SSGCID: Target Page

RiriA.00159.a

ATP-dependent RNA helicase RhlE

CENTER ID:	RiriA.00159.a
ORGANISM:	Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE:	Rocky Mountain Spotted fever
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiriA.00159.a.B1.GE36180	Full length( RiriA.00159.a )		1	410

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiriA.00159.a.B1.PW36825	Full length( RiriA.00159.a )		1	410

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|392021.5.peg.1186
RefSeq:	YP_001495116.1
UniProt:	A0A0H3AXD1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKNFNLSEEL IIALETMNIT EPTEIQKQSI PVAMAGSDIL ASSQTGSGKT LAYLLPLIDS FIKNKTTALI LVPTRELATQ IHSTLNKVTT SYKINSAVLI GGEPMPKQFM QLKKNPKVII GTPGRIIDHL NRGSLKIDRI GITVLDEMDR MLDMGMKEQL EEINKFLPEK RQVLMFSATM PKHIIAVSQK YLNNPVRITV GATNKAAAEI KQESMHVSDK EKFSALTKQL GHREGSVIIF VKTKRSADQL AKMLKYENHT AEAIHGDLSQ RQRERVILSF RKSNHRIMVA TDVAARGLDI PHTQHVINYD LPMCPEDYLH RIGRTGRAGA TGHALSFISP DDVIRWRAID RLVNKGESTP RSEFRSDKNN RKRSFGKRAG GDGKKFNAFD SSRKRTFDGN SYGNKRKKVS

NT Sequence

ATGAAAAATT TTAATTTATC TGAAGAATTA ATTATTGCTC TCGAGACAAT GAATATAACT GAGCCGACTG AAATACAAAA GCAATCGATT CCGGTTGCAA TGGCGGGATC GGACATACTC GCTTCTAGCC AAACAGGTTC AGGCAAAACT CTTGCTTATT TATTGCCGTT AATTGATTCA TTTATTAAAA ATAAAACTAC TGCTTTAATT CTTGTTCCGA CTAGAGAATT AGCAACGCAA ATACACAGTA CTTTAAATAA AGTAACTACA TCCTATAAAA TTAATAGTGC AGTTTTGATA GGCGGTGAAC CGATGCCTAA ACAATTTATG CAATTAAAAA AGAATCCAAA AGTGATTATC GGTACGCCGG GGCGTATTAT CGATCACTTA AATAGGGGAA GTCTAAAAAT TGATCGTATA GGCATAACCG TACTTGACGA AATGGATAGA ATGCTTGATA TGGGGATGAA AGAACAATTA GAAGAAATCA ATAAATTTTT ACCGGAAAAA AGACAAGTTT TAATGTTTTC TGCTACTATG CCAAAACATA TTATTGCTGT TTCTCAAAAA TATCTAAACA ATCCGGTACG TATTACGGTA GGTGCTACTA ATAAAGCAGC TGCAGAAATA AAACAGGAAT CAATGCATGT TTCTGATAAA GAAAAATTTA GTGCATTAAC TAAACAACTT GGTCATAGGG AAGGATCGGT CATTATTTTT GTGAAGACTA AACGCTCTGC CGATCAATTA GCTAAAATGT TAAAATATGA AAATCATACA GCAGAAGCTA TACACGGTGA TTTAAGTCAG CGTCAACGTG AAAGAGTAAT TTTATCATTT CGTAAGTCAA ACCATAGAAT AATGGTAGCA ACTGATGTAG CGGCTCGTGG GCTTGATATT CCTCATACAC AGCATGTGAT TAATTATGAT TTACCTATGT GTCCTGAAGA TTATTTACAT AGGATAGGTA GAACGGGTAG AGCAGGGGCT ACTGGACATG CGTTGTCTTT TATTTCTCCT GATGATGTTA TTAGATGGCG TGCAATCGAT CGTTTAGTAA ATAAAGGAGA ATCTACGCCA CGTAGTGAGT TTAGAAGTGA TAAAAATAAT CGTAAAAGAT CATTCGGTAA AAGAGCCGGC GGTGACGGTA AAAAGTTTAA TGCTTTTGAT AGTAGCCGTA AAAGAACCTT TGATGGTAAC AGCTACGGTA ATAAAAGAAA GAAAGTATCG

Details for RiriA.00159.a.B1.GE36180

HARVESTED ON:	9/26/2012
SEQUENCED ON:	9/25/2012
EXPECTED MW:	47kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	90

Validated AA Sequence

MNITEPTEIQ KQSIPVAMAG SDILASSQTG SGKTLAYLLP LIDSFIKNKT TALILVPTRE LATQIHSTLN KVTTSYKINS AVLIGGEPMP KQFMQLKKNP KVIIGTPGRI IDHLNRGSLK IDRIGITVLD EMDRMLDMGM KEQLEEINKF LPEKRQVLMF SATMPKHIIA VSQKYLNNPV RITVGATNKA AAEIKQESMH VSDKEKFSAL TKQLGHREGS VIIFVKTKRS ADQLAKMLKY ENHTAEAIHG DLSQRQRERV ILSFRKSNHR IMVATDVAAR GLDIPHTQHV INYDLPMCPE DYLHRIGRTG RAGATGHALS FISPDDVIRW RAIDRLVNKG ESTPRSEFRS DKNNRKRSFG KRAGGDGKKF NAFDSSRK

Validated NT Sequence

cttttacggc tactatcaaa agcattaaac tttttaccgt caccgccggc tcttttaccg aatgatcttt tacgattatt tttatcactt ctaaactcac tacgtggcgt agattctcct ttatttacta aacgatcgat tgcacgccat ctaataacat catcaggaga aataaaagac aacgcatgtc cagtagcccc tgctctaccc gttctaccta tcctatgtaa ataatcttca ggacacatag gtaaatcata attaatcaca tgctgtgtat gaggaatatc aagcccacga gccgctacat cagttgctac cattattcta tggtttgact tacgaaatga taaaattact ctttcacgtt gacgctgact taaatcaccg tgtatagctt ctgctgtatg attttcatat tttaacattt tagctaattg atcggcagag cgtttagtct tcacaaaaat aatgaccgat ccttccctat gaccaagttg tttagttaat gcactaaatt tttctttatc agaaacatgc attgattcct gttttatttc tgcagctgct ttattagtag cacctaccgt aatacgtacc ggattgttta gatatttttg agaaacagca ataatatgtt ttggcatagt agcagaaaac attaaaactt gtcttttttc cggtaaaaat ttattgattt cttctaattg ttctttcatc cccatatcaa gcattctatc catttcgtca agtacggtta tgcctatacg atcaattttt agacttcccc tatttaagtg atcgataata cgccccggcg taccgataat cacttttgga ttctttttta attgcataaa ttgtttaggc atcggttcac cgcctatcaa aactgcacta ttaattttat aggatgtagt tactttattt aaagtactgt gtatttgcgt tgctaattct ctagtcggaa caagaattaa agcagtagtt ttatttttaa taaatgaatc aattaacggc aataaataag caagagtttt gcctgaacct gtttggctag aagcgagtat gtccgatccc gccattgcaa ccggaatcga ttgcttttgt atttcagtcg gctcagttat attcattgtc tcgagagcaa taattaattc ttcagataaa

Expected Protein Sequence

MAHHHHHHMK NFNLSEELII ALETMNITEP TEIQKQSIPV AMAGSDILAS SQTGSGKTLA YLLPLIDSFI KNKTTALILV PTRELATQIH STLNKVTTSY KINSAVLIGG EPMPKQFMQL KKNPKVIIGT PGRIIDHLNR GSLKIDRIGI TVLDEMDRML DMGMKEQLEE INKFLPEKRQ VLMFSATMPK HIIAVSQKYL NNPVRITVGA TNKAAAEIKQ ESMHVSDKEK FSALTKQLGH REGSVIIFVK TKRSADQLAK MLKYENHTAE AIHGDLSQRQ RERVILSFRK SNHRIMVATD VAARGLDIPH TQHVINYDLP MCPEDYLHRI GRTGRAGATG HALSFISPDD VIRWRAIDRL VNKGESTPRS EFRSDKNNRK RSFGKRAGGD GKKFNAFDSS RKRTFDGNSY GNKRKKVS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa attttaattt atctgaagaa ttaattattg ctctcgagac aatgaatata actgagccga ctgaaataca aaagcaatcg attccggttg caatggcggg atcggacata ctcgcttcta gccaaacagg ttcaggcaaa actcttgctt atttattgcc gttaattgat tcatttatta aaaataaaac tactgcttta attcttgttc cgactagaga attagcaacg caaatacaca gtactttaaa taaagtaact acatcctata aaattaatag tgcagttttg ataggcggtg aaccgatgcc taaacaattt atgcaattaa aaaagaatcc aaaagtgatt atcggtacgc cggggcgtat tatcgatcac ttaaataggg gaagtctaaa aattgatcgt ataggcataa ccgtacttga cgaaatggat agaatgcttg atatggggat gaaagaacaa ttagaagaaa tcaataaatt tttaccggaa aaaagacaag ttttaatgtt ttctgctact atgccaaaac atattattgc tgtttctcaa aaatatctaa acaatccggt acgtattacg gtaggtgcta ctaataaagc agctgcagaa ataaaacagg aatcaatgca tgtttctgat aaagaaaaat ttagtgcatt aactaaacaa cttggtcata gggaaggatc ggtcattatt tttgtgaaga ctaaacgctc tgccgatcaa ttagctaaaa tgttaaaata tgaaaatcat acagcagaag ctatacacgg tgatttaagt cagcgtcaac gtgaaagagt aattttatca tttcgtaagt caaaccatag aataatggta gcaactgatg tagcggctcg tgggcttgat attcctcata cacagcatgt gattaattat gatttaccta tgtgtcctga agattattta cataggatag gtagaacggg tagagcaggg gctactggac atgcgttgtc ttttatttct cctgatgatg ttattagatg gcgtgcaatc gatcgtttag taaataaagg agaatctacg ccacgtagtg agtttagaag tgataaaaat aatcgtaaaa gatcattcgg taaaagagcc ggcggtgacg gtaaaaagtt taatgctttt gatagtagcc gtaaaagaac ctttgatggt aacagctacg gtaataaaag aaagaaagta tcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for RiriA.00159.a.B1.PW36825

PURIFICATION DATe:	12/6/2012
CONCENTRATION:	18mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	90

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA ATTTTAATTT ATCTGAAGAA TTAATTATTG CTCTCGAGAC AATGAATATA ACTGAGCCGA CTGAAATACA AAAGCAATCG ATTCCGGTTG CAATGGCGGG ATCGGACATA CTCGCTTCTA GCCAAACAGG TTCAGGCAAA ACTCTTGCTT ATTTATTGCC GTTAATTGAT TCATTTATTA AAAATAAAAC TACTGCTTTA ATTCTTGTTC CGACTAGAGA ATTAGCAACG CAAATACACA GTACTTTAAA TAAAGTAACT ACATCCTATA AAATTAATAG TGCAGTTTTG ATAGGCGGTG AACCGATGCC TAAACAATTT ATGCAATTAA AAAAGAATCC AAAAGTGATT ATCGGTACGC CGGGGCGTAT TATCGATCAC TTAAATAGGG GAAGTCTAAA AATTGATCGT ATAGGCATAA CCGTACTTGA CGAAATGGAT AGAATGCTTG ATATGGGGAT GAAAGAACAA TTAGAAGAAA TCAATAAATT TTTACCGGAA AAAAGACAAG TTTTAATGTT TTCTGCTACT ATGCCAAAAC ATATTATTGC TGTTTCTCAA AAATATCTAA ACAATCCGGT ACGTATTACG GTAGGTGCTA CTAATAAAGC AGCTGCAGAA ATAAAACAGG AATCAATGCA TGTTTCTGAT AAAGAAAAAT TTAGTGCATT AACTAAACAA CTTGGTCATA GGGAAGGATC GGTCATTATT TTTGTGAAGA CTAAACGCTC TGCCGATCAA TTAGCTAAAA TGTTAAAATA TGAAAATCAT ACAGCAGAAG CTATACACGG TGATTTAAGT CAGCGTCAAC GTGAAAGAGT AATTTTATCA TTTCGTAAGT CAAACCATAG AATAATGGTA GCAACTGATG TAGCGGCTCG TGGGCTTGAT ATTCCTCATA CACAGCATGT GATTAATTAT GATTTACCTA TGTGTCCTGA AGATTATTTA CATAGGATAG GTAGAACGGG TAGAGCAGGG GCTACTGGAC ATGCGTTGTC TTTTATTTCT CCTGATGATG TTATTAGATG GCGTGCAATC GATCGTTTAG TAAATAAAGG AGAATCTACG CCACGTAGTG AGTTTAGAAG TGATAAAAAT AATCGTAAAA GATCATTCGG TAAAAGAGCC GGCGGTGACG GTAAAAAGTT TAATGCTTTT GATAGTAGCC GTAAAAGAAC CTTTGATGGT AACAGCTACG GTAATAAAAG AAAGAAAGTA TCGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT