RityA.00102.a

2-dehydro-3-deoxyphosphooctonate aldolase

CENTER ID:	RityA.00102.a
ORGANISM:	Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE:	Murine typhus (endemic typhus)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.00102.a.B1.GE35404	Full length( RityA.00102.a )		1	274

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130461
PATRIC ID:	fig\|257363.4.peg.71
RefSeq:	YP_067038.1
UniProt:	Q68XT6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKVVKLNNI KIGNDLQFVL IAGPCQIEDE DHALFMAEKL IQLTSKLSIP FIYKSSFDKA NRTSINGIRG LGIEKGLEIL SKVKSEFNCP IITDVHSESQ CTDTAKVVDI LQIPAFLCRQ TDLLKAAAKT GKIVKVKKGQ FLAPWDMKNV QTKLEAFGAK DILFTERGSC FGYNNLISDM RSLAIMSELN VPVVFDATHS VQQPGGRGGS SGGERKYVEL LAKAAISVGI AGIYMEVHQD PDNAPSDGPC MIKLDHLESI LIKLKKYDKI TKEE

NT Sequence

ATGAAAAAAG TAGTAAAACT TAACAATATT AAAATAGGAA ATGATTTACA ATTTGTGCTA ATTGCAGGAC CTTGTCAGAT TGAAGATGAA GACCATGCGT TATTTATGGC AGAAAAACTT ATACAATTAA CCAGTAAGCT TTCGATTCCA TTCATTTATA AATCTTCTTT TGATAAAGCT AATAGAACTT CTATAAATGG AATTAGAGGA CTTGGTATTG AAAAAGGTTT AGAAATTTTA TCAAAAGTTA AATCTGAATT CAATTGCCCT ATTATTACTG ATGTACATTC AGAGAGTCAA TGTACCGACA CAGCTAAAGT AGTAGATATT TTACAAATTC CAGCATTTCT ATGTAGACAA ACCGATTTGC TTAAGGCTGC AGCAAAAACA GGTAAAATAG TAAAAGTAAA AAAAGGACAG TTTCTAGCTC CTTGGGATAT GAAAAATGTA CAAACAAAAC TAGAAGCTTT TGGAGCAAAA GATATCTTAT TTACAGAGCG TGGATCATGT TTTGGTTATA ATAATTTGAT ATCTGATATG CGTAGTCTTG CAATAATGTC GGAGCTAAAT GTACCTGTAG TTTTTGATGC AACACATTCT GTGCAACAGC CTGGAGGGCG AGGAGGTAGT AGCGGTGGAG AACGAAAATA TGTAGAGTTA CTTGCAAAAG CAGCAATTTC TGTAGGAATA GCTGGTATAT ATATGGAAGT ACACCAAGAT CCTGACAATG CTCCAAGTGA TGGTCCATGT ATGATTAAGT TAGATCATTT AGAGTCAATA CTTATCAAAT TAAAAAAATA TGATAAAATT ACAAAAGAAG AA

Details for RityA.00102.a.B1.GE35404

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	31kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK KVVKLNNIKI GNDLQFVLIA GPCQIEDEDH ALFMAEKLIQ LTSKLSIPFI YKSSFDKANR TSINGIRGLG IEKGLEILSK VKSEFNCPII TDVHSESQCT DTAKVVDILQ IPAFLCRQTD LLKAAAKTGK IVKVKKGQFL APWDMKNVQT KLEAFGAKDI LFTERGSCFG YNNLISDMRS LAIMSELNVP VVFDATHSVQ QPGGRGGSSG GERKYVELLA KAAISVGIAG IYMEVHQDPD NAPSDGPCMI KLDHLESILI KLKKYDKITK EE

Validated NT Sequence

tcttactcac ttattcttct tttgtaattt tatcatattt ttttaatttg ataagtattg actctaaatg atctaactta atcatacatg gaccatcact tggagcattg tcaggatctt ggtgtacttc catatatata ccagctattc ctacagaaat tgctgctttt gcaagtaact ctacatattt tcgttctcca ccgctactac ctcctcgccc tccaggctgt tgcacagaat gtgttgcatc aaaaactaca ggtacattta gctccgacat tattgcaaga ctacgcatat cagatatcaa attattataa ccaaaacatg atccacgctc tgtaaataag atatcttttg ctccaaaagc ttctagtttt gtttgtacat ttttcatatc ccaaggagct agaaactgtc ctttttttac ttttactatt ttacctgttt ttgctgcagc cttaagcaaa tcggtttgtc tacatagaaa tgctggaatt tgtaaaatat ctactacttt agctgtgtcg gtacattgac tctctgaatg tacatcagta ataatagggc aattgaattc agatttaact tttgataaaa tttctaaacc tttttcaata ccaagtcctc taattccatt tatagaagtt ctattagctt tatcaaaaga agatttataa atgaatggaa tcgaaagctt actggttaat tgtataagtt tttctgccat aaataacgca tggtcttcat cttcaatctg acaaggtcct gcaattagca caaattgtaa atcatttcct attttaatat tgttaagttt tactactttt ttcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aagtagtaaa acttaacaat attaaaatag gaaatgattt acaatttgtg ctaattgcag gaccttgtca gattgaagat gaagaccatg cgttatttat ggcagaaaaa cttatacaat taaccagtaa gctttcgatt ccattcattt ataaatcttc ttttgataaa gctaatagaa cttctataaa tggaattaga ggacttggta ttgaaaaagg tttagaaatt ttatcaaaag ttaaatctga attcaattgc cctattatta ctgatgtaca ttcagagagt caatgtaccg acacagctaa agtagtagat attttacaaa ttccagcatt tctatgtaga caaaccgatt tgcttaaggc tgcagcaaaa acaggtaaaa tagtaaaagt aaaaaaagga cagtttctag ctccttggga tatgaaaaat gtacaaacaa aactagaagc ttttggagca aaagatatct tatttacaga gcgtggatca tgttttggtt ataataattt gatatctgat atgcgtagtc ttgcaataat gtcggagcta aatgtacctg tagtttttga tgcaacacat tctgtgcaac agcctggagg gcgaggaggt agtagcggtg gagaacgaaa atatgtagag ttacttgcaa aagcagcaat ttctgtagga atagctggta tatatatgga agtacaccaa gatcctgaca atgctccaag tgatggtcca tgtatgatta agttagatca tttagagtca atacttatca aattaaaaaa atatgataaa attacaaaag aagaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat