RiriA.00893.a

Thioredoxin reductase

CENTER ID: RiriA.00893.a
ORGANISM: Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE: Rocky Mountain Spotted fever
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.00893.a.B2.GE36186 Mature protein( RiriA.00893.a ) 26 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|392021.5.peg.736
RefSeq: YP_001494736.1
UniProt: A0A0H3AUN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKITTKVLII GSGPAGLSAA IYTARAALKP ILINGMQPGG QLTITTGVEN YPGFAETVQG PWLMEQMYMQ AKNVGTEIVS DYVEKVDLSK RPFKVFTGAG NEYDAESIII CTGAEAKWLG IASEQKFRGF GVSACATCDG VFFKNQEIVV VGGGNSAVEE ALYLTNHANK VTIVHRRDSF RAEKILQDRL FKNSKISVIW DHVVDEIVGS NKPKSVTGVK IQNVHTKEIS LLNCSGVFIA IGHAPNTGLF TGQIAMDDDN YIITKSGTTR TSVEGVFAAG DVQDKIYRQA VTAAGTGCMA ALEAEKFLNK
NT Sequence
ATGAAAATTA CTACTAAAGT ACTAATAATA GGTTCAGGAC CTGCGGGCTT AAGTGCCGCT ATTTATACAG CTAGAGCTGC TTTAAAACCA ATATTAATAA ATGGTATGCA GCCAGGCGGG CAGCTTACAA TTACCACCGG TGTTGAGAAC TATCCAGGAT TTGCGGAAAC GGTGCAAGGA CCTTGGCTTA TGGAGCAGAT GTATATGCAG GCTAAAAATG TCGGTACGGA AATTGTTAGT GATTACGTAG AAAAGGTGGA TTTATCAAAA AGACCTTTTA AAGTATTTAC AGGAGCAGGA AATGAATATG ACGCGGAGAG CATAATCATT TGTACCGGTG CGGAAGCAAA ATGGCTTGGT ATAGCTTCAG AGCAGAAATT TCGTGGTTTT GGTGTTTCGG CATGTGCTAC TTGCGATGGT GTCTTTTTTA AGAATCAAGA AATAGTTGTA GTAGGGGGAG GAAATAGTGC AGTAGAAGAA GCTTTATACT TAACCAATCA TGCTAACAAA GTCACTATAG TGCATAGAAG AGATAGTTTT AGAGCTGAGA AAATATTACA GGATCGATTA TTTAAAAATT CTAAAATATC AGTAATTTGG GATCATGTAG TAGATGAGAT TGTAGGTAGC AATAAACCAA AATCCGTTAC TGGAGTTAAA ATTCAAAATG TTCACACCAA AGAGATTAGT TTATTGAATT GTAGCGGTGT ATTTATAGCT ATTGGGCATG CACCGAATAC GGGGCTATTT ACCGGACAAA TTGCAATGGA TGACGATAAT TATATCATCA CGAAGTCAGG CACTACTAGA ACTAGTGTAG AAGGTGTTTT TGCTGCTGGT GACGTTCAGG ATAAAATTTA TAGACAAGCA GTAACTGCTG CAGGAACAGG CTGTATGGCG GCTCTTGAGG CTGAAAAATT TCTCAATAAA
Details for RiriA.00893.a.B2.GE36186
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA ALKPILINGM QPGGQLTITT GVENYPGFAE TVQGPWLMEQ MYMQAKNVGT EIVSDYVEKV DLSKRPFKVF TGAGNEYDAE SIIICTGAEA KWLGIASEQK FRGFGVSACA TCDGVFFKNQ EIVVVGGGNS AVEEALYLTN HANKVTIVHR RDSFRAEKIL QDRLFKNSKI SVIWDHVVDE IVGSNKPKSV TGVKIQNVHT KEISLLNCSG VFIAIGHAPN TGLFTGQIAM DDDXYIITKS GTTRTSVEXV FAAGDVXKIY RQAVTAAGTG CMAAXXLKIS X
Validated NT Sequence
tttgtttact ttaagangga ganataccat ggctcaccac caccaccacc atatggctgc tttaaaacca atattaataa atggtatgca gccaggcggg cagcttacaa ttaccaccgg tgttgagaac tatccaggat ttgcggaaac ggtgcaagga ccttggctta tggagcagat gtatatgcag gctaaaaatg tcggtacgga aattgttagt gattacgtag aaaaggtgga tttatcaaaa agacctttta aagtatttac aggagcagga aatgaatatg acgcggagag cataatcatt tgtaccggtg cggaagcaaa atggcttggt atagcttcag agcagaaatt tcgtggtttt ggtgtttcgg catgtgctac ttgcgatggt gtctttttta agaatcaaga aatagttgta gtagggggag gaaatagtgc agtagaagaa gctttatact taaccaatca tgctaacaaa gtcactatag tgcatagaag agatagtttt agagctgaga aaatattaca ggatcgatta tttaaaaatt ctaaaatatc agtaatttgg gatcatgtag tagatgagat tgtaggtagc aataaaccaa aatccgttac tggagttaaa attcaaaatg ttcacaccaa agagattagt ttattgaatt gtagcggtgt atttatagct attgggcatg caccgaatac ngggctattt accggacaaa ttgcaatgga tgacgatnat tatatcatca cgaagtcagg cactactaga actagtgtag aannngtttt tgctgctggt gacgttcnta aaatttatag acaagcagta actgctgcag gaacaggctg tatggcggct cnnagnctga aaatttctcn ataaataant gagtaagana gnatncgctg nnnnnnnnnn cccnaaannn nnnnnnnnnn nnnnaaaaaa annnnnnnnn nnnnnnnnan nnntnnnnnn nnnnnnnncn naannnnnnn nnnnnnannn nnnnnangnn nnnnannnnn nnncaannnn annnnnnnnn nnnnnnnnng nnnnnnnnnn nnnnanc
Expected Protein Sequence
MAHHHHHHMA ALKPILINGM QPGGQLTITT GVENYPGFAE TVQGPWLMEQ MYMQAKNVGT EIVSDYVEKV DLSKRPFKVF TGAGNEYDAE SIIICTGAEA KWLGIASEQK FRGFGVSACA TCDGVFFKNQ EIVVVGGGNS AVEEALYLTN HANKVTIVHR RDSFRAEKIL QDRLFKNSKI SVIWDHVVDE IVGSNKPKSV TGVKIQNVHT KEISLLNCSG VFIAIGHAPN TGLFTGQIAM DDDNYIITKS GTTRTSVEGV FAAGDVQDKI YRQAVTAAGT GCMAALEAEK FLNK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg ctttaaaacc aatattaata aatggtatgc agccaggcgg gcagcttaca attaccaccg gtgttgagaa ctatccagga tttgcggaaa cggtgcaagg accttggctt atggagcaga tgtatatgca ggctaaaaat gtcggtacgg aaattgttag tgattacgta gaaaaggtgg atttatcaaa aagacctttt aaagtattta caggagcagg aaatgaatat gacgcggaga gcataatcat ttgtaccggt gcggaagcaa aatggcttgg tatagcttca gagcagaaat ttcgtggttt tggtgtttcg gcatgtgcta cttgcgatgg tgtctttttt aagaatcaag aaatagttgt agtaggggga ggaaatagtg cagtagaaga agctttatac ttaaccaatc atgctaacaa agtcactata gtgcatagaa gagatagttt tagagctgag aaaatattac aggatcgatt atttaaaaat tctaaaatat cagtaatttg ggatcatgta gtagatgaga ttgtaggtag caataaacca aaatccgtta ctggagttaa aattcaaaat gttcacacca aagagattag tttattgaat tgtagcggtg tatttatagc tattgggcat gcaccgaata cggggctatt taccggacaa attgcaatgg atgacgataa ttatatcatc acgaagtcag gcactactag aactagtgta gaaggtgttt ttgctgctgg tgacgttcag gataaaattt atagacaagc agtaactgct gcaggaacag gctgtatggc ggctcttgag gctgaaaaat ttctcaataa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat