SSGCID: Target Page

RiriA.10014.f

Heme exporter protein A

CENTER ID:	RiriA.10014.f
ORGANISM:	Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE:	Rocky Mountain Spotted fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiriA.10014.f.B1.GE35597	Full length( RiriA.10014.f )		1	191

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|392021.5.peg.1414
RefSeq:	YP_001495302.1
UniProt:	A0A0H3AW00

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLSLHQLQFK NLFDLNITFL PSSITYIKGA NGCGKSSLLR MIAGIMQPSS GNIYYRNCNI NNIAKLYCTY IGHNLGLKSE MTVFENLKFW SEIYNSAATL YAAIHYFKLH DLLDKKCYSL SSGMQKIVAI ARLIACQSDL WLLDEVETNL SKENRALLNN LIVMKANSGG IVLLSSHLES SIKSAQILRL D

NT Sequence

ATGCTATCAC TTCATCAATT ACAATTTAAA AACTTATTTG ATCTAAATAT TACTTTTCTT CCTTCTTCTA TAACATATAT AAAAGGAGCT AACGGTTGTG GGAAAAGCTC GTTGCTACGA ATGATAGCAG GAATTATGCA ACCGAGTAGC GGTAATATCT ACTATAGAAA CTGTAATATT AACAATATAG CTAAACTTTA CTGCACTTAT ATCGGTCATA ATTTAGGTCT AAAATCAGAA ATGACTGTTT TTGAGAATCT AAAATTTTGG TCAGAAATTT ATAATTCCGC TGCAACCCTA TATGCTGCTA TTCATTACTT CAAATTACAC GACTTATTAG ATAAAAAATG CTATAGTTTG TCTAGCGGAA TGCAGAAAAT CGTAGCCATA GCAAGGCTTA TTGCTTGCCA ATCTGACTTA TGGCTACTTG ATGAAGTTGA AACTAACTTA AGCAAAGAAA ATAGGGCTTT ACTCAATAAT CTAATTGTCA TGAAAGCAAA TAGCGGTGGT ATTGTTCTTC TATCCTCTCA TCTAGAAAGC TCTATAAAAT CTGCACAGAT ATTACGGCTT GAT

Details for RiriA.10014.f.B1.GE35597

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML SLHQLQFKNL FDLNITFLPS SITYIKGANG CGKSSLLRMI AGIMQPSSGN IYYRNCNINN IAKLYCTYIG HNLGLKSEMT VFENLKFWSE IYNSAATLYA AIHYFKLHDL LDKKCYSLSS GMQKIVAIAR LIACQSDLWL LDEVETNLSK ENRALLNNLI VMKANSGGIV LLSSHLESSI KSAQILRLD

Validated NT Sequence

ttntttaact ttaagaagga ganataccat ggctcaccac caccaccacc atatgctatc acttcatcaa ttacaattta aaaacttatt tgatctaaat attacttttc ttccttcttc tataacatat ataaaaggag ctaacggttg tgggaaaagc tcgttgctac gaatgatagc aggaattatg caaccgagta gcggtaatat ctactataga aactgtaata ttaacaatat agctaaactt tactgcactt atatcggtca taatttaggt ctaaaatcag aaatgactgt ttttgagaat ctaaaatttt ggtcagaaat ttataattcc gctgcaaccc tatatgctgc tattcattac ttcaaattac acgacttatt agataaaaaa tgctatagtt tgtctagcgg aatgcagaaa atcgtagcca tagcaaggct tattgcttgc caatctgact tatggctact tgatgaagtt gaaactaact taagcaaaga aaatagggct ttactcaata atctaattgt catgaaagca aatagcggtg gtattgttct tctatcctct catctagaaa gctctataaa atctgcacag atattacggc ttgattaagt gagtaagata ggatccggct gctaacaagc ccccgaangn nnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctat cacttcatca attacaattt aaaaacttat ttgatctaaa tattactttt cttccttctt ctataacata tataaaagga gctaacggtt gtgggaaaag ctcgttgcta cgaatgatag caggaattat gcaaccgagt agcggtaata tctactatag aaactgtaat attaacaata tagctaaact ttactgcact tatatcggtc ataatttagg tctaaaatca gaaatgactg tttttgagaa tctaaaattt tggtcagaaa tttataattc cgctgcaacc ctatatgctg ctattcatta cttcaaatta cacgacttat tagataaaaa atgctatagt ttgtctagcg gaatgcagaa aatcgtagcc atagcaaggc ttattgcttg ccaatctgac ttatggctac ttgatgaagt tgaaactaac ttaagcaaag aaaatagggc tttactcaat aatctaattg tcatgaaagc aaatagcggt ggtattgttc ttctatcctc tcatctagaa agctctataa aatctgcaca gatattacgg cttgattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t