RityA.17851.a

Putative uncharacterized protein; putative phospholipase (RT0522, Pat2)

CENTER ID: RityA.17851.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.17851.a.B1.PW37597 full length 1 584

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|257363.4.peg.547
RefSeq: YP_067473.1
UniProt: Q68WK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKQEFKTSVF NEQSEILEQL EYITKDQNSS INQIKTLYEL EKAQKAPIEY IAFSGGGAKG AIYSGVYEAA KKAGILDNVK AVAGSSVGAI TAAVVALGTP PKRFEEISKN TNLQTLFGKQ GFSAGMIQLN KDGTPLYDLM ELIIKENIGN FLHRSDIVNV KNDKALNELR ARYNQKDGKI YFKDIALLRK YDPVQYKDLV ITATQRETSA LTIFNSFDTP NVEIALACRA SASIPIVFKP VEIDGKKYVD GGYRDNIPTK YFKDNEPEFC IQEVTDNLEE ITLAKKQGRT LAMAFGTGMG ADANIAIYSA KNFDSPSDII KFLVDVLFKI IAQVGGKFKY TETLRATNEQ LRENSLNTVV LDTAGIDTLD FKDAQKYSDY LHIKGYCQTL EHLNNHDLTT KIDKTFDHQK FLLNVYEVYN NKNLHKTFGT KLFGMFIPSK DNNTSKWQDG IIETHNDKAK MLLSFCKIGL LTEEKLNEKL REYVIIAATS RNNTIKTDTN SLNALLYTLN TSVASSKIKD SFIEVLEIDK NKDTRFDKTK TFDYNIANFQ FTKKDLESFL AKKKSAALKI QHKYDLVRNH RNRW
NT Sequence
atgaagcaag agttcaaaac ttctgtattt aatgagcaat ctgaaatctt agagcagtta gaatacataa caaaagatca aaattcttct atcaatcaaa taaaaacatt gtatgagtta gaaaaagcac aaaaagcgcc tatcgaatat atagctttta gtggtggtgg agctaagggt gcaatatatt ccggagttta tgaggcagca aaaaaagctg gtattttaga taatgttaag gctgtagcag gttcttctgt tggtgctatc actgctgcag ttgtagctct tgggacaccg cctaagcgat ttgaagaaat ttctaaaaat acaaatctgc aaactttatt tgggaaacaa ggcttttctg caggtatgat acagttaaat aaagacggta cgcctttata tgatttaatg gaattgatta ttaaagaaaa tataggtaat tttttacata gatcagatat agtaaatgtt aaaaatgata aggctcttaa tgaattaaga gcaagatata accaaaagga tggtaaaata tattttaaag atatagcatt attacgaaaa tatgatcctg tacaatataa ggatttagta attacagcaa cacaacgaga gacaagtgca ttaactattt ttaatagttt tgatacacca aatgttgaaa ttgctttagc ttgtcgtgct tctgcttcta tacctatagt ttttaagccg gtagaaattg atggtaaaaa atatgttgat ggtggatata gggataatat accgacaaaa tattttaaag ataatgaacc agaattttgt atacaagaag taactgataa tttggaagaa attacattag ctaaaaagca aggtagaact ctggctatgg cttttggtac tggtatggga gctgatgcaa atattgctat atatagtgca aaaaattttg atagtccaag tgatataatc aagtttttag ttgatgtatt atttaaaatt attgcacaag taggaggaaa atttaaatat actgaaactt taagagcaac taatgagcaa ttacgtgaaa attctttaaa taccgttgtt ttagatactg ctggaataga tactttggat tttaaagatg cacaaaaata ttctgattat ttgcatatta aaggatattg tcaaactcta gaacatttga ataatcatga tcttactaca aaaatagata aaacatttga tcatcaaaaa tttctactaa atgtttatga agtttataat aataaaaatc tacataaaac atttggcact aaattatttg gaatgtttat tccatcaaaa gacaataata cttctaaatg gcaagacggc attattgaga ctcataacga taaagcaaaa atgctattat ctttttgtaa aatcggatta ttgaccgaag aaaaattaaa tgaaaaactg agagaatatg taattattgc tgcaactagt cgtaacaata caataaagac cgatacaaac tcattgaatg ctttattgta taccttaaat acttctgttg cttcaagtaa aataaaagat agtttcattg aggtgttaga aatagataaa aataaagata caaggtttga taaaactaaa acctttgatt ataatattgc taattttcaa tttaccaaaa aagatttaga aagtttttta gcaaagaaaa aaagcgctgc gctaaaaatt caacacaaat atgatttagt taggaatcac aggaataggt ggtaa
Details for RityA.17851.a.B1.PW37597
PURIFICATION DATe: 11/17/2014
CONCENTRATION: 18mg/ml
OBSERVED MW: 67kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK QEFKTSVFNE QSEILEQLEY ITKDQNSSIN QIKTLYELEK AQKAPIEYIA FSGGGAKGAI YSGVYEAAKK AGILDNVKAV AGSSVGAITA AVVALGTPPK RFEEISKNTN LQTLFGKQGF SAGMIQLNKD GTPLYDLMEL IIKENIGNFL HRSDIVNVKN DKALNELRAR YNQKDGKIYF KDIALLRKYD PVQYKDLVIT ATQRETSALT IFNSFDTPNV EIALACRASA SIPIVFKPVE IDGKKYVDGG YRDNIPTKYF KDNEPEFCIQ EVTDNLEEIT LAKKQGRTLA MAFGTGMGAD ANIAIYSAKN FDSPSDIIKF LVDVLFKIIA QVGGKFKYTE TLRATNEQLR ENSLNTVVLD TAGIDTLDFK DAQKYSDYLH IKGYCQTLEH LNNHDLTTKI DKTFDHQKFL LNVYEVYNNK NLHKTFGTKL FGMFIPSKDN NTSKWQDGII ETHNDKAKML LSFCKIGLLT EEKLNEKLRE YVIIAATSRN NTIKTDTNSL NALLYTLNTS VASSKIKDSF IEVLEIDKNK DTRFDKTKTF DYNIANFQFT KKDLESFLAK KKSAALKIQH KYDLVRNHRN RW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC AAGAGTTCAA AACTTCTGTA TTTAATGAGC AATCTGAAAT CTTAGAGCAG TTAGAATACA TAACAAAAGA TCAAAATTCT TCTATCAATC AAATAAAAAC ATTGTATGAG TTAGAAAAAG CACAAAAAGC GCCTATCGAA TATATAGCTT TTAGTGGTGG TGGAGCTAAG GGTGCAATAT ATTCCGGAGT TTATGAGGCA GCAAAAAAAG CTGGTATTTT AGATAATGTT AAGGCTGTAG CAGGTTCTTC TGTTGGTGCT ATCACTGCTG CAGTTGTAGC TCTTGGGACA CCGCCTAAGC GATTTGAAGA AATTTCTAAA AATACAAATC TGCAAACTTT ATTTGGGAAA CAAGGCTTTT CTGCAGGTAT GATACAGTTA AATAAAGACG GTACGCCTTT ATATGATTTA ATGGAATTGA TTATTAAAGA AAATATAGGT AATTTTTTAC ATAGATCAGA TATAGTAAAT GTTAAAAATG ATAAGGCTCT TAATGAATTA AGAGCAAGAT ATAACCAAAA GGATGGTAAA ATATATTTTA AAGATATAGC ATTATTACGA AAATATGATC CTGTACAATA TAAGGATTTA GTAATTACAG CAACACAACG AGAGACAAGT GCATTAACTA TTTTTAATAG TTTTGATACA CCAAATGTTG AAATTGCTTT AGCTTGTCGT GCTTCTGCTT CTATACCTAT AGTTTTTAAG CCGGTAGAAA TTGATGGTAA AAAATATGTT GATGGTGGAT ATAGGGATAA TATACCGACA AAATATTTTA AAGATAATGA ACCAGAATTT TGTATACAAG AAGTAACTGA TAATTTGGAA GAAATTACAT TAGCTAAAAA GCAAGGTAGA ACTCTGGCTA TGGCTTTTGG TACTGGTATG GGAGCTGATG CAAATATTGC TATATATAGT GCAAAAAATT TTGATAGTCC AAGTGATATA ATCAAGTTTT TAGTTGATGT ATTATTTAAA ATTATTGCAC AAGTAGGAGG AAAATTTAAA TATACTGAAA CTTTAAGAGC AACTAATGAG CAATTACGTG AAAATTCTTT AAATACCGTT GTTTTAGATA CTGCTGGAAT AGATACTTTG GATTTTAAAG ATGCACAAAA ATATTCTGAT TATTTGCATA TTAAAGGATA TTGTCAAACT CTAGAACATT TGAATAATCA TGATCTTACT ACAAAAATAG ATAAAACATT TGATCATCAA AAATTTCTAC TAAATGTTTA TGAAGTTTAT AATAATAAAA ATCTACATAA AACATTTGGC ACTAAATTAT TTGGAATGTT TATTCCATCA AAAGACAATA ATACTTCTAA ATGGCAAGAC GGCATTATTG AGACTCATAA CGATAAAGCA AAAATGCTAT TATCTTTTTG TAAAATCGGA TTATTGACCG AAGAAAAATT AAATGAAAAA CTGAGAGAAT ATGTAATTAT TGCTGCAACT AGTCGTAACA ATACAATAAA GACCGATACA AACTCATTGA ATGCTTTATT GTATACCTTA AATACTTCTG TTGCTTCAAG TAAAATAAAA GATAGTTTCA TTGAGGTGTT AGAAATAGAT AAAAATAAAG ATACAAGGTT TGATAAAACT AAAACCTTTG ATTATAATAT TGCTAATTTT CAATTTACCA AAAAAGATTT AGAAAGTTTT TTAGCAAAGA AAAAAAGCGC TGCGCTAAAA ATTCAACACA AATATGATTT AGTTAGGAAT CACAGGAATA GGTGGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT