RityA.17834.a

Putative uncharacterized protein

CENTER ID: RityA.17834.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.17834.a.B1.GE35398 Full length( RityA.17834.a ) 1 551
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.17834.a.B1.PS01612 Full length( RityA.17834.a ) 1 551

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|257363.4.peg.795
RefSeq: YP_067714.1
UniProt: Q68VW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLKSSTTND HDKTTKINSI QSLVNCTDTV ADHNTYDEIP YESYPYAITN PYHLSTLATL FGINAPEVEN SKILELGCAA GGNLIPHAVL YPKAHFVGVD LSKVQIDEAN KNVRALGLKN IEFHHCSITD INDSFGKFDY IICHGVISWV PKIVRDKIFK VCNSNLSTNG IAYISYNTLP GWNMVRTIRD MMLYHSSSFT NVRDRIAQSR LLLEFVKDSL ENSKTPYAEV LKTEAGLLAK QTDHYLRHDH LEEENAQFYF HEFMNEARKY NLQYLADCNI STMYLGNMPP KVVEQLKAVN DIVRTEQYMD FITNRRFRTT LLCHNDLKIN RNINNEDIKK FNIIFNVVPE KPLQEVDLDN AAENLQFFLN GNKECNLSTT SPYMKAILYT FSENLNNPLS FKQVTSEANK KLNNNKLNEI KNELLNNAMK LVLQGYISIT NQKHRNKPVL DKPKTTQMVL YQAKHTPSMW VTNLKHEPIG VNFFEKFALR YMDGKNDKKA IIEAILGHVE KGELTLSKEG QKIENKEEIR KELESLFTPM IEKFASNALL V
NT Sequence
ATGTCACTAA AATCTAGTAC TACTAACGAT CATGATAAAA CAACAAAAAT AAATTCTATA CAATCCTTGG TGAACTGTAC TGATACGGTT GCAGATCATA ACACTTATGA TGAAATACCA TATGAAAGCT ACCCATATGC TATTACCAAT CCTTATCACT TAAGTACACT TGCAACTCTT TTCGGTATCA ATGCTCCTGA AGTTGAAAAT TCAAAAATAT TAGAGCTTGG TTGTGCGGCA GGAGGTAATT TAATACCACA TGCAGTTCTT TATCCAAAAG CTCATTTTGT TGGAGTCGAT TTATCTAAGG TACAAATTGA TGAAGCAAAT AAAAATGTTA GAGCGTTAGG ACTCAAAAAT ATAGAATTTC ATCACTGTTC GATAACAGAT ATTAACGATT CTTTCGGTAA ATTTGATTAT ATAATTTGCC ATGGTGTAAT TTCTTGGGTG CCAAAAATCG TTAGAGATAA AATTTTTAAA GTGTGTAATA GTAATCTTAG CACGAATGGA ATAGCATATA TTAGCTATAA TACGCTACCT GGTTGGAATA TGGTGCGCAC TATCCGAGAT ATGATGCTTT ATCATTCTAG TTCATTTACA AATGTGCGTG ATAGGATTGC TCAATCTAGA TTGTTGTTAG AATTTGTTAA GGATAGCTTA GAGAACTCTA AAACACCTTA TGCAGAAGTA TTAAAAACTG AGGCAGGTCT ACTTGCTAAA CAAACTGATC ATTATTTACG TCATGATCAT CTAGAAGAAG AAAATGCTCA GTTCTATTTT CATGAATTTA TGAATGAGGC AAGAAAGTAT AATTTACAAT ATTTAGCTGA TTGTAATATA TCAACTATGT ATCTTGGTAA TATGCCGCCA AAAGTAGTAG AGCAGTTAAA AGCAGTAAAT GATATAGTTA GAACTGAACA ATATATGGAT TTTATTACGA ATCGTCGTTT TAGAACAACT TTACTATGTC ATAATGATCT AAAAATTAAT AGAAATATTA ATAATGAGGA TATAAAAAAA TTTAATATAA TCTTTAATGT AGTTCCTGAA AAACCACTTC AAGAAGTAGA TCTTGATAAT GCTGCCGAAA ATTTACAATT CTTCTTAAAC GGTAATAAAG AGTGTAATTT ATCAACAACT TCACCTTATA TGAAGGCTAT TTTATACACT TTTAGTGAGA ATCTAAATAA TCCATTAAGT TTTAAGCAAG TAACGTCTGA AGCTAATAAA AAACTAAATA ATAATAAATT AAACGAAATC AAAAATGAGC TTTTAAATAA CGCAATGAAG CTAGTACTCC AAGGTTATAT TAGTATTACA AATCAGAAGC ATAGGAATAA ACCAGTGCTT GATAAGCCTA AAACAACACA AATGGTGCTA TATCAAGCAA AACATACACC TTCTATGTGG GTTACAAATT TAAAACATGA ACCAATAGGC GTTAATTTCT TTGAGAAGTT TGCACTTAGA TATATGGATG GTAAAAATGA TAAAAAAGCA ATAATTGAAG CTATACTTGG TCATGTAGAA AAAGGTGAAT TAACTTTAAG TAAAGAAGGG CAAAAAATAG AAAATAAAGA AGAAATACGC AAAGAACTAG AAAGCTTATT TACGCCAATG ATCGAGAAAT TTGCTTCTAA TGCGTTATTA GTA
Details for RityA.17834.a.B1.GE35398
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 64
Validated AA Sequence
MVRTIRDMML YHSSSFTNVR DRIAQSRLLL EFVKDSLENS KTPYAEVLKT EAGLLAKQTD HYLRHDHLEE ENAQFYFHEF MNEARKYNLQ YLADCNISTM YLGNMPPKVV EQLKAVNDIV RTEQYMXXIT NRRFRTTLLC HNDLKXXRNI NXEDIKKXNX IFNVVPEKPL QEVDLDNAAE NLQFFLNGNK ECNLSTTSPY MKAILYTFSE NLNNPLSFKQ VTSEANKKLN NNKLNEIKNE LLNNAMKLVL QGYISITNQK HRNKPVLDKP KTTQMVLYQA KHTPSMWVTN LKHEPIGVNF FEKFALRYMD GKNDKKAIIE AILGHVEKGE LTLSKEGQKI ENKEEIRKEL ESLFTPMIE
Validated NT Sequence
tctcgatcat tggcgtaaat aagctttcta gttctttgcg tatttcttct ttattttcta ttttttgccc ttctttactt aaagttaatt cacctttttc tacatgacca agtatagctt caattattgc ttttttatca tttttaccat ccatatatct aagtgcaaac ttctcaaaga aattaacgcc tattggttca tgttttaaat ttgtaaccca catagaaggt gtatgttttg cttgatatag caccatttgt gttgttttag gcttatcaag cactggttta ttcctatgct tctgatttgt aatactaata taaccttgga gtactagctt cattgcgtta tttaaaagct catttttgat ttcgtttaat ttattattat ttagtttttt attagcttca gacgttactt gcttaaaact taatggatta tttagattct cactaaaagt gtataaaata gccttcatat aaggtgaagt tgttgataaa ttacactctt tattaccgtt taagaagaat tgtaaatttt cggcagcatt atcaagatct acttcttgaa gtggtttttc aggaactaca ttaaagatna tattannttt ttttatatcc tcnttattaa tatttctatn nnnttttaga tcattatgac atagtaaagt tgttctaaaa cgacgattcg taatnaannc catatattgt tcagttctaa ctatatcatt tactgctttt aactgctcta ctacttttgg cggcatatta ccaagataca tagttgatat attacaatca gctaaatatt gtaaattata ctttcttgcc tcattcataa attcatgaaa atagaactga gcattttctt cttctagatg atcatgacgt aaataatgat cagtttgttt agcaagtaga cctgcctcag tttttaatac ttctgcataa ggtgttttag agttctctaa gctatcctta acaaattcta acaacaatct agattgagca atcctatcac gcacatttgt aaatgaacta gaatgataaa gcatcatatc tcggatagtg cgcaccatat tccaaccagg tagcgtatta tagctaatat atgctattcc attcgtgcta agattactat tacacacttt aaaaatttta tctctaacga tttttggcac ccaagaaatt acaccatggc aaattatata atcaaattta ccgaaagaat cgttaatatc tgttatcgaa cagtgatgaa attctatatt tttgagtcct aacgctctaa catttttatt tgcttcatca atttgtacct tagataaatc gactccaaca aaatgagctt ttggataaag aactgcatgt ggtattaaat tacctcctgc cgcacaacca agctctaata tttttgaatt ttcaacttca ggagcattga taccgaaaag agttgcaagt gtacttaagt gataaggatt ggtaatagca tatgggtagc tttcatatgg tatttcatca taagtgttat gatctgcaac cgtatcagta cagttcacca aggattgtat agaatttatt tttgttgttt tatcatgatc gttagtagta ctagatttta g
Expected Protein Sequence
MAHHHHHHMS LKSSTTNDHD KTTKINSIQS LVNCTDTVAD HNTYDEIPYE SYPYAITNPY HLSTLATLFG INAPEVENSK ILELGCAAGG NLIPHAVLYP KAHFVGVDLS KVQIDEANKN VRALGLKNIE FHHCSITDIN DSFGKFDYII CHGVISWVPK IVRDKIFKVC NSNLSTNGIA YISYNTLPGW NMVRTIRDMM LYHSSSFTNV RDRIAQSRLL LEFVKDSLEN SKTPYAEVLK TEAGLLAKQT DHYLRHDHLE EENAQFYFHE FMNEARKYNL QYLADCNIST MYLGNMPPKV VEQLKAVNDI VRTEQYMDFI TNRRFRTTLL CHNDLKINRN INNEDIKKFN IIFNVVPEKP LQEVDLDNAA ENLQFFLNGN KECNLSTTSP YMKAILYTFS ENLNNPLSFK QVTSEANKKL NNNKLNEIKN ELLNNAMKLV LQGYISITNQ KHRNKPVLDK PKTTQMVLYQ AKHTPSMWVT NLKHEPIGVN FFEKFALRYM DGKNDKKAII EAILGHVEKG ELTLSKEGQK IENKEEIRKE LESLFTPMIE KFASNALLV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcac taaaatctag tactactaac gatcatgata aaacaacaaa aataaattct atacaatcct tggtgaactg tactgatacg gttgcagatc ataacactta tgatgaaata ccatatgaaa gctacccata tgctattacc aatccttatc acttaagtac acttgcaact cttttcggta tcaatgctcc tgaagttgaa aattcaaaaa tattagagct tggttgtgcg gcaggaggta atttaatacc acatgcagtt ctttatccaa aagctcattt tgttggagtc gatttatcta aggtacaaat tgatgaagca aataaaaatg ttagagcgtt aggactcaaa aatatagaat ttcatcactg ttcgataaca gatattaacg attctttcgg taaatttgat tatataattt gccatggtgt aatttcttgg gtgccaaaaa tcgttagaga taaaattttt aaagtgtgta atagtaatct tagcacgaat ggaatagcat atattagcta taatacgcta cctggttgga atatggtgcg cactatccga gatatgatgc tttatcattc tagttcattt acaaatgtgc gtgataggat tgctcaatct agattgttgt tagaatttgt taaggatagc ttagagaact ctaaaacacc ttatgcagaa gtattaaaaa ctgaggcagg tctacttgct aaacaaactg atcattattt acgtcatgat catctagaag aagaaaatgc tcagttctat tttcatgaat ttatgaatga ggcaagaaag tataatttac aatatttagc tgattgtaat atatcaacta tgtatcttgg taatatgccg ccaaaagtag tagagcagtt aaaagcagta aatgatatag ttagaactga acaatatatg gattttatta cgaatcgtcg ttttagaaca actttactat gtcataatga tctaaaaatt aatagaaata ttaataatga ggatataaaa aaatttaata taatctttaa tgtagttcct gaaaaaccac ttcaagaagt agatcttgat aatgctgccg aaaatttaca attcttctta aacggtaata aagagtgtaa tttatcaaca acttcacctt atatgaaggc tattttatac acttttagtg agaatctaaa taatccatta agttttaagc aagtaacgtc tgaagctaat aaaaaactaa ataataataa attaaacgaa atcaaaaatg agcttttaaa taacgcaatg aagctagtac tccaaggtta tattagtatt acaaatcaga agcataggaa taaaccagtg cttgataagc ctaaaacaac acaaatggtg ctatatcaag caaaacatac accttctatg tgggttacaa atttaaaaca tgaaccaata ggcgttaatt tctttgagaa gtttgcactt agatatatgg atggtaaaaa tgataaaaaa gcaataattg aagctatact tggtcatgta gaaaaaggtg aattaacttt aagtaaagaa gggcaaaaaa tagaaaataa agaagaaata cgcaaagaac tagaaagctt atttacgcca atgatcgaga aatttgcttc taatgcgtta ttagtatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for RityA.17834.a.B1.PS01612
PURIFICATION DATe: 8/28/2012
CONCENTRATION: 66.8mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 64
Protocol Notes
notes unavailable
Validated AA Sequence
MVRTIRDMML YHSSSFTNVR DRIAQSRLLL EFVKDSLENS KTPYAEVLKT EAGLLAKQTD HYLRHDHLEE ENAQFYFHEF MNEARKYNLQ YLADCNISTM YLGNMPPKVV EQLKAVNDIV RTEQYMXXIT NRRFRTTLLC HNDLKXXRNI NXEDIKKXNX IFNVVPEKPL QEVDLDNAAE NLQFFLNGNK ECNLSTTSPY MKAILYTFSE NLNNPLSFKQ VTSEANKKLN NNKLNEIKNE LLNNAMKLVL QGYISITNQK HRNKPVLDKP KTTQMVLYQA KHTPSMWVTN LKHEPIGVNF FEKFALRYMD GKNDKKAIIE AILGHVEKGE LTLSKEGQKI ENKEEIRKEL ESLFTPMIE
Validated NT Sequence
tctcgatcat tggcgtaaat aagctttcta gttctttgcg tatttcttct ttattttcta ttttttgccc ttctttactt aaagttaatt cacctttttc tacatgacca agtatagctt caattattgc ttttttatca tttttaccat ccatatatct aagtgcaaac ttctcaaaga aattaacgcc tattggttca tgttttaaat ttgtaaccca catagaaggt gtatgttttg cttgatatag caccatttgt gttgttttag gcttatcaag cactggttta ttcctatgct tctgatttgt aatactaata taaccttgga gtactagctt cattgcgtta tttaaaagct catttttgat ttcgtttaat ttattattat ttagtttttt attagcttca gacgttactt gcttaaaact taatggatta tttagattct cactaaaagt gtataaaata gccttcatat aaggtgaagt tgttgataaa ttacactctt tattaccgtt taagaagaat tgtaaatttt cggcagcatt atcaagatct acttcttgaa gtggtttttc aggaactaca ttaaagatna tattannttt ttttatatcc tcnttattaa tatttctatn nnnttttaga tcattatgac atagtaaagt tgttctaaaa cgacgattcg taatnaannc catatattgt tcagttctaa ctatatcatt tactgctttt aactgctcta ctacttttgg cggcatatta ccaagataca tagttgatat attacaatca gctaaatatt gtaaattata ctttcttgcc tcattcataa attcatgaaa atagaactga gcattttctt cttctagatg atcatgacgt aaataatgat cagtttgttt agcaagtaga cctgcctcag tttttaatac ttctgcataa ggtgttttag agttctctaa gctatcctta acaaattcta acaacaatct agattgagca atcctatcac gcacatttgt aaatgaacta gaatgataaa gcatcatatc tcggatagtg cgcaccatat tccaaccagg tagcgtatta tagctaatat atgctattcc attcgtgcta agattactat tacacacttt aaaaatttta tctctaacga tttttggcac ccaagaaatt acaccatggc aaattatata atcaaattta ccgaaagaat cgttaatatc tgttatcgaa cagtgatgaa attctatatt tttgagtcct aacgctctaa catttttatt tgcttcatca atttgtacct tagataaatc gactccaaca aaatgagctt ttggataaag aactgcatgt ggtattaaat tacctcctgc cgcacaacca agctctaata tttttgaatt ttcaacttca ggagcattga taccgaaaag agttgcaagt gtacttaagt gataaggatt ggtaatagca tatgggtagc tttcatatgg tatttcatca taagtgttat gatctgcaac cgtatcagta cagttcacca aggattgtat agaatttatt tttgttgttt tatcatgatc gttagtagta ctagatttta g
Expressed Protein Sequence
MAHHHHHHMS LKSSTTNDHD KTTKINSIQS LVNCTDTVAD HNTYDEIPYE SYPYAITNPY HLSTLATLFG INAPEVENSK ILELGCAAGG NLIPHAVLYP KAHFVGVDLS KVQIDEANKN VRALGLKNIE FHHCSITDIN DSFGKFDYII CHGVISWVPK IVRDKIFKVC NSNLSTNGIA YISYNTLPGW NMVRTIRDMM LYHSSSFTNV RDRIAQSRLL LEFVKDSLEN SKTPYAEVLK TEAGLLAKQT DHYLRHDHLE EENAQFYFHE FMNEARKYNL QYLADCNIST MYLGNMPPKV VEQLKAVNDI VRTEQYMDFI TNRRFRTTLL CHNDLKINRN INNEDIKKFN IIFNVVPEKP LQEVDLDNAA ENLQFFLNGN KECNLSTTSP YMKAILYTFS ENLNNPLSFK QVTSEANKKL NNNKLNEIKN ELLNNAMKLV LQGYISITNQ KHRNKPVLDK PKTTQMVLYQ AKHTPSMWVT NLKHEPIGVN FFEKFALRYM DGKNDKKAII EAILGHVEKG ELTLSKEGQK IENKEEIRKE LESLFTPMIE KFASNALLV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAC TAAAATCTAG TACTACTAAC GATCATGATA AAACAACAAA AATAAATTCT ATACAATCCT TGGTGAACTG TACTGATACG GTTGCAGATC ATAACACTTA TGATGAAATA CCATATGAAA GCTACCCATA TGCTATTACC AATCCTTATC ACTTAAGTAC ACTTGCAACT CTTTTCGGTA TCAATGCTCC TGAAGTTGAA AATTCAAAAA TATTAGAGCT TGGTTGTGCG GCAGGAGGTA ATTTAATACC ACATGCAGTT CTTTATCCAA AAGCTCATTT TGTTGGAGTC GATTTATCTA AGGTACAAAT TGATGAAGCA AATAAAAATG TTAGAGCGTT AGGACTCAAA AATATAGAAT TTCATCACTG TTCGATAACA GATATTAACG ATTCTTTCGG TAAATTTGAT TATATAATTT GCCATGGTGT AATTTCTTGG GTGCCAAAAA TCGTTAGAGA TAAAATTTTT AAAGTGTGTA ATAGTAATCT TAGCACGAAT GGAATAGCAT ATATTAGCTA TAATACGCTA CCTGGTTGGA ATATGGTGCG CACTATCCGA GATATGATGC TTTATCATTC TAGTTCATTT ACAAATGTGC GTGATAGGAT TGCTCAATCT AGATTGTTGT TAGAATTTGT TAAGGATAGC TTAGAGAACT CTAAAACACC TTATGCAGAA GTATTAAAAA CTGAGGCAGG TCTACTTGCT AAACAAACTG ATCATTATTT ACGTCATGAT CATCTAGAAG AAGAAAATGC TCAGTTCTAT TTTCATGAAT TTATGAATGA GGCAAGAAAG TATAATTTAC AATATTTAGC TGATTGTAAT ATATCAACTA TGTATCTTGG TAATATGCCG CCAAAAGTAG TAGAGCAGTT AAAAGCAGTA AATGATATAG TTAGAACTGA ACAATATATG GATTTTATTA CGAATCGTCG TTTTAGAACA ACTTTACTAT GTCATAATGA TCTAAAAATT AATAGAAATA TTAATAATGA GGATATAAAA AAATTTAATA TAATCTTTAA TGTAGTTCCT GAAAAACCAC TTCAAGAAGT AGATCTTGAT AATGCTGCCG AAAATTTACA ATTCTTCTTA AACGGTAATA AAGAGTGTAA TTTATCAACA ACTTCACCTT ATATGAAGGC TATTTTATAC ACTTTTAGTG AGAATCTAAA TAATCCATTA AGTTTTAAGC AAGTAACGTC TGAAGCTAAT AAAAAACTAA ATAATAATAA ATTAAACGAA ATCAAAAATG AGCTTTTAAA TAACGCAATG AAGCTAGTAC TCCAAGGTTA TATTAGTATT ACAAATCAGA AGCATAGGAA TAAACCAGTG CTTGATAAGC CTAAAACAAC ACAAATGGTG CTATATCAAG CAAAACATAC ACCTTCTATG TGGGTTACAA ATTTAAAACA TGAACCAATA GGCGTTAATT TCTTTGAGAA GTTTGCACTT AGATATATGG ATGGTAAAAA TGATAAAAAA GCAATAATTG AAGCTATACT TGGTCATGTA GAAAAAGGTG AATTAACTTT AAGTAAAGAA GGGCAAAAAA TAGAAAATAA AGAAGAAATA CGCAAAGAAC TAGAAAGCTT ATTTACGCCA ATGATCGAGA AATTTGCTTC TAATGCGTTA TTAGTATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T