SSGCID: Target Page

RiriA.18696.a

Putative uncharacterized protein

CENTER ID:	RiriA.18696.a
ORGANISM:	Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE:	Rocky Mountain Spotted fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiriA.18696.a.B1.GE37592	Full length(RiriA.18696.a)		1	332
RiriA.18696.a.B2.GE37593	domain based on Ec 2F1M alignment		31	317

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiriA.18696.a.B2.PS02023	domain based on Ec 2F1M alignment		31	317

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|392021.5.peg.551
RefSeq:	YP_001494577.1
UniProt:	A0A0H3AWM4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMSAKLIVLF TLLISLSTMA SKKEEETITA VKATKVQIAE LYDIFNIVGQ CQNDNSRDYY ANATGVVEQV SAHQGEIVKK GDILLVIDKN IAETTKSRAA ALLNTKQEDY NRKAALFAKK FVSNEEYKRS KSELEDAKFN YSKALKTYND MIITAPYSGK IGVIKSMVGD EVKRGDYLFS ITGTENSQSI FIELPESLSG KVGVDTEVLI AGKIKSTIGA ISHYLSDNGT LTAKIIVPMG TKILHNSFVD VMLIINPHKN LTVPASCIQR NNQGNFIYKI DGYTIKQLYV KIGTRTEGLI EIISDDIKAG DLVVTEGMTK IGDGSKVKIL EE

NT Sequence

ATGATGTCTG CAAAACTTAT TGTTCTTTTT ACCTTACTTA TCTCGCTTTC TACTATGGCA AGTAAAAAGG AAGAAGAAAC AATTACAGCA GTAAAAGCAA CTAAAGTACA AATTGCTGAG CTTTACGACA TATTTAATAT TGTAGGACAA TGCCAGAATG ATAATAGTCG TGATTATTAT GCCAATGCCA CCGGAGTAGT TGAACAAGTC TCAGCACATC AAGGAGAAAT AGTAAAAAAA GGGGATATAT TGCTAGTTAT AGATAAAAAC ATAGCAGAAA CCACTAAATC TAGAGCAGCA GCGTTATTAA ATACAAAACA AGAAGATTAT AATAGAAAAG CAGCTTTATT CGCTAAAAAA TTTGTAAGCA ACGAGGAATA TAAAAGGTCA AAAAGCGAAC TTGAAGATGC AAAATTTAAT TATTCCAAAG CTCTAAAAAC ATATAATGAT ATGATAATTA CTGCACCCTA CTCTGGTAAA ATAGGCGTAA TTAAGTCTAT GGTCGGCGAT GAAGTAAAAA GAGGTGACTA TCTTTTTAGC ATTACCGGAA CAGAAAACTC ACAAAGTATT TTTATTGAAT TACCGGAGTC TTTAAGCGGA AAAGTTGGCG TCGATACTGA AGTTTTAATT GCAGGAAAAA TTAAAAGTAC AATCGGGGCA ATATCGCATT ACTTATCAGA TAATGGTACT TTAACAGCTA AAATTATTGT ACCTATGGGT ACAAAAATCC TGCATAATAG CTTTGTAGAT GTTATGCTTA TAATTAACCC TCATAAAAAC CTAACCGTTC CTGCAAGTTG TATTCAACGC AATAATCAAG GTAATTTTAT TTATAAAATA GACGGTTATA CGATAAAACA ATTATATGTA AAAATCGGTA CACGTACAGA AGGTCTAATT GAAATTATCT CTGACGATAT TAAAGCAGGT GACTTAGTCG TTACCGAAGG TATGACTAAA ATTGGTGATG GCTCAAAAGT TAAGATACTT GAGGAA

Details for RiriA.18696.a.B1.GE37592

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	38kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	96
PERCENT COVERAGE:	84

Validated AA Sequence

MAHHHHHHMV KATKVQIAEL YDIFNIVGQC QNDNSRDYYA NATGVVEQVS AHQGEIVKKG DILLVIDKNI AETTKSRAAA LLNTKQEDYN SKEALFAKKF VSNEEYKRSK SELEDAKFNY AKALKTYNDM IITAPYSGKI GVIKSMVGDE VKIGDYLFSI TGTENSQGIF IELPESLSGK VGVDTEVLIA GKIKSTIGAV SHYLSDNGTL TAKIILPMGT KILHNSFVDV TLIINPHKNL TVPESCIQRN NQGTFIYKIE GYTIKQLYVK IGTRTEDLIE IISDDIKAGD LVVTEG

Validated NT Sequence

ttaaccttcg gtaacgacta agtcacctgc tttaatatcg tcagagataa tttcaattag atcttctgta cgtgtaccga tttttacata taattgtttt atcgtataac cttctatttt ataaataaaa gtaccttgat tatttcgttg aatacaactt tcaggaacgg ttaggttttt atgagggtta attataagcg taacatctac aaagctatta tgcaggattt ttgtacccat aggtaaaata attttagctg ttaaagtacc attatctgat aagtaatgcg atactgcccc gattgtactt ttaatttttc ctgcaattaa aacttcagta tcgacgccaa cttttccgct taaagactcc ggcaattcaa taaaaatacc ttgtgagttt tctgttccgg taatgctaaa aagatagtca cctattttta cttcatcgcc gaccatagac ttaattacgc ctattttacc agagtagggt gcagtaatta tcatatcatt atatgttttt agagctttgg cataattaaa ttttgcatct tcaagttcgc tttttgacct tttatattcc tcgttgctta caaatttttt agcgaataaa gcttctttgc tattataatc ttcttgtttt gtatttaata acgctgctgc tctagattta gtggtttctg ctatgttttt atctataact agcaatatat cccctttttt tactatttct ccttgatgtg ctgagacttg ttcaactact ccggtggcat tggcataata atcacgacta ttatcattct ggcattgccc tacaatatta aatatgtcgt aaagctcagc aatttgtact ttagttgctt ttaccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHMM SAKLIVLFTL LISLSTMASK KEEETITAVK ATKVQIAELY DIFNIVGQCQ NDNSRDYYAN ATGVVEQVSA HQGEIVKKGD ILLVIDKNIA ETTKSRAAAL LNTKQEDYNR KAALFAKKFV SNEEYKRSKS ELEDAKFNYS KALKTYNDMI ITAPYSGKIG VIKSMVGDEV KRGDYLFSIT GTENSQSIFI ELPESLSGKV GVDTEVLIAG KIKSTIGAIS HYLSDNGTLT AKIIVPMGTK ILHNSFVDVM LIINPHKNLT VPASCIQRNN QGNFIYKIDG YTIKQLYVKI GTRTEGLIEI ISDDIKAGDL VVTEGMTKIG DGSKVKILEE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgt ctgcaaaact tattgttctt tttaccttac ttatctcgct ttctactatg gcaagtaaaa aggaagaaga aacaattaca gcagtaaaag caactaaagt acaaattgct gagctttacg acatatttaa tattgtagga caatgccaga atgataatag tcgtgattat tatgccaatg ccaccggagt agttgaacaa gtctcagcac atcaaggaga aatagtaaaa aaaggggata tattgctagt tatagataaa aacatagcag aaaccactaa atctagagca gcagcgttat taaatacaaa acaagaagat tataatagaa aagcagcttt attcgctaaa aaatttgtaa gcaacgagga atataaaagg tcaaaaagcg aacttgaaga tgcaaaattt aattattcca aagctctaaa aacatataat gatatgataa ttactgcacc ctactctggt aaaataggcg taattaagtc tatggtcggc gatgaagtaa aaagaggtga ctatcttttt agcattaccg gaacagaaaa ctcacaaagt atttttattg aattaccgga gtctttaagc ggaaaagttg gcgtcgatac tgaagtttta attgcaggaa aaattaaaag tacaatcggg gcaatatcgc attacttatc agataatggt actttaacag ctaaaattat tgtacctatg ggtacaaaaa tcctgcataa tagctttgta gatgttatgc ttataattaa ccctcataaa aacctaaccg ttcctgcaag ttgtattcaa cgcaataatc aaggtaattt tatttataaa atagacggtt atacgataaa acaattatat gtaaaaatcg gtacacgtac agaaggtcta attgaaatta tctctgacga tattaaagca ggtgacttag tcgttaccga aggtatgact aaaattggtg atggctcaaa agttaagata cttgaggaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for RiriA.18696.a.B2.GE37593

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	56

Validated AA Sequence

MIITAPYSGK IGVIKSMVGD EVKRGDYLFS ITGTENSQSI FIELPESLSG KVGVDTEVLI AGKIKSTIGA ISHYLSDNGT LTAKIIVPMG TKILHNSFVD VMLIINPHKN LTVPASCIQR NNQGNFIYKI DGYTIKQLYV KIGTRTEGLI EIISDDIKAG DLVVTEG

Validated NT Sequence

atcttactca cttaaccttc ggtaacgact aagtcacctg ctttaatatc gtcagagata atttcaatta gaccttctgt acgtgtaccg atttttacat ataattgttt tatcgtataa ccgtctattt tataaataaa attaccttga ttattgcgtt gaatacaact tgcaggaacg gttaggtttt tatgagggtt aattataagc ataacatcta caaagctatt atgcaggatt tttgtaccca taggtacaat aattttagct gttaaagtac cattatctga taagtaatgc gatattgccc cgattgtact tttaattttt cctgcaatta aaacttcagt atcgacgcca acttttccgc ttaaagactc cggtaattca ataaaaatac tttgtgagtt ttctgttccg gtaatgctaa aaagatagtc acctcttttt acttcatcgc cgaccataga cttaattacg cctattttac cagagtaggg tgcagtaatt atcatatcat tatatgtttt tagagctttg gaataattaa attttgcatc ttcaagttcg ctttttgacc ttttatattc ctcgttgctt acaaattttt tagcgaataa agctgctttt ctattataat cttcttgttt tgtatttaat aacgctgctg ctctagattt agtggtttct gctatgtttt tatctataac tagcaatata tccccttttt ttactatttc tccttgatgt gctgagactt gttcaactac tccggtggca ttggcataat aatcacgact attatcattc tggcattgtc ctacaatatt aaatatgtcg taaagctcag caatttgtac

Expected Protein Sequence

MAHHHHHHVK ATKVQIAELY DIFNIVGQCQ NDNSRDYYAN ATGVVEQVSA HQGEIVKKGD ILLVIDKNIA ETTKSRAAAL LNTKQEDYNR KAALFAKKFV SNEEYKRSKS ELEDAKFNYS KALKTYNDMI ITAPYSGKIG VIKSMVGDEV KRGDYLFSIT GTENSQSIFI ELPESLSGKV GVDTEVLIAG KIKSTIGAIS HYLSDNGTLT AKIIVPMGTK ILHNSFVDVM LIINPHKNLT VPASCIQRNN QGNFIYKIDG YTIKQLYVKI GTRTEGLIEI ISDDIKAGDL VVTEG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtaaaag caactaaagt acaaattgct gagctttacg acatatttaa tattgtagga caatgccaga atgataatag tcgtgattat tatgccaatg ccaccggagt agttgaacaa gtctcagcac atcaaggaga aatagtaaaa aaaggggata tattgctagt tatagataaa aacatagcag aaaccactaa atctagagca gcagcgttat taaatacaaa acaagaagat tataatagaa aagcagcttt attcgctaaa aaatttgtaa gcaacgagga atataaaagg tcaaaaagcg aacttgaaga tgcaaaattt aattattcca aagctctaaa aacatataat gatatgataa ttactgcacc ctactctggt aaaataggcg taattaagtc tatggtcggc gatgaagtaa aaagaggtga ctatcttttt agcattaccg gaacagaaaa ctcacaaagt atttttattg aattaccgga gtctttaagc ggaaaagttg gcgtcgatac tgaagtttta attgcaggaa aaattaaaag tacaatcggg gcaatatcgc attacttatc agataatggt actttaacag ctaaaattat tgtacctatg ggtacaaaaa tcctgcataa tagctttgta gatgttatgc ttataattaa ccctcataaa aacctaaccg ttcctgcaag ttgtattcaa cgcaataatc aaggtaattt tatttataaa atagacggtt atacgataaa acaattatat gtaaaaatcg gtacacgtac agaaggtcta attgaaatta tctctgacga tattaaagca ggtgacttag tcgttaccga aggttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for RiriA.18696.a.B2.PS02023

PURIFICATION DATe:	3/20/2014
CONCENTRATION:	1mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	56

Protocol Notes

notes unavailable

Validated AA Sequence

MIITAPYSGK IGVIKSMVGD EVKRGDYLFS ITGTENSQSI FIELPESLSG KVGVDTEVLI AGKIKSTIGA ISHYLSDNGT LTAKIIVPMG TKILHNSFVD VMLIINPHKN LTVPASCIQR NNQGNFIYKI DGYTIKQLYV KIGTRTEGLI EIISDDIKAG DLVVTEG

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTAAAAG CAACTAAAGT ACAAATTGCT GAGCTTTACG ACATATTTAA TATTGTAGGA CAATGCCAGA ATGATAATAG TCGTGATTAT TATGCCAATG CCACCGGAGT AGTTGAACAA GTCTCAGCAC ATCAAGGAGA AATAGTAAAA AAAGGGGATA TATTGCTAGT TATAGATAAA AACATAGCAG AAACCACTAA ATCTAGAGCA GCAGCGTTAT TAAATACAAA ACAAGAAGAT TATAATAGAA AAGCAGCTTT ATTCGCTAAA AAATTTGTAA GCAACGAGGA ATATAAAAGG TCAAAAAGCG AACTTGAAGA TGCAAAATTT AATTATTCCA AAGCTCTAAA AACATATAAT GATATGATAA TTACTGCACC CTACTCTGGT AAAATAGGCG TAATTAAGTC TATGGTCGGC GATGAAGTAA AAAGAGGTGA CTATCTTTTT AGCATTACCG GAACAGAAAA CTCACAAAGT ATTTTTATTG AATTACCGGA GTCTTTAAGC GGAAAAGTTG GCGTCGATAC TGAAGTTTTA ATTGCAGGAA AAATTAAAAG TACAATCGGG GCAATATCGC ATTACTTATC AGATAATGGT ACTTTAACAG CTAAAATTAT TGTACCTATG GGTACAAAAA TCCTGCATAA TAGCTTTGTA GATGTTATGC TTATAATTAA CCCTCATAAA AACCTAACCG TTCCTGCAAG TTGTATTCAA CGCAATAATC AAGGTAATTT TATTTATAAA ATAGACGGTT ATACGATAAA ACAATTATAT GTAAAAATCG GTACACGTAC AGAAGGTCTA ATTGAAATTA TCTCTGACGA TATTAAAGCA GGTGACTTAG TCGTTACCGA AGGTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT