RityA.18390.a
VirB8-like protein of type IV secretion system
| CENTER ID: | RityA.18390.a |
| ORGANISM: | Rickettsia typhi str. Wilmington |
| ASSOCIATED DISEASE: | Murine typhus (endemic typhus) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RityA.18390.a.B2.GE35076 | virB domain | 61 | 232 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RityA.18390.a.B2.PS01633 | virB domain | 61 | 232 |
Structures
4O3V
DEPOSITED: 12/18/2013
DETERMINATION: XRay
CLONE: RityA.18390.a.B2.GE35076
PROTEIN: RityA.18390.a.B2.PS01633
7SH3
DEPOSITED: 10/7/2021
DETERMINATION: XRay
CLONE: RityA.18390.a.B2.GE35076
PROTEIN: RityA.18390.a.B2.PS38656
Publications by SSGCID
Structural Insight into How Bacteria Prevent Interference between Multiple Divergent Type IV Secretion Systems.
Azad AF, Edwards TE, Gillespie J, Lehman SS, Myler PJ, Phan IQ, Piitulainen H, Pulliainen A, Rennoll-Bankert KE, Sayeedur Rahman M, Scheib H, Stacy R, Staker BL, Subramanian S, Taira S
MBio - 2015
volume 6, issue 6, pages e01867-15
PMID: 26646013; PMCID: PMC4676284
The Rickettsia type IV secretion system: unrealized complexity mired by gene family expansion
Azad AF, Beier-Sexton M, Driscoll TP, Gillespie J, Guilotte ML, Lehman SS, Myler PJ, Phan I, Rennoll-Bankert KE, Sayeedur Rahman M, Subramanian S
Pathog Dis - 2016
volume 74, issue 6, pages ftw058
PMID: 27307105; PMCID: PMC5505475
PDB:
4O3V
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_178593 |
| PATRIC ID: | fig|257363.4.peg.286 |
| RefSeq: | YP_067240.1 |
| UniProt: | Q68X84 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDQLTNAIKE YIKSGEYFID ARKWYNFKYI LPLSHRSFLL LTCTIFTLLL TLIGVNINIL LPIQKKVGYL IKDDSEKQAT ITNTKYSTLA NPYISVANIM LQNYVKQREK YNYDTLKEQF TFIKNASTSI VYMQFANFMN IDNSLSPVIR YQKLYRRSIN IISINNINNN EATVTFESLA QNNTGEILEN MLWEAKIGFI MDSISTSTLH NMPFHFIVTS YKLKLLRNKN QQ
NT Sequence
atggaccaat taacaaatgc aataaaagaa tatataaaat ccggtgaata ttttattgat gcgagaaaat ggtataattt taagtatatc ttaccgttaa gtcatagaag ttttctactc ttaacatgca caatttttac attattatta acattaattg gtgtaaatat caacatattg ttaccaatac agaaaaaggt aggttattta ataaaagatg attcagaaaa acaagctacc attactaata ctaaatattc aacattagca aatccttata tttctgttgc aaatattatg cttcagaatt atgtaaaaca acgagaaaaa tacaattatg atacattaaa agaacaattc acttttatta aaaatgcttc tacgagtatt gtttatatgc aatttgctaa ttttatgaat attgataact cattatcacc tgtcatacga tatcaaaagc tatatagacg ctcaattaat ataatatcaa ttaacaatat caataataac gaggcaactg ttacttttga atcattggca caaaataata ctggtgaaat cttagaaaat atgctatggg aagcaaaaat tggatttata atggattcta tcagtacaag tacattacac aatatgccgt tccattttat agttactagt tacaagctaa aattattaag aaataaaaat cagcaataa
Details for RityA.18390.a.B2.GE35076
| HARVESTED ON: | 4/10/2012 |
| SEQUENCED ON: | 1/18/2018 |
| EXPECTED MW: | 21kDa |
| OBSERVED MW: | 20kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML PIQKKVGYLI KDDSEKQATI TNTKYSTLAN PYISVANIML QNYVKQREKY NYDTLKEQFT FIKNASTSIV YMQFANFMNI DNSLSPVIRY QKLYRRSINI ISINNINNNE ATVTFESLAQ NNTGEILENM LWEAKIGFIM DSISTSTLHN MPFHFIVTSY KLKLLRNKNQ Q
Validated NT Sequence
atcttactca cttattgctg atttttattt cttaataatt ttagcttgta actagtaact ataaaatgga acggcatatt gtgtaatgta cttgtactga tagaatccat tataaatcca atttttgctt cccatagcat attttctaag atttcaccag tattattttg tgccaatgat tcaaaagtaa cagttgcctc gttattattg atattgttaa ttgatattat attaattgag cgtctatata gcttttgata tcgtatgaca ggtgataatg agttatcaat attcataaaa ttagcaaatt gcatataaac aatactcgta gaagcatttt taataaaagt gaattgttct tttaatgtat cataattgta tttttctcgt tgttttacat aattctgaag cataatattt gcaacagaaa tataaggatt tgctaatgtt gaatatttag tattagtaat ggtagcttgt ttttctgaat catcttttat taaataacct acctttttct gtattggtaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHML PIQKKVGYLI KDDSEKQATI TNTKYSTLAN PYISVANIML QNYVKQREKY NYDTLKEQFT FIKNASTSIV YMQFANFMNI DNSLSPVIRY QKLYRRSINI ISINNINNNE ATVTFESLAQ NNTGEILENM LWEAKIGFIM DSISTSTLHN MPFHFIVTSY KLKLLRNKNQ Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttac caatacagaa aaaggtaggt tatttaataa aagatgattc agaaaaacaa gctaccatta ctaatactaa atattcaaca ttagcaaatc cttatatttc tgttgcaaat attatgcttc agaattatgt aaaacaacga gaaaaataca attatgatac attaaaagaa caattcactt ttattaaaaa tgcttctacg agtattgttt atatgcaatt tgctaatttt atgaatattg ataactcatt atcacctgtc atacgatatc aaaagctata tagacgctca attaatataa tatcaattaa caatatcaat aataacgagg caactgttac ttttgaatca ttggcacaaa ataatactgg tgaaatctta gaaaatatgc tatgggaagc aaaaattgga tttataatgg attctatcag tacaagtaca ttacacaata tgccgttcca ttttatagtt actagttaca agctaaaatt attaagaaat aaaaatcagc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for RityA.18390.a.B2.PS01633
| PURIFICATION DATe: | 9/24/2012 |
| CONCENTRATION: | 11.2mg/ml |
| OBSERVED MW: | 18kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML PIQKKVGYLI KDDSEKQATI TNTKYSTLAN PYISVANIML QNYVKQREKY NYDTLKEQFT FIKNASTSIV YMQFANFMNI DNSLSPVIRY QKLYRRSINI ISINNINNNE ATVTFESLAQ NNTGEILENM LWEAKIGFIM DSISTSTLHN MPFHFIVTSY KLKLLRNKNQ Q
Validated NT Sequence
atcttactca cttattgctg atttttattt cttaataatt ttagcttgta actagtaact ataaaatgga acggcatatt gtgtaatgta cttgtactga tagaatccat tataaatcca atttttgctt cccatagcat attttctaag atttcaccag tattattttg tgccaatgat tcaaaagtaa cagttgcctc gttattattg atattgttaa ttgatattat attaattgag cgtctatata gcttttgata tcgtatgaca ggtgataatg agttatcaat attcataaaa ttagcaaatt gcatataaac aatactcgta gaagcatttt taataaaagt gaattgttct tttaatgtat cataattgta tttttctcgt tgttttacat aattctgaag cataatattt gcaacagaaa tataaggatt tgctaatgtt gaatatttag tattagtaat ggtagcttgt ttttctgaat catcttttat taaataacct acctttttct gtattggtaa catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHML PIQKKVGYLI KDDSEKQATI TNTKYSTLAN PYISVANIML QNYVKQREKY NYDTLKEQFT FIKNASTSIV YMQFANFMNI DNSLSPVIRY QKLYRRSINI ISINNINNNE ATVTFESLAQ NNTGEILENM LWEAKIGFIM DSISTSTLHN MPFHFIVTSY KLKLLRNKNQ Q
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAC CAATACAGAA AAAGGTAGGT TATTTAATAA AAGATGATTC AGAAAAACAA GCTACCATTA CTAATACTAA ATATTCAACA TTAGCAAATC CTTATATTTC TGTTGCAAAT ATTATGCTTC AGAATTATGT AAAACAACGA GAAAAATACA ATTATGATAC ATTAAAAGAA CAATTCACTT TTATTAAAAA TGCTTCTACG AGTATTGTTT ATATGCAATT TGCTAATTTT ATGAATATTG ATAACTCATT ATCACCTGTC ATACGATATC AAAAGCTATA TAGACGCTCA ATTAATATAA TATCAATTAA CAATATCAAT AATAACGAGG CAACTGTTAC TTTTGAATCA TTGGCACAAA ATAATACTGG TGAAATCTTA GAAAATATGC TATGGGAAGC AAAAATTGGA TTTATAATGG ATTCTATCAG TACAAGTACA TTACACAATA TGCCGTTCCA TTTTATAGTT ACTAGTTACA AGCTAAAATT ATTAAGAAAT AAAAATCAGC AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT