RityA.00081.a

Cysteine desulfurase protein IscS/NifS

CENTER ID: RityA.00081.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.00081.a.B1.GE35395 Full length( RityA.00081.a ) 1 378
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.00081.a.B1.PS01599 Full length( RityA.00081.a ) 1 378
RityA.00081.a.B1.PS01600 Full length( RityA.00081.a ) 1 378

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|257363.4.peg.503
RefSeq: YP_067429.1
UniProt: Q68WP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIYLDHNATT FIDPRVKEYI ISLMDKELNP SSTHMAGRFA QNIIETARSQ IATSLGITML SGEYNITFTS SGTESNNLIM KNFYDGDIFI SAIEHLSIYN HINYAPNIKV IRVNKQGLVD LEHLEDLLFQ SNARKKLVSV MIANNENGVL QDIAAISKIT KKYDAKFHSD LVQSFGKISL NIKELGLDFA TISGHKIGGG HGCGALISNS NFQLIPIIIG GGQEKSVRSG TENVLAIAGF GLAAEFRRKN VSENYIKIRD LQERLEQKLK KYSNVNIISN NVARLANTTL ITVDNTDAQV KLIEFDLRNI CVSSGSACSS GKISKSHVLT NMGIREEEAK SSIRISLSHT NTVSDIKAFI KAFEEIYEYK PSSNHGIK
NT Sequence
ATGATATATC TTGATCATAA TGCGACTACT TTCATCGATC CGAGAGTTAA GGAATATATA ATAAGTTTAA TGGATAAGGA ACTTAACCCT TCATCTACGC ATATGGCTGG CAGATTTGCT CAGAACATTA TAGAAACGGC ACGTTCACAA ATAGCAACAT CTCTTGGTAT AACAATGTTA TCTGGAGAAT ATAATATTAC TTTTACATCT TCTGGAACTG AAAGTAATAA TTTAATAATG AAAAATTTTT ATGATGGTGA TATTTTTATT TCAGCTATTG AACATTTATC GATTTATAAT CATATAAATT ATGCTCCAAA TATTAAAGTT ATAAGAGTTA ATAAGCAAGG ACTAGTTGAT TTAGAACATC TAGAAGACTT ATTATTTCAA AGTAATGCTA GAAAAAAATT AGTTTCTGTA ATGATCGCAA ATAATGAAAA TGGAGTTTTA CAAGATATAG CTGCGATAAG TAAAATAACT AAAAAATATG ATGCAAAATT TCATAGTGAT TTAGTACAGA GTTTTGGTAA AATATCTTTA AATATTAAAG AATTAGGGTT AGATTTTGCT ACAATTTCAG GACATAAAAT AGGAGGAGGG CATGGTTGTG GTGCTTTAAT TTCTAACTCT AATTTTCAGC TTATACCAAT AATTATAGGA GGAGGCCAAG AAAAATCAGT ACGCTCAGGT ACTGAAAATG TTTTAGCAAT TGCCGGCTTT GGTTTAGCTG CTGAATTTAG AAGAAAAAAT GTTTCAGAGA ATTACATAAA AATCAGGGAT TTGCAAGAAA GATTAGAGCA AAAACTAAAA AAATATTCAA ACGTAAATAT TATTAGTAAT AACGTAGCTA GGTTAGCTAA TACTACCTTA ATTACTGTCG ACAATACTGA TGCACAAGTA AAATTAATAG AGTTCGATTT ACGTAATATT TGTGTAAGTT CTGGGTCTGC TTGCTCATCA GGCAAAATAT CTAAATCGCA TGTATTAACC AATATGGGCA TAAGAGAAGA AGAAGCTAAG TCTTCAATTC GTATATCTTT AAGTCATACT AATACAGTAA GTGATATAAA GGCTTTTATA AAAGCTTTTG AGGAGATATA TGAATATAAA CCGTCTAGTA ACCACGGTAT CAAG
Details for RityA.00081.a.B1.GE35395
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 42kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Validated AA Sequence
MDKELNPSST HMAGRFAQNI IETARSQIAT SLGITMLSGE YNITFTSSGT ESNNLIMKNF YDGDIFISAI EHLSIYNHIN YAPNIKVIRV NKQGLVDLEH LEDLLFQSNA RKKLVSVMIA NNENGVLQDI AAISKITKKY DAKFHSDLVQ SFGKISLNIK ELGLDFATIS GHKIGGGHGC GALISNSNFQ LIPIIIGGGQ EKSVRSGTEN VLAIAGFGLA AEFRRKNVSE NYIKIRDLQE RLEQKLKKYS NVNIISNNVA RLANTTLITV DNTDAQVKLI EFDLRNICVS SGSACSSGKI SKSHVLTNMG IREEEAKSSI RISLSHTNTV SDIKAFIKAF EEIYEY
Validated NT Sequence
tatattcata tatctcctca aaagctttta taaaagcctt tatatcactt actgtattag tatgacttaa agatatacga attgaagact tagcttcttc ttctcttatg cccatattgg ttaatacatg cgatttagat attttgcctg atgagcaagc agacccagaa cttacacaaa tattacgtaa atcgaactct attaatttta cttgtgcatc agtattgtcg acagtaatta aggtagtatt agctaaccta gctacgttat tactaataat atttacgttt gaatattttt ttagtttttg ctctaatctt tcttgcaaat ccctgatttt tatgtaattc tctgaaacat tttttcttct aaattcagca gctaaaccaa agccggcaat tgctaaaaca ttttcagtac ctgagcgtac tgatttttct tggcctcctc ctataattat tggtataagc tgaaaattag agttagaaat taaagcacca caaccatgcc ctcctcctat tttatgtcct gaaattgtag caaaatctaa ccctaattct ttaatattta aagatatttt accaaaactc tgtactaaat cactatgaaa ttttgcatca tattttttag ttattttact tatcgcagct atatcttgta aaactccatt ttcattattt gcgatcatta cagaaactaa tttttttcta gcattacttt gaaataataa gtcttctaga tgttctaaat caactagtcc ttgcttatta actcttataa ctttaatatt tggagcataa tttatatgat tataaatcga taaatgttca atagctgaaa taaaaatatc accatcataa aaatttttca ttattaaatt attactttca gttccagaag atgtaaaagt aatattatat tctccagata acattgttat accaagagat gttgctattt gtgaacgtgc cgtttctata atgttctgag caaatctgcc agccatatgc gtagatgaag ggttaagttc cttatccatt aaacttatta tatattcctt aactctcgga tcgatgaaag tagtcgcatt atgatcaaga ta
Expected Protein Sequence
MAHHHHHHMI YLDHNATTFI DPRVKEYIIS LMDKELNPSS THMAGRFAQN IIETARSQIA TSLGITMLSG EYNITFTSSG TESNNLIMKN FYDGDIFISA IEHLSIYNHI NYAPNIKVIR VNKQGLVDLE HLEDLLFQSN ARKKLVSVMI ANNENGVLQD IAAISKITKK YDAKFHSDLV QSFGKISLNI KELGLDFATI SGHKIGGGHG CGALISNSNF QLIPIIIGGG QEKSVRSGTE NVLAIAGFGL AAEFRRKNVS ENYIKIRDLQ ERLEQKLKKY SNVNIISNNV ARLANTTLIT VDNTDAQVKL IEFDLRNICV SSGSACSSGK ISKSHVLTNM GIREEEAKSS IRISLSHTNT VSDIKAFIKA FEEIYEYKPS SNHGIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatat atcttgatca taatgcgact actttcatcg atccgagagt taaggaatat ataataagtt taatggataa ggaacttaac ccttcatcta cgcatatggc tggcagattt gctcagaaca ttatagaaac ggcacgttca caaatagcaa catctcttgg tataacaatg ttatctggag aatataatat tacttttaca tcttctggaa ctgaaagtaa taatttaata atgaaaaatt tttatgatgg tgatattttt atttcagcta ttgaacattt atcgatttat aatcatataa attatgctcc aaatattaaa gttataagag ttaataagca aggactagtt gatttagaac atctagaaga cttattattt caaagtaatg ctagaaaaaa attagtttct gtaatgatcg caaataatga aaatggagtt ttacaagata tagctgcgat aagtaaaata actaaaaaat atgatgcaaa atttcatagt gatttagtac agagttttgg taaaatatct ttaaatatta aagaattagg gttagatttt gctacaattt caggacataa aataggagga gggcatggtt gtggtgcttt aatttctaac tctaattttc agcttatacc aataattata ggaggaggcc aagaaaaatc agtacgctca ggtactgaaa atgttttagc aattgccggc tttggtttag ctgctgaatt tagaagaaaa aatgtttcag agaattacat aaaaatcagg gatttgcaag aaagattaga gcaaaaacta aaaaaatatt caaacgtaaa tattattagt aataacgtag ctaggttagc taatactacc ttaattactg tcgacaatac tgatgcacaa gtaaaattaa tagagttcga tttacgtaat atttgtgtaa gttctgggtc tgcttgctca tcaggcaaaa tatctaaatc gcatgtatta accaatatgg gcataagaga agaagaagct aagtcttcaa ttcgtatatc tttaagtcat actaatacag taagtgatat aaaggctttt ataaaagctt ttgaggagat atatgaatat aaaccgtcta gtaaccacgg tatcaagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for RityA.00081.a.B1.PS01599
PURIFICATION DATe: 8/15/2012
CONCENTRATION: 64.22mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MDKELNPSST HMAGRFAQNI IETARSQIAT SLGITMLSGE YNITFTSSGT ESNNLIMKNF YDGDIFISAI EHLSIYNHIN YAPNIKVIRV NKQGLVDLEH LEDLLFQSNA RKKLVSVMIA NNENGVLQDI AAISKITKKY DAKFHSDLVQ SFGKISLNIK ELGLDFATIS GHKIGGGHGC GALISNSNFQ LIPIIIGGGQ EKSVRSGTEN VLAIAGFGLA AEFRRKNVSE NYIKIRDLQE RLEQKLKKYS NVNIISNNVA RLANTTLITV DNTDAQVKLI EFDLRNICVS SGSACSSGKI SKSHVLTNMG IREEEAKSSI RISLSHTNTV SDIKAFIKAF EEIYEY
Validated NT Sequence
tatattcata tatctcctca aaagctttta taaaagcctt tatatcactt actgtattag tatgacttaa agatatacga attgaagact tagcttcttc ttctcttatg cccatattgg ttaatacatg cgatttagat attttgcctg atgagcaagc agacccagaa cttacacaaa tattacgtaa atcgaactct attaatttta cttgtgcatc agtattgtcg acagtaatta aggtagtatt agctaaccta gctacgttat tactaataat atttacgttt gaatattttt ttagtttttg ctctaatctt tcttgcaaat ccctgatttt tatgtaattc tctgaaacat tttttcttct aaattcagca gctaaaccaa agccggcaat tgctaaaaca ttttcagtac ctgagcgtac tgatttttct tggcctcctc ctataattat tggtataagc tgaaaattag agttagaaat taaagcacca caaccatgcc ctcctcctat tttatgtcct gaaattgtag caaaatctaa ccctaattct ttaatattta aagatatttt accaaaactc tgtactaaat cactatgaaa ttttgcatca tattttttag ttattttact tatcgcagct atatcttgta aaactccatt ttcattattt gcgatcatta cagaaactaa tttttttcta gcattacttt gaaataataa gtcttctaga tgttctaaat caactagtcc ttgcttatta actcttataa ctttaatatt tggagcataa tttatatgat tataaatcga taaatgttca atagctgaaa taaaaatatc accatcataa aaatttttca ttattaaatt attactttca gttccagaag atgtaaaagt aatattatat tctccagata acattgttat accaagagat gttgctattt gtgaacgtgc cgtttctata atgttctgag caaatctgcc agccatatgc gtagatgaag ggttaagttc cttatccatt aaacttatta tatattcctt aactctcgga tcgatgaaag tagtcgcatt atgatcaaga ta
Expressed Protein Sequence
MAHHHHHHMI YLDHNATTFI DPRVKEYIIS LMDKELNPSS THMAGRFAQN IIETARSQIA TSLGITMLSG EYNITFTSSG TESNNLIMKN FYDGDIFISA IEHLSIYNHI NYAPNIKVIR VNKQGLVDLE HLEDLLFQSN ARKKLVSVMI ANNENGVLQD IAAISKITKK YDAKFHSDLV QSFGKISLNI KELGLDFATI SGHKIGGGHG CGALISNSNF QLIPIIIGGG QEKSVRSGTE NVLAIAGFGL AAEFRRKNVS ENYIKIRDLQ ERLEQKLKKY SNVNIISNNV ARLANTTLIT VDNTDAQVKL IEFDLRNICV SSGSACSSGK ISKSHVLTNM GIREEEAKSS IRISLSHTNT VSDIKAFIKA FEEIYEYKPS SNHGIK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATAT ATCTTGATCA TAATGCGACT ACTTTCATCG ATCCGAGAGT TAAGGAATAT ATAATAAGTT TAATGGATAA GGAACTTAAC CCTTCATCTA CGCATATGGC TGGCAGATTT GCTCAGAACA TTATAGAAAC GGCACGTTCA CAAATAGCAA CATCTCTTGG TATAACAATG TTATCTGGAG AATATAATAT TACTTTTACA TCTTCTGGAA CTGAAAGTAA TAATTTAATA ATGAAAAATT TTTATGATGG TGATATTTTT ATTTCAGCTA TTGAACATTT ATCGATTTAT AATCATATAA ATTATGCTCC AAATATTAAA GTTATAAGAG TTAATAAGCA AGGACTAGTT GATTTAGAAC ATCTAGAAGA CTTATTATTT CAAAGTAATG CTAGAAAAAA ATTAGTTTCT GTAATGATCG CAAATAATGA AAATGGAGTT TTACAAGATA TAGCTGCGAT AAGTAAAATA ACTAAAAAAT ATGATGCAAA ATTTCATAGT GATTTAGTAC AGAGTTTTGG TAAAATATCT TTAAATATTA AAGAATTAGG GTTAGATTTT GCTACAATTT CAGGACATAA AATAGGAGGA GGGCATGGTT GTGGTGCTTT AATTTCTAAC TCTAATTTTC AGCTTATACC AATAATTATA GGAGGAGGCC AAGAAAAATC AGTACGCTCA GGTACTGAAA ATGTTTTAGC AATTGCCGGC TTTGGTTTAG CTGCTGAATT TAGAAGAAAA AATGTTTCAG AGAATTACAT AAAAATCAGG GATTTGCAAG AAAGATTAGA GCAAAAACTA AAAAAATATT CAAACGTAAA TATTATTAGT AATAACGTAG CTAGGTTAGC TAATACTACC TTAATTACTG TCGACAATAC TGATGCACAA GTAAAATTAA TAGAGTTCGA TTTACGTAAT ATTTGTGTAA GTTCTGGGTC TGCTTGCTCA TCAGGCAAAA TATCTAAATC GCATGTATTA ACCAATATGG GCATAAGAGA AGAAGAAGCT AAGTCTTCAA TTCGTATATC TTTAAGTCAT ACTAATACAG TAAGTGATAT AAAGGCTTTT ATAAAAGCTT TTGAGGAGAT ATATGAATAT AAACCGTCTA GTAACCACGG TATCAAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for RityA.00081.a.B1.PS01600
PURIFICATION DATe: 8/15/2012
CONCENTRATION: 47.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MDKELNPSST HMAGRFAQNI IETARSQIAT SLGITMLSGE YNITFTSSGT ESNNLIMKNF YDGDIFISAI EHLSIYNHIN YAPNIKVIRV NKQGLVDLEH LEDLLFQSNA RKKLVSVMIA NNENGVLQDI AAISKITKKY DAKFHSDLVQ SFGKISLNIK ELGLDFATIS GHKIGGGHGC GALISNSNFQ LIPIIIGGGQ EKSVRSGTEN VLAIAGFGLA AEFRRKNVSE NYIKIRDLQE RLEQKLKKYS NVNIISNNVA RLANTTLITV DNTDAQVKLI EFDLRNICVS SGSACSSGKI SKSHVLTNMG IREEEAKSSI RISLSHTNTV SDIKAFIKAF EEIYEY
Validated NT Sequence
tatattcata tatctcctca aaagctttta taaaagcctt tatatcactt actgtattag tatgacttaa agatatacga attgaagact tagcttcttc ttctcttatg cccatattgg ttaatacatg cgatttagat attttgcctg atgagcaagc agacccagaa cttacacaaa tattacgtaa atcgaactct attaatttta cttgtgcatc agtattgtcg acagtaatta aggtagtatt agctaaccta gctacgttat tactaataat atttacgttt gaatattttt ttagtttttg ctctaatctt tcttgcaaat ccctgatttt tatgtaattc tctgaaacat tttttcttct aaattcagca gctaaaccaa agccggcaat tgctaaaaca ttttcagtac ctgagcgtac tgatttttct tggcctcctc ctataattat tggtataagc tgaaaattag agttagaaat taaagcacca caaccatgcc ctcctcctat tttatgtcct gaaattgtag caaaatctaa ccctaattct ttaatattta aagatatttt accaaaactc tgtactaaat cactatgaaa ttttgcatca tattttttag ttattttact tatcgcagct atatcttgta aaactccatt ttcattattt gcgatcatta cagaaactaa tttttttcta gcattacttt gaaataataa gtcttctaga tgttctaaat caactagtcc ttgcttatta actcttataa ctttaatatt tggagcataa tttatatgat tataaatcga taaatgttca atagctgaaa taaaaatatc accatcataa aaatttttca ttattaaatt attactttca gttccagaag atgtaaaagt aatattatat tctccagata acattgttat accaagagat gttgctattt gtgaacgtgc cgtttctata atgttctgag caaatctgcc agccatatgc gtagatgaag ggttaagttc cttatccatt aaacttatta tatattcctt aactctcgga tcgatgaaag tagtcgcatt atgatcaaga ta
Expressed Protein Sequence
MAHHHHHHMI YLDHNATTFI DPRVKEYIIS LMDKELNPSS THMAGRFAQN IIETARSQIA TSLGITMLSG EYNITFTSSG TESNNLIMKN FYDGDIFISA IEHLSIYNHI NYAPNIKVIR VNKQGLVDLE HLEDLLFQSN ARKKLVSVMI ANNENGVLQD IAAISKITKK YDAKFHSDLV QSFGKISLNI KELGLDFATI SGHKIGGGHG CGALISNSNF QLIPIIIGGG QEKSVRSGTE NVLAIAGFGL AAEFRRKNVS ENYIKIRDLQ ERLEQKLKKY SNVNIISNNV ARLANTTLIT VDNTDAQVKL IEFDLRNICV SSGSACSSGK ISKSHVLTNM GIREEEAKSS IRISLSHTNT VSDIKAFIKA FEEIYEYKPS SNHGIK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATAT ATCTTGATCA TAATGCGACT ACTTTCATCG ATCCGAGAGT TAAGGAATAT ATAATAAGTT TAATGGATAA GGAACTTAAC CCTTCATCTA CGCATATGGC TGGCAGATTT GCTCAGAACA TTATAGAAAC GGCACGTTCA CAAATAGCAA CATCTCTTGG TATAACAATG TTATCTGGAG AATATAATAT TACTTTTACA TCTTCTGGAA CTGAAAGTAA TAATTTAATA ATGAAAAATT TTTATGATGG TGATATTTTT ATTTCAGCTA TTGAACATTT ATCGATTTAT AATCATATAA ATTATGCTCC AAATATTAAA GTTATAAGAG TTAATAAGCA AGGACTAGTT GATTTAGAAC ATCTAGAAGA CTTATTATTT CAAAGTAATG CTAGAAAAAA ATTAGTTTCT GTAATGATCG CAAATAATGA AAATGGAGTT TTACAAGATA TAGCTGCGAT AAGTAAAATA ACTAAAAAAT ATGATGCAAA ATTTCATAGT GATTTAGTAC AGAGTTTTGG TAAAATATCT TTAAATATTA AAGAATTAGG GTTAGATTTT GCTACAATTT CAGGACATAA AATAGGAGGA GGGCATGGTT GTGGTGCTTT AATTTCTAAC TCTAATTTTC AGCTTATACC AATAATTATA GGAGGAGGCC AAGAAAAATC AGTACGCTCA GGTACTGAAA ATGTTTTAGC AATTGCCGGC TTTGGTTTAG CTGCTGAATT TAGAAGAAAA AATGTTTCAG AGAATTACAT AAAAATCAGG GATTTGCAAG AAAGATTAGA GCAAAAACTA AAAAAATATT CAAACGTAAA TATTATTAGT AATAACGTAG CTAGGTTAGC TAATACTACC TTAATTACTG TCGACAATAC TGATGCACAA GTAAAATTAA TAGAGTTCGA TTTACGTAAT ATTTGTGTAA GTTCTGGGTC TGCTTGCTCA TCAGGCAAAA TATCTAAATC GCATGTATTA ACCAATATGG GCATAAGAGA AGAAGAAGCT AAGTCTTCAA TTCGTATATC TTTAAGTCAT ACTAATACAG TAAGTGATAT AAAGGCTTTT ATAAAAGCTT TTGAGGAGAT ATATGAATAT AAACCGTCTA GTAACCACGG TATCAAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT