SSGCID: Target Page

RityA.10014.d

ABC transporter ATP binding protein

CENTER ID:	RityA.10014.d
ORGANISM:	Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE:	Murine typhus (endemic typhus)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.10014.d.B1.GE35376	Full length( RityA.10014.d )		1	246

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|257363.4.peg.373
RefSeq:	YP_067318.1
UniProt:	Q68X06

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPYLYAEKI QFKVSERSDP IIFETTLNIA QEEFVVILGH NGSGKSTLAK ILAGYLTPTS GQVFLDQVKV DKISQKHKAL MLVTITQKLE DRLFTELTLE ENIMLWESRY HSNERLTSSD VLELTGSPKR FLPLLSQQLG KFSGGEKQVI LLALSIAHPP KILFLDEHTA NLDPKASLEV IKKTAEIIEH HKITAVMITH NLEAAAHYGK RLIVLDSGKV VLDYLKPQNF SLRELRAILS SKIAGW

NT Sequence

ATGAAACCTT ATTTATATGC AGAAAAAATA CAGTTCAAAG TTAGTGAAAG AAGTGATCCT ATAATTTTCG AGACTACTTT AAATATTGCA CAAGAAGAGT TTGTGGTAAT ATTAGGTCAT AACGGTAGTG GTAAATCTAC TTTAGCTAAA ATACTTGCCG GTTATTTAAC ACCGACAAGC GGACAAGTAT TCTTAGATCA AGTTAAAGTT GATAAAATAT CTCAAAAACA CAAAGCACTT ATGCTTGTTA CAATAACACA AAAGCTTGAA GATAGGCTAT TTACTGAGTT AACTCTTGAG GAAAATATTA TGCTTTGGGA AAGTAGATAC CATAGTAATG AACGGTTAAC AAGTAGTGAT GTATTAGAGC TTACAGGTTC GCCTAAACGC TTTTTACCTT TACTTTCGCA GCAACTAGGT AAATTCTCAG GTGGAGAAAA GCAGGTTATA TTGCTTGCAC TTAGTATAGC TCATCCACCT AAAATATTAT TTCTAGATGA GCATACGGCT AATTTAGATC CGAAAGCTTC TTTGGAAGTG ATAAAAAAAA CAGCAGAGAT TATAGAGCAT CATAAGATAA CTGCCGTTAT GATTACGCAT AATTTGGAAG CGGCAGCACA TTATGGCAAA AGGCTTATAG TACTTGATAG TGGTAAGGTA GTGCTAGACT ACCTAAAACC ACAAAATTTT TCTTTAAGAG AACTTAGAGC TATATTATCA TCTAAGATCG CAGGGTGG

Details for RityA.10014.d.B1.GE35376

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK PYLYAEKIQF KVSERSDPII FETTLNIAQE EFVVILGHNG SGKSTLAKIL AGYLTPTSGQ VFLDQVKVDK ISQKHKALML VTITQKLEDR LFTELTLEEN IMLWESRYHS NERLTSSDVL ELTGSPKRFL PLLSQQLGKF SGGEKQVILL ALSIAHPPKI LFLDEHTANL DPKASLEVIK KTAEIIEHHK ITAVMITHNL EAAAHYGKRL IVLDSGKVVL DYLKPQNFSL RELRAILSSK IAGW

Validated NT Sequence

atcttactca cttaccaccc tgcgatctta gatgataata tagctctaag ttctcttaaa gaaaaatttt gtggttttag gtagtctagc actaccttac cactatcaag tactataagc cttttgccat aatgtgctgc cgcttccaaa ttatgcgtaa tcataacggc agttatctta tgatgctcta taatctctgc tgtttttttt atcacttcca aagaagcttt cggatctaaa ttagccgtat gctcatctag aaataatatt ttaggtggat gagctatact aagtgcaagc aatataacct gcttttctcc acctgagaat ttacctagtt gctgcgaaag taaaggtaaa aagcgtttag gcgaacctgt aagctctaat acatcactac ttgttaaccg ttcattacta tggtatctac tttcccaaag cataatattt tcctcaagag ttaactcagt aaatagccta tcttcaagct tttgtgttat tgtaacaagc ataagtgctt tgtgtttttg agatatttta tcaactttaa cttgatctaa gaatacttgt ccgcttgtcg gtgttaaata accggcaagt attttagcta aagtagattt accactaccg ttatgaccta atattaccac aaactcttct tgtgcaatat ttaaagtagt ctcgaaaatt ataggatcac ttctttcact aactttgaac tgtatttttt ctgcatataa ataaggtttc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac cttatttata tgcagaaaaa atacagttca aagttagtga aagaagtgat cctataattt tcgagactac tttaaatatt gcacaagaag agtttgtggt aatattaggt cataacggta gtggtaaatc tactttagct aaaatacttg ccggttattt aacaccgaca agcggacaag tattcttaga tcaagttaaa gttgataaaa tatctcaaaa acacaaagca cttatgcttg ttacaataac acaaaagctt gaagataggc tatttactga gttaactctt gaggaaaata ttatgctttg ggaaagtaga taccatagta atgaacggtt aacaagtagt gatgtattag agcttacagg ttcgcctaaa cgctttttac ctttactttc gcagcaacta ggtaaattct caggtggaga aaagcaggtt atattgcttg cacttagtat agctcatcca cctaaaatat tatttctaga tgagcatacg gctaatttag atccgaaagc ttctttggaa gtgataaaaa aaacagcaga gattatagag catcataaga taactgccgt tatgattacg cataatttgg aagcggcagc acattatggc aaaaggctta tagtacttga tagtggtaag gtagtgctag actacctaaa accacaaaat ttttctttaa gagaacttag agctatatta tcatctaaga tcgcagggtg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat