SSGCID: Target Page

RityA.10014.c

Lipoprotein-releasing system ATP-binding protein LolD

CENTER ID:	RityA.10014.c
ORGANISM:	Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE:	Murine typhus (endemic typhus)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.10014.c.B1.GE35377	Full length( RityA.10014.c )		1	221

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|257363.4.peg.713
RefSeq:	YP_067636.1
UniProt:	Q68W38

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNTVLILKK ISKHYKQGNT IVKVLDDLNL TANEGELIAI IGSSGSGKST LLHIAGLLDK PTNGQVIIPN SKYKKYHLIR LYYLGFIYQQ HHLLKDFTAL ENVIIPRLIR GLDQKEAIRD ATKILDDLGL EKKLYNMPGE LSGGEQQRVA IARSLINKPR IILADEPTGN LDPKSTNEVF NLFLKVAQKQ NTTVIMVTHN HELAHKMDKL YNLKNGLLNI A

NT Sequence

ATGAATAATA CAGTCCTCAT TTTAAAAAAA ATCTCAAAAC ATTATAAACA AGGCAACACT ATTGTTAAAG TACTAGATGA TCTTAATCTA ACTGCTAATG AAGGTGAATT AATCGCTATC ATCGGTTCTT CAGGTAGTGG CAAATCAACT TTACTACATA TTGCAGGCTT ACTTGATAAG CCTACAAACG GTCAAGTCAT AATACCAAAT AGCAAGTATA AAAAATACCA TCTAATTCGT CTTTATTACT TAGGTTTTAT CTATCAACAA CATCATTTAC TTAAAGATTT TACTGCTCTT GAAAATGTTA TCATACCAAG ATTGATTAGA GGTCTTGATC AAAAAGAAGC AATAAGAGAT GCAACAAAGA TATTAGATGA TTTAGGACTT GAGAAAAAAC TCTATAATAT GCCTGGAGAA TTATCGGGAG GAGAGCAACA AAGGGTCGCT ATAGCTCGCA GCTTAATTAA TAAGCCGAGA ATTATTTTAG CTGATGAGCC AACAGGTAAT TTAGATCCAA AAAGCACAAA TGAAGTATTT AATTTATTTT TAAAAGTTGC ACAAAAACAA AATACTACAG TTATTATGGT AACACATAAC CATGAGTTAG CACATAAAAT GGATAAATTA TATAATCTAA AGAATGGATT ATTAAATATA GCT

Details for RityA.10014.c.B1.GE35377

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN NTVLILKKIS KHYKQGNTIV KVLDDLNLTA NEGELIAIIG SSGSGKSTLL HIAGLLDKPT NGQVIIPNSK YKKYHLIRLY YLGFIYQQHH LLKDFTALEN VIIPRLIRGL DQKEAIRDAT KILDDLGLEK KLYNMPGELS GGEQQRVAIA RSLINKPRII LADEPTGNLD PKSTNEVFNL FLKVAQKQNT TVIMVTHNHE LAHKMDKLYN LKNGLLNIA

Validated NT Sequence

tttgtnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgaataa tacagtcctc attttaaaaa aaatctcaaa acattataaa caaggcaaca ctattgttaa agtactagat gatcttaatc taactgctaa tgaaggtgaa ttaatcgcta tcatcggttc ttcaggtagt ggcaaatcaa ctttactaca tattgcaggc ttacttgata agcctacaaa cggtcaagtc ataataccaa atagcaagta taaaaaatac catctaattc gtctttatta cttaggtttt atctatcaac aacatcattt acttaaagat tttactgctc ttgaaaatgt tatcatacca agattgatta gaggtcttga tcaaaaagaa gcaataagag atgcaacaaa gatattagat gatttaggac ttgagaaaaa actctataat atgcctggag aattatcggg aggagagcaa caaagggtcg ctatagctcg cagcttaatt aataagccga gaattatttt agctgatgag ccaacaggta atttagatcc aaaaagcaca aatgaagtat ttaatttatt tttaaaagtt gcacaaaaac aaaatactac agttattatg gtaacacata accatgagtt agcacataaa atggataaat tatataatct aaagaatgga ttattaaata tagcttaagt gagtaagata ggatccggct gctaacaann cccgaaag

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata atacagtcct cattttaaaa aaaatctcaa aacattataa acaaggcaac actattgtta aagtactaga tgatcttaat ctaactgcta atgaaggtga attaatcgct atcatcggtt cttcaggtag tggcaaatca actttactac atattgcagg cttacttgat aagcctacaa acggtcaagt cataatacca aatagcaagt ataaaaaata ccatctaatt cgtctttatt acttaggttt tatctatcaa caacatcatt tacttaaaga ttttactgct cttgaaaatg ttatcatacc aagattgatt agaggtcttg atcaaaaaga agcaataaga gatgcaacaa agatattaga tgatttagga cttgagaaaa aactctataa tatgcctgga gaattatcgg gaggagagca acaaagggtc gctatagctc gcagcttaat taataagccg agaattattt tagctgatga gccaacaggt aatttagatc caaaaagcac aaatgaagta tttaatttat ttttaaaagt tgcacaaaaa caaaatacta cagttattat ggtaacacat aaccatgagt tagcacataa aatggataaa ttatataatc taaagaatgg attattaaat atagcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t