RityA.00893.a

Thioredoxin reductase

CENTER ID: RityA.00893.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.00893.a.B2.GE35375 Mature protein( RityA.00893.a ) 19 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|257363.4.peg.461
RefSeq: YP_067391.1
UniProt: Q68WT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKITTKVLII GSGPAGLSAA IYTARSSLKP ILINGMQPGG QLTMTTDVEN YPGFAKTIQG PWLMEQMSIQ AKNVGTEIIN DYVERVDLSK RPFKIFTGTG NKYEADSIII CTGAESKWLG ITSEQEFRGF GVSSCAICDG FFFKNQDIVV VGGGNSALEE ALYLTNHANK VTVVHRRNSF RAEKILQDRL FKNPKISVIW DHVIDEIVGS NQPKTVTGVK IKNVYTNEIN LVNCSGVFIA IGHTPNTTLF NGQIAIDDDN YIITQTGSTR TSVEGVFAAG DVQDKIYRQA ITAAASGCMA ALEVAKFLNK
NT Sequence
ATGAAAATTA CTACTAAAGT GCTAATAATA GGTTCAGGTC CTGCTGGCTT AAGTGCCGCT ATTTATACAG CTAGATCATC TCTAAAACCA ATATTAATTA ATGGTATGCA GCCAGGTGGG CAGCTTACAA TGACAACTGA CGTTGAAAAT TATCCTGGAT TTGCAAAAAC CATACAAGGG CCTTGGCTTA TGGAGCAGAT GTCTATACAG GCTAAAAATG TCGGTACAGA AATTATCAAT GATTACGTAG AAAGAGTAGA TTTATCAAAG AGACCATTTA AAATATTTAC TGGAACTGGG AATAAGTATG AAGCAGATAG TATAATTATT TGTACTGGTG CGGAGTCAAA ATGGCTTGGT ATAACTTCGG AACAAGAATT TCGTGGTTTT GGCGTTTCAT CATGTGCTAT TTGTGATGGT TTTTTTTTCA AGAATCAAGA CATTGTTGTA GTAGGTGGCG GAAATAGTGC ATTAGAAGAA GCTTTATATT TAACCAATCA TGCTAATAAA GTTACTGTAG TGCATAGAAG AAATAGTTTT AGAGCTGAAA AAATATTACA AGATCGATTA TTTAAAAATC CTAAAATATC AGTAATTTGG GATCATGTGA TAGATGAGAT TGTAGGTAGT AATCAACCAA AAACTGTTAC TGGCGTGAAA ATTAAAAATG TTTATACTAA CGAGATTAAC TTAGTGAATT GTAGTGGTGT ATTTATAGCT ATCGGGCATA CACCAAATAC CACTCTATTT AATGGGCAAA TTGCAATAGA TGACGATAAT TATATTATCA CACAAACAGG CTCGACGAGA ACTAGTGTAG AAGGTGTTTT TGCTGCCGGC GACGTTCAGG ATAAAATTTA TAGACAAGCA ATTACTGCTG CAGCTAGCGG TTGTATGGCA GCTCTTGAAG TAGCAAAATT TCTAAATAAA
Details for RityA.00893.a.B2.GE35375
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA AIYTARSSLK PILINGMQPG GQLTMTTDVE NYPGFAKTIQ GPWLMEQMSI QAKNVGTEII NDYVERVDLS KRPFKIFTGT GNKYEADSII ICTGAESKWL GITSEQEFRG FGVSSCAICD GFFFKNQDIV VVGGGNSALE EALYLTNHAN KVTVVHRRNS FRAEKILQDR LFKNPKISVI WDHVIDEIVG SNQPKTVTGV KIKNVYTNEI NLVNCSGVFI AIGHTPNTTL FNGQIAIDDD NYIITQTGST RTSVEXVFAA GDVQDKIYRQ AITAAASGCM AALEVAKFLN K
Validated NT Sequence
aanaattttg tttancttta agaaggagan ataccatggc tcaccaccac caccaccata tggccgctat ttatacagct agatcatctc taaaaccaat attaattaat ggtatgcagc caggtgggca gcttacaatg acaactgacg ttgaaaatta tcctggattt gcaaaaacca tacaagggcc ttggcttatg gagcagatgt ctatacaggc taaaaatgtc ggtacagaaa ttatcaatga ttacgtagaa agagtagatt tatcaaagag accatttaaa atatttactg gaactgggaa taagtatgaa gcagatagta taattatttg tactggtgcg gagtcaaaat ggcttggtat aacttcggaa caagaatttc gtggttttgg cgtttcatca tgtgctattt gtgatggttt ttttttcaag aatcaagaca ttgttgtagt aggtggcgga aatagtgcat tagaagaagc tttatattta accaatcatg ctaataaagt tactgtagtg catagaagaa atagttttag agctgaaaaa atattacaag atcgattatt taaaaatcct aaaatatcag taatttggga tcatgtgata gatgagattg taggtagtaa tcaaccaaaa actgttactg gcgtgaaaat taaaaatgtt tatactaacg agattaactt agtgaattgt agtggtgtat ttatagctat cgggcataca ccaaatacca ctctatttaa tgggcaaatt gcaatagatg acgataatta tattatcaca caaacaggct cgacgagaac tagtgtagaa ngtgtttttg ctgccggcga cgttcaggat aaaatttata gacaagcaat tactgctgca gctagcggtt gtatggcagc tcttgaagta gcaaaatttc taaataaata agtgagtaan atagganccg gctgctannn nnncccgana
Expected Protein Sequence
MAHHHHHHMA AIYTARSSLK PILINGMQPG GQLTMTTDVE NYPGFAKTIQ GPWLMEQMSI QAKNVGTEII NDYVERVDLS KRPFKIFTGT GNKYEADSII ICTGAESKWL GITSEQEFRG FGVSSCAICD GFFFKNQDIV VVGGGNSALE EALYLTNHAN KVTVVHRRNS FRAEKILQDR LFKNPKISVI WDHVIDEIVG SNQPKTVTGV KIKNVYTNEI NLVNCSGVFI AIGHTPNTTL FNGQIAIDDD NYIITQTGST RTSVEGVFAA GDVQDKIYRQ AITAAASGCM AALEVAKFLN K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg ctatttatac agctagatca tctctaaaac caatattaat taatggtatg cagccaggtg ggcagcttac aatgacaact gacgttgaaa attatcctgg atttgcaaaa accatacaag ggccttggct tatggagcag atgtctatac aggctaaaaa tgtcggtaca gaaattatca atgattacgt agaaagagta gatttatcaa agagaccatt taaaatattt actggaactg ggaataagta tgaagcagat agtataatta tttgtactgg tgcggagtca aaatggcttg gtataacttc ggaacaagaa tttcgtggtt ttggcgtttc atcatgtgct atttgtgatg gttttttttt caagaatcaa gacattgttg tagtaggtgg cggaaatagt gcattagaag aagctttata tttaaccaat catgctaata aagttactgt agtgcataga agaaatagtt ttagagctga aaaaatatta caagatcgat tatttaaaaa tcctaaaata tcagtaattt gggatcatgt gatagatgag attgtaggta gtaatcaacc aaaaactgtt actggcgtga aaattaaaaa tgtttatact aacgagatta acttagtgaa ttgtagtggt gtatttatag ctatcgggca tacaccaaat accactctat ttaatgggca aattgcaata gatgacgata attatattat cacacaaaca ggctcgacga gaactagtgt agaaggtgtt tttgctgccg gcgacgttca ggataaaatt tatagacaag caattactgc tgcagctagc ggttgtatgg cagctcttga agtagcaaaa tttctaaata aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat