SSGCID: Target Page

RiriA.18411.a

VirB10 protein

CENTER ID:	RiriA.18411.a
ORGANISM:	Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE:	Rocky Mountain Spotted fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiriA.18411.a.B2.GE40002	soluble domain		58	482
RiriA.18411.a.B3.GE40003	globular domain (aln H.pyl struc)		190	482

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiriA.18411.a.B3.PS38270	globular domain (aln H.pyl struc)		190	482

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|392021.5.peg.468
RefSeq:	WP_012150600.1
UniProt:	A0A0H3AU55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEEQNNNHN TGALSGADSP EVQRELSKVA VSFNKSIAIV VVICGIFIYI FYTLFFGTEK EEIPETQIPS NIVKPVMEVD DNIPEIPKLP DPPKLETPTA PPPPLPPVVK VPPVLPPTTP VEENKDKTLP LPPVSLPSTS GTLVESDAEK QRREAKRKSA IVLIGGVEPK KTPEQITAET TFKDRGDMSL VLGRGKLIDA VLETAINSEL GGEIRAIISR DVFSEKDKVI LIPKGSKIFG KYATSTSSNS YGRVSIIWDR IDLTSGYTIE FDSPAVDNLG RPGLQGRVDN KYKEQFANAV LQSGFNIGLA KVLDKLVPPP IASQAAATNS ATAKQLLNTA QTIASNTAMD ANTRIVTICT NILAAITDKT STAYTTMTQA CTTAQTASSA NTAEQRLQTL VQAVNTAASS LLTTTSIAST PTQAQQASTQ AFTDVTHVVK NMITQEQFKP TTTVNQGTPV RIYVNKDYKF PKAVLLKSKV MK

NT Sequence

atggctgaag agcaaaataa caatcataat accggtgctt tatcaggagc agattctcct gaagtacaaa gagaattatc aaaagttgcg gttagcttta ataagagtat tgcgatagta gtcgtaattt gtggtatttt tatatatatt ttttatactc tttttttcgg tactgaaaaa gaagaaatac cggagactca gataccgagt aatattgtaa aacctgttat ggaggttgat gataatattc cggagattcc aaagttacct gatccgccta agcttgagac gcccaccgcg cctccaccgc ctctccctcc ggtagtaaaa gttccgccgg tcttacctcc tactactcct gtagaagaga ataaagacaa aacgcttcca ttgccgccgg tttcgttgcc ttcgacttca ggaacgttag ttgagagcga tgcagagaag caacgtcgtg aagcaaaaag aaaatcagct atagtgttaa ttggtggtgt agagcctaaa aaaacaccgg aacagattac agcagaaacg acctttaaag atcgcggtga tatgtctttg gttctcggac gcggtaaatt aattgatgca gtccttgaga cggcaataaa tagtgagctc ggcggtgaaa taagagccat tattagtaga gacgtatttt ctgagaaaga taaagtgata ttgataccga aagggtcaaa aatattcggt aaatatgcaa cctcaacttc gtccaatagt tatggtagag tttctataat atgggataga atcgatttaa cgagcggtta tactatagaa ttcgactcac ctgcagttga taatttaggt agaccgggat tacagggaag agttgataat aaatataaag aacaatttgc aaatgccgta ttacaatccg gctttaatat agggcttgct aaagtccttg ataagttagt gccgcctcct atagcgtctc aggcagcggc taccaatagt gctacggcaa aacaattgtt aaatactgct caaactattg cgtctaatac cgctatggat gcaaatacta ggatagttac aatttgtact aatatacttg ctgctattac tgataaaact tcgactgctt atactactat gactcaagca tgtacaaccg cacaaactgc ttcttcagcg aatactgctg agcagagact tcaaacctta gtacaggcag ttaatacggc agcttcaagt ttgcttacta ctacctctat agcatcaact ccgacgcaag cacaacaagc ttctactcaa gcttttacgg atgtaacgca tgttgtaaaa aatatgataa ctcaggagca gtttaagccg acgacaacgg ttaatcaagg tacgccggtt agaatatacg ttaataagga ttataaattc ccaaaagccg ttttattaaa atcgaaggta atgaaatga

Details for RiriA.18411.a.B2.GE40002

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	47kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	93
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMT EKEEIPETQI PSNIVKPVME VDDNIPEIPK LPDPPKLETP TAPPPPLPPV VKVPPVLPPT TPVEENKDKT LPLPPVSLPS TSGTLVESDA EKQRREAKRK SAIVLIGGVE PKKTPEQITA ETTFKDRGDM SLVLGRGKLI DAVLETAINS ELGGEIRAII SRDVFSEKDK VILIPKGSKI FGKYATSTSS NSYGRVSIIW DRIDLTSGYT IEFDSPAVDN LGRPGLQGRV DNKYKEQFAN AVLQSGXNIG LAKVLDKLVX PPIASQAAAT NXXXXKQXXX XAQXXXXNTA MDANTRIVTI CTNILAAITD KTXXXXXTMT QACTXXQTAS SXNXXEQRLQ TLVQAVNTAA SSLLTTTSIA STPTQAQQAS TQAFTDVTHV VKNMITQEQF KPTTTVNQGT PVRIYVNKDY KFPKXX

Validated NT Sequence

tannnnnnct tttgggaatt tataatcctt attaacgtat attctaaccg gcgtaccttg attaaccgtt gtcgtcggct taaactgctc ctgagttatc atatttttta caacatgcgt tacatccgta aaagcttgag tagaagcttg ttgtgcttgc gtcggagttg atgctataga ggtagtagta agcaaacttg aagctgccgt attaactgcc tgtactaagg tttgaagtct ctgctcnnnn nnattcgntg aagaagcagt ttgtgnnntt gtacatgctt gagtcatagt annntannnn nnnnaagttt tatcagtaat agcagcaagt atattagtac aaattgtaac tatcctngta tttgcatcca tagcggtatt annnnnnnnn nnttgagcnn nnntnnnnna ttgttttgnn nnnnnnctat tggtagccgc tgcctgagac gctataggag gcgncactaa cttatcaagg actttagcaa gccctatatt naagccggat tgtaatacgg catttgcaaa ttgttcttta tatttattat caactcttcc ctgtaatccc ggtctaccta aattatcaac tgcaggtgag tcgaattcta tagtataacc gctcgttaaa tcgattctat cccatattat agaaactcta ccataactat tggacgaagt tgaggttgca tatttaccga atatttttga ccctttcggt atcaatatca ctttatcttt ctcagaaaat acgtctctac taataatggc tcttatttca ccgccgagct cactatttat tgccgtctca aggactgcat caattaattt accgcgtccg agaaccaaag acatatcacc gcgatcttta aaggtcgttt ctgctgtaat ctgttccggt gtttttttag gctctacacc accaattaac actatagctg attttctttt tgcttcacga cgttgcttct ctgcatcgct ctcaactaac gttcctgaag tcgaaggcaa cgaaaccggc ggcaatggaa gcgttttgtc tttattctct tctacaggag tagtaggagg taagaccggc ggaactttta ctaccggagg gagaggcggt ggaggcgcgg tgggcgtctc aagcttaggc ggatcaggta actttggaat ctccggaata ttatcatcaa cctccataac aggttttaca atattactcg gtatctgagt ctccggtatt tcttcttttt cagtcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHTE KEEIPETQIP SNIVKPVMEV DDNIPEIPKL PDPPKLETPT APPPPLPPVV KVPPVLPPTT PVEENKDKTL PLPPVSLPST SGTLVESDAE KQRREAKRKS AIVLIGGVEP KKTPEQITAE TTFKDRGDMS LVLGRGKLID AVLETAINSE LGGEIRAIIS RDVFSEKDKV ILIPKGSKIF GKYATSTSSN SYGRVSIIWD RIDLTSGYTI EFDSPAVDNL GRPGLQGRVD NKYKEQFANA VLQSGFNIGL AKVLDKLVPP PIASQAAATN SATAKQLLNT AQTIASNTAM DANTRIVTIC TNILAAITDK TSTAYTTMTQ ACTTAQTASS ANTAEQRLQT LVQAVNTAAS SLLTTTSIAS TPTQAQQAST QAFTDVTHVV KNMITQEQFK PTTTVNQGTP VRIYVNKDYK FPKAVLLKSK VMK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catactgaaa aagaagaaat accggagact cagataccga gtaatattgt aaaacctgtt atggaggttg atgataatat tccggagatt ccaaagttac ctgatccgcc taagcttgag acgcccaccg cgcctccacc gcctctccct ccggtagtaa aagttccgcc ggtcttacct cctactactc ctgtagaaga gaataaagac aaaacgcttc cattgccgcc ggtttcgttg ccttcgactt caggaacgtt agttgagagc gatgcagaga agcaacgtcg tgaagcaaaa agaaaatcag ctatagtgtt aattggtggt gtagagccta aaaaaacacc ggaacagatt acagcagaaa cgacctttaa agatcgcggt gatatgtctt tggttctcgg acgcggtaaa ttaattgatg cagtccttga gacggcaata aatagtgagc tcggcggtga aataagagcc attattagta gagacgtatt ttctgagaaa gataaagtga tattgatacc gaaagggtca aaaatattcg gtaaatatgc aacctcaact tcgtccaata gttatggtag agtttctata atatgggata gaatcgattt aacgagcggt tatactatag aattcgactc acctgcagtt gataatttag gtagaccggg attacaggga agagttgata ataaatataa agaacaattt gcaaatgccg tattacaatc cggctttaat atagggcttg ctaaagtcct tgataagtta gtgccgcctc ctatagcgtc tcaggcagcg gctaccaata gtgctacggc aaaacaattg ttaaatactg ctcaaactat tgcgtctaat accgctatgg atgcaaatac taggatagtt acaatttgta ctaatatact tgctgctatt actgataaaa cttcgactgc ttatactact atgactcaag catgtacaac cgcacaaact gcttcttcag cgaatactgc tgagcagaga cttcaaacct tagtacaggc agttaatacg gcagcttcaa gtttgcttac tactacctct atagcatcaa ctccgacgca agcacaacaa gcttctactc aagcttttac ggatgtaacg catgttgtaa aaaatatgat aactcaggag cagtttaagc cgacgacaac ggttaatcaa ggtacgccgg ttagaatata cgttaataag gattataaat tcccaaaagc cgttttatta aaatcgaagg taatgaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for RiriA.18411.a.B3.GE40003

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	32kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHML VLGRGKLIDA VLETAINSEL GGEIRAIISR DVFSEKDKVI LIPKGSKIFG KYATSTSSNS YGRVSIIWDR IDLTSGYTIE FDSPAVDNLG RPGLQGRVDN KYKEQFANAV LQSGFNIGLA KVLDKLVPPP IASQAAATNS ATAKQLLNTA QTIASNTAMD ANTRIVTICT NILAAITDKT STAYTTMTQA CTTAQTASSA NTAEQRLQTL VQAVNTAASS LLTTTSIAST PTQAQQASTQ AFTDVTHVVK NMITQEQFKP TTTVNQGTPV RIYVNKDYKF PKAXXXNRR

Validated NT Sequence

atcttactca cttatttcat taccttcgat ttnnnnnnnn ngcttttggg aatttataat ccttattaac gtatattcta accggcgtac cttgattaac cgttgtcgtc ggcttaaact gctcctgagt tatcatattt tttacaacat gcgttacatc cgtaaaagct tgagtagaag cttgttgtgc ttgcgtcgga gttgatgcta tagaggtagt agtaagcaaa cttgaagctg ccgtattaac tgcctgtact aaggtttgaa gtctctgctc agcagtattc gctgaagaag cagtttgtgc ggttgtacat gcttgagtca tagtagtata agcagtcgaa gttttatcag taatagcagc aagtatatta gtacaaattg taactatcct agtatttgca tccatagcgg tattagacgc aatagtttga gcagtattta acaattgttt tgccgtagca ctattggtag ccgctgcctg agacgctata ggaggcggca ctaacttatc aaggacttta gcaagcccta tattaaagcc ggattgtaat acggcatttg caaattgttc tttatattta ttatcaactc ttccctgtaa tcccggtcta cctaaattat caactgcagg tgagtcgaat tctatagtat aaccgctcgt taaatcgatt ctatcccata ttatagaaac tctaccataa ctattggacg aagttgaggt tgcatattta ccgaatattt ttgacccttt cggtatcaat atcactttat ctttctcaga aaatacgtct ctactaataa tggctcttat ttcaccgccg agctcactat ttattgccgt ctcaaggact gcatcaatta atttaccgcg tccgagaacc aacatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHLV LGRGKLIDAV LETAINSELG GEIRAIISRD VFSEKDKVIL IPKGSKIFGK YATSTSSNSY GRVSIIWDRI DLTSGYTIEF DSPAVDNLGR PGLQGRVDNK YKEQFANAVL QSGFNIGLAK VLDKLVPPPI ASQAAATNSA TAKQLLNTAQ TIASNTAMDA NTRIVTICTN ILAAITDKTS TAYTTMTQAC TTAQTASSAN TAEQRLQTLV QAVNTAASSL LTTTSIASTP TQAQQASTQA FTDVTHVVKN MITQEQFKPT TTVNQGTPVR IYVNKDYKFP KAVLLKSKVM K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggttc tcggacgcgg taaattaatt gatgcagtcc ttgagacggc aataaatagt gagctcggcg gtgaaataag agccattatt agtagagacg tattttctga gaaagataaa gtgatattga taccgaaagg gtcaaaaata ttcggtaaat atgcaacctc aacttcgtcc aatagttatg gtagagtttc tataatatgg gatagaatcg atttaacgag cggttatact atagaattcg actcacctgc agttgataat ttaggtagac cgggattaca gggaagagtt gataataaat ataaagaaca atttgcaaat gccgtattac aatccggctt taatataggg cttgctaaag tccttgataa gttagtgccg cctcctatag cgtctcaggc agcggctacc aatagtgcta cggcaaaaca attgttaaat actgctcaaa ctattgcgtc taataccgct atggatgcaa atactaggat agttacaatt tgtactaata tacttgctgc tattactgat aaaacttcga ctgcttatac tactatgact caagcatgta caaccgcaca aactgcttct tcagcgaata ctgctgagca gagacttcaa accttagtac aggcagttaa tacggcagct tcaagtttgc ttactactac ctctatagca tcaactccga cgcaagcaca acaagcttct actcaagctt ttacggatgt aacgcatgtt gtaaaaaata tgataactca ggagcagttt aagccgacga caacggttaa tcaaggtacg ccggttagaa tatacgttaa taaggattat aaattcccaa aagccgtttt attaaaatcg aaggtaatga aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for RiriA.18411.a.B3.PS38270

PURIFICATION DATe:	5/2/2017
CONCENTRATION:	18.7mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer: 20mM HEPES pH 7.0, 300mM NaCl, 5% glycerol, 1mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGTTC TCGGACGCGG TAAATTAATT GATGCAGTCC TTGAGACGGC AATAAATAGT GAGCTCGGCG GTGAAATAAG AGCCATTATT AGTAGAGACG TATTTTCTGA GAAAGATAAA GTGATATTGA TACCGAAAGG GTCAAAAATA TTCGGTAAAT ATGCAACCTC AACTTCGTCC AATAGTTATG GTAGAGTTTC TATAATATGG GATAGAATCG ATTTAACGAG CGGTTATACT ATAGAATTCG ACTCACCTGC AGTTGATAAT TTAGGTAGAC CGGGATTACA GGGAAGAGTT GATAATAAAT ATAAAGAACA ATTTGCAAAT GCCGTATTAC AATCCGGCTT TAATATAGGG CTTGCTAAAG TCCTTGATAA GTTAGTGCCG CCTCCTATAG CGTCTCAGGC AGCGGCTACC AATAGTGCTA CGGCAAAACA ATTGTTAAAT ACTGCTCAAA CTATTGCGTC TAATACCGCT ATGGATGCAA ATACTAGGAT AGTTACAATT TGTACTAATA TACTTGCTGC TATTACTGAT AAAACTTCGA CTGCTTATAC TACTATGACT CAAGCATGTA CAACCGCACA AACTGCTTCT TCAGCGAATA CTGCTGAGCA GAGACTTCAA ACCTTAGTAC AGGCAGTTAA TACGGCAGCT TCAAGTTTGC TTACTACTAC CTCTATAGCA TCAACTCCGA CGCAAGCACA ACAAGCTTCT ACTCAAGCTT TTACGGATGT AACGCATGTT GTAAAAAATA TGATAACTCA GGAGCAGTTT AAGCCGACGA CAACGGTTAA TCAAGGTACG CCGGTTAGAA TATACGTTAA TAAGGATTAT AAATTCCCAA AAGCCGTTTT ATTAAAATCG AAGGTAATGA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT