RiriA.10100.a

Periplasmic serine protease

CENTER ID: RiriA.10100.a
ORGANISM: Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE: Rocky Mountain Spotted fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.10100.a.B2.GE35595 Mature protein( RiriA.10100.a ) 21 508
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|392021.5.peg.201
RefSeq: YP_001494281.1
UniProt: A0A0H3ATJ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVNLKIVIVI IFLISSNVVL AKENSKSLKV AAQEENEFTE INSAPLKVSE AARYSFADIV EPLIPAVVNI STIEYVNSKS ENAEKDPLQE KVNDFLEKLN IPLNLEEVDQ TPKSVPLGSG FIIEPNGLIV TNYHVIANVD KINIKLADNT ELSAKLIGND TKTDLALLKI DSEEPLPFVE FGDSNDARVG DWVIAIGNPF GNLGGTVTSG IISSKGRDID IDTDNIVDNF IQTDAAINNG NSGGPMFNLD QKVIGVNTAI FSPLGTNIGI GFAIPSNTAK PIIERLKKDG KVSRGRLGVT IQDLTEEISE GLGLKNTRGV LVAKVQEDGP GDKAGIKTGD IIIEFADIPV KNTKKLRVII ADAPIDQEVK VKILRDKKEL ELPIKVTSDN EEVTKDSTEE TNKKEITNKE ENNLSITKNN ITFGNLTEEL RQKYTIPQDK MGIVITNIDE EESSFKIGDL ITNINQESID DISKLEELYD NAKKSDKQNI LLLIERGSSN MFVPLQVM
NT Sequence
ATGGTTAATC TGAAAATAGT TATTGTTATA ATATTTTTAA TATCAAGTAA CGTTGTTCTA GCAAAAGAAA ATAGTAAGTC TTTAAAAGTA GCAGCTCAAG AAGAGAATGA ATTTACGGAA ATAAATTCTG CTCCTTTAAA AGTAAGTGAA GCCGCTCGTT ATAGCTTTGC CGATATAGTG GAACCGTTAA TTCCTGCAGT CGTTAATATT TCAACAATAG AATATGTTAA TAGTAAATCG GAAAACGCCG AGAAAGATCC TCTGCAAGAA AAAGTTAATG ATTTTTTAGA AAAGCTTAAT ATACCGCTGA ATTTGGAAGA GGTTGATCAA ACTCCGAAAA GTGTCCCGCT TGGTTCAGGA TTTATTATTG AACCTAACGG CTTAATAGTG ACAAACTATC ATGTAATTGC AAATGTTGAT AAAATTAATA TAAAACTTGC AGATAATACC GAATTATCGG CTAAATTAAT AGGTAATGAT ACTAAAACCG ATTTAGCTCT CTTAAAAATA GATAGCGAGG AACCTCTACC TTTTGTTGAG TTTGGAGATT CAAATGATGC AAGAGTAGGA GATTGGGTTA TTGCGATCGG TAATCCGTTC GGTAATCTAG GCGGTACGGT GACAAGTGGT ATTATTTCTT CTAAAGGGCG GGATATCGAT ATAGATACGG ACAATATAGT CGATAATTTT ATTCAAACGG ATGCTGCAAT TAATAACGGT AATTCCGGTG GTCCTATGTT TAATTTGGAT CAGAAAGTAA TCGGCGTAAA TACGGCAATT TTTTCACCGC TCGGTACTAA TATAGGTATC GGTTTTGCAA TACCGTCAAA TACTGCAAAG CCTATAATCG AACGTCTTAA GAAAGATGGA AAAGTAAGTA GAGGACGCCT TGGAGTAACA ATACAAGATT TAACCGAGGA AATTTCTGAA GGGCTAGGAC TTAAAAATAC TAGGGGGGTG TTAGTAGCTA AAGTACAAGA AGACGGTCCA GGTGATAAAG CGGGAATTAA AACAGGTGAT ATAATAATAG AGTTTGCAGA TATACCGGTT AAAAATACTA AAAAATTACG TGTAATTATT GCAGATGCTC CTATTGATCA GGAAGTAAAA GTAAAAATAC TTCGTGATAA AAAGGAGCTT GAGTTACCTA TTAAAGTTAC TTCGGATAAT GAAGAGGTTA CCAAAGATTC TACAGAAGAG ACCAATAAGA AAGAAATAAC AAATAAAGAA GAAAATAATT TATCTATTAC TAAAAATAAT ATTACTTTTG GTAATTTGAC TGAAGAACTA AGACAAAAAT ATACTATTCC TCAAGATAAA ATGGGAATAG TGATAACCAA TATTGATGAA GAAGAAAGTA GCTTCAAAAT TGGTGATCTG ATAACCAATA TTAATCAGGA AAGCATAGAC GATATAAGTA AGCTAGAAGA ATTATATGAC AATGCTAAAA AATCAGATAA GCAAAATATT TTGCTCTTGA TTGAAAGGGG GAGTAGTAAT ATGTTCGTAC CATTGCAGGT TATG
Details for RiriA.10100.a.B2.GE35595
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 51
Validated AA Sequence
MFNLDQKVIG VNTAIFSPLG TNIGIGFAIP SNTAKPIIER LKKDGKVSRG RLGVTIQDLT EEISEGLGLK NTRGVLVAKV QEDGPGDKAG IKTGDIIIEF ADIPVKNTKK LRVIIADAPI DQEVKVKILR DKKELELPIK VTSDNEEVTK DSTEETNKKE ITNKEENNLS ITKNNITFGN LTEELRQKYT IPQDKMGIVI TNIDEEESSF KIGDLITNIN QESIDDISKL EELYDNAKKS DKQNILLLIE RGSSN
Validated NT Sequence
tattactact ccccctttca atcaagagca aaatattttg cttatctgat tttttagcat tgtcatataa ttcttctagc ttacttatat cgtctatgct ttcctgatta atattggtta tcagatcacc aattttgaag ctactttctt cttcatcaat attggttatc actattccca ttttatcttg aggaatagta tatttttgtc ttagttcttc agtcaaatta ccaaaagtaa tattattttt agtaatagat aaattatttt cttctttatt tgttatttct ttcttattgg tctcttctgt agaatctttg gtaacctctt cattatccga agtaacttta ataggtaact caagctcctt tttatcacga agtattttta cttttacttc ctgatcaata ggagcatctg caataattac acgtaatttt ttagtatttt taaccggtat atctgcaaac tctattatta tatcacctgt tttaattccc gctttatcac ctggaccgtc ttcttgtact ttagctacta acacccccct agtattttta agtcctagcc cttcagaaat ttcctcggtt aaatcttgta ttgttactcc aaggcgtcct ctacttactt ttccatcttt cttaagacgt tcgattatag gctttgcagt atttgacggt attgcaaaac cgatacctat attagtaccg agcggtgaaa aaattgccgt atttacgccg attactttct gatccaaatt aaacatagga ccaccggaat taccgttatt aattgcagca tccgtttgaa taaaattatc gactatattg tccgtatcta tatcgatatc ccgcccttta gaagaaataa taccacttgt caccgtaccg cctagattac cgaacggatt accgatcgca ataacccaat ctcctactct tgcatcattt gaatctccaa actcaacaaa aggtagaggt tcctcgctat ctatttttaa gagagctaaa tcggttttag tatcattacc tattaattta gccgataatt cggtattatc tgcaagtttt atattaattt tatcaacatt tgcaattaca tgatagtttg tcactattaa gccgttaggt tcaataataa atcctgaacc aagcgggaca cttttcggag tttgatcaac ctcttccaaa ttcagcggta tattaagctt ttctaaaaaa tcattaactt tttcttgcag aggatctttc tcggcgtttt ccgatttact attaacatat tctattgttg aaatattaac gactgcagga attaacggtt ccactatatc ggcaaagcta taacgagcgg cttcacttac ttttaaagga gcagaattta tttccgtaaa ttcattctct tcttgagctg ctacttttaa agacttacta ttttcttttg cca
Expected Protein Sequence
MAHHHHHHMA KENSKSLKVA AQEENEFTEI NSAPLKVSEA ARYSFADIVE PLIPAVVNIS TIEYVNSKSE NAEKDPLQEK VNDFLEKLNI PLNLEEVDQT PKSVPLGSGF IIEPNGLIVT NYHVIANVDK INIKLADNTE LSAKLIGNDT KTDLALLKID SEEPLPFVEF GDSNDARVGD WVIAIGNPFG NLGGTVTSGI ISSKGRDIDI DTDNIVDNFI QTDAAINNGN SGGPMFNLDQ KVIGVNTAIF SPLGTNIGIG FAIPSNTAKP IIERLKKDGK VSRGRLGVTI QDLTEEISEG LGLKNTRGVL VAKVQEDGPG DKAGIKTGDI IIEFADIPVK NTKKLRVIIA DAPIDQEVKV KILRDKKELE LPIKVTSDNE EVTKDSTEET NKKEITNKEE NNLSITKNNI TFGNLTEELR QKYTIPQDKM GIVITNIDEE ESSFKIGDLI TNINQESIDD ISKLEELYDN AKKSDKQNIL LLIERGSSNM FVPLQVM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa aagaaaatag taagtcttta aaagtagcag ctcaagaaga gaatgaattt acggaaataa attctgctcc tttaaaagta agtgaagccg ctcgttatag ctttgccgat atagtggaac cgttaattcc tgcagtcgtt aatatttcaa caatagaata tgttaatagt aaatcggaaa acgccgagaa agatcctctg caagaaaaag ttaatgattt tttagaaaag cttaatatac cgctgaattt ggaagaggtt gatcaaactc cgaaaagtgt cccgcttggt tcaggattta ttattgaacc taacggctta atagtgacaa actatcatgt aattgcaaat gttgataaaa ttaatataaa acttgcagat aataccgaat tatcggctaa attaataggt aatgatacta aaaccgattt agctctctta aaaatagata gcgaggaacc tctacctttt gttgagtttg gagattcaaa tgatgcaaga gtaggagatt gggttattgc gatcggtaat ccgttcggta atctaggcgg tacggtgaca agtggtatta tttcttctaa agggcgggat atcgatatag atacggacaa tatagtcgat aattttattc aaacggatgc tgcaattaat aacggtaatt ccggtggtcc tatgtttaat ttggatcaga aagtaatcgg cgtaaatacg gcaatttttt caccgctcgg tactaatata ggtatcggtt ttgcaatacc gtcaaatact gcaaagccta taatcgaacg tcttaagaaa gatggaaaag taagtagagg acgccttgga gtaacaatac aagatttaac cgaggaaatt tctgaagggc taggacttaa aaatactagg ggggtgttag tagctaaagt acaagaagac ggtccaggtg ataaagcggg aattaaaaca ggtgatataa taatagagtt tgcagatata ccggttaaaa atactaaaaa attacgtgta attattgcag atgctcctat tgatcaggaa gtaaaagtaa aaatacttcg tgataaaaag gagcttgagt tacctattaa agttacttcg gataatgaag aggttaccaa agattctaca gaagagacca ataagaaaga aataacaaat aaagaagaaa ataatttatc tattactaaa aataatatta cttttggtaa tttgactgaa gaactaagac aaaaatatac tattcctcaa gataaaatgg gaatagtgat aaccaatatt gatgaagaag aaagtagctt caaaattggt gatctgataa ccaatattaa tcaggaaagc atagacgata taagtaagct agaagaatta tatgacaatg ctaaaaaatc agataagcaa aatattttgc tcttgattga aagggggagt agtaatatgt tcgtaccatt gcaggttatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat