SSGCID: Target Page

RityA.10014.b

Cytochrome c biogenesis ATP-binding export protein CcmA

CENTER ID:	RityA.10014.b
ORGANISM:	Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE:	Murine typhus (endemic typhus)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.10014.b.B1.GE35378	Full length( RityA.10014.b )		1	197

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|257363.4.peg.800
RefSeq:	YP_067719.1
UniProt:	Q68VV5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSMLSLHQLQ LNIEKRNLFD LCITFLPSAI TYIKGANGSG KSSLLRMIAG IMQPSSGNIY YKNSHINNCQ KPYCTYIGHN LGIKLEMTVF ENLKFWSEIY NSTETVQAAI HYFKLDYLLD KKCYNLSSGI QKVVAIARLI VCKSDLWLLD EVETNLSKEN RDLLNNLIIM KANSGGIILL SSHQESVIKS AQILQLK

NT Sequence

ATGAGTATGT TATCACTTCA TCAATTACAA TTAAACATAG AAAAAAGAAA TTTATTTGAT CTATGTATCA CATTTCTTCC TTCTGCTATC ACTTATATAA AAGGCGCTAA TGGTTCTGGA AAAAGTTCGC TATTGCGTAT GATAGCCGGG ATTATGCAAC CAAGTAGCGG TAATATCTAC TATAAAAATA GTCATATCAA CAATTGCCAA AAACCTTACT GCACGTATAT AGGTCATAAT TTAGGTATAA AACTCGAAAT GACTGTTTTT GAAAATCTTA AATTTTGGTC AGAAATTTAT AATTCTACTG AAACCGTACA AGCTGCCATT CATTACTTCA AACTAGACTA TTTACTAGAT AAAAAATGCT ATAACTTATC TAGCGGGATA CAGAAAGTTG TAGCTATAGC CAGGCTGATT GTTTGCAAAT CAGATTTATG GTTACTTGAT GAAGTTGAAA CTAACTTAAG CAAAGAAAAT AGAGATTTAC TAAATAATTT AATTATCATG AAAGCAAATA GCGGTGGTAT TATTCTTCTA TCATCACATC AAGAAAGTGT TATAAAATCT GCACAGATAT TACAGCTTAA G

Details for RityA.10014.b.B1.GE35378

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS MLSLHQLQLN IEKRNLFDLC ITFLPSAITY IKGANGSGKS SLLRMIAGIM QPSSGNIYYK NSHINNCQKP YCTYIGHNLG IKLEMTVFEN LKFWSEIYNS TETVQAAIHY FKLDYLLDKK CYNLSSGIQK VVAIARLIVC KSDLWLLDEV ETNLSKENRD LLNNLIIMKA NSGGIILLSS HQESVIKSAQ ILQLK

Validated NT Sequence

ttgtnnnntt taagaaggag atataccatg gctcaccacc accaccacca tatgagtatg ttatcacttc atcaattaca attaaacata gaaaaaagaa atttatttga tctatgtatc acatttcttc cttctgctat cacttatata aaaggcgcta atggttctgg aaaaagttcg ctattgcgta tgatagccgg gattatgcaa ccaagtagcg gtaatatcta ctataaaaat agtcatatca acaattgcca aaaaccttac tgcacgtata taggtcataa tttaggtata aaactcgaaa tgactgtttt tgaaaatctt aaattttggt cagaaattta taattctact gaaaccgtac aagctgccat tcattacttc aaactagact atttactaga taaaaaatgc tataacttat ctagcgggat acagaaagtt gtagctatag ccaggctgat tgtttgcaaa tcagatttat ggttacttga tgaagttgaa actaacttaa gcaaagaaaa tagagattta ctaaataatt taattatcat gaaagcaaat agcggtggta ttattcttct atcatcacat caagaaagtg ttataaaatc tgcacagata ttacagctta agtaagtgag taagatagga tccggctgct aacna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta tgttatcact tcatcaatta caattaaaca tagaaaaaag aaatttattt gatctatgta tcacatttct tccttctgct atcacttata taaaaggcgc taatggttct ggaaaaagtt cgctattgcg tatgatagcc gggattatgc aaccaagtag cggtaatatc tactataaaa atagtcatat caacaattgc caaaaacctt actgcacgta tataggtcat aatttaggta taaaactcga aatgactgtt tttgaaaatc ttaaattttg gtcagaaatt tataattcta ctgaaaccgt acaagctgcc attcattact tcaaactaga ctatttacta gataaaaaat gctataactt atctagcggg atacagaaag ttgtagctat agccaggctg attgtttgca aatcagattt atggttactt gatgaagttg aaactaactt aagcaaagaa aatagagatt tactaaataa tttaattatc atgaaagcaa atagcggtgg tattattctt ctatcatcac atcaagaaag tgttataaaa tctgcacaga tattacagct taagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat