RiriA.17987.a
DNA polymerase III subunit beta (EC 2.7.7.7)
| CENTER ID: | RiriA.17987.a |
| ORGANISM: | Rickettsia rickettsii str. Sheila Smith |
| ASSOCIATED DISEASE: | Rocky Mountain Spotted fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiriA.17987.a.B1.GE41046 | full length | 1 | 379 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiriA.17987.a.B1.PW38222 | full length | 1 | 379 |
Structures
6DLK
DEPOSITED: 6/1/2018
DETERMINATION: XRay
CLONE: RiriA.17987.a.B1.GE41046
PROTEIN: RiriA.17987.a.B1.PW38222
6PTV
DEPOSITED: 7/16/2019
DETERMINATION: XRay
CLONE: RiriA.17987.a.B1.GE41046
PROTEIN: RiriA.17987.a.B1.PW38222
Publications by SSGCID
Exquisite selectivity of griselimycin extends to beta subunit of DNA polymerases from Gram-negative bacterial pathogens
Abendroth JA, Barrett L, Choi R, Dranow DM, Edwards TE, Hewitt SN, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Subramanian S, Udell HS, Van Voorhis W, Wallace E, Mayclin SJ, Hammerson B, Chun IKH, Barrett KF, Conrady DG, Mueller R, Tillery L, Lukat P, Blankenfeldt W, Higgins T, Bowatte K, Smutney H, Stigliano M, Fenwick MK, Craig J, Peng V, DeRocher AE
Nat Commun - 2024
volume 7, issue 1,
PMID: 39639052; PMCID: PMC11621423
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| UniProt: | A0A0H3AWV3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKLIVETKT LVQSLGFASS VVEKRNVIPE YANIKLSAQD GNLELSSTNM DLYLSQKIAV QVVSEGECTV STKTLNDIVR KLPDSELTLT DLGTTGLEIK GKNCKFNLFT LPVSSFPAMD SINPEASFKI SCTDFAKIIE STKFSISLDE TRYNLNGVYL HIKDKEFCSA STDGHRLSIS WVTLEKQIKN FGVILPQKSA EEILKIVKDP KNINEDIEIL LNSNKIKFIC NENTIMLSKL IDGTFPDYST FIPESSSSKL VINRKMFADS IERIAIITVE KFRAVKLSLS RETLEISAVG EARGNAKEVI NSSQDKESFY EYNSDESLAI GFNPQYLEDV LKAVKSDLVE LYFSDVSAPV LIKFPENPKD IFVVMPVKV
NT Sequence
atgttaaaat taatagttga aacaaaaaca ttagtgcagt ccctagggtt tgcaagttcc gttgttgaaa aacgtaatgt catacctgaa tatgcaaaca ttaaattatc ggcacaagac ggcaatctag agcttagttc tactaatatg gatttgtact taagtcaaaa aatagcagta caggtagtaa gtgaaggtga atgtaccgtt tctacgaaaa ctctaaatga tatagtccga aaacttccgg attccgagct tacattaaca gatctcggta caacgggact tgagatcaaa ggaaaaaatt gtaaatttaa tttatttact ttgccggtta gttcttttcc tgcaatggat agtattaatc ctgaagcaag ttttaaaatt tcctgcacgg attttgctaa aataattgaa tcgacaaaat tttcgatttc tttagatgaa actcgttata atttaaacgg tgtttattta cacataaaag acaaagagtt ttgttcggca agtaccgatg ggcatagact ttcgatttca tgggtaacat tagaaaaaca aataaagaat tttggagtta tattacccca aaaaagtgca gaggaaattt taaaaatcgt taaagatcct aaaaatataa atgaagatat agaaatttta ttaaatagca ataagattaa atttatatgt aacgaaaata ctattatgct atcaaagctt atagacggta ctttccctga ttatagtact tttattccgg aaagcagcag ctcaaaatta gtaattaatc gaaaaatgtt tgcagatagt atagaaagga tagcgataat aaccgttgaa aaatttagag cagtaaaatt atcgttatct cgtgaaacct tagagattag tgctgtcggt gaagcaagag gcaacgcaaa agaggttatt aattcttcac aagataagga aagtttttat gaatataata gtgatgagtc tttagctata ggctttaacc cgcaatattt ggaagatgtc ttaaaagcag tgaaatcgga tttagtagaa ctatattttt cagatgtttc agcaccggta cttattaaat ttcctgagaa tcctaaagat atttttgtcg tcatgcctgt taaggtgtaa
Details for RiriA.17987.a.B1.GE41046
| HARVESTED ON: | 1/4/2017 |
| SEQUENCED ON: | 1/5/2017 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML KLIVETKTLV QSLGFASSVV EKRNVIPEYA NIKLSAQDGN LELSSTNMDL YLSQKIAVQV VSEGECTVST KTLNDIVRKL PDSELTLTDL GTTGLEIKGK NCKFNLFTLP VSSFPAMDSI NPEASFKISC TDFAKIIEST KFSISLDETR YNLNGVYLHI KDKEFCSAST DGHRLSISWV TLEKQIKNFG VILPQKSAEE ILKIVKDPKN INEDIEILLN SNKIKFICNE NTIMLSKLID GTFPDYSTFI PESSSSKLVI NRKMFADSIE RIAIITVEKF RAVKLSLSRE TLEISAVGEA RGNAKEVINS SQDKESFYEY NSDESLAIGF NPQYLEDVLK AVKSDLVELY FSDVSAPVLI KFPENPKDIF VVLPVKV
Validated NT Sequence
aaatatcttt aggattctca ggaaatttaa taagtaccgg tgctgaaaca tctgaaaaat atagttctac taaatccgat ttcactgctt ttaagacatc ttccaaatat tgcgggttaa agcctatagc taaagactca tcactattat attcataaaa actttcctta tcttgtgaag aattaataac ctcttttgcg ttgcctcttg cttcaccgac agcactaatc tctaaggttt cacgagataa cgataatttt actgctctaa atttttcaac ggttattatc gctatccttt ctatactatc tgcaaacatt tttcgattaa ttactaattt tgagctgctg ctttccggaa taaaagtact ataatcaggg aaagtaccgt ctataagctt tgatagcata atagtatttt cgttacatat aaatttaatc ttattgctat ttaataaaat ttctatatct tcatttatat ttttaggatc tttaacgatt tttaaaattt cctctgcact tttttggggt aatataactc caaaattctt tatttgtttt tctaatgtta cccatgaaat cgaaagtcta tgcccatcgg tacttgccga acaaaactct ttgtctttta tgtgtaaata aacaccgttt aaattataac gagtttcatc taaagaaatc gaaaattttg tcgattcaat tattttagca aaatccgtgc aggaaatttt aaaacttgct tcaggattaa tactatccat tgcaggaaaa gaactaaccg gcaaagtaaa taaattaaat ttacaatttt ttcctttgat ctcaagtccc gttgtaccga gatctgttaa tgtaagctcg gaatccggaa gttttcggac tatatcattt agagttttcg tagaaacggt acattcacct tcacttacta cctgtactgc tattttttga cttaagtaca aatccatatt agtagaacta agctctagat tgccgtcttg tgccgataat ttaatgtttg catattcagg tatgacatta cgtttttcaa caacggaact tgcaaaccct agggactgca ctaatgtttt tgtttcaact attaatttta acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHML KLIVETKTLV QSLGFASSVV EKRNVIPEYA NIKLSAQDGN LELSSTNMDL YLSQKIAVQV VSEGECTVST KTLNDIVRKL PDSELTLTDL GTTGLEIKGK NCKFNLFTLP VSSFPAMDSI NPEASFKISC TDFAKIIEST KFSISLDETR YNLNGVYLHI KDKEFCSAST DGHRLSISWV TLEKQIKNFG VILPQKSAEE ILKIVKDPKN INEDIEILLN SNKIKFICNE NTIMLSKLID GTFPDYSTFI PESSSSKLVI NRKMFADSIE RIAIITVEKF RAVKLSLSRE TLEISAVGEA RGNAKEVINS SQDKESFYEY NSDESLAIGF NPQYLEDVLK AVKSDLVELY FSDVSAPVLI KFPENPKDIF VVMPVKV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa aattaatagt tgaaacaaaa acattagtgc agtccctagg gtttgcaagt tccgttgttg aaaaacgtaa tgtcatacct gaatatgcaa acattaaatt atcggcacaa gacggcaatc tagagcttag ttctactaat atggatttgt acttaagtca aaaaatagca gtacaggtag taagtgaagg tgaatgtacc gtttctacga aaactctaaa tgatatagtc cgaaaacttc cggattccga gcttacatta acagatctcg gtacaacggg acttgagatc aaaggaaaaa attgtaaatt taatttattt actttgccgg ttagttcttt tcctgcaatg gatagtatta atcctgaagc aagttttaaa atttcctgca cggattttgc taaaataatt gaatcgacaa aattttcgat ttctttagat gaaactcgtt ataatttaaa cggtgtttat ttacacataa aagacaaaga gttttgttcg gcaagtaccg atgggcatag actttcgatt tcatgggtaa cattagaaaa acaaataaag aattttggag ttatattacc ccaaaaaagt gcagaggaaa ttttaaaaat cgttaaagat cctaaaaata taaatgaaga tatagaaatt ttattaaata gcaataagat taaatttata tgtaacgaaa atactattat gctatcaaag cttatagacg gtactttccc tgattatagt acttttattc cggaaagcag cagctcaaaa ttagtaatta atcgaaaaat gtttgcagat agtatagaaa ggatagcgat aataaccgtt gaaaaattta gagcagtaaa attatcgtta tctcgtgaaa ccttagagat tagtgctgtc ggtgaagcaa gaggcaacgc aaaagaggtt attaattctt cacaagataa ggaaagtttt tatgaatata atagtgatga gtctttagct ataggcttta acccgcaata tttggaagat gtcttaaaag cagtgaaatc ggatttagta gaactatatt tttcagatgt ttcagcaccg gtacttatta aatttcctga gaatcctaaa gatatttttg tcgtcatgcc tgttaaggtg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for RiriA.17987.a.B1.PW38222
| PURIFICATION DATe: | 3/13/2017 |
| CONCENTRATION: | 25mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KLIVETKTLV QSLGFASSVV EKRNVIPEYA NIKLSAQDGN LELSSTNMDL YLSQKIAVQV VSEGECTVST KTLNDIVRKL PDSELTLTDL GTTGLEIKGK NCKFNLFTLP VSSFPAMDSI NPEASFKISC TDFAKIIEST KFSISLDETR YNLNGVYLHI KDKEFCSAST DGHRLSISWV TLEKQIKNFG VILPQKSAEE ILKIVKDPKN INEDIEILLN SNKIKFICNE NTIMLSKLID GTFPDYSTFI PESSSSKLVI NRKMFADSIE RIAIITVEKF RAVKLSLSRE TLEISAVGEA RGNAKEVINS SQDKESFYEY NSDESLAIGF NPQYLEDVLK AVKSDLVELY FSDVSAPVLI KFPENPKDIF VVLPVKV
Validated NT Sequence
aaatatcttt aggattctca ggaaatttaa taagtaccgg tgctgaaaca tctgaaaaat atagttctac taaatccgat ttcactgctt ttaagacatc ttccaaatat tgcgggttaa agcctatagc taaagactca tcactattat attcataaaa actttcctta tcttgtgaag aattaataac ctcttttgcg ttgcctcttg cttcaccgac agcactaatc tctaaggttt cacgagataa cgataatttt actgctctaa atttttcaac ggttattatc gctatccttt ctatactatc tgcaaacatt tttcgattaa ttactaattt tgagctgctg ctttccggaa taaaagtact ataatcaggg aaagtaccgt ctataagctt tgatagcata atagtatttt cgttacatat aaatttaatc ttattgctat ttaataaaat ttctatatct tcatttatat ttttaggatc tttaacgatt tttaaaattt cctctgcact tttttggggt aatataactc caaaattctt tatttgtttt tctaatgtta cccatgaaat cgaaagtcta tgcccatcgg tacttgccga acaaaactct ttgtctttta tgtgtaaata aacaccgttt aaattataac gagtttcatc taaagaaatc gaaaattttg tcgattcaat tattttagca aaatccgtgc aggaaatttt aaaacttgct tcaggattaa tactatccat tgcaggaaaa gaactaaccg gcaaagtaaa taaattaaat ttacaatttt ttcctttgat ctcaagtccc gttgtaccga gatctgttaa tgtaagctcg gaatccggaa gttttcggac tatatcattt agagttttcg tagaaacggt acattcacct tcacttacta cctgtactgc tattttttga cttaagtaca aatccatatt agtagaacta agctctagat tgccgtcttg tgccgataat ttaatgtttg catattcagg tatgacatta cgtttttcaa caacggaact tgcaaaccct agggactgca ctaatgtttt tgtttcaact attaatttta acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHML KLIVETKTLV QSLGFASSVV EKRNVIPEYA NIKLSAQDGN LELSSTNMDL YLSQKIAVQV VSEGECTVST KTLNDIVRKL PDSELTLTDL GTTGLEIKGK NCKFNLFTLP VSSFPAMDSI NPEASFKISC TDFAKIIEST KFSISLDETR YNLNGVYLHI KDKEFCSAST DGHRLSISWV TLEKQIKNFG VILPQKSAEE ILKIVKDPKN INEDIEILLN SNKIKFICNE NTIMLSKLID GTFPDYSTFI PESSSSKLVI NRKMFADSIE RIAIITVEKF RAVKLSLSRE TLEISAVGEA RGNAKEVINS SQDKESFYEY NSDESLAIGF NPQYLEDVLK AVKSDLVELY FSDVSAPVLI KFPENPKDIF VVMPVKV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAA AATTAATAGT TGAAACAAAA ACATTAGTGC AGTCCCTAGG GTTTGCAAGT TCCGTTGTTG AAAAACGTAA TGTCATACCT GAATATGCAA ACATTAAATT ATCGGCACAA GACGGCAATC TAGAGCTTAG TTCTACTAAT ATGGATTTGT ACTTAAGTCA AAAAATAGCA GTACAGGTAG TAAGTGAAGG TGAATGTACC GTTTCTACGA AAACTCTAAA TGATATAGTC CGAAAACTTC CGGATTCCGA GCTTACATTA ACAGATCTCG GTACAACGGG ACTTGAGATC AAAGGAAAAA ATTGTAAATT TAATTTATTT ACTTTGCCGG TTAGTTCTTT TCCTGCAATG GATAGTATTA ATCCTGAAGC AAGTTTTAAA ATTTCCTGCA CGGATTTTGC TAAAATAATT GAATCGACAA AATTTTCGAT TTCTTTAGAT GAAACTCGTT ATAATTTAAA CGGTGTTTAT TTACACATAA AAGACAAAGA GTTTTGTTCG GCAAGTACCG ATGGGCATAG ACTTTCGATT TCATGGGTAA CATTAGAAAA ACAAATAAAG AATTTTGGAG TTATATTACC CCAAAAAAGT GCAGAGGAAA TTTTAAAAAT CGTTAAAGAT CCTAAAAATA TAAATGAAGA TATAGAAATT TTATTAAATA GCAATAAGAT TAAATTTATA TGTAACGAAA ATACTATTAT GCTATCAAAG CTTATAGACG GTACTTTCCC TGATTATAGT ACTTTTATTC CGGAAAGCAG CAGCTCAAAA TTAGTAATTA ATCGAAAAAT GTTTGCAGAT AGTATAGAAA GGATAGCGAT AATAACCGTT GAAAAATTTA GAGCAGTAAA ATTATCGTTA TCTCGTGAAA CCTTAGAGAT TAGTGCTGTC GGTGAAGCAA GAGGCAACGC AAAAGAGGTT ATTAATTCTT CACAAGATAA GGAAAGTTTT TATGAATATA ATAGTGATGA GTCTTTAGCT ATAGGCTTTA ACCCGCAATA TTTGGAAGAT GTCTTAAAAG CAGTGAAATC GGATTTAGTA GAACTATATT TTTCAGATGT TTCAGCACCG GTACTTATTA AATTTCCTGA GAATCCTAAA GATATTTTTG TCGTCATGCC TGTTAAGGTG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT