RityA.17583.b
Post-proline cleaving enzyme
| CENTER ID: | RityA.17583.b |
| ORGANISM: | Rickettsia typhi str. Wilmington |
| ASSOCIATED DISEASE: | Murine typhus (endemic typhus) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RityA.17583.b.B2.GE35384 | Mature protein( RityA.17583.b ) | 19 | 720 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4HVT
DEPOSITED: 11/7/2012
DETERMINATION: XRay
CLONE: RityA.17583.b.B2.GE35384
PROTEIN: RityA.17583.b.B2.PS01616
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_156367 |
| PATRIC ID: | fig|257363.4.peg.171 |
| RefSeq: | YP_067131.1 |
| UniProt: | Q68XJ3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKIICYLTI ILLTFCIDAM EDNNKQIFNP KETKFLEEAE GVEALEWAKE RTSKTEKALQ AMQEYKQIKK EIETIFYDQR KTPYGVIRKG YVYNFWMDDK NPQGLWRRTL VDNYSKDKPN WEVLIDFDKL SKKIGKKVAY RGVSNCFQNP NRYLISMSFG GKDEMFFREW DLEKKDFVKN GFEPITNSGK LLEGKFTYPT WINKDTIIFN LVLHKNEITS SLYPNSLYIW KRGESIEKAK KLFEVPKEYI YVSAGKLLSD TISSSLIFIS ANKDFYNYDN YILDTKYKNL KLQKINMPSD ATLQGSFKEY VFWLLRSDWK FKSHNIKAGS LVALHFTDLL KTESDKTSLK ILFTPTANEV FNFISTTKDR VFLATYDNVV AKVVTFTLEN EQWTKPVVLK LPYQNAIFGM SSYEEEEEAL ITIENSIVPP TIYLWVKTHE LKIIRKALYS FDSENYVLEQ KEATSFDGVK IPYFLVYKKG IKFDGKNPTL LEAYGGFQVI NAPYFSRIKN EVWVKNAGVS VLANIRGGGE FGPEWHKSAQ GIKRQTAFND FFAVSEELIK QNITSPEYLG IKGGSNGGLL VSVAMTQRPE LFGAVACEVP ILDMIRYKEF GAGHSWVTEY GDPEIPNDLL HIKKYAPLEN LSLTQKYPTV LITDSVLDQR VHPWHGRIFE YVLAQNPNTK TYFLESKDSG HGSGSDLKES ANYFINLYTF FANALKLKIN
NT Sequence
ATGATAAAAA TTATATGCTA TTTGACAATT ATTTTATTAA CCTTTTGTAT TGATGCTATG GAAGATAATA ATAAACAAAT ATTTAACCCA AAAGAGACAA AATTTTTAGA AGAAGCTGAA GGAGTGGAAG CGCTAGAATG GGCAAAAGAA CGTACTAGTA AAACAGAAAA AGCTTTGCAA GCAATGCAGG AATATAAACA GATCAAAAAA GAAATAGAAA CTATTTTCTA TGACCAAAGA AAAACTCCAT ATGGTGTTAT ACGTAAAGGT TATGTGTATA ATTTTTGGAT GGATGATAAA AATCCGCAAG GATTATGGCG TAGAACGTTA GTTGATAATT ACTCTAAAGA TAAACCAAAT TGGGAAGTGC TAATCGATTT TGATAAATTA TCAAAAAAAA TCGGTAAAAA AGTAGCCTAT CGAGGTGTAA GTAATTGTTT CCAAAACCCT AATCGTTACT TAATTTCTAT GTCCTTTGGT GGTAAAGATG AGATGTTTTT TAGAGAATGG GATTTAGAAA AAAAAGATTT TGTTAAAAAT GGCTTTGAAC CGATAACAAA TAGCGGGAAA TTATTAGAAG GAAAATTTAC TTATCCTACT TGGATCAACA AAGATACTAT TATATTTAAT CTAGTTTTGC ATAAAAATGA AATAACGTCC TCTTTATATC CTAACTCGCT ATACATATGG AAGCGCGGAG AATCTATAGA AAAAGCTAAA AAACTTTTTG AAGTACCAAA AGAATATATA TATGTATCAG CTGGTAAACT TTTATCAGAT ACTATTTCTT CATCTTTAAT ATTTATATCT GCAAATAAAG ATTTTTATAA TTATGACAAT TATATTTTAG ATACTAAATA TAAGAACTTA AAATTACAAA AAATAAACAT GCCATCAGAT GCAACACTTC AAGGTTCTTT TAAAGAATAT GTATTTTGGT TGCTGCGTTC TGATTGGAAA TTTAAAAGTC ATAATATAAA GGCAGGCTCG CTGGTTGCAT TACACTTTAC AGACCTACTA AAAACAGAAA GTGATAAAAC TAGCCTAAAA ATATTATTTA CACCAACAGC GAATGAAGTA TTTAATTTCA TAAGCACAAC TAAAGATAGA GTATTTTTAG CAACTTACGA TAATGTGGTT GCAAAAGTTG TAACATTTAC TTTAGAAAAT GAACAATGGA CAAAACCGGT AGTCTTAAAG CTACCTTATC AGAACGCAAT TTTTGGGATG TCTTCTTATG AAGAGGAAGA AGAGGCATTA ATTACTATAG AAAATTCGAT AGTTCCACCA ACTATCTATT TATGGGTTAA AACTCATGAG TTAAAAATTA TTCGAAAAGC TTTATACTCT TTTGATAGTG AAAATTATGT TTTAGAACAA AAGGAAGCTA CAAGCTTTGA TGGAGTTAAA ATACCATATT TTCTTGTTTA TAAGAAAGGG ATAAAATTTG ACGGTAAAAA TCCAACATTA CTTGAAGCAT ATGGTGGATT CCAGGTAATC AATGCACCAT ATTTTTCACG GATTAAAAAT GAAGTATGGG TTAAAAATGC TGGTGTTAGT GTTCTTGCTA ATATCAGAGG TGGTGGAGAG TTTGGTCCTG AGTGGCATAA GTCAGCTCAA GGAATAAAAC GTCAAACAGC CTTTAATGAT TTTTTTGCAG TATCAGAGGA GTTAATAAAG CAAAATATTA CTAGCCCTGA ATATTTAGGA ATTAAAGGAG GGAGTAACGG CGGATTACTA GTCAGTGTTG CAATGACACA ACGTCCTGAG TTATTTGGTG CTGTAGCATG CGAAGTACCG ATACTTGATA TGATACGTTA TAAAGAATTT GGAGCAGGAC ATTCATGGGT AACTGAATAT GGTGATCCAG AAATTCCAAA TGATTTATTA CATATTAAAA AATATGCACC GTTAGAGAAT TTATCTTTAA CACAAAAATA CCCAACTGTG TTAATTACAG ATTCAGTATT GGATCAGCGT GTACACCCAT GGCATGGAAG AATTTTTGAG TATGTACTTG CACAAAATCC AAATACTAAA ACTTACTTTC TAGAAAGTAA AGATAGTGGA CATGGTAGTG GTAGTGATTT AAAAGAGAGT GCAAATTATT TTATTAATCT CTATACATTT TTTGCAAATG CTTTAAAATT GAAAATTAAT
Details for RityA.17583.b.B2.GE35384
| HARVESTED ON: | 5/16/2012 |
| SEQUENCED ON: | 5/15/2012 |
| EXPECTED MW: | 82kDa |
| OBSERVED MW: | 82kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 47 |
Validated AA Sequence
MAHHHHHHMA MEDNNKQIFN PKETKFLEEA EGVEALEWAK ERTSKTEKAL QAMQEYKQIK KEIETIFYDQ RKTPYGVIRK GYVYNFWMDD KNPQGLWRRT LVDNYSKDKP NWEVLIDFDK LSKKIGKKVA YRGVSNCFQN PNRYLISMSF GGKDEMFFRE WDLEKKDFVK NGFEPITNSG KLLEGKFTYP TWINKDTIIF NLVLHKNEIT SSLYPNSLYI WKRGESIEKA KKLFEVPKEY IYVSAGKLLS DTISSSLIFI SANKDFYNYD NYILDTKYKN LKLQKINMPS DATLXGSFKE YVFWLLRSDW XFKSHNXKAG SLVAXXFTDL LKXESXKLA
Validated NT Sequence
ttgtnnnntt taagaaggag atataccatg gctcaccacc accaccacca tatggctatg gaagataata ataaacaaat atttaaccca aaagagacaa aatttttaga agaagctgaa ggagtggaag cgctagaatg ggcaaaagaa cgtactagta aaacagaaaa agctttgcaa gcaatgcagg aatataaaca gatcaaaaaa gaaatagaaa ctattttcta tgaccaaaga aaaactccat atggtgttat acgtaaaggt tatgtgtata atttttggat ggatgataaa aatccgcaag gattatggcg tagaacgtta gttgataatt actctaaaga taaaccaaat tgggaagtgc taatcgattt tgataaatta tcaaaaaaaa tcggtaaaaa agtagcctat cgaggtgtaa gtaattgttt ccaaaaccct aatcgttact taatttctat gtcctttggt ggtaaagatg agatgttttt tagagaatgg gatttagaaa aaaaagattt tgttaaaaat ggctttgaac cgataacaaa tagcgggaaa ttattagaag gaaaatttac ttatcctact tggatcaaca aagatactat tatatttaat ctagttttgc ataaaaatga aataacgtcc tctttatatc ctaactcgct atacatatgg aagcgcggag aatctataga aaaagctaaa aaactttttg aagtaccaaa agaatatata tatgtatcag ctggtaaact tttatcagat actatttctt catctttaat atttatatct gcaaataaag atttttataa ttatgacaat tatattttag atactaaata taagaactta aaattacaaa aaataaacat gccatcagat gcaacacttc naggntcttt taaagaatat gtattttggt tgctgcgttc tgattggnaa tttaaaagtc ataatntaaa ggcaggctcg ctggttgcat tnnnctttac agacctacta aaannngaaa gtganaaact agcctaaaaa tattnnttac nctnnntncn nnannnnnnn nnnnnnannn nttttngcan ntacganang nncaannnnn annntnnnnt nnnnannnnn atnnnnncnn nnnnnnnnnn nnnnnnnntn nnnnnnnnna nna
Expected Protein Sequence
MAHHHHHHMA MEDNNKQIFN PKETKFLEEA EGVEALEWAK ERTSKTEKAL QAMQEYKQIK KEIETIFYDQ RKTPYGVIRK GYVYNFWMDD KNPQGLWRRT LVDNYSKDKP NWEVLIDFDK LSKKIGKKVA YRGVSNCFQN PNRYLISMSF GGKDEMFFRE WDLEKKDFVK NGFEPITNSG KLLEGKFTYP TWINKDTIIF NLVLHKNEIT SSLYPNSLYI WKRGESIEKA KKLFEVPKEY IYVSAGKLLS DTISSSLIFI SANKDFYNYD NYILDTKYKN LKLQKINMPS DATLQGSFKE YVFWLLRSDW KFKSHNIKAG SLVALHFTDL LKTESDKTSL KILFTPTANE VFNFISTTKD RVFLATYDNV VAKVVTFTLE NEQWTKPVVL KLPYQNAIFG MSSYEEEEEA LITIENSIVP PTIYLWVKTH ELKIIRKALY SFDSENYVLE QKEATSFDGV KIPYFLVYKK GIKFDGKNPT LLEAYGGFQV INAPYFSRIK NEVWVKNAGV SVLANIRGGG EFGPEWHKSA QGIKRQTAFN DFFAVSEELI KQNITSPEYL GIKGGSNGGL LVSVAMTQRP ELFGAVACEV PILDMIRYKE FGAGHSWVTE YGDPEIPNDL LHIKKYAPLE NLSLTQKYPT VLITDSVLDQ RVHPWHGRIF EYVLAQNPNT KTYFLESKDS GHGSGSDLKE SANYFINLYT FFANALKLKI N
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta tggaagataa taataaacaa atatttaacc caaaagagac aaaattttta gaagaagctg aaggagtgga agcgctagaa tgggcaaaag aacgtactag taaaacagaa aaagctttgc aagcaatgca ggaatataaa cagatcaaaa aagaaataga aactattttc tatgaccaaa gaaaaactcc atatggtgtt atacgtaaag gttatgtgta taatttttgg atggatgata aaaatccgca aggattatgg cgtagaacgt tagttgataa ttactctaaa gataaaccaa attgggaagt gctaatcgat tttgataaat tatcaaaaaa aatcggtaaa aaagtagcct atcgaggtgt aagtaattgt ttccaaaacc ctaatcgtta cttaatttct atgtcctttg gtggtaaaga tgagatgttt tttagagaat gggatttaga aaaaaaagat tttgttaaaa atggctttga accgataaca aatagcggga aattattaga aggaaaattt acttatccta cttggatcaa caaagatact attatattta atctagtttt gcataaaaat gaaataacgt cctctttata tcctaactcg ctatacatat ggaagcgcgg agaatctata gaaaaagcta aaaaactttt tgaagtacca aaagaatata tatatgtatc agctggtaaa cttttatcag atactatttc ttcatcttta atatttatat ctgcaaataa agatttttat aattatgaca attatatttt agatactaaa tataagaact taaaattaca aaaaataaac atgccatcag atgcaacact tcaaggttct tttaaagaat atgtattttg gttgctgcgt tctgattgga aatttaaaag tcataatata aaggcaggct cgctggttgc attacacttt acagacctac taaaaacaga aagtgataaa actagcctaa aaatattatt tacaccaaca gcgaatgaag tatttaattt cataagcaca actaaagata gagtattttt agcaacttac gataatgtgg ttgcaaaagt tgtaacattt actttagaaa atgaacaatg gacaaaaccg gtagtcttaa agctacctta tcagaacgca atttttggga tgtcttctta tgaagaggaa gaagaggcat taattactat agaaaattcg atagttccac caactatcta tttatgggtt aaaactcatg agttaaaaat tattcgaaaa gctttatact cttttgatag tgaaaattat gttttagaac aaaaggaagc tacaagcttt gatggagtta aaataccata ttttcttgtt tataagaaag ggataaaatt tgacggtaaa aatccaacat tacttgaagc atatggtgga ttccaggtaa tcaatgcacc atatttttca cggattaaaa atgaagtatg ggttaaaaat gctggtgtta gtgttcttgc taatatcaga ggtggtggag agtttggtcc tgagtggcat aagtcagctc aaggaataaa acgtcaaaca gcctttaatg atttttttgc agtatcagag gagttaataa agcaaaatat tactagccct gaatatttag gaattaaagg agggagtaac ggcggattac tagtcagtgt tgcaatgaca caacgtcctg agttatttgg tgctgtagca tgcgaagtac cgatacttga tatgatacgt tataaagaat ttggagcagg acattcatgg gtaactgaat atggtgatcc agaaattcca aatgatttat tacatattaa aaaatatgca ccgttagaga atttatcttt aacacaaaaa tacccaactg tgttaattac agattcagta ttggatcagc gtgtacaccc atggcatgga agaatttttg agtatgtact tgcacaaaat ccaaatacta aaacttactt tctagaaagt aaagatagtg gacatggtag tggtagtgat ttaaaagaga gtgcaaatta ttttattaat ctctatacat tttttgcaaa tgctttaaaa ttgaaaatta attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat