RityA.17987.a
DNA polymerase III subunit beta (EC 2.7.7.7)
| CENTER ID: | RityA.17987.a |
| ORGANISM: | Rickettsia typhi str. Wilmington |
| ASSOCIATED DISEASE: | Murine typhus (endemic typhus) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RityA.17987.a.B1.GE41369 | full length | 1 | 381 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RityA.17987.a.B1.PW38354 | full length | 1 | 381 |
Structures
6D46
DEPOSITED: 4/17/2018
DETERMINATION: XRay
CLONE: RityA.17987.a.B1.GE41369
PROTEIN: RityA.17987.a.B1.PW38354
6DJK
DEPOSITED: 5/25/2018
DETERMINATION: XRay
CLONE: RityA.17987.a.B1.GE41369
PROTEIN: RityA.17987.a.B1.PW38354
Publications by SSGCID
Exquisite selectivity of griselimycin extends to beta subunit of DNA polymerases from Gram-negative bacterial pathogens
Abendroth JA, Barrett L, Choi R, Dranow DM, Edwards TE, Hewitt SN, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Subramanian S, Udell HS, Van Voorhis W, Wallace E, Mayclin SJ, Hammerson B, Chun IKH, Barrett KF, Conrady DG, Mueller R, Tillery L, Lukat P, Blankenfeldt W, Higgins T, Bowatte K, Smutney H, Stigliano M, Fenwick MK, Craig J, Peng V, DeRocher AE
Nat Commun - 2024
volume 7, issue 1,
PMID: 39639052; PMCID: PMC11621423
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|257363.4.peg.434 |
| UniProt: | Q68WW0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKLIVETKT LVQSLGFARS VVEKRNVIPE YANIKLSAKD GNLELSSTNM DLYLSQKIAV QVLSEGEITV STQTLSDIVR KLPDSELTLT ELDIMKLEIK GQNCQFNLFT LPVSSFPAMD SIKPEVSFKI SCADFAKIIE STKFSISLDE TRYNLNGIYL HIKDKEFFAA STDGHRLSIS WITLEEKIKN FGVILPQKSA EEILKIVKDL KNIHEDIEIL LSSNKIKFIC NENTILLSKL IDGTFPDYSA FIPKSSISKL VINRKIFADS IERIAIITVE KFRAIKLSLS RKTLEISAVG EARGNAKEII TASQDKESFY EYNCDESLVI GFNPQYLEDV LKAVKSNLVE LYFSDISASA PVLIKFPQNP KDIFVIMPVK V
NT Sequence
atgttaaaat taatcgttga aacgaaaacg ttagtacagt ccctcggttt tgcaagatct gttgttgaaa aacgtaatgt aatacctgaa tatgcaaaca taaaattatc agcaaaagat ggcaatctag agcttagttc tactaatatg gatttgtact taagtcaaaa aattgcagta caggtattaa gtgaagggga aattaccgtt tctacacaaa ctctaagtga tatagtccgc aaactgccag attctgagct tacattaaca gaactagata taatgaaact tgaaatcaaa ggtcaaaact gtcaatttaa tttatttact ttacctgtta gttcttttcc tgcgatggat agtattaaac ctgaagtaag ctttaaaatt tcttgtgcgg attttgctaa aataatcgaa tcaacaaaat tttcaatttc tttagatgaa actcgttata atttaaatgg tatttattta cacataaaag ataaagaatt ttttgcagca agcactgatg ggcatagact ttcaatttca tggataacat tggaagaaaa aataaagaat tttggagtta tattacctca aaaaagtgca gaagaaattt taaaaatagt taaagatctt aaaaatatac atgaagacat agaaatttta ttaagtagta ataaaattaa atttatatgt aatgaaaata ctattttact gtcaaagctt atagatggga cttttcctga ttatagtgct tttattccga aaagcagcat atcaaaatta gtgattaatc gaaaaatatt tgcagatagt atagaaagga tagcgataat aaccgttgag aaatttagag cgatcaaatt atcattatct cgtaagacct tagagattag tgctgtcgga gaagcaagag gcaacgcaaa agagattatt actgcctcac aagataaaga aagtttctat gaatataatt gtgatgagtc tttagtcata ggctttaatc cacaatactt agaagatgtt ttaaaagcag taaaatcgaa tttagtagaa ttatatttct cagatatttc agcttcagca ccagtactta ttaaatttcc tcaaaatcct aaagatattt ttgtaatcat gcctgttaag gtttaa
Details for RityA.17987.a.B1.GE41369
| HARVESTED ON: | 4/3/2017 |
| SEQUENCED ON: | 4/10/2017 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHML KLIVETKTLV QSLGFARSVV EKRNVIPEYA NIKLSAKDGN LELSSTNMDL YLSQKIAVQV LSEGEITVST QTLSDIVRKL PDSELTLTEL DIMKLEIKGQ NCQFNLFTLP VSSFPAMDSI KPEVSFKISC ADFAKIIEST KFSISLDETR YNLNGIYLHI KDKEFFAAST DGHRLSISWI TLEEKIKNFG VILPQKSAEE ILKIVKDLKN IHEDIEILLS SNKIKFICNE NTILLSKLID GTFPDYSAFI PKSSISKLVI NRKIFADSIE RIAIITVEKF RAIKLSLSRK TLEISAVGEA RGNAKEIITA SQDKESFYEY NCDESLVIGF NPQYLEDVLK AVKSNLVELY FSDISASAPV LIKFPQNPKD I
Validated NT Sequence
aaaaatatct ttaggatttt gaggaaattt aataagtact ggtgctgaag ctgaaatatc tgagaaatat aattctacta aattcgattt tactgctttt aaaacatctt ctaagtattg tggattaaag cctatgacta aagactcatc acaattatat tcatagaaac tttctttatc ttgtgaggca gtaataatct cttttgcgtt gcctcttgct tctccgacag cactaatctc taaggtctta cgagataatg ataatttgat cgctctaaat ttctcaacgg ttattatcgc tatcctttct atactatctg caaatatttt tcgattaatc actaattttg atatgctgct tttcggaata aaagcactat aatcaggaaa agtcccatct ataagctttg acagtaaaat agtattttca ttacatataa atttaatttt attactactt aataaaattt ctatgtcttc atgtatattt ttaagatctt taactatttt taaaatttct tctgcacttt tttgaggtaa tataactcca aaattcttta ttttttcttc caatgttatc catgaaattg aaagtctatg cccatcagtg cttgctgcaa aaaattcttt atcttttatg tgtaaataaa taccatttaa attataacga gtttcatcta aagaaattga aaattttgtt gattcgatta ttttagcaaa atccgcacaa gaaattttaa agcttacttc aggtttaata ctatccatcg caggaaaaga actaacaggt aaagtaaata aattaaattg acagttttga cctttgattt caagtttcat tatatctagt tctgttaatg taagctcaga atctggcagt ttgcggacta tatcacttag agtttgtgta gaaacggtaa tttccccttc acttaatacc tgtactgcaa ttttttgact taagtacaaa tccatattag tagaactaag ctctagattg ccatcttttg ctgataattt tatgtttgca tattcaggta ttacattacg tttttcaaca acagatcttg caaaaccgag ggactgtact aacgttttcg tttcaacgat taattttaac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHML KLIVETKTLV QSLGFARSVV EKRNVIPEYA NIKLSAKDGN LELSSTNMDL YLSQKIAVQV LSEGEITVST QTLSDIVRKL PDSELTLTEL DIMKLEIKGQ NCQFNLFTLP VSSFPAMDSI KPEVSFKISC ADFAKIIEST KFSISLDETR YNLNGIYLHI KDKEFFAAST DGHRLSISWI TLEEKIKNFG VILPQKSAEE ILKIVKDLKN IHEDIEILLS SNKIKFICNE NTILLSKLID GTFPDYSAFI PKSSISKLVI NRKIFADSIE RIAIITVEKF RAIKLSLSRK TLEISAVGEA RGNAKEIITA SQDKESFYEY NCDESLVIGF NPQYLEDVLK AVKSNLVELY FSDISASAPV LIKFPQNPKD IFVIMPVKV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa aattaatcgt tgaaacgaaa acgttagtac agtccctcgg ttttgcaaga tctgttgttg aaaaacgtaa tgtaatacct gaatatgcaa acataaaatt atcagcaaaa gatggcaatc tagagcttag ttctactaat atggatttgt acttaagtca aaaaattgca gtacaggtat taagtgaagg ggaaattacc gtttctacac aaactctaag tgatatagtc cgcaaactgc cagattctga gcttacatta acagaactag atataatgaa acttgaaatc aaaggtcaaa actgtcaatt taatttattt actttacctg ttagttcttt tcctgcgatg gatagtatta aacctgaagt aagctttaaa atttcttgtg cggattttgc taaaataatc gaatcaacaa aattttcaat ttctttagat gaaactcgtt ataatttaaa tggtatttat ttacacataa aagataaaga attttttgca gcaagcactg atgggcatag actttcaatt tcatggataa cattggaaga aaaaataaag aattttggag ttatattacc tcaaaaaagt gcagaagaaa ttttaaaaat agttaaagat cttaaaaata tacatgaaga catagaaatt ttattaagta gtaataaaat taaatttata tgtaatgaaa atactatttt actgtcaaag cttatagatg ggacttttcc tgattatagt gcttttattc cgaaaagcag catatcaaaa ttagtgatta atcgaaaaat atttgcagat agtatagaaa ggatagcgat aataaccgtt gagaaattta gagcgatcaa attatcatta tctcgtaaga ccttagagat tagtgctgtc ggagaagcaa gaggcaacgc aaaagagatt attactgcct cacaagataa agaaagtttc tatgaatata attgtgatga gtctttagtc ataggcttta atccacaata cttagaagat gttttaaaag cagtaaaatc gaatttagta gaattatatt tctcagatat ttcagcttca gcaccagtac ttattaaatt tcctcaaaat cctaaagata tttttgtaat catgcctgtt aaggtttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for RityA.17987.a.B1.PW38354
| PURIFICATION DATe: | 9/19/2017 |
| CONCENTRATION: | 24.49mg/ml |
| OBSERVED MW: | 49kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
Combined pellet with 43166
Validated AA Sequence
MAHHHHHHML KLIVETKTLV QSLGFARSVV EKRNVIPEYA NIKLSAKDGN LELSSTNMDL YLSQKIAVQV LSEGEITVST QTLSDIVRKL PDSELTLTEL DIMKLEIKGQ NCQFNLFTLP VSSFPAMDSI KPEVSFKISC ADFAKIIEST KFSISLDETR YNLNGIYLHI KDKEFFAAST DGHRLSISWI TLEEKIKNFG VILPQKSAEE ILKIVKDLKN IHEDIEILLS SNKIKFICNE NTILLSKLID GTFPDYSAFI PKSSISKLVI NRKIFADSIE RIAIITVEKF RAIKLSLSRK TLEISAVGEA RGNAKEIITA SQDKESFYEY NCDESLVIGF NPQYLEDVLK AVKSNLVELY FSDISASAPV LIKFPQNPKD I
Validated NT Sequence
aaaaatatct ttaggatttt gaggaaattt aataagtact ggtgctgaag ctgaaatatc tgagaaatat aattctacta aattcgattt tactgctttt aaaacatctt ctaagtattg tggattaaag cctatgacta aagactcatc acaattatat tcatagaaac tttctttatc ttgtgaggca gtaataatct cttttgcgtt gcctcttgct tctccgacag cactaatctc taaggtctta cgagataatg ataatttgat cgctctaaat ttctcaacgg ttattatcgc tatcctttct atactatctg caaatatttt tcgattaatc actaattttg atatgctgct tttcggaata aaagcactat aatcaggaaa agtcccatct ataagctttg acagtaaaat agtattttca ttacatataa atttaatttt attactactt aataaaattt ctatgtcttc atgtatattt ttaagatctt taactatttt taaaatttct tctgcacttt tttgaggtaa tataactcca aaattcttta ttttttcttc caatgttatc catgaaattg aaagtctatg cccatcagtg cttgctgcaa aaaattcttt atcttttatg tgtaaataaa taccatttaa attataacga gtttcatcta aagaaattga aaattttgtt gattcgatta ttttagcaaa atccgcacaa gaaattttaa agcttacttc aggtttaata ctatccatcg caggaaaaga actaacaggt aaagtaaata aattaaattg acagttttga cctttgattt caagtttcat tatatctagt tctgttaatg taagctcaga atctggcagt ttgcggacta tatcacttag agtttgtgta gaaacggtaa tttccccttc acttaatacc tgtactgcaa ttttttgact taagtacaaa tccatattag tagaactaag ctctagattg ccatcttttg ctgataattt tatgtttgca tattcaggta ttacattacg tttttcaaca acagatcttg caaaaccgag ggactgtact aacgttttcg tttcaacgat taattttaac atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHML KLIVETKTLV QSLGFARSVV EKRNVIPEYA NIKLSAKDGN LELSSTNMDL YLSQKIAVQV LSEGEITVST QTLSDIVRKL PDSELTLTEL DIMKLEIKGQ NCQFNLFTLP VSSFPAMDSI KPEVSFKISC ADFAKIIEST KFSISLDETR YNLNGIYLHI KDKEFFAAST DGHRLSISWI TLEEKIKNFG VILPQKSAEE ILKIVKDLKN IHEDIEILLS SNKIKFICNE NTILLSKLID GTFPDYSAFI PKSSISKLVI NRKIFADSIE RIAIITVEKF RAIKLSLSRK TLEISAVGEA RGNAKEIITA SQDKESFYEY NCDESLVIGF NPQYLEDVLK AVKSNLVELY FSDISASAPV LIKFPQNPKD IFVIMPVKV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAA AATTAATCGT TGAAACGAAA ACGTTAGTAC AGTCCCTCGG TTTTGCAAGA TCTGTTGTTG AAAAACGTAA TGTAATACCT GAATATGCAA ACATAAAATT ATCAGCAAAA GATGGCAATC TAGAGCTTAG TTCTACTAAT ATGGATTTGT ACTTAAGTCA AAAAATTGCA GTACAGGTAT TAAGTGAAGG GGAAATTACC GTTTCTACAC AAACTCTAAG TGATATAGTC CGCAAACTGC CAGATTCTGA GCTTACATTA ACAGAACTAG ATATAATGAA ACTTGAAATC AAAGGTCAAA ACTGTCAATT TAATTTATTT ACTTTACCTG TTAGTTCTTT TCCTGCGATG GATAGTATTA AACCTGAAGT AAGCTTTAAA ATTTCTTGTG CGGATTTTGC TAAAATAATC GAATCAACAA AATTTTCAAT TTCTTTAGAT GAAACTCGTT ATAATTTAAA TGGTATTTAT TTACACATAA AAGATAAAGA ATTTTTTGCA GCAAGCACTG ATGGGCATAG ACTTTCAATT TCATGGATAA CATTGGAAGA AAAAATAAAG AATTTTGGAG TTATATTACC TCAAAAAAGT GCAGAAGAAA TTTTAAAAAT AGTTAAAGAT CTTAAAAATA TACATGAAGA CATAGAAATT TTATTAAGTA GTAATAAAAT TAAATTTATA TGTAATGAAA ATACTATTTT ACTGTCAAAG CTTATAGATG GGACTTTTCC TGATTATAGT GCTTTTATTC CGAAAAGCAG CATATCAAAA TTAGTGATTA ATCGAAAAAT ATTTGCAGAT AGTATAGAAA GGATAGCGAT AATAACCGTT GAGAAATTTA GAGCGATCAA ATTATCATTA TCTCGTAAGA CCTTAGAGAT TAGTGCTGTC GGAGAAGCAA GAGGCAACGC AAAAGAGATT ATTACTGCCT CACAAGATAA AGAAAGTTTC TATGAATATA ATTGTGATGA GTCTTTAGTC ATAGGCTTTA ATCCACAATA CTTAGAAGAT GTTTTAAAAG CAGTAAAATC GAATTTAGTA GAATTATATT TCTCAGATAT TTCAGCTTCA GCACCAGTAC TTATTAAATT TCCTCAAAAT CCTAAAGATA TTTTTGTAAT CATGCCTGTT AAGGTTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T