RityA.17523.a
Putative uncharacterized protein
| CENTER ID: | RityA.17523.a |
| ORGANISM: | Rickettsia typhi str. Wilmington |
| ASSOCIATED DISEASE: | Murine typhus (endemic typhus) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RityA.17523.a.B1.GE35399 | Full length( RityA.17523.a ) | 1 | 290 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RityA.17523.a.B1.PS01586 | Full length( RityA.17523.a ) | 1 | 290 |
Structures
4OSE
DEPOSITED: 2/12/2014
DETERMINATION: XRay
CLONE: RityA.17523.a.B1.GE35399
PROTEIN: RityA.17523.a.B1.PS01586
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_152322 |
| PATRIC ID: | fig|257363.4.peg.460 |
| RefSeq: | YP_067390.1 |
| UniProt: | Q68WT4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQINSIKIGP YINLLPLQYI PQHKISYVEF GDPKNKNIIL CAHGLTRNAH DFDKIAKELC KNYRIISINY PGRSDSENLK KPYHYNYTTY IKDTLLFFKR LNIKNPIWLG TSMGGIIGMV LASKYKNIFK ALILNDIGAF IDAAPLIKIG DYAKKTVLLD DLASAKEHLK LIYAQIGIKN EEDWDYLTKY SVISTFGGKY KMNYDPAITK GMQSDNNQED VKLWSVWNKI KCRILVIHGM KSQILTKSTI QKMKKTNTFD LYEIKYAGHA PSLMNDEEIY YIESWLKQKS
NT Sequence
ATGCAAATTA ATTCAATAAA AATTGGGCCC TATATTAATT TATTACCGCT TCAATATATA CCTCAGCATA AAATTTCTTA TGTAGAATTC GGTGATCCTA AAAATAAAAA TATAATATTA TGTGCTCACG GTTTAACTAG AAACGCTCAT GATTTTGATA AAATAGCTAA AGAATTATGC AAAAATTATA GAATAATTTC AATAAATTAT CCTGGTCGCA GTGATAGTGA AAATTTAAAA AAACCTTATC ATTATAATTA TACGACTTAT ATTAAAGATA CGTTACTTTT TTTTAAAAGG CTCAATATCA AAAATCCTAT TTGGCTTGGT ACTTCAATGG GTGGTATTAT AGGTATGGTA CTTGCAAGTA AATATAAAAA TATTTTTAAA GCCTTAATAT TAAATGATAT AGGTGCATTT ATTGATGCTG CACCTTTAAT AAAAATTGGA GATTATGCTA AAAAAACTGT ACTCTTAGAT GATTTAGCTA GTGCAAAAGA ACATTTAAAA CTTATTTATG CTCAAATAGG TATTAAAAAT GAAGAAGATT GGGATTATTT AACAAAATAT AGCGTTATTT CTACATTTGG CGGAAAATAT AAAATGAATT ATGATCCTGC TATAACAAAA GGGATGCAAA GTGATAATAA TCAAGAAGAT GTTAAACTAT GGTCTGTATG GAATAAAATA AAATGTAGGA TATTAGTAAT TCATGGTATG AAATCTCAAA TTTTAACAAA ATCTACCATT CAAAAAATGA AAAAAACAAA CACTTTTGAT CTTTATGAAA TAAAATATGC AGGTCATGCT CCTTCTTTAA TGAATGATGA AGAAATTTAT TATATTGAGT CTTGGTTAAA ACAAAAGTCT
Details for RityA.17523.a.B1.GE35399
| HARVESTED ON: | 5/16/2012 |
| SEQUENCED ON: | 5/15/2012 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMQ INSIKIGPYI NLLPLQYIPQ HKISYVEFGD PKNKNIILCA HGLTRNAHDF DKIAKELCKN YRIISINYPG RSDSENLKKP YHYNYTTYIK DTLLFFKRLN IKNPIWLGTS MGGIIGMVLA SKYKNIFKAL ILNDIGAFID AAPLIKIGDY AKKTVLLDDL ASAKEHLKLI YAQIGIKNEE DWDYLTKYSV ISTFGGKYKM NYDPAITKGM QSDNNQEDVK LWSVWNKIKC RILVIHGMKS QILTKSTIQK MKKTNTFDLY EIKYAGHAPS LMNDEEIYYI ESWLKQKS
Validated NT Sequence
atcttactca cttaagactt ttgttttaac caagactcaa tataataaat ttcttcatca ttcattaaag aaggagcatg acctgcatat tttatttcat aaagatcaaa agtgtttgtt tttttcattt tttgaatggt agattttgtt aaaatttgag atttcatacc atgaattact aatatcctac attttatttt attccataca gaccatagtt taacatcttc ttgattatta tcactttgca tcccttttgt tatagcagga tcataattca ttttatattt tccgccaaat gtagaaataa cgctatattt tgttaaataa tcccaatctt cttcattttt aatacctatt tgagcataaa taagttttaa atgttctttt gcactagcta aatcatctaa gagtacagtt tttttagcat aatctccaat ttttattaaa ggtgcagcat caataaatgc acctatatca tttaatatta aggctttaaa aatattttta tatttacttg caagtaccat acctataata ccacccattg aagtaccaag ccaaatagga tttttgatat tgagcctttt aaaaaaaagt aacgtatctt taatataagt cgtataatta taatgataag gtttttttaa attttcacta tcactgcgac caggataatt tattgaaatt attctataat ttttgcataa ttctttagct attttatcaa aatcatgagc gtttctagtt aaaccgtgag cacataatat tatattttta tttttaggat caccgaattc tacataagaa attttatgct gaggtatata ttgaagcggt aataaattaa tatagggccc aatttttatt gaattaattt gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMQ INSIKIGPYI NLLPLQYIPQ HKISYVEFGD PKNKNIILCA HGLTRNAHDF DKIAKELCKN YRIISINYPG RSDSENLKKP YHYNYTTYIK DTLLFFKRLN IKNPIWLGTS MGGIIGMVLA SKYKNIFKAL ILNDIGAFID AAPLIKIGDY AKKTVLLDDL ASAKEHLKLI YAQIGIKNEE DWDYLTKYSV ISTFGGKYKM NYDPAITKGM QSDNNQEDVK LWSVWNKIKC RILVIHGMKS QILTKSTIQK MKKTNTFDLY EIKYAGHAPS LMNDEEIYYI ESWLKQKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa ttaattcaat aaaaattggg ccctatatta atttattacc gcttcaatat atacctcagc ataaaatttc ttatgtagaa ttcggtgatc ctaaaaataa aaatataata ttatgtgctc acggtttaac tagaaacgct catgattttg ataaaatagc taaagaatta tgcaaaaatt atagaataat ttcaataaat tatcctggtc gcagtgatag tgaaaattta aaaaaacctt atcattataa ttatacgact tatattaaag atacgttact tttttttaaa aggctcaata tcaaaaatcc tatttggctt ggtacttcaa tgggtggtat tataggtatg gtacttgcaa gtaaatataa aaatattttt aaagccttaa tattaaatga tataggtgca tttattgatg ctgcaccttt aataaaaatt ggagattatg ctaaaaaaac tgtactctta gatgatttag ctagtgcaaa agaacattta aaacttattt atgctcaaat aggtattaaa aatgaagaag attgggatta tttaacaaaa tatagcgtta tttctacatt tggcggaaaa tataaaatga attatgatcc tgctataaca aaagggatgc aaagtgataa taatcaagaa gatgttaaac tatggtctgt atggaataaa ataaaatgta ggatattagt aattcatggt atgaaatctc aaattttaac aaaatctacc attcaaaaaa tgaaaaaaac aaacactttt gatctttatg aaataaaata tgcaggtcat gctccttctt taatgaatga tgaagaaatt tattatattg agtcttggtt aaaacaaaag tcttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for RityA.17523.a.B1.PS01586
| PURIFICATION DATe: | 8/7/2012 |
| CONCENTRATION: | 7.07mg/ml |
| OBSERVED MW: | 33kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ INSIKIGPYI NLLPLQYIPQ HKISYVEFGD PKNKNIILCA HGLTRNAHDF DKIAKELCKN YRIISINYPG RSDSENLKKP YHYNYTTYIK DTLLFFKRLN IKNPIWLGTS MGGIIGMVLA SKYKNIFKAL ILNDIGAFID AAPLIKIGDY AKKTVLLDDL ASAKEHLKLI YAQIGIKNEE DWDYLTKYSV ISTFGGKYKM NYDPAITKGM QSDNNQEDVK LWSVWNKIKC RILVIHGMKS QILTKSTIQK MKKTNTFDLY EIKYAGHAPS LMNDEEIYYI ESWLKQKS
Validated NT Sequence
atcttactca cttaagactt ttgttttaac caagactcaa tataataaat ttcttcatca ttcattaaag aaggagcatg acctgcatat tttatttcat aaagatcaaa agtgtttgtt tttttcattt tttgaatggt agattttgtt aaaatttgag atttcatacc atgaattact aatatcctac attttatttt attccataca gaccatagtt taacatcttc ttgattatta tcactttgca tcccttttgt tatagcagga tcataattca ttttatattt tccgccaaat gtagaaataa cgctatattt tgttaaataa tcccaatctt cttcattttt aatacctatt tgagcataaa taagttttaa atgttctttt gcactagcta aatcatctaa gagtacagtt tttttagcat aatctccaat ttttattaaa ggtgcagcat caataaatgc acctatatca tttaatatta aggctttaaa aatattttta tatttacttg caagtaccat acctataata ccacccattg aagtaccaag ccaaatagga tttttgatat tgagcctttt aaaaaaaagt aacgtatctt taatataagt cgtataatta taatgataag gtttttttaa attttcacta tcactgcgac caggataatt tattgaaatt attctataat ttttgcataa ttctttagct attttatcaa aatcatgagc gtttctagtt aaaccgtgag cacataatat tatattttta tttttaggat caccgaattc tacataagaa attttatgct gaggtatata ttgaagcggt aataaattaa tatagggccc aatttttatt gaattaattt gcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMQ INSIKIGPYI NLLPLQYIPQ HKISYVEFGD PKNKNIILCA HGLTRNAHDF DKIAKELCKN YRIISINYPG RSDSENLKKP YHYNYTTYIK DTLLFFKRLN IKNPIWLGTS MGGIIGMVLA SKYKNIFKAL ILNDIGAFID AAPLIKIGDY AKKTVLLDDL ASAKEHLKLI YAQIGIKNEE DWDYLTKYSV ISTFGGKYKM NYDPAITKGM QSDNNQEDVK LWSVWNKIKC RILVIHGMKS QILTKSTIQK MKKTNTFDLY EIKYAGHAPS LMNDEEIYYI ESWLKQKS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA TTAATTCAAT AAAAATTGGG CCCTATATTA ATTTATTACC GCTTCAATAT ATACCTCAGC ATAAAATTTC TTATGTAGAA TTCGGTGATC CTAAAAATAA AAATATAATA TTATGTGCTC ACGGTTTAAC TAGAAACGCT CATGATTTTG ATAAAATAGC TAAAGAATTA TGCAAAAATT ATAGAATAAT TTCAATAAAT TATCCTGGTC GCAGTGATAG TGAAAATTTA AAAAAACCTT ATCATTATAA TTATACGACT TATATTAAAG ATACGTTACT TTTTTTTAAA AGGCTCAATA TCAAAAATCC TATTTGGCTT GGTACTTCAA TGGGTGGTAT TATAGGTATG GTACTTGCAA GTAAATATAA AAATATTTTT AAAGCCTTAA TATTAAATGA TATAGGTGCA TTTATTGATG CTGCACCTTT AATAAAAATT GGAGATTATG CTAAAAAAAC TGTACTCTTA GATGATTTAG CTAGTGCAAA AGAACATTTA AAACTTATTT ATGCTCAAAT AGGTATTAAA AATGAAGAAG ATTGGGATTA TTTAACAAAA TATAGCGTTA TTTCTACATT TGGCGGAAAA TATAAAATGA ATTATGATCC TGCTATAACA AAAGGGATGC AAAGTGATAA TAATCAAGAA GATGTTAAAC TATGGTCTGT ATGGAATAAA ATAAAATGTA GGATATTAGT AATTCATGGT ATGAAATCTC AAATTTTAAC AAAATCTACC ATTCAAAAAA TGAAAAAAAC AAACACTTTT GATCTTTATG AAATAAAATA TGCAGGTCAT GCTCCTTCTT TAATGAATGA TGAAGAAATT TATTATATTG AGTCTTGGTT AAAACAAAAG TCTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT