RiriA.17484.a

Mrp protein

CENTER ID: RiriA.17484.a
ORGANISM: Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE: Rocky Mountain Spotted fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.17484.a.B1.GE35608 Full length( RiriA.17484.a ) 1 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.17484.a.B1.PS01598 Full length( RiriA.17484.a ) 1 319

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|392021.5.peg.198
RefSeq: YP_001494278.1
UniProt: A0A0H3AWB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANLHQRQII DKIQHITFKD GTFLNEVISD IIIKGNNIGF SIDISGKNKL EAEEIRLKAM NKLNEISEVN KITIVFTEIK PMEKKVQKPK HFVENVKKII LVASGKGGVG KSTISALIAQ QLSLANYRVG IVDADIYGPS IPHIFGINEV PQTKDGRIIP VLAQSIEIIS IGFFVKDRSA IIWRGPMASK TIYQLLSVTK WDNLDYLIID MPPGTGDIHL SILENYHLDG VIIVTTPQKI SEIDVIRSID LYQKLNLPIL GIIENMSYML KNNSGGHLSQ KYNIPLIAQI PITPQIAEAC DKSLPLTNLL TLPLEKYLQ
NT Sequence
ATGGCGAATT TACACCAACG ACAAATTATA GATAAAATTC AGCATATTAC CTTTAAAGAC GGTACTTTTT TAAATGAAGT TATATCGGAT ATTATAATTA AAGGTAATAA TATCGGTTTC TCAATAGATA TATCAGGTAA AAACAAGTTA GAAGCCGAAG AAATTAGACT TAAGGCAATG AATAAACTTA ACGAGATTTC GGAAGTAAAT AAAATAACAA TTGTTTTTAC CGAAATTAAA CCAATGGAGA AAAAAGTTCA AAAACCAAAA CATTTTGTAG AAAATGTAAA AAAAATTATC TTAGTAGCGT CAGGTAAAGG CGGAGTCGGA AAATCTACAA TATCGGCTCT TATCGCTCAG CAGTTAAGTC TAGCAAATTA CCGAGTTGGG ATAGTAGATG CGGATATTTA CGGTCCGTCA ATTCCTCATA TATTCGGCAT TAACGAAGTA CCGCAAACGA AAGATGGACG AATAATACCG GTACTTGCTC AAAGCATCGA AATTATATCT ATAGGCTTCT TCGTTAAAGA CCGCTCGGCA ATTATTTGGC GTGGTCCTAT GGCGAGTAAG ACTATTTATC AATTATTATC GGTAACAAAA TGGGATAATT TAGATTATTT AATTATCGAT ATGCCGCCGG GAACAGGTGA TATTCATTTA AGTATACTAG AAAATTATCA TTTAGACGGC GTAATAATCG TAACTACACC ACAAAAAATA TCAGAAATAG ACGTAATACG TTCTATAGAT TTATATCAAA AACTAAATTT ACCTATTTTG GGAATAATTG AGAATATGAG CTATATGCTT AAAAATAATA GTGGCGGACA TTTATCACAA AAATATAATA TACCACTAAT CGCTCAAATT CCGATCACAC CACAAATAGC CGAGGCTTGC GATAAATCAC TACCTCTAAC AAACTTATTA ACATTACCTT TAGAGAAGTA TTTACAA
Details for RiriA.17484.a.B1.GE35608
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMA NLHQRQIIDK IQHITFKDGT FLNEVISDII IKGNNIGFSI DISGKNKLEA EEIRLKAMNK LNEISEVNKI TIVFTEIKPM EKKVQKPKHF VENVKKIILV ASGKGGVGKS TISALIAQQL SLANYRVGIV DADIYGPSIP HIFGINEVPQ TKDGRIIPVL AQSIEIISIG FFVKDRSAII WRGPMASKTI YQLLSVTKWD NLDYLIIDMP PGTGDIHLSI LENYHLDGVI IVTTPQKISE IDVIRSIDLY QKLNLPILGI IENMSYMLKN NSGGHLSQKY NIPLIAQIPI TPQIAEACDK SLPLTNL
Validated NT Sequence
ataagtttgt tagaggtagt gatttatcgc aagcctcggc tatttgtggt gtgatcggaa tttgagcgat tagtggtata ttatattttt gtgataaatg tccgccacta ttatttttaa gcatatagct catattctca attattccca aaataggtaa atttagtttt tgatataaat ctatagaacg tattacgtct atttctgata ttttttgtgg tgtagttacg attattacgc cgtctaaatg ataattttct agtatactta aatgaatatc acctgttccc ggcggcatat cgataattaa ataatctaaa ttatcccatt ttgttaccga taataattga taaatagtct tactcgccat aggaccacgc caaataattg ccgagcggtc tttaacgaag aagcctatag atataatttc gatgctttga gcaagtaccg gtattattcg tccatctttc gtttgcggta cttcgttaat gccgaatata tgaggaattg acggaccgta aatatccgca tctactatcc caactcggta atttgctaga cttaactgct gagcgataag agccgatatt gtagattttc cgactccgcc tttacctgac gctactaaga taattttttt tacattttct acaaaatgtt ttggtttttg aacttttttc tccattggtt taatttcggt aaaaacaatt gttattttat ttacttccga aatctcgtta agtttattca ttgccttaag tctaatttct tcggcttcta acttgttttt acctgatata tctattgaga aaccgatatt attaccttta attataatat ccgatataac ttcatttaaa aaagtaccgt ctttaaaggt aatatgctga attttatcta taatttgtcg ttggtgtaaa ttcgccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMA NLHQRQIIDK IQHITFKDGT FLNEVISDII IKGNNIGFSI DISGKNKLEA EEIRLKAMNK LNEISEVNKI TIVFTEIKPM EKKVQKPKHF VENVKKIILV ASGKGGVGKS TISALIAQQL SLANYRVGIV DADIYGPSIP HIFGINEVPQ TKDGRIIPVL AQSIEIISIG FFVKDRSAII WRGPMASKTI YQLLSVTKWD NLDYLIIDMP PGTGDIHLSI LENYHLDGVI IVTTPQKISE IDVIRSIDLY QKLNLPILGI IENMSYMLKN NSGGHLSQKY NIPLIAQIPI TPQIAEACDK SLPLTNLLTL PLEKYLQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga atttacacca acgacaaatt atagataaaa ttcagcatat tacctttaaa gacggtactt ttttaaatga agttatatcg gatattataa ttaaaggtaa taatatcggt ttctcaatag atatatcagg taaaaacaag ttagaagccg aagaaattag acttaaggca atgaataaac ttaacgagat ttcggaagta aataaaataa caattgtttt taccgaaatt aaaccaatgg agaaaaaagt tcaaaaacca aaacattttg tagaaaatgt aaaaaaaatt atcttagtag cgtcaggtaa aggcggagtc ggaaaatcta caatatcggc tcttatcgct cagcagttaa gtctagcaaa ttaccgagtt gggatagtag atgcggatat ttacggtccg tcaattcctc atatattcgg cattaacgaa gtaccgcaaa cgaaagatgg acgaataata ccggtacttg ctcaaagcat cgaaattata tctataggct tcttcgttaa agaccgctcg gcaattattt ggcgtggtcc tatggcgagt aagactattt atcaattatt atcggtaaca aaatgggata atttagatta tttaattatc gatatgccgc cgggaacagg tgatattcat ttaagtatac tagaaaatta tcatttagac ggcgtaataa tcgtaactac accacaaaaa atatcagaaa tagacgtaat acgttctata gatttatatc aaaaactaaa tttacctatt ttgggaataa ttgagaatat gagctatatg cttaaaaata atagtggcgg acatttatca caaaaatata atataccact aatcgctcaa attccgatca caccacaaat agccgaggct tgcgataaat cactacctct aacaaactta ttaacattac ctttagagaa gtatttacaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for RiriA.17484.a.B1.PS01598
PURIFICATION DATe: 8/14/2012
CONCENTRATION: 17mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA NLHQRQIIDK IQHITFKDGT FLNEVISDII IKGNNIGFSI DISGKNKLEA EEIRLKAMNK LNEISEVNKI TIVFTEIKPM EKKVQKPKHF VENVKKIILV ASGKGGVGKS TISALIAQQL SLANYRVGIV DADIYGPSIP HIFGINEVPQ TKDGRIIPVL AQSIEIISIG FFVKDRSAII WRGPMASKTI YQLLSVTKWD NLDYLIIDMP PGTGDIHLSI LENYHLDGVI IVTTPQKISE IDVIRSIDLY QKLNLPILGI IENMSYMLKN NSGGHLSQKY NIPLIAQIPI TPQIAEACDK SLPLTNL
Validated NT Sequence
ataagtttgt tagaggtagt gatttatcgc aagcctcggc tatttgtggt gtgatcggaa tttgagcgat tagtggtata ttatattttt gtgataaatg tccgccacta ttatttttaa gcatatagct catattctca attattccca aaataggtaa atttagtttt tgatataaat ctatagaacg tattacgtct atttctgata ttttttgtgg tgtagttacg attattacgc cgtctaaatg ataattttct agtatactta aatgaatatc acctgttccc ggcggcatat cgataattaa ataatctaaa ttatcccatt ttgttaccga taataattga taaatagtct tactcgccat aggaccacgc caaataattg ccgagcggtc tttaacgaag aagcctatag atataatttc gatgctttga gcaagtaccg gtattattcg tccatctttc gtttgcggta cttcgttaat gccgaatata tgaggaattg acggaccgta aatatccgca tctactatcc caactcggta atttgctaga cttaactgct gagcgataag agccgatatt gtagattttc cgactccgcc tttacctgac gctactaaga taattttttt tacattttct acaaaatgtt ttggtttttg aacttttttc tccattggtt taatttcggt aaaaacaatt gttattttat ttacttccga aatctcgtta agtttattca ttgccttaag tctaatttct tcggcttcta acttgttttt acctgatata tctattgaga aaccgatatt attaccttta attataatat ccgatataac ttcatttaaa aaagtaccgt ctttaaaggt aatatgctga attttatcta taatttgtcg ttggtgtaaa ttcgccatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMA NLHQRQIIDK IQHITFKDGT FLNEVISDII IKGNNIGFSI DISGKNKLEA EEIRLKAMNK LNEISEVNKI TIVFTEIKPM EKKVQKPKHF VENVKKIILV ASGKGGVGKS TISALIAQQL SLANYRVGIV DADIYGPSIP HIFGINEVPQ TKDGRIIPVL AQSIEIISIG FFVKDRSAII WRGPMASKTI YQLLSVTKWD NLDYLIIDMP PGTGDIHLSI LENYHLDGVI IVTTPQKISE IDVIRSIDLY QKLNLPILGI IENMSYMLKN NSGGHLSQKY NIPLIAQIPI TPQIAEACDK SLPLTNLLTL PLEKYLQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGA ATTTACACCA ACGACAAATT ATAGATAAAA TTCAGCATAT TACCTTTAAA GACGGTACTT TTTTAAATGA AGTTATATCG GATATTATAA TTAAAGGTAA TAATATCGGT TTCTCAATAG ATATATCAGG TAAAAACAAG TTAGAAGCCG AAGAAATTAG ACTTAAGGCA ATGAATAAAC TTAACGAGAT TTCGGAAGTA AATAAAATAA CAATTGTTTT TACCGAAATT AAACCAATGG AGAAAAAAGT TCAAAAACCA AAACATTTTG TAGAAAATGT AAAAAAAATT ATCTTAGTAG CGTCAGGTAA AGGCGGAGTC GGAAAATCTA CAATATCGGC TCTTATCGCT CAGCAGTTAA GTCTAGCAAA TTACCGAGTT GGGATAGTAG ATGCGGATAT TTACGGTCCG TCAATTCCTC ATATATTCGG CATTAACGAA GTACCGCAAA CGAAAGATGG ACGAATAATA CCGGTACTTG CTCAAAGCAT CGAAATTATA TCTATAGGCT TCTTCGTTAA AGACCGCTCG GCAATTATTT GGCGTGGTCC TATGGCGAGT AAGACTATTT ATCAATTATT ATCGGTAACA AAATGGGATA ATTTAGATTA TTTAATTATC GATATGCCGC CGGGAACAGG TGATATTCAT TTAAGTATAC TAGAAAATTA TCATTTAGAC GGCGTAATAA TCGTAACTAC ACCACAAAAA ATATCAGAAA TAGACGTAAT ACGTTCTATA GATTTATATC AAAAACTAAA TTTACCTATT TTGGGAATAA TTGAGAATAT GAGCTATATG CTTAAAAATA ATAGTGGCGG ACATTTATCA CAAAAATATA ATATACCACT AATCGCTCAA ATTCCGATCA CACCACAAAT AGCCGAGGCT TGCGATAAAT CACTACCTCT AACAAACTTA TTAACATTAC CTTTAGAGAA GTATTTACAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT