SSGCID: Target Page

RityA.17430.a

Putative uncharacterized protein

CENTER ID:	RityA.17430.a
ORGANISM:	Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE:	Murine typhus (endemic typhus)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.17430.a.B1.GE35392	Full length( RityA.17430.a )		1	534

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.17430.a.B1.PS01615	Full length( RityA.17430.a )		1	534

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|257363.4.peg.108
RefSeq:	YP_067069.1
UniProt:	Q68XQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIKKANKISY DEVPYPPFTF SYTYPPYLRT IGKLFGLNPP LLETAKVLDI GCGIGVNLLN FAETYPKSQS LGVDLSKTQI ELGKKTISDA KINNVELKAL SILDLDESYG KFDYIVCHGV YSWVSQEVQD KILEVLNKLL NPNGIAFVSY NTLPGWNMQN TIREMMMFHS ESFNTSHDKL QQARLLLKFI NDSLGNSTTP YANFLRDEAK LISTYDDSYV LHEYLGEINT GTYFHQFIEK AQKNHLNYLG DTSIAAMFIG NLPTKAASKL QAINDIVCTE QYMDFITNRK FRSTLLCHQN IPINRKIEFD NLKDFYTTFN IRPISPENKI DLNNEQENIS FYYENLPEPF ISTTSAIMKA ILYVYAENIS NPIRLEQVAK EAFKKLGKYR LQDFLATLEQ HFITLIFQGY LKIFETKPHA IATITEKPKT SQFARYQAKH AHFNNVTNMF SITNRLNDMI GIPIHEKYIL EMLDGTHNID DIKKSIIEKI NSKLLTACDN KGQVVTDPKL LKEFVDYVVA VSLEKFRINY LLVG

NT Sequence

ATGATAAAAA AAGCTAATAA AATATCCTAT GATGAAGTAC CTTATCCTCC ATTTACTTTC AGTTATACCT ATCCACCATA TTTAAGAACT ATCGGTAAGC TATTTGGGCT AAACCCTCCA CTTCTTGAAA CTGCTAAAGT ACTTGATATA GGTTGCGGAA TTGGTGTGAA TCTACTTAAT TTTGCTGAAA CATATCCTAA ATCACAATCT CTTGGCGTTG ATTTATCAAA AACACAAATA GAACTTGGCA AAAAAACTAT CAGTGATGCA AAAATAAACA ATGTCGAACT AAAAGCATTA TCTATATTAG ACCTTGATGA GTCATACGGG AAGTTTGATT ATATAGTTTG CCACGGAGTA TATTCATGGG TATCTCAAGA AGTACAAGAC AAAATACTTG AAGTATTGAA TAAACTGCTA AATCCAAACG GTATAGCTTT TGTAAGTTAT AATACACTCC CAGGCTGGAA TATGCAGAAC ACAATACGTG AAATGATGAT GTTCCATTCA GAATCATTTA ATACTAGCCA TGATAAATTA CAGCAAGCTA GATTACTTTT AAAGTTTATC AATGACTCAT TAGGCAATTC GACAACTCCT TATGCTAATT TCTTAAGAGA TGAAGCAAAA TTAATATCTA CATATGATGA TTCATACGTA TTACATGAAT ATTTAGGCGA AATTAATACA GGTACATATT TCCATCAATT CATAGAGAAA GCTCAAAAAA ACCACCTAAA TTATTTAGGT GATACATCTA TTGCTGCAAT GTTTATCGGT AATTTACCTA CTAAGGCAGC ATCAAAGCTA CAAGCCATCA ATGATATTGT GTGCACTGAA CAATATATGG ATTTTATTAC TAATAGAAAA TTCCGCTCAA CATTGCTCTG TCATCAAAAT ATACCGATAA ACAGAAAAAT TGAATTTGAC AATTTAAAAG ATTTTTACAC TACGTTTAAT ATTAGGCCAA TAAGCCCTGA AAACAAAATT GATTTAAATA ACGAACAGGA AAATATTAGC TTCTATTATG AAAATTTACC TGAGCCTTTT ATTTCAACCA CTTCAGCGAT AATGAAAGCT ATTTTATATG TATATGCTGA AAATATTAGC AATCCTATAA GATTAGAACA AGTAGCAAAA GAAGCCTTTA AAAAGCTTGG TAAATATCGG TTACAAGACT TTTTAGCGAC ATTAGAACAG CATTTTATTA CATTGATTTT TCAAGGTTAT CTTAAAATAT TCGAAACCAA ACCCCATGCT ATCGCTACTA TTACAGAAAA ACCTAAAACA AGTCAATTTG CAAGATACCA AGCAAAACAC GCACATTTTA ATAATGTAAC TAACATGTTT AGTATTACTA ATAGACTTAA TGATATGATA GGTATACCAA TACATGAAAA ATATATTTTA GAAATGTTAG ATGGCACACA TAATATTGAT GATATTAAGA AAAGCATTAT TGAGAAAATT AATTCAAAAC TTTTAACTGC TTGTGACAAT AAAGGACAGG TAGTAACTGA TCCAAAATTA TTAAAAGAAT TTGTTGATTA TGTAGTTGCC GTATCTCTTG AGAAATTTCG TATAAACTAT CTGTTAGTTG GA

Details for RityA.17430.a.B1.GE35392

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	62kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	67

Validated AA Sequence

MQNTIREMMM FHSESFNTSH DKLQQARLLL KFINDSLGNS TTPYANFLRD EAKLISTYDD SYVLHEYLGE INTGTYFHQF IEKAQKNHLN YLGDTSIAAM FIGNLPTKAA SKLQAINDIV CTEQYMDFIT NRKFRSTLLC HQNIPINRKI EFDNLKDFYT TFNIRPISPE NKIDLNNEQE NISFYYENLP EPFISTTSAI MKAILYVYAE NISNPIRLEQ VAKEAFKKLG KYRLQDFLAT LEQHFITLIF QGYLKIFETK PHAIATITEK PKTSQFARYQ AKHAHFNNVT NMFSITNRLN DMIGIPIHEK YILEMLDGTH NIDDIKKSII EKINSKLLTA CDNKGQVVTD PKLLKEFVDY VVAVSLEK

Validated NT Sequence

atttctcaag agatacggca actacataat caacaaattc ttttaataat tttggatcag ttactacctg tcctttattg tcacaagcag ttaaaagttt tgaattaatt ttctcaataa tgcttttctt aatatcatca atattatgtg tgccatctaa catttctaaa atatattttt catgtattgg tatacctatc atatcattaa gtctattagt aatactaaac atgttagtta cattattaaa atgtgcgtgt tttgcttggt atcttgcaaa ttgacttgtt ttaggttttt ctgtaatagt agcgatagca tggggtttgg tttcgaatat tttaagataa ccttgaaaaa tcaatgtaat aaaatgctgt tctaatgtcg ctaaaaagtc ttgtaaccga tatttaccaa gctttttaaa ggcttctttt gctacttgtt ctaatcttat aggattgcta atattttcag catatacata taaaatagct ttcattatcg ctgaagtggt tgaaataaaa ggctcaggta aattttcata atagaagcta atattttcct gttcgttatt taaatcaatt ttgttttcag ggcttattgg cctaatatta aacgtagtgt aaaaatcttt taaattgtca aattcaattt ttctgtttat cggtatattt tgatgacaga gcaatgttga gcggaatttt ctattagtaa taaaatccat atattgttca gtgcacacaa tatcattgat ggcttgtagc tttgatgctg ccttagtagg taaattaccg ataaacattg cagcaataga tgtatcacct aaataattta ggtggttttt ttgagctttc tctatgaatt gatggaaata tgtacctgta ttaatttcgc ctaaatattc atgtaatacg tatgaatcat catatgtaga tattaatttt gcttcatctc ttaagaaatt agcataagga gttgtcgaat tgcctaatga gtcattgata aactttaaaa gtaatctagc ttgctgtaat ttatcatggc tagtattaaa tgattctgaa tggaacatca tcatttcacg tattgtgttc tgcatattcc agcctgggag tgtattataa cttacaaaag ctataccgtt tggatttagc agtttattca atacttcaag tattttgtct tgtacttctt gagataccca tgaatatact ccgtggcaaa ctatataatc aaacttcccg tatgactcat caaggtctaa tatagataat gcttttagtt cgacattgtt tatttttgca tcactgatag tttttttgcc aagttctatt tgtgtttttg ataaatcaac gccaagagat tgtgatttag gatatgtttc agcaaaatta agtagattca caccaattcc gcaacctata tcaagtactt tagcagtttc aagaagtgga gggtttagcc caaatagctt accgatagtt cttaaatatg gtggataggt ataactgaaa gtaaatggag gataaggtac ttcatcatag gatattttat tagctttttt

Expected Protein Sequence

MAHHHHHHMI KKANKISYDE VPYPPFTFSY TYPPYLRTIG KLFGLNPPLL ETAKVLDIGC GIGVNLLNFA ETYPKSQSLG VDLSKTQIEL GKKTISDAKI NNVELKALSI LDLDESYGKF DYIVCHGVYS WVSQEVQDKI LEVLNKLLNP NGIAFVSYNT LPGWNMQNTI REMMMFHSES FNTSHDKLQQ ARLLLKFIND SLGNSTTPYA NFLRDEAKLI STYDDSYVLH EYLGEINTGT YFHQFIEKAQ KNHLNYLGDT SIAAMFIGNL PTKAASKLQA INDIVCTEQY MDFITNRKFR STLLCHQNIP INRKIEFDNL KDFYTTFNIR PISPENKIDL NNEQENISFY YENLPEPFIS TTSAIMKAIL YVYAENISNP IRLEQVAKEA FKKLGKYRLQ DFLATLEQHF ITLIFQGYLK IFETKPHAIA TITEKPKTSQ FARYQAKHAH FNNVTNMFSI TNRLNDMIGI PIHEKYILEM LDGTHNIDDI KKSIIEKINS KLLTACDNKG QVVTDPKLLK EFVDYVVAVS LEKFRINYLL VG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgataa aaaaagctaa taaaatatcc tatgatgaag taccttatcc tccatttact ttcagttata cctatccacc atatttaaga actatcggta agctatttgg gctaaaccct ccacttcttg aaactgctaa agtacttgat ataggttgcg gaattggtgt gaatctactt aattttgctg aaacatatcc taaatcacaa tctcttggcg ttgatttatc aaaaacacaa atagaacttg gcaaaaaaac tatcagtgat gcaaaaataa acaatgtcga actaaaagca ttatctatat tagaccttga tgagtcatac gggaagtttg attatatagt ttgccacgga gtatattcat gggtatctca agaagtacaa gacaaaatac ttgaagtatt gaataaactg ctaaatccaa acggtatagc ttttgtaagt tataatacac tcccaggctg gaatatgcag aacacaatac gtgaaatgat gatgttccat tcagaatcat ttaatactag ccatgataaa ttacagcaag ctagattact tttaaagttt atcaatgact cattaggcaa ttcgacaact ccttatgcta atttcttaag agatgaagca aaattaatat ctacatatga tgattcatac gtattacatg aatatttagg cgaaattaat acaggtacat atttccatca attcatagag aaagctcaaa aaaaccacct aaattattta ggtgatacat ctattgctgc aatgtttatc ggtaatttac ctactaaggc agcatcaaag ctacaagcca tcaatgatat tgtgtgcact gaacaatata tggattttat tactaataga aaattccgct caacattgct ctgtcatcaa aatataccga taaacagaaa aattgaattt gacaatttaa aagattttta cactacgttt aatattaggc caataagccc tgaaaacaaa attgatttaa ataacgaaca ggaaaatatt agcttctatt atgaaaattt acctgagcct tttatttcaa ccacttcagc gataatgaaa gctattttat atgtatatgc tgaaaatatt agcaatccta taagattaga acaagtagca aaagaagcct ttaaaaagct tggtaaatat cggttacaag actttttagc gacattagaa cagcatttta ttacattgat ttttcaaggt tatcttaaaa tattcgaaac caaaccccat gctatcgcta ctattacaga aaaacctaaa acaagtcaat ttgcaagata ccaagcaaaa cacgcacatt ttaataatgt aactaacatg tttagtatta ctaatagact taatgatatg ataggtatac caatacatga aaaatatatt ttagaaatgt tagatggcac acataatatt gatgatatta agaaaagcat tattgagaaa attaattcaa aacttttaac tgcttgtgac aataaaggac aggtagtaac tgatccaaaa ttattaaaag aatttgttga ttatgtagtt gccgtatctc ttgagaaatt tcgtataaac tatctgttag ttggatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for RityA.17430.a.B1.PS01615

PURIFICATION DATe:	9/5/2012
CONCENTRATION:	45.95mg/ml
OBSERVED MW:	64kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	67

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATAA AAAAAGCTAA TAAAATATCC TATGATGAAG TACCTTATCC TCCATTTACT TTCAGTTATA CCTATCCACC ATATTTAAGA ACTATCGGTA AGCTATTTGG GCTAAACCCT CCACTTCTTG AAACTGCTAA AGTACTTGAT ATAGGTTGCG GAATTGGTGT GAATCTACTT AATTTTGCTG AAACATATCC TAAATCACAA TCTCTTGGCG TTGATTTATC AAAAACACAA ATAGAACTTG GCAAAAAAAC TATCAGTGAT GCAAAAATAA ACAATGTCGA ACTAAAAGCA TTATCTATAT TAGACCTTGA TGAGTCATAC GGGAAGTTTG ATTATATAGT TTGCCACGGA GTATATTCAT GGGTATCTCA AGAAGTACAA GACAAAATAC TTGAAGTATT GAATAAACTG CTAAATCCAA ACGGTATAGC TTTTGTAAGT TATAATACAC TCCCAGGCTG GAATATGCAG AACACAATAC GTGAAATGAT GATGTTCCAT TCAGAATCAT TTAATACTAG CCATGATAAA TTACAGCAAG CTAGATTACT TTTAAAGTTT ATCAATGACT CATTAGGCAA TTCGACAACT CCTTATGCTA ATTTCTTAAG AGATGAAGCA AAATTAATAT CTACATATGA TGATTCATAC GTATTACATG AATATTTAGG CGAAATTAAT ACAGGTACAT ATTTCCATCA ATTCATAGAG AAAGCTCAAA AAAACCACCT AAATTATTTA GGTGATACAT CTATTGCTGC AATGTTTATC GGTAATTTAC CTACTAAGGC AGCATCAAAG CTACAAGCCA TCAATGATAT TGTGTGCACT GAACAATATA TGGATTTTAT TACTAATAGA AAATTCCGCT CAACATTGCT CTGTCATCAA AATATACCGA TAAACAGAAA AATTGAATTT GACAATTTAA AAGATTTTTA CACTACGTTT AATATTAGGC CAATAAGCCC TGAAAACAAA ATTGATTTAA ATAACGAACA GGAAAATATT AGCTTCTATT ATGAAAATTT ACCTGAGCCT TTTATTTCAA CCACTTCAGC GATAATGAAA GCTATTTTAT ATGTATATGC TGAAAATATT AGCAATCCTA TAAGATTAGA ACAAGTAGCA AAAGAAGCCT TTAAAAAGCT TGGTAAATAT CGGTTACAAG ACTTTTTAGC GACATTAGAA CAGCATTTTA TTACATTGAT TTTTCAAGGT TATCTTAAAA TATTCGAAAC CAAACCCCAT GCTATCGCTA CTATTACAGA AAAACCTAAA ACAAGTCAAT TTGCAAGATA CCAAGCAAAA CACGCACATT TTAATAATGT AACTAACATG TTTAGTATTA CTAATAGACT TAATGATATG ATAGGTATAC CAATACATGA AAAATATATT TTAGAAATGT TAGATGGCAC ACATAATATT GATGATATTA AGAAAAGCAT TATTGAGAAA ATTAATTCAA AACTTTTAAC TGCTTGTGAC AATAAAGGAC AGGTAGTAAC TGATCCAAAA TTATTAAAAG AATTTGTTGA TTATGTAGTT GCCGTATCTC TTGAGAAATT TCGTATAAAC TATCTGTTAG TTGGATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT