SSGCID: Target Page

RityA.01649.a

Hemolysin C

CENTER ID:	RityA.01649.a
ORGANISM:	Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE:	Murine typhus (endemic typhus)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.01649.a.B1.GE35385	Full length( RityA.01649.a )		1	305

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|257363.4.peg.745
RefSeq:	YP_067664.1
UniProt:	Q68W10

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKSSKKEDS SSKKNQDNKL IFIVRQLFYL IKHLFSKTKT PDNFFGIIKR LKINSKKMSL DECNILANLL QLENKTVEDI MVPRSDIVAI KLTTNLAELS ESIKIEVPHT RTLIYDGTLD NVVGFIHIKD LFKALATKQN STLKRLIRKH IIAAPSMKLL DLLAKMRRER THIAIVVDEY GGTDGLVTIE DLIEEIVGRI DDEHDQQLDS TNFKVINNST IIANARIEVE LLEEIIKEKI KNDDDEFDTI GGLVLTRVSS VPAIGTRIDI SENIEIEVID ATPRSLKQVK IRLKNGLNRD SFNLT

NT Sequence

ATGTTAAAAT CTTCAAAAAA AGAAGATTCT AGTAGTAAAA AAAATCAAGA TAATAAATTA ATTTTTATTG TACGACAATT ATTCTATCTG ATAAAACATC TTTTTAGCAA AACAAAAACG CCTGATAATT TTTTTGGTAT TATAAAGCGT CTTAAAATTA ATAGCAAAAA AATGTCTTTA GATGAGTGTA ATATTTTAGC TAATTTATTG CAGTTGGAAA ATAAAACTGT TGAAGATATA ATGGTACCGC GTTCTGATAT TGTCGCAATA AAATTAACTA CGAATCTAGC AGAATTAAGT GAGTCTATTA AGATAGAAGT CCCACATACA CGAACACTTA TATATGACGG CACTTTGGAC AATGTAGTAG GGTTCATTCA TATAAAGGAT TTATTTAAAG CATTAGCTAC AAAGCAAAAT AGTACTTTAA AAAGACTTAT ACGTAAACAT ATAATTGCAG CTCCTTCTAT GAAATTATTG GATTTGTTAG CAAAAATGCG TCGTGAGAGA ACACATATTG CAATAGTTGT AGATGAGTAT GGTGGTACTG ATGGTTTAGT TACTATTGAA GATCTAATAG AAGAAATAGT AGGGCGAATA GATGATGAAC ATGATCAGCA GTTAGATAGC ACTAATTTCA AAGTTATTAA TAATTCAACA ATTATTGCGA ATGCGCGTAT TGAAGTAGAA TTGCTTGAAG AAATAATAAA AGAAAAGATA AAAAATGATG ATGATGAGTT TGATACAATA GGTGGACTTG TGTTGACTAG GGTTAGTAGT GTTCCAGCTA TTGGTACTAG AATAGATATT TCAGAAAATA TTGAAATTGA AGTGATAGAT GCAACTCCTC GTTCCTTAAA ACAAGTCAAA ATTAGATTAA AAAACGGTTT GAATCGTGAT AGTTTTAATT TAACA

Details for RityA.01649.a.B1.GE35385

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML KSSKKEDSSS KKNQDNKLIF IVRQLFYLIK HLFSKTKTPD NFFGIIKRLK INSKKMSLDE CNILANLLQL ENKTVEDIMV PRSDIVAIKL TTNLAELSES IKIEVPHTRT LIYDGTLDNV VGFIHIKDLF KALATKQNST LKRLIRKHII AAPSMKLLDL LAKMRRERTH IAIVVDEYGG TDGLVTIEDL IEEIVGRIDD EHDQQLDSTN FKVINNSTII ANARIEVELL EEIIKEKIKN DDDEFDTIGG LVLTRVSSVP AIGTRIDISE NIEIEVIDAT PRSLKQVKIR LKNGLNRDSF NLT

Validated NT Sequence

tactcactta tgttaaatta aaactatcac gattcaaacc gttttttaat ctaattttga cttgttttaa ggaacgagga gttgcatcta tcacttcaat ttcaatattt tctgaaatat ctattctagt accaatagct ggaacactac taaccctagt caacacaagt ccacctattg tatcaaactc atcatcatca ttttttatct tttcttttat tatttcttca agcaattcta cttcaatacg cgcattcgca ataattgttg aattattaat aactttgaaa ttagtgctat ctaactgctg atcatgttca tcatctattc gccctactat ttcttctatt agatcttcaa tagtaactaa accatcagta ccaccatact catctacaac tattgcaata tgtgttctct cacgacgcat ttttgctaac aaatccaata atttcataga aggagctgca attatatgtt tacgtataag tctttttaaa gtactatttt gctttgtagc taatgcttta aataaatcct ttatatgaat gaaccctact acattgtcca aagtgccgtc atatataagt gttcgtgtat gtgggacttc tatcttaata gactcactta attctgctag attcgtagtt aattttattg cgacaatatc agaacgcggt accattatat cttcaacagt tttattttcc aactgcaata aattagctaa aatattacac tcatctaaag acattttttt gctattaatt ttaagacgct ttataatacc aaaaaaatta tcaggcgttt ttgttttgct aaaaagatgt tttatcagat agaataattg tcgtacaata aaaattaatt tattatcttg atttttttta ctactagaat cttctttttt tgaagatttt aacatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa aatcttcaaa aaaagaagat tctagtagta aaaaaaatca agataataaa ttaattttta ttgtacgaca attattctat ctgataaaac atctttttag caaaacaaaa acgcctgata atttttttgg tattataaag cgtcttaaaa ttaatagcaa aaaaatgtct ttagatgagt gtaatatttt agctaattta ttgcagttgg aaaataaaac tgttgaagat ataatggtac cgcgttctga tattgtcgca ataaaattaa ctacgaatct agcagaatta agtgagtcta ttaagataga agtcccacat acacgaacac ttatatatga cggcactttg gacaatgtag tagggttcat tcatataaag gatttattta aagcattagc tacaaagcaa aatagtactt taaaaagact tatacgtaaa catataattg cagctccttc tatgaaatta ttggatttgt tagcaaaaat gcgtcgtgag agaacacata ttgcaatagt tgtagatgag tatggtggta ctgatggttt agttactatt gaagatctaa tagaagaaat agtagggcga atagatgatg aacatgatca gcagttagat agcactaatt tcaaagttat taataattca acaattattg cgaatgcgcg tattgaagta gaattgcttg aagaaataat aaaagaaaag ataaaaaatg atgatgatga gtttgataca ataggtggac ttgtgttgac tagggttagt agtgttccag ctattggtac tagaatagat atttcagaaa atattgaaat tgaagtgata gatgcaactc ctcgttcctt aaaacaagtc aaaattagat taaaaaacgg tttgaatcgt gatagtttta atttaacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat