SSGCID: Target Page

RityA.00166.a

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase

CENTER ID:	RityA.00166.a
ORGANISM:	Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE:	Murine typhus (endemic typhus)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.00166.a.B1.GE35400	Full length( RityA.00166.a )		1	289

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|257363.4.peg.254
RefSeq:	YP_067209.1
UniProt:	Q68XB5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQQSTLLKPV SCYGIGVHTG KRTQLTIEPA KENTGIIFIR TDISSENNYI EASYYNVSDT LLSTTISNNH KIQVSTIEHL MAALWGCSID NAVIKIDGPE VPIMDGSSKP FVFMIECAGK KLQNAPKKYL KILKEIKVVN KDCELYCTPS EHITVDLTID FSSKAIGKQN LNFGVQESFT KNIADARTFG FIKDVEYLKS KGLAQGASFE NAIGIDEHDK VLNPSGLRYA DEFVRHKLLD LFGDLYTSGI SIVSAIKGYK TSHAFNNELL HKIFSDTTSY KFVTSNELS

NT Sequence

ATGCAACAAA GTACATTATT AAAACCAGTT AGTTGTTACG GTATTGGGGT GCACACAGGA AAACGCACAC AGTTAACCAT AGAACCCGCT AAAGAGAATA CTGGAATTAT ATTCATTAGA ACTGATATTT CTTCTGAAAA TAATTATATT GAAGCTAGCT ATTATAATGT ATCCGATACT TTGTTATCGA CGACTATAAG TAATAATCAT AAAATTCAAG TTTCAACAAT TGAACATTTA ATGGCGGCAC TTTGGGGATG CAGCATTGAT AATGCAGTCA TTAAAATTGA TGGTCCTGAA GTGCCAATTA TGGACGGTAG TAGTAAACCT TTTGTGTTTA TGATTGAATG TGCAGGTAAA AAATTACAAA ATGCTCCTAA AAAATATCTA AAAATTTTAA AAGAGATTAA AGTAGTTAAT AAAGACTGTG AATTATATTG TACCCCTTCC GAACACATTA CTGTAGATTT AACTATTGAT TTTAGTAGTA AAGCTATAGG GAAGCAAAAT CTAAATTTTG GAGTTCAAGA ATCATTTACT AAAAATATTG CTGATGCTAG AACTTTTGGT TTTATAAAAG ATGTTGAGTA TCTGAAAAGC AAAGGACTCG CACAAGGTGC CTCATTTGAG AATGCTATAG GAATAGACGA GCATGATAAA GTATTAAACC CAAGTGGCTT ACGTTATGCA GATGAGTTTG TGCGTCATAA ACTATTAGAT TTATTCGGCG ATTTATATAC AAGCGGCATT AGCATTGTAA GTGCAATTAA AGGGTATAAA ACCAGCCATG CTTTTAATAA CGAATTATTG CATAAAATAT TTAGCGATAC TACATCTTAT AAATTTGTAA CCAGTAACGA GTTATCA

Details for RityA.00166.a.B1.GE35400

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMQ QSTLLKPVSC YGIGVHTGKR TQLTIEPAKE NTGIIFIRTD ISSENNYIEA SYYNVSDTLL STTISNNHKI QVSTIEHLMA ALWGCSIDNA VIKIDGPEVP IMDGSSKPFV FMIECAGKKL QNAPKKYLKI LKEIKVVNKD CELYCTPSEH ITVDLTIDFS SKAIGKQNLN FGVQESFTKN IADARTFGFI KDVEYLKSKG LAQGASFENA IGIDEHDKVL NPSGLRYADE FVRHKLLDLF GDLYTSGISI VSAIKGYKTS HAFNNELLHK IFSDTTSYKF VTXNELS

Validated NT Sequence

ttgttnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgcaacaa agtacattat taaaaccagt tagttgttac ggtattgggg tgcacacagg aaaacgcaca cagttaacca tagaacccgc taaagagaat actggaatta tattcattag aactgatatt tcttctgaaa ataattatat tgaagctagc tattataatg tatccgatac tttgttatcg acgactataa gtaataatca taaaattcaa gtttcaacaa ttgaacattt aatggcggca ctttggggat gcagcattga taatgcagtc attaaaattg atggtcctga agtgccaatt atggacggta gtagtaaacc ttttgtgttt atgattgaat gtgcaggtaa aaaattacaa aatgctccta aaaaatatct aaaaatttta aaagagatta aagtagttaa taaagactgt gaattatatt gtaccccttc cgaacacatt actgtagatt taactattga ttttagtagt aaagctatag ggaagcaaaa tctaaatttt ggagttcaag aatcatttac taaaaatatt gctgatgcta gaacttttgg ttttataaaa gatgttgagt atctgaaaag caaaggactc gcacaaggtg cctcatttga gaatgctata ggaatagacg agcatgataa agtattaaac ccaagtggct tacgttatgc agatgagttt gtgcgtcata aactattaga tttattcggc gatttatata caagcggcat tagcattgta agtgcaatta aagggtataa aaccagccat gcttttaata acgaattatt gcataaaata tttagcgata ctacatctta taaatttgta accngtaacg agttatcata agtgagtaan ataggatccg gctgcnannn nnncc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac aaagtacatt attaaaacca gttagttgtt acggtattgg ggtgcacaca ggaaaacgca cacagttaac catagaaccc gctaaagaga atactggaat tatattcatt agaactgata tttcttctga aaataattat attgaagcta gctattataa tgtatccgat actttgttat cgacgactat aagtaataat cataaaattc aagtttcaac aattgaacat ttaatggcgg cactttgggg atgcagcatt gataatgcag tcattaaaat tgatggtcct gaagtgccaa ttatggacgg tagtagtaaa ccttttgtgt ttatgattga atgtgcaggt aaaaaattac aaaatgctcc taaaaaatat ctaaaaattt taaaagagat taaagtagtt aataaagact gtgaattata ttgtacccct tccgaacaca ttactgtaga tttaactatt gattttagta gtaaagctat agggaagcaa aatctaaatt ttggagttca agaatcattt actaaaaata ttgctgatgc tagaactttt ggttttataa aagatgttga gtatctgaaa agcaaaggac tcgcacaagg tgcctcattt gagaatgcta taggaataga cgagcatgat aaagtattaa acccaagtgg cttacgttat gcagatgagt ttgtgcgtca taaactatta gatttattcg gcgatttata tacaagcggc attagcattg taagtgcaat taaagggtat aaaaccagcc atgcttttaa taacgaatta ttgcataaaa tatttagcga tactacatct tataaatttg taaccagtaa cgagttatca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat