RiriA.17295.a

Glycosyltransferase

CENTER ID: RiriA.17295.a
ORGANISM: Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE: Rocky Mountain Spotted fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.17295.a.B1.GE35600 Full length( RiriA.17295.a ) 1 390
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|392021.5.peg.554
RefSeq: YP_001494580.1
UniProt: A0A0H3AX38

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSLKSSKRY NKYTILQVVP ALVSGGVERG TIEVAKYLKI LGHTPIIISA GGTLVKELDK EDILHIEMNS NSKNPFVILN NAKLIAEIIK KYNVDIVHTR SRAPAWSSYL ATKWTNAKFL TTFHGVYNIP NSFKKYYNSI MLKGKKVVAV SNFVKQHLLE NYKIDEDKIV VIERGVNCDY FDPANLTPEK LEKCCDKYDV PSNVPIILMP SRMTSWKGHL VLVEALSKLK HRDFYCLMVG DISRHPNFTN RVKELIANLK LQNKIQIFGN DSDIINLYGI SDIIISASIE PEAFGRTIIE GQAMKKLVIA TNIGGAVETI KNNITGFHVE PNNAEALAQK IDYCFSILGT DLAKKIQEAA RHTVINNFSL DLMLRKNLEI YKEILKNSHN
NT Sequence
ATGCCTTCAT TAAAGTCAAG TAAACGATAC AATAAATATA CTATTTTGCA AGTAGTGCCT GCTCTAGTTT CGGGAGGAGT TGAAAGAGGT ACTATAGAAG TTGCAAAATA TCTTAAAATT CTCGGTCATA CTCCTATAAT AATTTCAGCA GGCGGTACTC TGGTTAAAGA GTTAGATAAG GAAGATATAT TGCATATTGA AATGAATAGT AACAGTAAAA ATCCTTTTGT AATTCTGAAT AATGCAAAAT TGATAGCAGA AATAATCAAA AAATATAATG TTGATATAGT TCATACAAGA TCAAGAGCAC CGGCATGGAG TTCATATCTA GCAACAAAAT GGACAAATGC TAAATTTTTG ACTACCTTTC ATGGGGTTTA TAATATTCCG AATAGTTTTA AAAAATATTA TAATAGCATA ATGCTAAAAG GCAAGAAAGT TGTTGCCGTG TCTAATTTTG TAAAACAACA CTTGCTCGAA AACTATAAAA TTGATGAAGA TAAAATAGTA GTAATTGAGC GTGGAGTGAA TTGTGATTAT TTCGATCCCG CAAATTTAAC GCCGGAAAAA CTTGAAAAAT GTTGTGATAA ATATGATGTA CCGAGTAACG TGCCGATAAT ATTAATGCCT TCTAGGATGA CAAGTTGGAA AGGACATCTT GTTCTAGTAG AAGCATTAAG TAAATTAAAA CATAGAGACT TTTATTGTTT AATGGTCGGT GATATATCTA GACATCCCAA TTTTACTAAT AGAGTAAAAG AGCTTATAGC TAATCTAAAA CTTCAAAATA AAATTCAAAT TTTTGGTAAT GATTCGGATA TAATAAACCT ATATGGGATT TCCGATATTA TCATTTCTGC CTCAATCGAG CCTGAAGCTT TCGGGCGTAC TATTATAGAA GGGCAGGCGA TGAAGAAGCT TGTTATTGCA ACTAATATCG GCGGGGCAGT AGAGACAATA AAGAATAATA TAACCGGTTT CCACGTAGAA CCAAATAATG CCGAAGCGTT AGCACAGAAA ATCGATTATT GTTTCTCGAT TTTAGGTACT GATCTTGCTA AGAAAATCCA AGAAGCGGCA CGACATACGG TAATTAATAA TTTCTCTCTT GATCTGATGC TAAGAAAAAA CCTTGAAATC TATAAAGAAA TCTTAAAAAA CTCCCATAAT
Details for RiriA.17295.a.B1.GE35600
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 78
Validated AA Sequence
MNSNSKNPFV ILNNAKLIAE IIKKYNVDIV HTRSRAPAWS SYLATKWTNA KFLTTFHGVY NIPNSFKKYY NSIMLKGKKV VAVSNFVKQH LLENYKIDED KIVVIERGVN CDYFDPANLT PEKLEKCCDK YDVPSNVPII LMPSRMTSWK GHLVLVEALS KLKHRDFYCL MVGDISRHPN FTNRVKELIA NLKLQNKIQI FGNDSDIINL YGISDIIISA SIEPEAFGRT IIEGQAMKKL VIATNIGGAV ETIKNNITGF HVEPNNAEAL AQKIDYCFSI LGTDLAKKIQ EAARHTVINN FSLDLMLRKN LEI
Validated NT Sequence
tagatttcaa ggttttttct tagcatcaga tcaagagaga aattattaat taccgtatgt cgtgccgctt cttggatttt cttagcaaga tcagtaccta aaatcgagaa acaataatcg attttctgtg ctaacgcttc ggcattattt ggttctacgt ggaaaccggt tatattattc tttattgtct ctactgcccc gccgatatta gttgcaataa caagcttctt catcgcctgc ccttctataa tagtacgccc gaaagcttca ggctcgattg aggcagaaat gataatatcg gaaatcccat ataggtttat tatatccgaa tcattaccaa aaatttgaat tttattttga agttttagat tagctataag ctcttttact ctattagtaa aattgggatg tctagatata tcaccgacca ttaaacaata aaagtctcta tgttttaatt tacttaatgc ttctactaga acaagatgtc ctttccaact tgtcatccta gaaggcatta atattatcgg cacgttactc ggtacatcat atttatcaca acatttttca agtttttccg gcgttaaatt tgcgggatcg aaataatcac aattcactcc acgctcaatt actactattt tatcttcatc aattttatag ttttcgagca agtgttgttt tacaaaatta gacacggcaa caactttctt gccttttagc attatgctat tataatattt tttaaaacta ttcggaatat tataaacccc atgaaaggta gtcaaaaatt tagcatttgt ccattttgtt gctagatatg aactccatgc cggtgctctt gatcttgtat gaactatatc aacattatat tttttgatta tttctgctat caattttgca ttattcagaa ttacaaaagg atttttactg ttactattca tttcaatatg caatatatct tccttatcta actctttaac cagagtaccg cctgctgaaa ttattatagg agtatgaccg agaattttaa gatattttgc aacttctata gtacctcttt caactcctcc cgaaactaga gcaggcacta cttgcaaaat agtatattta ttgtatcgtt tacttgactt taatgaaggc
Expected Protein Sequence
MAHHHHHHMP SLKSSKRYNK YTILQVVPAL VSGGVERGTI EVAKYLKILG HTPIIISAGG TLVKELDKED ILHIEMNSNS KNPFVILNNA KLIAEIIKKY NVDIVHTRSR APAWSSYLAT KWTNAKFLTT FHGVYNIPNS FKKYYNSIML KGKKVVAVSN FVKQHLLENY KIDEDKIVVI ERGVNCDYFD PANLTPEKLE KCCDKYDVPS NVPIILMPSR MTSWKGHLVL VEALSKLKHR DFYCLMVGDI SRHPNFTNRV KELIANLKLQ NKIQIFGNDS DIINLYGISD IIISASIEPE AFGRTIIEGQ AMKKLVIATN IGGAVETIKN NITGFHVEPN NAEALAQKID YCFSILGTDL AKKIQEAARH TVINNFSLDL MLRKNLEIYK EILKNSHN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctt cattaaagtc aagtaaacga tacaataaat atactatttt gcaagtagtg cctgctctag tttcgggagg agttgaaaga ggtactatag aagttgcaaa atatcttaaa attctcggtc atactcctat aataatttca gcaggcggta ctctggttaa agagttagat aaggaagata tattgcatat tgaaatgaat agtaacagta aaaatccttt tgtaattctg aataatgcaa aattgatagc agaaataatc aaaaaatata atgttgatat agttcataca agatcaagag caccggcatg gagttcatat ctagcaacaa aatggacaaa tgctaaattt ttgactacct ttcatggggt ttataatatt ccgaatagtt ttaaaaaata ttataatagc ataatgctaa aaggcaagaa agttgttgcc gtgtctaatt ttgtaaaaca acacttgctc gaaaactata aaattgatga agataaaata gtagtaattg agcgtggagt gaattgtgat tatttcgatc ccgcaaattt aacgccggaa aaacttgaaa aatgttgtga taaatatgat gtaccgagta acgtgccgat aatattaatg ccttctagga tgacaagttg gaaaggacat cttgttctag tagaagcatt aagtaaatta aaacatagag acttttattg tttaatggtc ggtgatatat ctagacatcc caattttact aatagagtaa aagagcttat agctaatcta aaacttcaaa ataaaattca aatttttggt aatgattcgg atataataaa cctatatggg atttccgata ttatcatttc tgcctcaatc gagcctgaag ctttcgggcg tactattata gaagggcagg cgatgaagaa gcttgttatt gcaactaata tcggcggggc agtagagaca ataaagaata atataaccgg tttccacgta gaaccaaata atgccgaagc gttagcacag aaaatcgatt attgtttctc gattttaggt actgatcttg ctaagaaaat ccaagaagcg gcacgacata cggtaattaa taatttctct cttgatctga tgctaagaaa aaaccttgaa atctataaag aaatcttaaa aaactcccat aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat