RityA.10201.a

Methionyl-tRNA synthetase

CENTER ID: RityA.10201.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.10201.a.B1.GE35387 Full length( RityA.10201.a ) 1 507
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.10201.a.B1.PW36011 Full length( RityA.10201.a ) 1 507

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127468
PATRIC ID: fig|257363.4.peg.697
RefSeq: YP_067620.1
UniProt: Q68W54

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNTYYITTP IYYVNDVSHI GHAYTSVASD VIARFMRCCG KDVMFLTGTD EHGQKVEKAA INKNIDPQSF TDKTSQNFRD LMVAMNISND DFIRTTENRH KKAVAVFWQK LLDNGAIYEG FYEGWYSVRD EAFYDESEIT EDKLAPTGAP VEWVKEPSYF FNLAKWQDKL LEFYELNPDF VRPISRRNEV ISFVKSGLKD LSISRTTFNW GIKVPNNEKH VIYVWLDALV NYISALGYPD QQSNYSKFWP ANLQVVGKDI LRFHAVYWPA FLMAAEISPP KSIMVHGWWT NAGQKISKSL GNTIDPITLI DEFGVDQVRY FLMREVIFGA DANFTRNNLI TRINSELSNK IGNLLHRIVS FVYNNNDAKV PLIKSGVIDK IYELPILKTA MKFAQENILL MDKIEINKIL ENIINLAEDA NIYIANEAPW NLKKTDYDKM LEVLYTLLEV LRYVAIMLQP FVPSSANKML DQLGVAKEER LFKHLVRDHA LKAGSNILEP SIIFPKI
NT Sequence
ATGAAAAATA CTTACTATAT TACTACCCCT ATATATTATG TTAATGATGT TTCCCATATT GGTCATGCTT ATACTAGTGT TGCAAGTGAT GTGATTGCCC GTTTTATGCG TTGTTGCGGT AAGGATGTAA TGTTTTTAAC AGGTACTGAT GAACATGGAC AGAAAGTAGA AAAAGCAGCT ATTAATAAAA ATATTGATCC TCAATCATTT ACTGATAAGA CATCACAAAA TTTTCGTGAT CTTATGGTTG CAATGAATAT TTCTAATGAT GATTTTATTA GGACAACTGA AAATAGACAT AAAAAAGCTG TAGCAGTTTT TTGGCAGAAA TTACTAGATA ATGGTGCAAT CTATGAAGGA TTTTATGAGG GTTGGTATTC AGTGCGTGAT GAGGCTTTTT ATGATGAATC AGAGATCACT GAAGATAAAT TAGCACCGAC TGGAGCACCT GTTGAATGGG TTAAAGAGCC AAGCTATTTC TTTAATCTTG CAAAATGGCA AGATAAATTA CTTGAGTTTT ATGAGCTAAA CCCTGATTTT GTCAGACCAA TATCAAGGCG TAATGAAGTG ATTAGCTTTG TTAAATCAGG TCTAAAGGAT TTATCCATAT CACGTACTAC TTTTAATTGG GGGATCAAAG TTCCAAATAA TGAAAAACAT GTGATTTATG TTTGGCTTGA TGCGCTTGTT AATTATATTT CAGCACTTGG TTATCCTGAT CAACAAAGTA ATTATAGTAA ATTTTGGCCT GCTAATTTAC AAGTAGTAGG TAAGGATATC TTGCGTTTTC ACGCTGTATA TTGGCCTGCT TTCTTAATGG CTGCAGAAAT TTCACCGCCG AAGTCAATTA TGGTTCATGG TTGGTGGACT AATGCGGGAC AGAAGATTTC TAAATCTCTT GGTAATACCA TTGATCCGAT TACATTAATC GATGAATTCG GTGTTGATCA GGTGAGGTAT TTTCTTATGC GTGAAGTAAT TTTTGGTGCA GATGCTAATT TTACTCGTAA TAATTTAATT ACACGTATTA ATAGCGAACT TTCAAATAAA ATAGGTAATT TATTACATCG TATTGTATCT TTTGTGTATA ACAATAATGA TGCTAAAGTG CCTTTGATTA AGTCAGGTGT TATAGATAAA ATATATGAGC TCCCGATTTT AAAAACCGCA ATGAAATTTG CTCAAGAGAA TATTTTATTG ATGGATAAAA TAGAGATTAA TAAAATTCTT GAAAATATTA TTAATTTGGC TGAAGATGCA AATATCTATA TTGCTAATGA AGCGCCTTGG AATTTAAAAA AAACTGATTA TGATAAGATG TTAGAGGTGT TATATACTCT GCTAGAAGTA CTACGTTATG TTGCAATAAT GCTTCAGCCT TTTGTACCAA GTTCCGCAAA TAAAATGCTA GATCAGTTAG GGGTTGCTAA AGAAGAGAGA TTATTTAAGC ATCTAGTACG TGATCATGCT TTAAAAGCAG GTAGTAATAT TTTAGAGCCT AGTATAATTT TTCCAAAAAT T
Details for RityA.10201.a.B1.GE35387
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 59kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Validated AA Sequence
MRCCGKDVMF LTGTDEHGQK VEKAAINKNI DPQSFTDKTS QNFRDLMVAM NISNDDFIRT TENRHKKAVA VFWQKLLDNG AIYEGFYEGW YSVRDEAFYD ESEITEDKLA PTGAPVEWVK EPSYFFNLAK WQDKLLEFYE LNPDFVRPIS RRNEVISFVK SGLKDLSISR TTFNWGIKVP NNEKHVIYVW LDALVNYISA LGYPDQQSNY SKFWPANLQV VGKDILRFHA VYWPAFLMAA EISPPKSIMV HGWWTNAGQK ISKSLGNTID PITLIDEFGV DQVRYFLMRE VIFGADANFT RNNLITRINS ELSNKIGNLL HRIVSFVYNN NDAKVPLIKS GVIDKIYELP ILKTAMKFAQ ENILLMDKIE INKILENIIN LAEDANIYIA NEAPWNLKKT DYDKMLEVLY TLLEVLRYVA IMLQPFVPSS ANKMLDQLGV AKEERLFKHL VRDHALKAGS NIL
Validated NT Sequence
ctaaaatatt actacctgct tttaaagcat gatcacgtac tagatgctta aataatctct cttctttagc aacccctaac tgatctagca ttttatttgc ggaacttggt acaaaaggct gaagcattat tgcaacataa cgtagtactt ctagcagagt atataacacc tctaacatct tatcataatc agtttttttt aaattccaag gcgcttcatt agcaatatag atatttgcat cttcagccaa attaataata ttttcaagaa ttttattaat ctctatttta tccatcaata aaatattctc ttgagcaaat ttcattgcgg tttttaaaat cgggagctca tatattttat ctataacacc tgacttaatc aaaggcactt tagcatcatt attgttatac acaaaagata caatacgatg taataaatta cctattttat ttgaaagttc gctattaata cgtgtaatta aattattacg agtaaaatta gcatctgcac caaaaattac ttcacgcata agaaaatacc tcacctgatc aacaccgaat tcatcgatta atgtaatcgg atcaatggta ttaccaagag atttagaaat cttctgtccc gcattagtcc accaaccatg aaccataatt gacttcggcg gtgaaatttc tgcagccatt aagaaagcag gccaatatac agcgtgaaaa cgcaagatat ccttacctac tacttgtaaa ttagcaggcc aaaatttact ataattactt tgttgatcag gataaccaag tgctgaaata taattaacaa gcgcatcaag ccaaacataa atcacatgtt tttcattatt tggaactttg atcccccaat taaaagtagt acgtgatatg gataaatcct ttagacctga tttaacaaag ctaatcactt cattacgcct tgatattggt ctgacaaaat cagggtttag ctcataaaac tcaagtaatt tatcttgcca ttttgcaaga ttaaagaaat agcttggctc tttaacccat tcaacaggtg ctccagtcgg tgctaattta tcttcagtga tctctgattc atcataaaaa gcctcatcac gcactgaata ccaaccctca taaaatcctt catagattgc accattatct agtaatttct gccaaaaaac tgctacagct tttttatgtc tattttcagt tgtcctaata aaatcatcat tagaaatatt cattgcaacc ataagatcac gaaaattttg tgatgtctta tcagtaaatg attgaggatc aatattttta ttaatagctg ctttttctac tttctgtcca tgttcatcag tacctgttaa aaacattaca tccttaccgc aacaacgcat aaaacgggca atcacatcac ttgcaacact agtataagca tgaccaatat gggaaacatc attaacataa tatatagggg tagtaatata gtaagtat
Expected Protein Sequence
MAHHHHHHMK NTYYITTPIY YVNDVSHIGH AYTSVASDVI ARFMRCCGKD VMFLTGTDEH GQKVEKAAIN KNIDPQSFTD KTSQNFRDLM VAMNISNDDF IRTTENRHKK AVAVFWQKLL DNGAIYEGFY EGWYSVRDEA FYDESEITED KLAPTGAPVE WVKEPSYFFN LAKWQDKLLE FYELNPDFVR PISRRNEVIS FVKSGLKDLS ISRTTFNWGI KVPNNEKHVI YVWLDALVNY ISALGYPDQQ SNYSKFWPAN LQVVGKDILR FHAVYWPAFL MAAEISPPKS IMVHGWWTNA GQKISKSLGN TIDPITLIDE FGVDQVRYFL MREVIFGADA NFTRNNLITR INSELSNKIG NLLHRIVSFV YNNNDAKVPL IKSGVIDKIY ELPILKTAMK FAQENILLMD KIEINKILEN IINLAEDANI YIANEAPWNL KKTDYDKMLE VLYTLLEVLR YVAIMLQPFV PSSANKMLDQ LGVAKEERLF KHLVRDHALK AGSNILEPSI IFPKI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa atacttacta tattactacc cctatatatt atgttaatga tgtttcccat attggtcatg cttatactag tgttgcaagt gatgtgattg cccgttttat gcgttgttgc ggtaaggatg taatgttttt aacaggtact gatgaacatg gacagaaagt agaaaaagca gctattaata aaaatattga tcctcaatca tttactgata agacatcaca aaattttcgt gatcttatgg ttgcaatgaa tatttctaat gatgatttta ttaggacaac tgaaaataga cataaaaaag ctgtagcagt tttttggcag aaattactag ataatggtgc aatctatgaa ggattttatg agggttggta ttcagtgcgt gatgaggctt tttatgatga atcagagatc actgaagata aattagcacc gactggagca cctgttgaat gggttaaaga gccaagctat ttctttaatc ttgcaaaatg gcaagataaa ttacttgagt tttatgagct aaaccctgat tttgtcagac caatatcaag gcgtaatgaa gtgattagct ttgttaaatc aggtctaaag gatttatcca tatcacgtac tacttttaat tgggggatca aagttccaaa taatgaaaaa catgtgattt atgtttggct tgatgcgctt gttaattata tttcagcact tggttatcct gatcaacaaa gtaattatag taaattttgg cctgctaatt tacaagtagt aggtaaggat atcttgcgtt ttcacgctgt atattggcct gctttcttaa tggctgcaga aatttcaccg ccgaagtcaa ttatggttca tggttggtgg actaatgcgg gacagaagat ttctaaatct cttggtaata ccattgatcc gattacatta atcgatgaat tcggtgttga tcaggtgagg tattttctta tgcgtgaagt aatttttggt gcagatgcta attttactcg taataattta attacacgta ttaatagcga actttcaaat aaaataggta atttattaca tcgtattgta tcttttgtgt ataacaataa tgatgctaaa gtgcctttga ttaagtcagg tgttatagat aaaatatatg agctcccgat tttaaaaacc gcaatgaaat ttgctcaaga gaatatttta ttgatggata aaatagagat taataaaatt cttgaaaata ttattaattt ggctgaagat gcaaatatct atattgctaa tgaagcgcct tggaatttaa aaaaaactga ttatgataag atgttagagg tgttatatac tctgctagaa gtactacgtt atgttgcaat aatgcttcag ccttttgtac caagttccgc aaataaaatg ctagatcagt taggggttgc taaagaagag agattattta agcatctagt acgtgatcat gctttaaaag caggtagtaa tattttagag cctagtataa tttttccaaa aatttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for RityA.10201.a.B1.PW36011
PURIFICATION DATe: 7/30/2012
CONCENTRATION: 29.22mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MRCCGKDVMF LTGTDEHGQK VEKAAINKNI DPQSFTDKTS QNFRDLMVAM NISNDDFIRT TENRHKKAVA VFWQKLLDNG AIYEGFYEGW YSVRDEAFYD ESEITEDKLA PTGAPVEWVK EPSYFFNLAK WQDKLLEFYE LNPDFVRPIS RRNEVISFVK SGLKDLSISR TTFNWGIKVP NNEKHVIYVW LDALVNYISA LGYPDQQSNY SKFWPANLQV VGKDILRFHA VYWPAFLMAA EISPPKSIMV HGWWTNAGQK ISKSLGNTID PITLIDEFGV DQVRYFLMRE VIFGADANFT RNNLITRINS ELSNKIGNLL HRIVSFVYNN NDAKVPLIKS GVIDKIYELP ILKTAMKFAQ ENILLMDKIE INKILENIIN LAEDANIYIA NEAPWNLKKT DYDKMLEVLY TLLEVLRYVA IMLQPFVPSS ANKMLDQLGV AKEERLFKHL VRDHALKAGS NIL
Validated NT Sequence
ctaaaatatt actacctgct tttaaagcat gatcacgtac tagatgctta aataatctct cttctttagc aacccctaac tgatctagca ttttatttgc ggaacttggt acaaaaggct gaagcattat tgcaacataa cgtagtactt ctagcagagt atataacacc tctaacatct tatcataatc agtttttttt aaattccaag gcgcttcatt agcaatatag atatttgcat cttcagccaa attaataata ttttcaagaa ttttattaat ctctatttta tccatcaata aaatattctc ttgagcaaat ttcattgcgg tttttaaaat cgggagctca tatattttat ctataacacc tgacttaatc aaaggcactt tagcatcatt attgttatac acaaaagata caatacgatg taataaatta cctattttat ttgaaagttc gctattaata cgtgtaatta aattattacg agtaaaatta gcatctgcac caaaaattac ttcacgcata agaaaatacc tcacctgatc aacaccgaat tcatcgatta atgtaatcgg atcaatggta ttaccaagag atttagaaat cttctgtccc gcattagtcc accaaccatg aaccataatt gacttcggcg gtgaaatttc tgcagccatt aagaaagcag gccaatatac agcgtgaaaa cgcaagatat ccttacctac tacttgtaaa ttagcaggcc aaaatttact ataattactt tgttgatcag gataaccaag tgctgaaata taattaacaa gcgcatcaag ccaaacataa atcacatgtt tttcattatt tggaactttg atcccccaat taaaagtagt acgtgatatg gataaatcct ttagacctga tttaacaaag ctaatcactt cattacgcct tgatattggt ctgacaaaat cagggtttag ctcataaaac tcaagtaatt tatcttgcca ttttgcaaga ttaaagaaat agcttggctc tttaacccat tcaacaggtg ctccagtcgg tgctaattta tcttcagtga tctctgattc atcataaaaa gcctcatcac gcactgaata ccaaccctca taaaatcctt catagattgc accattatct agtaatttct gccaaaaaac tgctacagct tttttatgtc tattttcagt tgtcctaata aaatcatcat tagaaatatt cattgcaacc ataagatcac gaaaattttg tgatgtctta tcagtaaatg attgaggatc aatattttta ttaatagctg ctttttctac tttctgtcca tgttcatcag tacctgttaa aaacattaca tccttaccgc aacaacgcat aaaacgggca atcacatcac ttgcaacact agtataagca tgaccaatat gggaaacatc attaacataa tatatagggg tagtaatata gtaagtat
Expressed Protein Sequence
MAHHHHHHMK NTYYITTPIY YVNDVSHIGH AYTSVASDVI ARFMRCCGKD VMFLTGTDEH GQKVEKAAIN KNIDPQSFTD KTSQNFRDLM VAMNISNDDF IRTTENRHKK AVAVFWQKLL DNGAIYEGFY EGWYSVRDEA FYDESEITED KLAPTGAPVE WVKEPSYFFN LAKWQDKLLE FYELNPDFVR PISRRNEVIS FVKSGLKDLS ISRTTFNWGI KVPNNEKHVI YVWLDALVNY ISALGYPDQQ SNYSKFWPAN LQVVGKDILR FHAVYWPAFL MAAEISPPKS IMVHGWWTNA GQKISKSLGN TIDPITLIDE FGVDQVRYFL MREVIFGADA NFTRNNLITR INSELSNKIG NLLHRIVSFV YNNNDAKVPL IKSGVIDKIY ELPILKTAMK FAQENILLMD KIEINKILEN IINLAEDANI YIANEAPWNL KKTDYDKMLE VLYTLLEVLR YVAIMLQPFV PSSANKMLDQ LGVAKEERLF KHLVRDHALK AGSNILEPSI IFPKI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA ATACTTACTA TATTACTACC CCTATATATT ATGTTAATGA TGTTTCCCAT ATTGGTCATG CTTATACTAG TGTTGCAAGT GATGTGATTG CCCGTTTTAT GCGTTGTTGC GGTAAGGATG TAATGTTTTT AACAGGTACT GATGAACATG GACAGAAAGT AGAAAAAGCA GCTATTAATA AAAATATTGA TCCTCAATCA TTTACTGATA AGACATCACA AAATTTTCGT GATCTTATGG TTGCAATGAA TATTTCTAAT GATGATTTTA TTAGGACAAC TGAAAATAGA CATAAAAAAG CTGTAGCAGT TTTTTGGCAG AAATTACTAG ATAATGGTGC AATCTATGAA GGATTTTATG AGGGTTGGTA TTCAGTGCGT GATGAGGCTT TTTATGATGA ATCAGAGATC ACTGAAGATA AATTAGCACC GACTGGAGCA CCTGTTGAAT GGGTTAAAGA GCCAAGCTAT TTCTTTAATC TTGCAAAATG GCAAGATAAA TTACTTGAGT TTTATGAGCT AAACCCTGAT TTTGTCAGAC CAATATCAAG GCGTAATGAA GTGATTAGCT TTGTTAAATC AGGTCTAAAG GATTTATCCA TATCACGTAC TACTTTTAAT TGGGGGATCA AAGTTCCAAA TAATGAAAAA CATGTGATTT ATGTTTGGCT TGATGCGCTT GTTAATTATA TTTCAGCACT TGGTTATCCT GATCAACAAA GTAATTATAG TAAATTTTGG CCTGCTAATT TACAAGTAGT AGGTAAGGAT ATCTTGCGTT TTCACGCTGT ATATTGGCCT GCTTTCTTAA TGGCTGCAGA AATTTCACCG CCGAAGTCAA TTATGGTTCA TGGTTGGTGG ACTAATGCGG GACAGAAGAT TTCTAAATCT CTTGGTAATA CCATTGATCC GATTACATTA ATCGATGAAT TCGGTGTTGA TCAGGTGAGG TATTTTCTTA TGCGTGAAGT AATTTTTGGT GCAGATGCTA ATTTTACTCG TAATAATTTA ATTACACGTA TTAATAGCGA ACTTTCAAAT AAAATAGGTA ATTTATTACA TCGTATTGTA TCTTTTGTGT ATAACAATAA TGATGCTAAA GTGCCTTTGA TTAAGTCAGG TGTTATAGAT AAAATATATG AGCTCCCGAT TTTAAAAACC GCAATGAAAT TTGCTCAAGA GAATATTTTA TTGATGGATA AAATAGAGAT TAATAAAATT CTTGAAAATA TTATTAATTT GGCTGAAGAT GCAAATATCT ATATTGCTAA TGAAGCGCCT TGGAATTTAA AAAAAACTGA TTATGATAAG ATGTTAGAGG TGTTATATAC TCTGCTAGAA GTACTACGTT ATGTTGCAAT AATGCTTCAG CCTTTTGTAC CAAGTTCCGC AAATAAAATG CTAGATCAGT TAGGGGTTGC TAAAGAAGAG AGATTATTTA AGCATCTAGT ACGTGATCAT GCTTTAAAAG CAGGTAGTAA TATTTTAGAG CCTAGTATAA TTTTTCCAAA AATTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT