RiriA.00148.a

Tyrosine recombinase

CENTER ID: RiriA.00148.a
ORGANISM: Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE: Rocky Mountain Spotted fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.00148.a.B1.GE35618 Full length( RiriA.00148.a ) 1 306
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.00148.a.B1.PW36252 Full length( RiriA.00148.a ) 1 306

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|392021.5.peg.587
RefSeq: YP_001494604.1
UniProt: A0A0H3AWP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEFIAQFLEM LLAERALSKN SILSYKRDLF DFQNYLAKHK LSELNITTEN IRDWIEYLAS NDLQARSINR KISTIKSYYE FLISENHTAF NPVLNVDLPK YQNKLPEILS IAQIKSLLEH CSQDNSPEGI RLNAMIHLLY ASGLRVSELV SLKLADILTN KTSKGEVRKI FSVLGKGNKE RVIVINEQAV ISIAKYLAIR DVFVNKAKPR NLIYLFPSSA LAGYMTRQNF AILLKSAALY AGLNPEYISP HILRHSFASH LLEGGADLRV IQELLGHADI STTQIYTHLQ TNHLKKALLH HPLNKN
NT Sequence
ATGGAATTTA TTGCGCAATT CCTAGAAATG CTTTTAGCCG AGAGAGCATT GTCAAAAAAT TCTATACTGA GCTATAAGCG TGACTTATTC GATTTTCAAA ATTATCTTGC TAAACACAAA TTATCCGAAT TAAATATTAC TACTGAGAAT ATTAGAGACT GGATTGAGTA TCTAGCAAGT AATGATTTGC AAGCACGCTC AATCAACAGA AAAATCTCAA CTATAAAAAG TTATTATGAA TTTTTAATTA GCGAAAACCA TACCGCGTTT AATCCGGTTC TTAACGTAGA TTTGCCTAAA TATCAAAATA AACTTCCCGA AATATTATCT ATAGCTCAAA TTAAATCGTT ACTTGAACAT TGCTCTCAAG ATAATTCTCC TGAAGGTATC AGGCTTAATG CTATGATTCA TTTGCTTTAT GCTAGCGGTC TTAGAGTCTC GGAGCTTGTG AGTCTTAAGC TTGCTGATAT TCTGACTAAT AAAACGTCTA AAGGAGAAGT AAGAAAAATA TTTTCGGTAC TTGGTAAAGG TAATAAGGAA AGAGTCATAG TAATAAATGA ACAGGCAGTT ATCAGTATTG CTAAATATCT TGCAATTAGA GATGTTTTTG TGAATAAAGC TAAACCAAGA AATCTAATTT ATTTATTTCC TTCATCAGCT TTAGCGGGTT ATATGACTAG GCAAAATTTT GCTATTCTGT TAAAATCTGC TGCCCTTTAT GCAGGACTTA ACCCTGAGTA TATTTCTCCG CATATATTAC GTCACAGTTT TGCAAGTCAT TTGCTTGAGG GCGGAGCAGA CTTAAGAGTA ATTCAAGAGC TGCTTGGTCA CGCTGATATA TCTACTACCC AAATATATAC TCATCTTCAA ACTAATCATC TCAAAAAAGC ATTGTTGCAT CATCCTCTCA ATAAAAAT
Details for RiriA.00148.a.B1.GE35618
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 87
Validated AA Sequence
MAHHHHHHME FIAQFLEMLL AERALSKNSI LSYKRDLFDF QNYLAKHKLS ELNITTENIR DWIEYLASND LQARSINRKI STIKSYYEFL ISENHTAFNP VLNVDLPKYQ NKLPEILSIA QIKSLLEHCS QDNSPEGIRL NAMIHLLYAS GLRVSELVSL KLADILTNKT SKGEVRKIFS VLGKGNKERV IVINEQAVIS IAKYLAIRDV FVNKAKPRNL IYLFPSSALA GYMTRQNFAI LLKSAALYAD LTLSIFLRIY YVTVLQVICL XAXQT
Validated NT Sequence
ttgtttactt taagaaggag atataccatg gctcaccacc accaccacca tatggaattt attgcgcaat tcctagaaat gcttttagcc gagagagcat tgtcaaaaaa ttctatactg agctataagc gtgacttatt cgattttcaa aattatcttg ctaaacacaa attatccgaa ttaaatatta ctactgagaa tattagagac tggattgagt atctagcaag taatgatttg caagcacgct caatcaacag aaaaatctca actataaaaa gttattatga atttttaatt agcgaaaacc ataccgcgtt taatccggtt cttaacgtag atttgcctaa atatcaaaat aaacttcccg aaatattatc tatagctcaa attaaatcgt tacttgaaca ttgctctcaa gataattctc ctgaaggtat caggcttaat gctatgattc atttgcttta tgctagcggt cttagagtct cggagcttgt gagtcttaag cttgctgata ttctgactaa taaaacgtct aaaggagaag taagaaaaat attttcggta cttggtaaag gtaataagga aagagtcata gtaataaatg aacaggcagt tatcagtatt gctaaatatc ttgcaattag agatgttttt gtgaataaag ctaaaccaag aaatctaatt tatttatttc cttcatcagc tttagcgggt tatatgacta ggcaaaattt tgctattctg ttaaaatctg ctgcccttta tgcngactta accctgagta tatttctccg catatattac gtcacagttt tgcaagtcat ttgcttgang gcggancaga cttaagagta attcaanagc tgcttgntca cgctgatnta tctactaccc aaatanatac tcatcttcaa nctaatcntc tcaaaaagca ttgnnncatc atcnnncann aaaantaann ganannannn nannnnnctg ntannnnnnc cnna
Expected Protein Sequence
MAHHHHHHME FIAQFLEMLL AERALSKNSI LSYKRDLFDF QNYLAKHKLS ELNITTENIR DWIEYLASND LQARSINRKI STIKSYYEFL ISENHTAFNP VLNVDLPKYQ NKLPEILSIA QIKSLLEHCS QDNSPEGIRL NAMIHLLYAS GLRVSELVSL KLADILTNKT SKGEVRKIFS VLGKGNKERV IVINEQAVIS IAKYLAIRDV FVNKAKPRNL IYLFPSSALA GYMTRQNFAI LLKSAALYAG LNPEYISPHI LRHSFASHLL EGGADLRVIQ ELLGHADIST TQIYTHLQTN HLKKALLHHP LNKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat ttattgcgca attcctagaa atgcttttag ccgagagagc attgtcaaaa aattctatac tgagctataa gcgtgactta ttcgattttc aaaattatct tgctaaacac aaattatccg aattaaatat tactactgag aatattagag actggattga gtatctagca agtaatgatt tgcaagcacg ctcaatcaac agaaaaatct caactataaa aagttattat gaatttttaa ttagcgaaaa ccataccgcg tttaatccgg ttcttaacgt agatttgcct aaatatcaaa ataaacttcc cgaaatatta tctatagctc aaattaaatc gttacttgaa cattgctctc aagataattc tcctgaaggt atcaggctta atgctatgat tcatttgctt tatgctagcg gtcttagagt ctcggagctt gtgagtctta agcttgctga tattctgact aataaaacgt ctaaaggaga agtaagaaaa atattttcgg tacttggtaa aggtaataag gaaagagtca tagtaataaa tgaacaggca gttatcagta ttgctaaata tcttgcaatt agagatgttt ttgtgaataa agctaaacca agaaatctaa tttatttatt tccttcatca gctttagcgg gttatatgac taggcaaaat tttgctattc tgttaaaatc tgctgccctt tatgcaggac ttaaccctga gtatatttct ccgcatatat tacgtcacag ttttgcaagt catttgcttg agggcggagc agacttaaga gtaattcaag agctgcttgg tcacgctgat atatctacta cccaaatata tactcatctt caaactaatc atctcaaaaa agcattgttg catcatcctc tcaataaaaa ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for RiriA.00148.a.B1.PW36252
PURIFICATION DATe: 7/30/2012
CONCENTRATION: 27.4mg/ml
OBSERVED MW: 34kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 92
PERCENT COVERAGE: 87
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME FIAQFLEMLL AERALSKNSI LSYKRDLFDF QNYLAKHKLS ELNITTENIR DWIEYLASND LQARSINRKI STIKSYYEFL ISENHTAFNP VLNVDLPKYQ NKLPEILSIA QIKSLLEHCS QDNSPEGIRL NAMIHLLYAS GLRVSELVSL KLADILTNKT SKGEVRKIFS VLGKGNKERV IVINEQAVIS IAKYLAIRDV FVNKAKPRNL IYLFPSSALA GYMTRQNFAI LLKSAALYAD LTLSIFLRIY YVTVLQVICL XAXQT
Validated NT Sequence
ttgtttactt taagaaggag atataccatg gctcaccacc accaccacca tatggaattt attgcgcaat tcctagaaat gcttttagcc gagagagcat tgtcaaaaaa ttctatactg agctataagc gtgacttatt cgattttcaa aattatcttg ctaaacacaa attatccgaa ttaaatatta ctactgagaa tattagagac tggattgagt atctagcaag taatgatttg caagcacgct caatcaacag aaaaatctca actataaaaa gttattatga atttttaatt agcgaaaacc ataccgcgtt taatccggtt cttaacgtag atttgcctaa atatcaaaat aaacttcccg aaatattatc tatagctcaa attaaatcgt tacttgaaca ttgctctcaa gataattctc ctgaaggtat caggcttaat gctatgattc atttgcttta tgctagcggt cttagagtct cggagcttgt gagtcttaag cttgctgata ttctgactaa taaaacgtct aaaggagaag taagaaaaat attttcggta cttggtaaag gtaataagga aagagtcata gtaataaatg aacaggcagt tatcagtatt gctaaatatc ttgcaattag agatgttttt gtgaataaag ctaaaccaag aaatctaatt tatttatttc cttcatcagc tttagcgggt tatatgacta ggcaaaattt tgctattctg ttaaaatctg ctgcccttta tgcngactta accctgagta tatttctccg catatattac gtcacagttt tgcaagtcat ttgcttgang gcggancaga cttaagagta attcaanagc tgcttgntca cgctgatnta tctactaccc aaatanatac tcatcttcaa nctaatcntc tcaaaaagca ttgnnncatc atcnnncann aaaantaann ganannannn nannnnnctg ntannnnnnc cnna
Expressed Protein Sequence
MAHHHHHHME FIAQFLEMLL AERALSKNSI LSYKRDLFDF QNYLAKHKLS ELNITTENIR DWIEYLASND LQARSINRKI STIKSYYEFL ISENHTAFNP VLNVDLPKYQ NKLPEILSIA QIKSLLEHCS QDNSPEGIRL NAMIHLLYAS GLRVSELVSL KLADILTNKT SKGEVRKIFS VLGKGNKERV IVINEQAVIS IAKYLAIRDV FVNKAKPRNL IYLFPSSALA GYMTRQNFAI LLKSAALYAG LNPEYISPHI LRHSFASHLL EGGADLRVIQ ELLGHADIST TQIYTHLQTN HLKKALLHHP LNKN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAT TTATTGCGCA ATTCCTAGAA ATGCTTTTAG CCGAGAGAGC ATTGTCAAAA AATTCTATAC TGAGCTATAA GCGTGACTTA TTCGATTTTC AAAATTATCT TGCTAAACAC AAATTATCCG AATTAAATAT TACTACTGAG AATATTAGAG ACTGGATTGA GTATCTAGCA AGTAATGATT TGCAAGCACG CTCAATCAAC AGAAAAATCT CAACTATAAA AAGTTATTAT GAATTTTTAA TTAGCGAAAA CCATACCGCG TTTAATCCGG TTCTTAACGT AGATTTGCCT AAATATCAAA ATAAACTTCC CGAAATATTA TCTATAGCTC AAATTAAATC GTTACTTGAA CATTGCTCTC AAGATAATTC TCCTGAAGGT ATCAGGCTTA ATGCTATGAT TCATTTGCTT TATGCTAGCG GTCTTAGAGT CTCGGAGCTT GTGAGTCTTA AGCTTGCTGA TATTCTGACT AATAAAACGT CTAAAGGAGA AGTAAGAAAA ATATTTTCGG TACTTGGTAA AGGTAATAAG GAAAGAGTCA TAGTAATAAA TGAACAGGCA GTTATCAGTA TTGCTAAATA TCTTGCAATT AGAGATGTTT TTGTGAATAA AGCTAAACCA AGAAATCTAA TTTATTTATT TCCTTCATCA GCTTTAGCGG GTTATATGAC TAGGCAAAAT TTTGCTATTC TGTTAAAATC TGCTGCCCTT TATGCAGGAC TTAACCCTGA GTATATTTCT CCGCATATAT TACGTCACAG TTTTGCAAGT CATTTGCTTG AGGGCGGAGC AGACTTAAGA GTAATTCAAG AGCTGCTTGG TCACGCTGAT ATATCTACTA CCCAAATATA TACTCATCTT CAAACTAATC ATCTCAAAAA AGCATTGTTG CATCATCCTC TCAATAAAAA TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT