RiriA.17320.a

Putative ATPase n2B

CENTER ID: RiriA.17320.a
ORGANISM: Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE: Rocky Mountain Spotted fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.17320.a.B1.GE35607 Full length( RiriA.17320.a ) 1 350
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|392021.5.peg.337
RefSeq: YP_001494401.1
UniProt: A0A0H3ATT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVKSSTSLIR DSKQTALREE LKQLAAALNK PKSLFKFFNQ NNLESGIYLY GPVGSGKTML MNSFCEELTT PKIIIHYQNF MQEIHKSMHK LQTANQKDII PKIAKNYTKQ TKVLCIDEFE IKDITDAMII GRLFNALIQQ NIFIFITSNT SPNNLYKDGL QRESFLPFIK IINNTFYVKY LDNQHDYRFD KALRVKEARI IYPLTLENQN KLEKIITDIS DNNLAPQNIQ VLGREISFQK VHKRILVTDY NELFTRDLSY IDYVNICQNF NVIIVGNVHT IDANDTNTAV RFINFIDNAY FYKILLFMSL ENNPNKIYQG SARAAEFKRT ISRLNEMNSE SYLLNNDFKN
NT Sequence
ATGGTTAAAT CCTCTACTTC ATTAATACGC GATTCAAAAC AAACTGCTTT ACGAGAAGAA TTAAAACAGC TGGCAGCAGC ACTTAATAAG CCTAAAAGTT TATTCAAATT CTTTAATCAA AACAACTTAG AAAGCGGAAT TTATTTATAC GGTCCGGTGG GCAGCGGTAA AACTATGCTC ATGAATTCTT TTTGTGAAGA ATTAACCACA CCTAAAATAA TAATACATTA TCAGAACTTT ATGCAGGAAA TTCATAAATC TATGCATAAA TTACAAACAG CAAATCAAAA AGATATCATC CCTAAAATTG CTAAAAACTA TACTAAACAA ACTAAAGTGT TATGTATAGA TGAGTTTGAA ATTAAAGATA TTACTGATGC AATGATAATC GGTAGATTGT TTAATGCATT AATACAGCAA AATATTTTTA TTTTTATAAC TTCTAACACG AGTCCCAATA ATCTGTACAA AGACGGATTA CAACGAGAAT CTTTCTTACC TTTTATAAAG ATAATCAATA ATACATTTTA TGTTAAATAT CTAGATAATC AACATGATTA TCGATTTGAT AAAGCGTTAA GAGTTAAAGA GGCAAGGATA ATTTATCCTT TAACTTTAGA AAACCAAAAC AAACTTGAAA AAATTATCAC GGATATTAGT GATAATAATC TTGCTCCTCA AAATATACAG GTTCTAGGTA GAGAAATATC CTTTCAAAAA GTGCATAAAC GAATATTAGT AACAGATTAT AATGAATTAT TTACAAGAGA CTTAAGCTAT ATCGATTATG TTAATATTTG CCAAAATTTT AACGTTATTA TAGTGGGGAA CGTTCATACT ATTGATGCTA ACGATACAAA TACAGCCGTT AGATTTATCA ACTTTATCGA TAATGCCTAT TTCTATAAAA TACTCTTATT TATGAGCCTA GAAAATAACC CAAATAAAAT ATATCAAGGT TCAGCACGAG CCGCAGAATT TAAACGCACT ATTTCAAGAC TAAATGAAAT GAATAGCGAA TCTTATTTAT TAAATAATGA TTTTAAGAAT
Details for RiriA.17320.a.B1.GE35607
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 79
Validated AA Sequence
MLMNSFCEEL TTPKIIIHYQ NFMQEIHKSM HKLQTANQKD IIPKIAKNYT KQTKVLCIDE FEIKDITDAM IIGRLFNALI QQNIFIFITS NTSPNNLYKD GLQRESFLPF IKIINNTFYV KYLDNQHDYR FDKALRVKEA RIIYPLTLEN QNKLEKIITD ISDNNLAPQN IQVLGREISF QKVHKRILVT DYNELFTRDL SYIDYVNICQ NFNVIIVGNV HTIDANDTNT AVRFINFIDN AYFYKILLFM SLENNPNKIY QGSARAAEFK RTISRLNEMN SES
Validated NT Sequence
aagattcgct attcatttca tttagtcttg aaatagtgcg tttaaattct gcggctcgtg ctgaaccttg atatatttta tttgggttat tttctaggct cataaataag agtattttat agaaataggc attatcgata aagttgataa atctaacggc tgtatttgta tcgttagcat caatagtatg aacgttcccc actataataa cgttaaaatt ttggcaaata ttaacataat cgatatagct taagtctctt gtaaataatt cattataatc tgttactaat attcgtttat gcactttttg aaaggatatt tctctaccta gaacctgtat attttgagga gcaagattat tatcactaat atccgtgata attttttcaa gtttgttttg gttttctaaa gttaaaggat aaattatcct tgcctcttta actcttaacg ctttatcaaa tcgataatca tgttgattat ctagatattt aacataaaat gtattattga ttatctttat aaaaggtaag aaagattctc gttgtaatcc gtctttgtac agattattgg gactcgtgtt agaagttata aaaataaaaa tattttgctg tattaatgca ttaaacaatc taccgattat cattgcatca gtaatatctt taatttcaaa ctcatctata cataacactt tagtttgttt agtatagttt ttagcaattt tagggatgat atctttttga tttgctgttt gtaatttatg catagattta tgaatttcct gcataaagtt ctgataatgt attattattt taggtgtggt taattcttca caaaaagaat tcatgagcat agttttaccg ctgcccaccg gaccgtataa ataaattccg ctttctaagt tgttttgatt aaagaatttg aataaacttt taggcttatt aagtgctgct gccagctgtt ttaattcttc tcgtaaagca gtttgttttg aatcgcgtat taatgaagta gagga
Expected Protein Sequence
MAHHHHHHMV KSSTSLIRDS KQTALREELK QLAAALNKPK SLFKFFNQNN LESGIYLYGP VGSGKTMLMN SFCEELTTPK IIIHYQNFMQ EIHKSMHKLQ TANQKDIIPK IAKNYTKQTK VLCIDEFEIK DITDAMIIGR LFNALIQQNI FIFITSNTSP NNLYKDGLQR ESFLPFIKII NNTFYVKYLD NQHDYRFDKA LRVKEARIIY PLTLENQNKL EKIITDISDN NLAPQNIQVL GREISFQKVH KRILVTDYNE LFTRDLSYID YVNICQNFNV IIVGNVHTID ANDTNTAVRF INFIDNAYFY KILLFMSLEN NPNKIYQGSA RAAEFKRTIS RLNEMNSESY LLNNDFKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtta aatcctctac ttcattaata cgcgattcaa aacaaactgc tttacgagaa gaattaaaac agctggcagc agcacttaat aagcctaaaa gtttattcaa attctttaat caaaacaact tagaaagcgg aatttattta tacggtccgg tgggcagcgg taaaactatg ctcatgaatt ctttttgtga agaattaacc acacctaaaa taataataca ttatcagaac tttatgcagg aaattcataa atctatgcat aaattacaaa cagcaaatca aaaagatatc atccctaaaa ttgctaaaaa ctatactaaa caaactaaag tgttatgtat agatgagttt gaaattaaag atattactga tgcaatgata atcggtagat tgtttaatgc attaatacag caaaatattt ttatttttat aacttctaac acgagtccca ataatctgta caaagacgga ttacaacgag aatctttctt accttttata aagataatca ataatacatt ttatgttaaa tatctagata atcaacatga ttatcgattt gataaagcgt taagagttaa agaggcaagg ataatttatc ctttaacttt agaaaaccaa aacaaacttg aaaaaattat cacggatatt agtgataata atcttgctcc tcaaaatata caggttctag gtagagaaat atcctttcaa aaagtgcata aacgaatatt agtaacagat tataatgaat tatttacaag agacttaagc tatatcgatt atgttaatat ttgccaaaat tttaacgtta ttatagtggg gaacgttcat actattgatg ctaacgatac aaatacagcc gttagattta tcaactttat cgataatgcc tatttctata aaatactctt atttatgagc ctagaaaata acccaaataa aatatatcaa ggttcagcac gagccgcaga atttaaacgc actatttcaa gactaaatga aatgaatagc gaatcttatt tattaaataa tgattttaag aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat