RityA.00157.a

DNA topoisomerase 4 subunit A

CENTER ID: RityA.00157.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.00157.a.B1.GE35402 Full length( RityA.00157.a ) 1 737
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.00157.a.B1.PS01631 Full length( RityA.00157.a ) 1 737

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|257363.4.peg.65
RefSeq: YP_067033.1
UniProt: Q68XU1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKEAKIENID FGNALSERYL AYALSTIMSR SLPDVRDGLK PVHRRLLYAM LQLRLEPNSG YKKCARVVGD VIGKYHPHGD VAVYDTLVRL AQHFSLRYPL IDGQGNFGSI DGDNAAAMRY TESRMTEICM LLMKDIDKDT VDFRSTYDDS DLEPVIMPAS FPNLLANGSE GIAVGMATNI PPHNLHELCD ALLYLIDNPQ AGVNDIMNFI KGPDFPTGGI IIDQTKVINA AYTTGRGSFR VRSRWEKEEL SYGTYQIVVT EIPYQVQKSK LIEQIAILLK DKKIPLISSI RDESTDIIRL VIEPRDRSCD PQIVMESLFK LTNLESRIQL NMNVIGSNNV PRVMNIVEIL QEFLVHRQNI ITRRSTYLLN KIKQRLEILK VLKIVYLNLD EIIEIIRRED EPKTIIMERF KISEIQVEVI LNTRLRALQK LEEHAIIDEH SNLQKQQAIL EKILKNHKEL WKIVKKEIKS VQTQFGLNTI IGARRTSFEE VDLTNQVIDI TAFITKEPIT IICSKMGWIR LLKGHNTDLS TIKYKEGDAE KFIIEAYTTD KILIISSKGR FFTLLADNIS KGKGTAVSIK LLVDIGNNDI TNILVYKPYQ LLLLASSIGK GFLVNSNEVI AQTKAGKQIM NIPEGYACIT CLPVNGNSIA CINESRRLLI FNINEIPEMK KGQGVVLQRF KNAKLLDIKI FNKEDGLSWN DGKKIQLEKN IVAFLGKRGS AGTLPPIGFP KNNRFSS
NT Sequence
ATGAAAGAAG CTAAAATAGA AAATATTGAC TTTGGTAATG CTTTATCAGA GCGTTATCTT GCTTATGCAT TATCGACTAT TATGTCACGT TCACTTCCTG ACGTACGGGA CGGACTCAAA CCTGTACATC GTAGGTTATT ATACGCAATG TTACAACTAA GGCTTGAGCC TAATTCTGGA TATAAGAAAT GTGCAAGAGT GGTTGGTGAC GTAATAGGTA AATACCACCC GCACGGTGAT GTTGCAGTAT ATGATACCTT GGTACGACTT GCACAGCATT TTTCATTGCG TTATCCTTTG ATTGACGGTC AGGGTAATTT TGGCTCTATA GATGGTGATA ATGCAGCAGC TATGCGCTAT ACTGAATCAC GTATGACGGA AATATGCATG TTATTAATGA AGGACATTGA TAAAGATACG GTAGACTTCC GTTCTACTTA TGATGATTCT GATTTAGAGC CGGTAATAAT GCCGGCAAGT TTTCCTAATT TGCTTGCTAA CGGCTCTGAA GGTATAGCAG TTGGTATGGC AACAAATATA CCACCGCATA ATTTACATGA GTTATGTGAT GCTTTATTGT ATTTAATCGA CAATCCGCAA GCTGGGGTTA ACGATATAAT GAACTTTATC AAAGGCCCTG ATTTTCCAAC TGGTGGTATA ATTATTGATC AAACTAAAGT TATTAATGCT GCATATACTA CTGGACGTGG TAGTTTTAGA GTAAGAAGTA GATGGGAGAA AGAGGAACTG AGTTACGGTA CATATCAGAT AGTTGTCACT GAAATACCTT ATCAGGTACA GAAATCAAAA CTTATAGAAC AAATAGCAAT ATTACTAAAA GATAAAAAAA TTCCGCTTAT TAGCAGTATT AGAGATGAAT CAACTGATAT TATAAGGTTG GTTATAGAAC CAAGAGATCG TAGTTGTGAT CCACAGATAG TTATGGAGTC TCTATTTAAG CTCACTAATT TAGAAAGTCG TATTCAACTA AATATGAATG TGATAGGTAG TAATAACGTG CCGAGAGTAA TGAATATTGT AGAGATTCTA CAAGAATTTT TAGTTCATAG ACAAAATATT ATTACTCGTA GGTCAACTTA TCTTTTGAAT AAAATCAAGC AGCGTTTAGA AATTTTAAAA GTGCTTAAAA TAGTTTATTT AAACCTTGAC GAGATAATTG AAATTATCCG TAGAGAGGAT GAGCCAAAAA CAATAATTAT GGAGCGGTTT AAAATAAGTG AAATACAAGT AGAAGTAATA TTAAACACTC GTCTTCGTGC ACTCCAAAAA CTTGAAGAGC ATGCAATTAT TGATGAGCAT AGTAATTTGC AGAAGCAACA AGCTATATTA GAAAAGATTT TAAAAAATCA TAAAGAACTA TGGAAAATAG TGAAAAAAGA AATCAAATCA GTACAAACTC AATTTGGATT AAATACAATA ATAGGAGCAA GGCGTACAAG TTTTGAAGAA GTAGATCTTA CAAATCAAGT TATTGATATA ACTGCGTTCA TTACTAAAGA ACCTATTACT ATTATCTGCT CAAAAATGGG GTGGATACGT TTACTAAAAG GACATAACAC TGATTTATCA ACTATTAAAT ATAAAGAAGG TGATGCGGAA AAGTTTATTA TAGAAGCCTA TACAACTGAT AAAATATTAA TAATTAGTTC AAAAGGAAGA TTTTTTACTT TGCTTGCAGA TAATATTTCT AAAGGTAAAG GTACTGCTGT ATCTATAAAA CTACTTGTGG ATATAGGTAA TAACGACATT ACTAATATTT TAGTCTATAA ACCTTATCAG CTTTTATTAC TTGCAAGTAG TATCGGTAAA GGATTTTTAG TTAATTCAAA CGAAGTAATA GCTCAAACAA AAGCAGGAAA GCAAATTATG AATATACCAG AGGGGTATGC ATGTATAACA TGCTTACCTG TAAATGGCAA TAGTATTGCT TGTATCAATG AAAGTCGTAG ATTACTTATA TTTAATATAA ATGAAATACC TGAAATGAAA AAAGGGCAAG GTGTAGTGCT GCAAAGGTTT AAGAATGCAA AACTTTTAGA TATTAAAATA TTTAATAAAG AAGATGGTTT AAGTTGGAAT GATGGCAAAA AAATACAACT TGAAAAAAAT ATTGTCGCAT TTTTAGGGAA AAGAGGTAGT GCCGGTACAT TGCCACCTAT TGGCTTCCCT AAAAATAATA GATTTTCATC A
Details for RityA.00157.a.B1.GE35402
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 84kDa
OBSERVED MW: 84kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 45
Validated AA Sequence
MAHHHHHHMK EAKIENIDFG NALSERYLAY ALSTIMSRSL PDVRDGLKPV HRRLLYAMLQ LRLEPNSGYK KCARVVGDVI GKYHPHGDVA VYDTLVRLAQ HFSLRYPLID GQGNFGSIDG DNAAAMRYTE SRMTEICMLL MKDIDKDTVD FRSTYDDSDL EPVIMPASFP NLLANGSEGI AVGMATNIPP HNLHELCDAL LYLIDNPQAG VNDIMNFIKG PDFPTGGIII DQTKVINAAY TTGRGSFRVR SRWEKEELSY GTYQIVVTEI PYQXQKSKLI EQIAILLKDK KIPLIXSXRD ESTDIXXXGY RTXRSXLXSX DSYGVXXLSS LXXKVVXX
Validated NT Sequence
cnnnnnnnnn nnttttgttt aactttaaga aggagaatac catggctcac caccaccacc accatatgaa agaagctaaa atagaaaata ttgactttgg taatgcttta tcagagcgtt atcttgctta tgcattatcg actattatgt cacgttcact tcctgacgta cgggacggac tcaaacctgt acatcgtagg ttattatacg caatgttaca actaaggctt gagcctaatt ctggatataa gaaatgtgca agagtggttg gtgacgtaat aggtaaatac cacccgcacg gtgatgttgc agtatatgat accttggtac gacttgcaca gcatttttca ttgcgttatc ctttgattga cggtcagggt aattttggct ctatagatgg tgataatgca gcagctatgc gctatactga atcacgtatg acggaaatat gcatgttatt aatgaaggac attgataaag atacggtaga cttccgttct acttatgatg attctgattt agagccggta ataatgccgg caagttttcc taatttgctt gctaacggct ctgaaggtat agcagttggt atggcaacaa atataccacc gcataattta catgagttat gtgatgcttt attgtattta atcgacaatc cgcaagctgg ggttaacgat ataatgaact ttatcaaagg ccctgatttt ccnactggtg gtataattat tgatcaaact aaagttatta atgctgcata tactactgga cgtggtagtt ttagagtaag aagtagatgg gagaaagagg aactgagtta cggtacatat cagatagttg tcactgaaat accttatcag gnacagaaat caaaacttat agaacaaata gcaatattac taaaagataa aaaaattccg cttattanca gtantagaga tgaatcaact gatattatna ngntgggtta tagaaccnag agatcgtant tgngatccnc agatagttat ggagtcnnnn ntttaagctc nctantttan aaagtcgtnn tcnnctaann nnngaatgnn gatnngtagt antnannnnn cnnnnagtan ngaannnnnn nnnnnatnct ncannntttn nnnnnaannn nnntnctcnn ngnnannnnn nnntttnnnn naaanccnnn nnnnnnnann ntnnnnnnnn nnnnnnnnnn nnnnnncnan nntnntnnnn nnnnngnnnn nnnnncc
Expected Protein Sequence
MAHHHHHHMK EAKIENIDFG NALSERYLAY ALSTIMSRSL PDVRDGLKPV HRRLLYAMLQ LRLEPNSGYK KCARVVGDVI GKYHPHGDVA VYDTLVRLAQ HFSLRYPLID GQGNFGSIDG DNAAAMRYTE SRMTEICMLL MKDIDKDTVD FRSTYDDSDL EPVIMPASFP NLLANGSEGI AVGMATNIPP HNLHELCDAL LYLIDNPQAG VNDIMNFIKG PDFPTGGIII DQTKVINAAY TTGRGSFRVR SRWEKEELSY GTYQIVVTEI PYQVQKSKLI EQIAILLKDK KIPLISSIRD ESTDIIRLVI EPRDRSCDPQ IVMESLFKLT NLESRIQLNM NVIGSNNVPR VMNIVEILQE FLVHRQNIIT RRSTYLLNKI KQRLEILKVL KIVYLNLDEI IEIIRREDEP KTIIMERFKI SEIQVEVILN TRLRALQKLE EHAIIDEHSN LQKQQAILEK ILKNHKELWK IVKKEIKSVQ TQFGLNTIIG ARRTSFEEVD LTNQVIDITA FITKEPITII CSKMGWIRLL KGHNTDLSTI KYKEGDAEKF IIEAYTTDKI LIISSKGRFF TLLADNISKG KGTAVSIKLL VDIGNNDITN ILVYKPYQLL LLASSIGKGF LVNSNEVIAQ TKAGKQIMNI PEGYACITCL PVNGNSIACI NESRRLLIFN INEIPEMKKG QGVVLQRFKN AKLLDIKIFN KEDGLSWNDG KKIQLEKNIV AFLGKRGSAG TLPPIGFPKN NRFSS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aagctaaaat agaaaatatt gactttggta atgctttatc agagcgttat cttgcttatg cattatcgac tattatgtca cgttcacttc ctgacgtacg ggacggactc aaacctgtac atcgtaggtt attatacgca atgttacaac taaggcttga gcctaattct ggatataaga aatgtgcaag agtggttggt gacgtaatag gtaaatacca cccgcacggt gatgttgcag tatatgatac cttggtacga cttgcacagc atttttcatt gcgttatcct ttgattgacg gtcagggtaa ttttggctct atagatggtg ataatgcagc agctatgcgc tatactgaat cacgtatgac ggaaatatgc atgttattaa tgaaggacat tgataaagat acggtagact tccgttctac ttatgatgat tctgatttag agccggtaat aatgccggca agttttccta atttgcttgc taacggctct gaaggtatag cagttggtat ggcaacaaat ataccaccgc ataatttaca tgagttatgt gatgctttat tgtatttaat cgacaatccg caagctgggg ttaacgatat aatgaacttt atcaaaggcc ctgattttcc aactggtggt ataattattg atcaaactaa agttattaat gctgcatata ctactggacg tggtagtttt agagtaagaa gtagatggga gaaagaggaa ctgagttacg gtacatatca gatagttgtc actgaaatac cttatcaggt acagaaatca aaacttatag aacaaatagc aatattacta aaagataaaa aaattccgct tattagcagt attagagatg aatcaactga tattataagg ttggttatag aaccaagaga tcgtagttgt gatccacaga tagttatgga gtctctattt aagctcacta atttagaaag tcgtattcaa ctaaatatga atgtgatagg tagtaataac gtgccgagag taatgaatat tgtagagatt ctacaagaat ttttagttca tagacaaaat attattactc gtaggtcaac ttatcttttg aataaaatca agcagcgttt agaaatttta aaagtgctta aaatagttta tttaaacctt gacgagataa ttgaaattat ccgtagagag gatgagccaa aaacaataat tatggagcgg tttaaaataa gtgaaataca agtagaagta atattaaaca ctcgtcttcg tgcactccaa aaacttgaag agcatgcaat tattgatgag catagtaatt tgcagaagca acaagctata ttagaaaaga ttttaaaaaa tcataaagaa ctatggaaaa tagtgaaaaa agaaatcaaa tcagtacaaa ctcaatttgg attaaataca ataataggag caaggcgtac aagttttgaa gaagtagatc ttacaaatca agttattgat ataactgcgt tcattactaa agaacctatt actattatct gctcaaaaat ggggtggata cgtttactaa aaggacataa cactgattta tcaactatta aatataaaga aggtgatgcg gaaaagttta ttatagaagc ctatacaact gataaaatat taataattag ttcaaaagga agatttttta ctttgcttgc agataatatt tctaaaggta aaggtactgc tgtatctata aaactacttg tggatatagg taataacgac attactaata ttttagtcta taaaccttat cagcttttat tacttgcaag tagtatcggt aaaggatttt tagttaattc aaacgaagta atagctcaaa caaaagcagg aaagcaaatt atgaatatac cagaggggta tgcatgtata acatgcttac ctgtaaatgg caatagtatt gcttgtatca atgaaagtcg tagattactt atatttaata taaatgaaat acctgaaatg aaaaaagggc aaggtgtagt gctgcaaagg tttaagaatg caaaactttt agatattaaa atatttaata aagaagatgg tttaagttgg aatgatggca aaaaaataca acttgaaaaa aatattgtcg catttttagg gaaaagaggt agtgccggta cattgccacc tattggcttc cctaaaaata atagattttc atcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for RityA.00157.a.B1.PS01631
PURIFICATION DATe: 9/18/2012
CONCENTRATION: 10.1mg/ml
OBSERVED MW: 79kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 91
PERCENT COVERAGE: 45
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK EAKIENIDFG NALSERYLAY ALSTIMSRSL PDVRDGLKPV HRRLLYAMLQ LRLEPNSGYK KCARVVGDVI GKYHPHGDVA VYDTLVRLAQ HFSLRYPLID GQGNFGSIDG DNAAAMRYTE SRMTEICMLL MKDIDKDTVD FRSTYDDSDL EPVIMPASFP NLLANGSEGI AVGMATNIPP HNLHELCDAL LYLIDNPQAG VNDIMNFIKG PDFPTGGIII DQTKVINAAY TTGRGSFRVR SRWEKEELSY GTYQIVVTEI PYQXQKSKLI EQIAILLKDK KIPLIXSXRD ESTDIXXXGY RTXRSXLXSX DSYGVXXLSS LXXKVVXX
Validated NT Sequence
cnnnnnnnnn nnttttgttt aactttaaga aggagaatac catggctcac caccaccacc accatatgaa agaagctaaa atagaaaata ttgactttgg taatgcttta tcagagcgtt atcttgctta tgcattatcg actattatgt cacgttcact tcctgacgta cgggacggac tcaaacctgt acatcgtagg ttattatacg caatgttaca actaaggctt gagcctaatt ctggatataa gaaatgtgca agagtggttg gtgacgtaat aggtaaatac cacccgcacg gtgatgttgc agtatatgat accttggtac gacttgcaca gcatttttca ttgcgttatc ctttgattga cggtcagggt aattttggct ctatagatgg tgataatgca gcagctatgc gctatactga atcacgtatg acggaaatat gcatgttatt aatgaaggac attgataaag atacggtaga cttccgttct acttatgatg attctgattt agagccggta ataatgccgg caagttttcc taatttgctt gctaacggct ctgaaggtat agcagttggt atggcaacaa atataccacc gcataattta catgagttat gtgatgcttt attgtattta atcgacaatc cgcaagctgg ggttaacgat ataatgaact ttatcaaagg ccctgatttt ccnactggtg gtataattat tgatcaaact aaagttatta atgctgcata tactactgga cgtggtagtt ttagagtaag aagtagatgg gagaaagagg aactgagtta cggtacatat cagatagttg tcactgaaat accttatcag gnacagaaat caaaacttat agaacaaata gcaatattac taaaagataa aaaaattccg cttattanca gtantagaga tgaatcaact gatattatna ngntgggtta tagaaccnag agatcgtant tgngatccnc agatagttat ggagtcnnnn ntttaagctc nctantttan aaagtcgtnn tcnnctaann nnngaatgnn gatnngtagt antnannnnn cnnnnagtan ngaannnnnn nnnnnatnct ncannntttn nnnnnaannn nnntnctcnn ngnnannnnn nnntttnnnn naaanccnnn nnnnnnnann ntnnnnnnnn nnnnnnnnnn nnnnnncnan nntnntnnnn nnnnngnnnn nnnnncc
Expressed Protein Sequence
MAHHHHHHMK EAKIENIDFG NALSERYLAY ALSTIMSRSL PDVRDGLKPV HRRLLYAMLQ LRLEPNSGYK KCARVVGDVI GKYHPHGDVA VYDTLVRLAQ HFSLRYPLID GQGNFGSIDG DNAAAMRYTE SRMTEICMLL MKDIDKDTVD FRSTYDDSDL EPVIMPASFP NLLANGSEGI AVGMATNIPP HNLHELCDAL LYLIDNPQAG VNDIMNFIKG PDFPTGGIII DQTKVINAAY TTGRGSFRVR SRWEKEELSY GTYQIVVTEI PYQVQKSKLI EQIAILLKDK KIPLISSIRD ESTDIIRLVI EPRDRSCDPQ IVMESLFKLT NLESRIQLNM NVIGSNNVPR VMNIVEILQE FLVHRQNIIT RRSTYLLNKI KQRLEILKVL KIVYLNLDEI IEIIRREDEP KTIIMERFKI SEIQVEVILN TRLRALQKLE EHAIIDEHSN LQKQQAILEK ILKNHKELWK IVKKEIKSVQ TQFGLNTIIG ARRTSFEEVD LTNQVIDITA FITKEPITII CSKMGWIRLL KGHNTDLSTI KYKEGDAEKF IIEAYTTDKI LIISSKGRFF TLLADNISKG KGTAVSIKLL VDIGNNDITN ILVYKPYQLL LLASSIGKGF LVNSNEVIAQ TKAGKQIMNI PEGYACITCL PVNGNSIACI NESRRLLIFN INEIPEMKKG QGVVLQRFKN AKLLDIKIFN KEDGLSWNDG KKIQLEKNIV AFLGKRGSAG TLPPIGFPKN NRFSS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG AAGCTAAAAT AGAAAATATT GACTTTGGTA ATGCTTTATC AGAGCGTTAT CTTGCTTATG CATTATCGAC TATTATGTCA CGTTCACTTC CTGACGTACG GGACGGACTC AAACCTGTAC ATCGTAGGTT ATTATACGCA ATGTTACAAC TAAGGCTTGA GCCTAATTCT GGATATAAGA AATGTGCAAG AGTGGTTGGT GACGTAATAG GTAAATACCA CCCGCACGGT GATGTTGCAG TATATGATAC CTTGGTACGA CTTGCACAGC ATTTTTCATT GCGTTATCCT TTGATTGACG GTCAGGGTAA TTTTGGCTCT ATAGATGGTG ATAATGCAGC AGCTATGCGC TATACTGAAT CACGTATGAC GGAAATATGC ATGTTATTAA TGAAGGACAT TGATAAAGAT ACGGTAGACT TCCGTTCTAC TTATGATGAT TCTGATTTAG AGCCGGTAAT AATGCCGGCA AGTTTTCCTA ATTTGCTTGC TAACGGCTCT GAAGGTATAG CAGTTGGTAT GGCAACAAAT ATACCACCGC ATAATTTACA TGAGTTATGT GATGCTTTAT TGTATTTAAT CGACAATCCG CAAGCTGGGG TTAACGATAT AATGAACTTT ATCAAAGGCC CTGATTTTCC AACTGGTGGT ATAATTATTG ATCAAACTAA AGTTATTAAT GCTGCATATA CTACTGGACG TGGTAGTTTT AGAGTAAGAA GTAGATGGGA GAAAGAGGAA CTGAGTTACG GTACATATCA GATAGTTGTC ACTGAAATAC CTTATCAGGT ACAGAAATCA AAACTTATAG AACAAATAGC AATATTACTA AAAGATAAAA AAATTCCGCT TATTAGCAGT ATTAGAGATG AATCAACTGA TATTATAAGG TTGGTTATAG AACCAAGAGA TCGTAGTTGT GATCCACAGA TAGTTATGGA GTCTCTATTT AAGCTCACTA ATTTAGAAAG TCGTATTCAA CTAAATATGA ATGTGATAGG TAGTAATAAC GTGCCGAGAG TAATGAATAT TGTAGAGATT CTACAAGAAT TTTTAGTTCA TAGACAAAAT ATTATTACTC GTAGGTCAAC TTATCTTTTG AATAAAATCA AGCAGCGTTT AGAAATTTTA AAAGTGCTTA AAATAGTTTA TTTAAACCTT GACGAGATAA TTGAAATTAT CCGTAGAGAG GATGAGCCAA AAACAATAAT TATGGAGCGG TTTAAAATAA GTGAAATACA AGTAGAAGTA ATATTAAACA CTCGTCTTCG TGCACTCCAA AAACTTGAAG AGCATGCAAT TATTGATGAG CATAGTAATT TGCAGAAGCA ACAAGCTATA TTAGAAAAGA TTTTAAAAAA TCATAAAGAA CTATGGAAAA TAGTGAAAAA AGAAATCAAA TCAGTACAAA CTCAATTTGG ATTAAATACA ATAATAGGAG CAAGGCGTAC AAGTTTTGAA GAAGTAGATC TTACAAATCA AGTTATTGAT ATAACTGCGT TCATTACTAA AGAACCTATT ACTATTATCT GCTCAAAAAT GGGGTGGATA CGTTTACTAA AAGGACATAA CACTGATTTA TCAACTATTA AATATAAAGA AGGTGATGCG GAAAAGTTTA TTATAGAAGC CTATACAACT GATAAAATAT TAATAATTAG TTCAAAAGGA AGATTTTTTA CTTTGCTTGC AGATAATATT TCTAAAGGTA AAGGTACTGC TGTATCTATA AAACTACTTG TGGATATAGG TAATAACGAC ATTACTAATA TTTTAGTCTA TAAACCTTAT CAGCTTTTAT TACTTGCAAG TAGTATCGGT AAAGGATTTT TAGTTAATTC AAACGAAGTA ATAGCTCAAA CAAAAGCAGG AAAGCAAATT ATGAATATAC CAGAGGGGTA TGCATGTATA ACATGCTTAC CTGTAAATGG CAATAGTATT GCTTGTATCA ATGAAAGTCG TAGATTACTT ATATTTAATA TAAATGAAAT ACCTGAAATG AAAAAAGGGC AAGGTGTAGT GCTGCAAAGG TTTAAGAATG CAAAACTTTT AGATATTAAA ATATTTAATA AAGAAGATGG TTTAAGTTGG AATGATGGCA AAAAAATACA ACTTGAAAAA AATATTGTCG CATTTTTAGG GAAAAGAGGT AGTGCCGGTA CATTGCCACC TATTGGCTTC CCTAAAAATA ATAGATTTTC ATCATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT