RityA.00169.a

Replicative DNA helicase

CENTER ID: RityA.00169.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.00169.a.B1.GE35408 Full length( RityA.00169.a ) 1 497
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|257363.4.peg.555
RefSeq: YP_067482.1
UniProt: Q68WJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVRNKINNNI NIITNNDDNL SIPRVLPSNI QAEQMLLGAI ITNNELLSYV SEFLRNEHFF EPIHQKIYDA IEKIIEKGLI ATPITLRSML TQDALFQEIE GVEYLAKLIT MSMMVINPID YGKIIYDLAI KRNLINIGEE VVNNAYNASL AVAAKEQIEH AEAKLYDLAR EGLNEKSFTQ VGISIAESLA SINKAMKNND HVIGISTGLL DLDNKLFGFH NSDLIILAGR PSMGKTAFAI NLALNTCNNM RLKNIRDNQE IKSVGFFSLE MSSEQLTTRL LSLCAEIDST SLRTGMLSEE KYNRLRKEAN TLSELQFFID DTPALSISAI RTRARRMKRK HNLGILFIDY LQLIRGVSKS ENRVNEISEI TQGLKAIAKE LNIPVIALSQ LSRAVELRED KKPMLSDLRE SGTIEQDADI VMFIYREEYY LTRKEPAAGD AKHAEWLDKL NKVYNIADII IAKHRNGPVG NISLYYDSQF SKFGNLEKRT FNSILNT
NT Sequence
ATGGTACGTA ATAAAATAAA TAACAACATA AATATAATCA CTAATAACGA TGATAATTTA TCAATACCAA GAGTTTTACC TTCAAATATT CAGGCAGAAC AAATGCTGTT AGGTGCAATT ATAACTAATA ATGAATTACT CAGTTATGTA TCAGAATTTT TACGTAATGA ACATTTCTTT GAACCTATTC ATCAAAAAAT CTATGATGCA ATTGAAAAAA TTATCGAAAA AGGTTTGATA GCTACACCAA TTACTTTACG TAGTATGCTA ACTCAAGATG CACTATTCCA AGAAATAGAG GGAGTGGAAT ATTTAGCAAA ATTGATAACT ATGTCAATGA TGGTGATAAA TCCTATTGAT TACGGTAAAA TAATATATGA TTTAGCGATA AAGCGTAATT TAATAAATAT AGGTGAAGAA GTAGTAAATA ATGCCTATAA TGCTTCATTA GCAGTTGCAG CAAAAGAACA GATTGAACAT GCTGAAGCTA AACTTTACGA TTTAGCGCGA GAAGGCTTGA ATGAGAAAAG TTTTACCCAA GTCGGTATAT CTATTGCAGA ATCACTTGCT AGTATTAATA AGGCCATGAA AAATAATGAT CACGTAATCG GTATATCTAC CGGTTTGCTT GATCTAGATA ATAAATTATT CGGTTTTCAT AATTCTGATC TTATAATTCT TGCCGGACGC CCGTCCATGG GTAAAACTGC ATTTGCTATA AATCTTGCGC TTAACACCTG TAATAATATG CGTCTTAAAA ATATTAGGGA TAATCAAGAA ATTAAGTCAG TAGGTTTTTT TTCTTTAGAA ATGTCTTCAG AACAACTAAC TACACGTCTA CTTTCACTTT GTGCAGAAAT TGATTCTACT TCTCTTCGTA CTGGGATGCT AAGTGAAGAG AAATATAATC GTCTTCGCAA AGAAGCAAAT ACTTTATCGG AACTACAATT TTTTATTGAT GATACACCTG CTTTATCTAT TTCTGCTATA AGAACACGAG CTAGAAGGAT GAAACGCAAG CATAATCTTG GTATATTATT TATTGATTAT TTACAATTAA TTAGAGGCGT TAGTAAATCT GAAAATAGAG TTAATGAGAT TTCAGAAATC ACTCAAGGTT TAAAAGCAAT AGCAAAAGAG TTAAATATTC CGGTTATTGC GTTATCTCAG CTTTCAAGAG CAGTAGAATT ACGTGAAGAT AAAAAACCTA TGCTATCGGA TCTTCGAGAA TCTGGCACTA TAGAACAGGA TGCAGATATA GTAATGTTTA TTTACCGTGA AGAATATTAT TTAACTAGAA AAGAACCGGC AGCAGGCGAT GCCAAACACG CTGAATGGTT AGATAAACTG AATAAAGTAT ATAATATTGC AGACATAATA ATTGCAAAAC ATCGTAACGG CCCAGTTGGC AATATTTCTC TTTATTATGA TAGTCAATTT TCTAAATTTG GAAATTTAGA GAAAAGAACT TTTAACTCTA TATTAAATAC T
Details for RityA.00169.a.B1.GE35408
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMV RNKINNNINI ITNNDDNLSI PRVLPSNIQA EQMLLGAIIT NNELLSYVSE FLRNEHFFEP IHQKIYDAIE KIIEKGLIAT PITLRSMLTQ DALFQEIEGV EYLAKLITMS MMVINPIDYG KIIYDLAIKR NLINIGEEVV NNAYNASLAV AAKEQIEHAE AKLYDLAREG LNEKSFTQVG ISIAESLASI NKAMKNNDHV IGISTGLLDL DNKLFGFHNS DLIILAGRPS MGKTAFAINL ALNTCNNMRL KNIRDNQEIK SVGFFSLEMS SEQLTTRLLS LCAEIXSTSL RTGMLSEEXY NRLRKEANTL SXLXFFXDXX XALSXXAXXT XXXXXXQXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
ttgtttaact ttaagaagga gatataccat ggctcaccac caccaccacc atatggtacg taataaaata aataacaaca taaatataat cactaataac gatgataatt tatcaatacc aagagtttta ccttcaaata ttcaggcaga acaaatgctg ttaggtgcaa ttataactaa taatgaatta ctcagttatg tatcagaatt tttacgtaat gaacatttct ttgaacctat tcatcaaaaa atctatgatg caattgaaaa aattatcgaa aaaggtttga tagctacacc aattacttta cgtagtatgc taactcaaga tgcactattc caagaaatag agggagtgga atatttagca aaattgataa ctatgtcaat gatggtgata aatcctattg attacggtaa aataatatat gatttagcga taaagcgtaa tttaataaat ataggtgaag aagtagtaaa taatgcctat aatgcttcat tagcagttgc agcaaaagaa cagattgaac atgctgaagc taaactttac gatttagcgc gagaaggctt gaatgagaaa agttttaccc aagtcggtat atctattgca gaatcacttg ctagtattaa taaggccatg aaaaataatg atcacgtaat cggtatatct accggtttgc ttgatctaga taataaatta ttcggttttc ataattctga tcttataatt cttgccggac gcccgtccat gggtaaaact gcatttgcta taaatcttgc gcttaacacc tgtaataata tgcgtcttaa aaatattagg gataatcaag aaattaagtc agtaggtttt ttttctttag aaatgtcttc agaacaacta actacacgtc tactttcact ttgtgcagaa attgantcta cttctcttcg tactgggatg ctaagtgaag agaantataa tcgtcttcgc aaagaagcna atactttatc nnaactnnna ttttttantg atgannnnnc ngctttatcn nntnctgctn nnanaacncn anctannnnn naannncagc ntnannntng nnnntnantt nnnngntnnt tnnnnnnnnn nnnntnnnan nntgnaaann ngantnnnnn attnnnaann nnnnnnnntn naancnnntn nnnnnntnnn nnnnnnnnnn nnnnnnnnnn nnannnnaa
Expected Protein Sequence
MAHHHHHHMV RNKINNNINI ITNNDDNLSI PRVLPSNIQA EQMLLGAIIT NNELLSYVSE FLRNEHFFEP IHQKIYDAIE KIIEKGLIAT PITLRSMLTQ DALFQEIEGV EYLAKLITMS MMVINPIDYG KIIYDLAIKR NLINIGEEVV NNAYNASLAV AAKEQIEHAE AKLYDLAREG LNEKSFTQVG ISIAESLASI NKAMKNNDHV IGISTGLLDL DNKLFGFHNS DLIILAGRPS MGKTAFAINL ALNTCNNMRL KNIRDNQEIK SVGFFSLEMS SEQLTTRLLS LCAEIDSTSL RTGMLSEEKY NRLRKEANTL SELQFFIDDT PALSISAIRT RARRMKRKHN LGILFIDYLQ LIRGVSKSEN RVNEISEITQ GLKAIAKELN IPVIALSQLS RAVELREDKK PMLSDLRESG TIEQDADIVM FIYREEYYLT RKEPAAGDAK HAEWLDKLNK VYNIADIIIA KHRNGPVGNI SLYYDSQFSK FGNLEKRTFN SILNT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtac gtaataaaat aaataacaac ataaatataa tcactaataa cgatgataat ttatcaatac caagagtttt accttcaaat attcaggcag aacaaatgct gttaggtgca attataacta ataatgaatt actcagttat gtatcagaat ttttacgtaa tgaacatttc tttgaaccta ttcatcaaaa aatctatgat gcaattgaaa aaattatcga aaaaggtttg atagctacac caattacttt acgtagtatg ctaactcaag atgcactatt ccaagaaata gagggagtgg aatatttagc aaaattgata actatgtcaa tgatggtgat aaatcctatt gattacggta aaataatata tgatttagcg ataaagcgta atttaataaa tataggtgaa gaagtagtaa ataatgccta taatgcttca ttagcagttg cagcaaaaga acagattgaa catgctgaag ctaaacttta cgatttagcg cgagaaggct tgaatgagaa aagttttacc caagtcggta tatctattgc agaatcactt gctagtatta ataaggccat gaaaaataat gatcacgtaa tcggtatatc taccggtttg cttgatctag ataataaatt attcggtttt cataattctg atcttataat tcttgccgga cgcccgtcca tgggtaaaac tgcatttgct ataaatcttg cgcttaacac ctgtaataat atgcgtctta aaaatattag ggataatcaa gaaattaagt cagtaggttt tttttcttta gaaatgtctt cagaacaact aactacacgt ctactttcac tttgtgcaga aattgattct acttctcttc gtactgggat gctaagtgaa gagaaatata atcgtcttcg caaagaagca aatactttat cggaactaca attttttatt gatgatacac ctgctttatc tatttctgct ataagaacac gagctagaag gatgaaacgc aagcataatc ttggtatatt atttattgat tatttacaat taattagagg cgttagtaaa tctgaaaata gagttaatga gatttcagaa atcactcaag gtttaaaagc aatagcaaaa gagttaaata ttccggttat tgcgttatct cagctttcaa gagcagtaga attacgtgaa gataaaaaac ctatgctatc ggatcttcga gaatctggca ctatagaaca ggatgcagat atagtaatgt ttatttaccg tgaagaatat tatttaacta gaaaagaacc ggcagcaggc gatgccaaac acgctgaatg gttagataaa ctgaataaag tatataatat tgcagacata ataattgcaa aacatcgtaa cggcccagtt ggcaatattt ctctttatta tgatagtcaa ttttctaaat ttggaaattt agagaaaaga acttttaact ctatattaaa tacttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat