt acccgtacgc agaatgtctc ccaggtgcaa cgtgcactgc tgctgccgca ggaagtgatc caactgccgc gcgatgaaca gattgttctg atcgaatctt ttccgccgat taaatctcgt aaaatcaaat actacgaaga taaatttttc accagccgtc tgctgccgcc gacctttgtg ccgacgcagg ttccgttcaa tcacgtgagt aacaacagtg acgcaagcaa agaaaccgaa accacgaccg ctattgaaaa caacgaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at