RiriA.17552.a

Guanosine pentaphosphate phosphohydrolase

CENTER ID: RiriA.17552.a
ORGANISM: Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE: Rocky Mountain Spotted fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.17552.a.B1.GE35617 Full length( RiriA.17552.a ) 1 473
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|392021.5.peg.471
RefSeq: YP_001494512.1
UniProt: A0A0H3AXN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKMRSAIID IGSNALRAVV YESDELGAPE IFNYKFRNYL TNLLNLDNLD VKHQTYLSLQ YLIHIFTKLS VTNIRCVATA ILRGHPKADE FKAIIKKRFN IDIEIISGER EAYLTAAGLI SGISDAFGIV ADLGGGSLEL AQIGNKKVGK LKSLPLGTKI IVSSNFGDVG LITKMLEEEF GAAHYPNLYL IGGALRLMSR IYMESINYPL KNLHNFEINR VEFELYLEKL SQIDKLKLSY YEQKVINYNA VLVIKAMIKV FSPEKIIISN YGLKEGVRFD SLPSHETEKD IIYERVKRLV KFDRNICKIE KYIEAVQYLL INADATTLII IELAIMLAQY NKNIDKTLRA NFVSEFILSS DIPFSHRQRL MLGIALTVTY TAKTDMHINK IAKKMISKSD YYNSHIIGYY IKIAREIDGP EFQEPSFSIK LKDDKFLQIN ASNILPKQVF EKVCERLKDI SSARKNIRYN FSD
NT Sequence
ATGAGTAAAA TGCGTTCAGC TATTATTGAT ATCGGTTCAA ATGCTTTAAG AGCTGTAGTT TATGAAAGTG ATGAGCTTGG AGCGCCGGAA ATTTTTAATT ATAAATTTAG AAATTATCTT ACAAATTTAC TTAATTTAGA TAATTTAGAC GTAAAACATC AAACATATTT ATCTCTACAA TATCTTATTC ATATTTTTAC CAAACTGTCT GTTACTAATA TTCGATGTGT TGCAACAGCT ATACTTAGGG GGCATCCTAA AGCAGATGAA TTCAAAGCTA TAATTAAAAA GAGATTCAAT ATTGATATTG AAATTATCTC AGGTGAACGT GAAGCTTATT TAACTGCTGC CGGATTAATC TCCGGTATTA GTGATGCTTT CGGTATTGTA GCTGATCTTG GAGGTGGAAG TCTTGAGCTT GCACAGATTG GAAATAAAAA AGTCGGTAAA TTAAAATCAT TGCCGCTTGG TACAAAAATT ATTGTTAGTA GCAATTTTGG TGATGTCGGG CTGATTACTA AAATGCTAGA GGAAGAATTT GGAGCTGCAC ATTATCCTAA TTTATATTTA ATTGGCGGTG CATTACGTCT AATGAGCCGT ATATACATGG AGTCTATAAA TTATCCCCTT AAAAATTTAC ATAATTTTGA AATAAATCGT GTAGAGTTTG AGTTATATTT AGAGAAATTA TCGCAAATCG ATAAATTAAA ACTGAGTTAT TACGAGCAGA AAGTCATAAA TTATAATGCA GTTTTAGTAA TAAAAGCAAT GATTAAGGTA TTTTCACCGG AAAAAATTAT TATCTCAAAT TACGGTTTAA AAGAAGGAGT AAGATTTGAC TCGTTGCCGT CTCATGAAAC AGAAAAAGAT ATTATTTATG AGAGGGTGAA GAGATTAGTA AAATTTGATA GAAATATATG TAAAATTGAA AAATATATTG AAGCAGTACA ATATCTTTTA ATTAATGCCG ATGCCACGAC TCTTATTATT ATTGAACTCG CTATAATGCT AGCACAATAT AATAAAAATA TTGATAAAAC GCTTAGAGCA AATTTTGTTT CGGAATTTAT ATTATCTTCA GACATTCCTT TTAGTCATAG ACAGCGTCTT ATGCTAGGTA TTGCCCTTAC GGTTACTTAT ACTGCTAAAA CTGACATGCA TATTAATAAA ATAGCTAAGA AAATGATTAG TAAAAGTGAT TATTACAACA GTCATATAAT CGGTTATTAT ATAAAAATTG CTAGAGAAAT TGATGGGCCA GAATTTCAAG AGCCTTCTTT TTCGATTAAA TTAAAAGACG ATAAATTTTT ACAAATTAAT GCTTCAAATA TCTTGCCTAA ACAAGTCTTT GAAAAGGTTT GTGAACGTTT AAAAGATATA AGTTCTGCTA GAAAAAATAT TAGGTATAAT TTTAGTGAT
Details for RiriA.17552.a.B1.GE35617
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMS KMRSAIIDIG SNALRAVVYE SDELGAPEIF NYKFRNYLTN LLNLDNLDVK HQTYLSLQYL IHIFTKLSVT NIRCVATAIL RGHPKADEFK AIIKKRFNID IEIISGEREA YLTAAGLISG ISDAFGIVAD LGGGSLELAQ IGNKKVGKLK SLPLGTKIIV SSNFGDVGLI TKMLEEEFGA AHYPNLYLIG GALRLMSRIY MESINYPLKN LHNFEINRVE FELYLEKLSQ IDKLKLXYYE QKVINYNAVL VIKAMIXXFX RKKLXSXXXF KRXXXFDSXX LXXXKXXXFX XXXXXXKXXX XXXXXKXXXX XXXXXXXXXX SXXXXSXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
attttgntta nctttaagaa ggagatatac catggctcac caccaccacc accatatgag taaaatgcgt tcagctatta ttgatatcgg ttcaaatgct ttaagagctg tagtttatga aagtgatgag cttggagcgc cggaaatttt taattataaa tttagaaatt atcttacaaa tttacttaat ttagataatt tagacgtaaa acatcaaaca tatttatctc tacaatatct tattcatatt tttaccaaac tgtctgttac taatattcga tgtgttgcaa cagctatact tagggggcat cctaaagcag atgaattcaa agctataatt aaaaagagat tcaatattga tattgaaatt atctcaggtg aacgtgaagc ttatttaact gctgccggat taatctccgg tattagtgat gctttcggta ttgtagctga tcttggaggt ggaagtcttg agcttgcaca gattggaaat aaaaaagtcg gtaaattaaa atcattgccg cttggtacaa aaattattgt tagtagcaat tttggtgatg tcgggctgat tactaaaatg ctagaggaag aatttggagc tgcacattat cctaatttat atttaattgg cggtgcatta cgtctaatga gccgtatata catggagtct ataaattatc cccttaaaaa tttacataat tttgaaataa atcgtgtaga gtttgagtta tatttagaga aattatcgca aatcgataaa ttaaaactga nttattacga gcagaaagtc ataaattata atgcagtttt agtaataaaa gcaatgatta nnnattttcn ccggaaaaaa ttatnatctc nnntacnntt taaaagaagn ngtanatttg actcnttncn nctcntgaan cngaaaanna nnntntttnt gnnnnggnga nnnnnnnnta aaantngntn nnaannnnnn ngnaanaaan nnnnntnanc ngnnnnnnnn nntttnnnnn cnanncnnna ctcntannnn nnnnantcnn nnnnngntnn ncnnnnnnan naaannnnnn nnnnnntnnn nnnnnnnnnn ntnnnnnnnn gnnngnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnt
Expected Protein Sequence
MAHHHHHHMS KMRSAIIDIG SNALRAVVYE SDELGAPEIF NYKFRNYLTN LLNLDNLDVK HQTYLSLQYL IHIFTKLSVT NIRCVATAIL RGHPKADEFK AIIKKRFNID IEIISGEREA YLTAAGLISG ISDAFGIVAD LGGGSLELAQ IGNKKVGKLK SLPLGTKIIV SSNFGDVGLI TKMLEEEFGA AHYPNLYLIG GALRLMSRIY MESINYPLKN LHNFEINRVE FELYLEKLSQ IDKLKLSYYE QKVINYNAVL VIKAMIKVFS PEKIIISNYG LKEGVRFDSL PSHETEKDII YERVKRLVKF DRNICKIEKY IEAVQYLLIN ADATTLIIIE LAIMLAQYNK NIDKTLRANF VSEFILSSDI PFSHRQRLML GIALTVTYTA KTDMHINKIA KKMISKSDYY NSHIIGYYIK IAREIDGPEF QEPSFSIKLK DDKFLQINAS NILPKQVFEK VCERLKDISS ARKNIRYNFS D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta aaatgcgttc agctattatt gatatcggtt caaatgcttt aagagctgta gtttatgaaa gtgatgagct tggagcgccg gaaattttta attataaatt tagaaattat cttacaaatt tacttaattt agataattta gacgtaaaac atcaaacata tttatctcta caatatctta ttcatatttt taccaaactg tctgttacta atattcgatg tgttgcaaca gctatactta gggggcatcc taaagcagat gaattcaaag ctataattaa aaagagattc aatattgata ttgaaattat ctcaggtgaa cgtgaagctt atttaactgc tgccggatta atctccggta ttagtgatgc tttcggtatt gtagctgatc ttggaggtgg aagtcttgag cttgcacaga ttggaaataa aaaagtcggt aaattaaaat cattgccgct tggtacaaaa attattgtta gtagcaattt tggtgatgtc gggctgatta ctaaaatgct agaggaagaa tttggagctg cacattatcc taatttatat ttaattggcg gtgcattacg tctaatgagc cgtatataca tggagtctat aaattatccc cttaaaaatt tacataattt tgaaataaat cgtgtagagt ttgagttata tttagagaaa ttatcgcaaa tcgataaatt aaaactgagt tattacgagc agaaagtcat aaattataat gcagttttag taataaaagc aatgattaag gtattttcac cggaaaaaat tattatctca aattacggtt taaaagaagg agtaagattt gactcgttgc cgtctcatga aacagaaaaa gatattattt atgagagggt gaagagatta gtaaaatttg atagaaatat atgtaaaatt gaaaaatata ttgaagcagt acaatatctt ttaattaatg ccgatgccac gactcttatt attattgaac tcgctataat gctagcacaa tataataaaa atattgataa aacgcttaga gcaaattttg tttcggaatt tatattatct tcagacattc cttttagtca tagacagcgt cttatgctag gtattgccct tacggttact tatactgcta aaactgacat gcatattaat aaaatagcta agaaaatgat tagtaaaagt gattattaca acagtcatat aatcggttat tatataaaaa ttgctagaga aattgatggg ccagaatttc aagagccttc tttttcgatt aaattaaaag acgataaatt tttacaaatt aatgcttcaa atatcttgcc taaacaagtc tttgaaaagg tttgtgaacg tttaaaagat ataagttctg ctagaaaaaa tattaggtat aattttagtg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat