RiriA.00133.a

Cysteinyl-tRNA synthetase

CENTER ID: RiriA.00133.a
ORGANISM: Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE: Rocky Mountain Spotted fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.00133.a.B1.GE36174 Full length( RiriA.00133.a ) 1 459
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.00133.a.B1.PS01691 Full length( RiriA.00133.a ) 1 459

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|392021.5.peg.144
RefSeq: YP_001494229.1
UniProt: A8GQP6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQIQFRLYNT LSRTKEVFNP QDQDNVKMYV CGPTVYYNPH IGNSRSGVVY DLLYRIVIKI FGEKAVKYVR NITDVDDKII DRAALLGVTI DELTDKVTKE FHKNMAYLGC MLPSIEPKAT KHIDVMIAII ERLIAKDHAY IADNHVYFDV LSAPNYTELS NRNLEEMFEG VHVENSKTKK NPQDFVLWKP AKQNESANMN FESPWGLGRP GWHIECSAMS YKYLGENFDI HGGGADLIFP HHTNEIAQSR CAFPSSTYAK YWVHNGFLTV NGEKMSKSLG NFITVRDLMD KQIQGEVVRL FLLSSHYRRP LDYNDKAIED AKKTLDYWYR AIENINVQKI DLPHDFMQSL LDDMNTPLAV KIINDYAKGV FISKTEEERQ LNASAIITCA NFIGLMNKSP HEWFNSGVDE LYINELVNKR LEAKKHKNWL LADQIRNQLL EEKIILEDQP DGTTIWRKE
NT Sequence
ATGCAAATTC AATTTCGCTT ATATAACACT CTTAGTCGTA CTAAAGAGGT TTTTAATCCT CAAGATCAAG ATAACGTAAA AATGTATGTA TGTGGACCGA CTGTTTATTA TAATCCTCAT ATAGGCAATA GTAGGTCGGG AGTAGTATAT GATTTACTTT ATCGTATAGT TATAAAAATA TTCGGTGAGA AGGCAGTAAA ATATGTACGT AACATTACGG ATGTTGACGA TAAAATTATC GATAGAGCTG CACTACTTGG TGTTACTATT GATGAATTGA CTGATAAAGT TACGAAAGAG TTTCATAAAA ATATGGCATA TTTAGGTTGT ATGCTACCTA GTATTGAGCC GAAAGCTACA AAGCATATTG ATGTAATGAT CGCAATAATA GAACGCTTAA TAGCAAAAGA TCACGCATAT ATTGCTGATA ATCATGTTTA TTTTGATGTT TTATCAGCGC CAAATTATAC GGAATTATCT AACCGAAATT TAGAAGAAAT GTTTGAGGGA GTGCATGTAG AAAATAGTAA AACCAAAAAA AATCCACAAG ATTTTGTATT ATGGAAACCG GCAAAGCAAA ATGAATCGGC AAATATGAAT TTTGAAAGCC CCTGGGGACT TGGTCGCCCT GGATGGCATA TTGAATGTTC AGCCATGAGC TACAAATATT TAGGTGAGAA TTTTGATATT CACGGCGGCG GAGCAGATTT AATCTTTCCG CATCATACCA ATGAGATTGC TCAAAGTAGA TGTGCTTTTC CAAGTTCTAC CTATGCTAAA TATTGGGTAC ATAATGGGTT TCTAACGGTT AACGGTGAGA AAATGAGTAA ATCTCTTGGT AATTTTATTA CTGTAAGGGA TTTAATGGAT AAACAAATTC AAGGAGAAGT AGTAAGGTTA TTTTTATTAA GTAGTCACTA TAGGCGTCCT CTTGATTATA ATGATAAAGC TATAGAAGAT GCTAAGAAAA CTTTAGATTA TTGGTATAGA GCTATAGAAA ATATTAATGT GCAGAAAATA GATTTACCTC ATGATTTCAT GCAAAGCTTA CTTGATGATA TGAATACGCC GCTTGCCGTT AAAATCATTA ATGACTACGC TAAAGGTGTT TTTATTTCTA AAACTGAAGA AGAAAGGCAG CTTAACGCTT CTGCTATTAT TACTTGTGCT AATTTTATTG GTTTAATGAA TAAGAGTCCA CACGAGTGGT TTAATAGCGG TGTGGATGAA CTCTATATAA ATGAGCTTGT AAATAAGCGT TTGGAAGCAA AAAAACACAA AAATTGGTTA TTAGCCGATC AAATTCGTAA CCAGTTATTA GAGGAAAAAA TAATTCTAGA AGATCAACCT GATGGTACTA CTATTTGGCG AAAAGAA
Details for RiriA.00133.a.B1.GE36174
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Validated AA Sequence
MYVCGPTVYY NPHIGNSRSG VVYDLLYRIV IKIFGEKAVK YVRNITDVDD KIIDRAALLG VTIDELTDKV TKEFHKNMAY LGCMLPSIEP KATKHIDVMI AIIERLIAKD HAYIADNHVY FDVLSAPNYT ELSNRNLEEM FEGVHVENSK TKKNPQDFVL WKPAKQNESA NMNFESPWGL GRPGWHIECS AMSYKYLGEN FDIHGGGADL IFPHHTNEIA QSRCAFPSST YAKYWVHNGF LTVNGEKMSK SLGNFITVRD LMDKQIQGEV VRLFLLSSHY RRPLDYNDKA IEDAKKTLDY WYRAIENINV QKIDLPHDFM QSLLDDMNTP LAVKIINDYA KGVFISKTEE ERQLNASAII TCANFIGLMN KSPHEWFNSG VDELYINELV NKRLEAKKHK NWLLADQIRN QLLEEKIIL
Validated NT Sequence
tctagaatta ttttttcctc taataactgg ttacgaattt gatcggctaa taaccaattt ttgtgttttt ttgcttccaa acgcttattt acaagctcat ttatatagag ttcatccaca ccgctattaa accactcgtg tggactctta ttcattaaac caataaaatt agcacaagta ataatagcag aagcgttaag ctgcctttct tcttcagttt tagaaataaa aacaccttta gcgtagtcat taatgatttt aacggcaagc ggcgtattca tatcatcaag taagctttgc atgaaatcat gaggtaaatc tattttctgc acattaatat tttctatagc tctataccaa taatctaaag ttttcttagc atcttctata gctttatcat tataatcaag aggacgccta tagtgactac ttaataaaaa taaccttact acttctcctt gaatttgttt atccattaaa tcccttacag taataaaatt accaagagat ttactcattt tctcaccgtt aaccgttaga aacccattat gtacccaata tttagcatag gtagaacttg gaaaagcaca tctactttga gcaatctcat tggtatgatg cggaaagatt aaatctgctc cgccgccgtg aatatcaaaa ttctcaccta aatatttgta gctcatggct gaacattcaa tatgccatcc agggcgacca agtccccagg ggctttcaaa attcatattt gccgattcat tttgctttgc cggtttccat aatacaaaat cttgtggatt ttttttggtt ttactatttt ctacatgcac tccctcaaac atttcttcta aatttcggtt agataattcc gtataatttg gcgctgataa aacatcaaaa taaacatgat tatcagcaat atatgcgtga tcttttgcta ttaagcgttc tattattgcg atcattacat caatatgctt tgtagctttc ggctcaatac taggtagcat acaacctaaa tatgccatat ttttatgaaa ctctttcgta actttatcag tcaattcatc aatagtaaca ccaagtagtg cagctctatc gataatttta tcgtcaacat ccgtaatgtt acgtacatat tttactgcct tctcaccgaa tatttttata actatacgat aaagtaaatc atatactact cccgacctac tattgcctat atgaggatta taataaacag tcggtccaca tacatacatt tttacgttat cttgatcttg aggattaaaa acctctttag tacgactaag agtgttatat aagcga
Expected Protein Sequence
MAHHHHHHMQ IQFRLYNTLS RTKEVFNPQD QDNVKMYVCG PTVYYNPHIG NSRSGVVYDL LYRIVIKIFG EKAVKYVRNI TDVDDKIIDR AALLGVTIDE LTDKVTKEFH KNMAYLGCML PSIEPKATKH IDVMIAIIER LIAKDHAYIA DNHVYFDVLS APNYTELSNR NLEEMFEGVH VENSKTKKNP QDFVLWKPAK QNESANMNFE SPWGLGRPGW HIECSAMSYK YLGENFDIHG GGADLIFPHH TNEIAQSRCA FPSSTYAKYW VHNGFLTVNG EKMSKSLGNF ITVRDLMDKQ IQGEVVRLFL LSSHYRRPLD YNDKAIEDAK KTLDYWYRAI ENINVQKIDL PHDFMQSLLD DMNTPLAVKI INDYAKGVFI SKTEEERQLN ASAIITCANF IGLMNKSPHE WFNSGVDELY INELVNKRLE AKKHKNWLLA DQIRNQLLEE KIILEDQPDG TTIWRKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa ttcaatttcg cttatataac actcttagtc gtactaaaga ggtttttaat cctcaagatc aagataacgt aaaaatgtat gtatgtggac cgactgttta ttataatcct catataggca atagtaggtc gggagtagta tatgatttac tttatcgtat agttataaaa atattcggtg agaaggcagt aaaatatgta cgtaacatta cggatgttga cgataaaatt atcgatagag ctgcactact tggtgttact attgatgaat tgactgataa agttacgaaa gagtttcata aaaatatggc atatttaggt tgtatgctac ctagtattga gccgaaagct acaaagcata ttgatgtaat gatcgcaata atagaacgct taatagcaaa agatcacgca tatattgctg ataatcatgt ttattttgat gttttatcag cgccaaatta tacggaatta tctaaccgaa atttagaaga aatgtttgag ggagtgcatg tagaaaatag taaaaccaaa aaaaatccac aagattttgt attatggaaa ccggcaaagc aaaatgaatc ggcaaatatg aattttgaaa gcccctgggg acttggtcgc cctggatggc atattgaatg ttcagccatg agctacaaat atttaggtga gaattttgat attcacggcg gcggagcaga tttaatcttt ccgcatcata ccaatgagat tgctcaaagt agatgtgctt ttccaagttc tacctatgct aaatattggg tacataatgg gtttctaacg gttaacggtg agaaaatgag taaatctctt ggtaatttta ttactgtaag ggatttaatg gataaacaaa ttcaaggaga agtagtaagg ttatttttat taagtagtca ctataggcgt cctcttgatt ataatgataa agctatagaa gatgctaaga aaactttaga ttattggtat agagctatag aaaatattaa tgtgcagaaa atagatttac ctcatgattt catgcaaagc ttacttgatg atatgaatac gccgcttgcc gttaaaatca ttaatgacta cgctaaaggt gtttttattt ctaaaactga agaagaaagg cagcttaacg cttctgctat tattacttgt gctaatttta ttggtttaat gaataagagt ccacacgagt ggtttaatag cggtgtggat gaactctata taaatgagct tgtaaataag cgtttggaag caaaaaaaca caaaaattgg ttattagccg atcaaattcg taaccagtta ttagaggaaa aaataattct agaagatcaa cctgatggta ctactatttg gcgaaaagaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for RiriA.00133.a.B1.PS01691
PURIFICATION DATe: 11/29/2012
CONCENTRATION: 72mg/ml
OBSERVED MW: 52kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MYVCGPTVYY NPHIGNSRSG VVYDLLYRIV IKIFGEKAVK YVRNITDVDD KIIDRAALLG VTIDELTDKV TKEFHKNMAY LGCMLPSIEP KATKHIDVMI AIIERLIAKD HAYIADNHVY FDVLSAPNYT ELSNRNLEEM FEGVHVENSK TKKNPQDFVL WKPAKQNESA NMNFESPWGL GRPGWHIECS AMSYKYLGEN FDIHGGGADL IFPHHTNEIA QSRCAFPSST YAKYWVHNGF LTVNGEKMSK SLGNFITVRD LMDKQIQGEV VRLFLLSSHY RRPLDYNDKA IEDAKKTLDY WYRAIENINV QKIDLPHDFM QSLLDDMNTP LAVKIINDYA KGVFISKTEE ERQLNASAII TCANFIGLMN KSPHEWFNSG VDELYINELV NKRLEAKKHK NWLLADQIRN QLLEEKIIL
Validated NT Sequence
tctagaatta ttttttcctc taataactgg ttacgaattt gatcggctaa taaccaattt ttgtgttttt ttgcttccaa acgcttattt acaagctcat ttatatagag ttcatccaca ccgctattaa accactcgtg tggactctta ttcattaaac caataaaatt agcacaagta ataatagcag aagcgttaag ctgcctttct tcttcagttt tagaaataaa aacaccttta gcgtagtcat taatgatttt aacggcaagc ggcgtattca tatcatcaag taagctttgc atgaaatcat gaggtaaatc tattttctgc acattaatat tttctatagc tctataccaa taatctaaag ttttcttagc atcttctata gctttatcat tataatcaag aggacgccta tagtgactac ttaataaaaa taaccttact acttctcctt gaatttgttt atccattaaa tcccttacag taataaaatt accaagagat ttactcattt tctcaccgtt aaccgttaga aacccattat gtacccaata tttagcatag gtagaacttg gaaaagcaca tctactttga gcaatctcat tggtatgatg cggaaagatt aaatctgctc cgccgccgtg aatatcaaaa ttctcaccta aatatttgta gctcatggct gaacattcaa tatgccatcc agggcgacca agtccccagg ggctttcaaa attcatattt gccgattcat tttgctttgc cggtttccat aatacaaaat cttgtggatt ttttttggtt ttactatttt ctacatgcac tccctcaaac atttcttcta aatttcggtt agataattcc gtataatttg gcgctgataa aacatcaaaa taaacatgat tatcagcaat atatgcgtga tcttttgcta ttaagcgttc tattattgcg atcattacat caatatgctt tgtagctttc ggctcaatac taggtagcat acaacctaaa tatgccatat ttttatgaaa ctctttcgta actttatcag tcaattcatc aatagtaaca ccaagtagtg cagctctatc gataatttta tcgtcaacat ccgtaatgtt acgtacatat tttactgcct tctcaccgaa tatttttata actatacgat aaagtaaatc atatactact cccgacctac tattgcctat atgaggatta taataaacag tcggtccaca tacatacatt tttacgttat cttgatcttg aggattaaaa acctctttag tacgactaag agtgttatat aagcga
Expressed Protein Sequence
MAHHHHHHMQ IQFRLYNTLS RTKEVFNPQD QDNVKMYVCG PTVYYNPHIG NSRSGVVYDL LYRIVIKIFG EKAVKYVRNI TDVDDKIIDR AALLGVTIDE LTDKVTKEFH KNMAYLGCML PSIEPKATKH IDVMIAIIER LIAKDHAYIA DNHVYFDVLS APNYTELSNR NLEEMFEGVH VENSKTKKNP QDFVLWKPAK QNESANMNFE SPWGLGRPGW HIECSAMSYK YLGENFDIHG GGADLIFPHH TNEIAQSRCA FPSSTYAKYW VHNGFLTVNG EKMSKSLGNF ITVRDLMDKQ IQGEVVRLFL LSSHYRRPLD YNDKAIEDAK KTLDYWYRAI ENINVQKIDL PHDFMQSLLD DMNTPLAVKI INDYAKGVFI SKTEEERQLN ASAIITCANF IGLMNKSPHE WFNSGVDELY INELVNKRLE AKKHKNWLLA DQIRNQLLEE KIILEDQPDG TTIWRKE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA TTCAATTTCG CTTATATAAC ACTCTTAGTC GTACTAAAGA GGTTTTTAAT CCTCAAGATC AAGATAACGT AAAAATGTAT GTATGTGGAC CGACTGTTTA TTATAATCCT CATATAGGCA ATAGTAGGTC GGGAGTAGTA TATGATTTAC TTTATCGTAT AGTTATAAAA ATATTCGGTG AGAAGGCAGT AAAATATGTA CGTAACATTA CGGATGTTGA CGATAAAATT ATCGATAGAG CTGCACTACT TGGTGTTACT ATTGATGAAT TGACTGATAA AGTTACGAAA GAGTTTCATA AAAATATGGC ATATTTAGGT TGTATGCTAC CTAGTATTGA GCCGAAAGCT ACAAAGCATA TTGATGTAAT GATCGCAATA ATAGAACGCT TAATAGCAAA AGATCACGCA TATATTGCTG ATAATCATGT TTATTTTGAT GTTTTATCAG CGCCAAATTA TACGGAATTA TCTAACCGAA ATTTAGAAGA AATGTTTGAG GGAGTGCATG TAGAAAATAG TAAAACCAAA AAAAATCCAC AAGATTTTGT ATTATGGAAA CCGGCAAAGC AAAATGAATC GGCAAATATG AATTTTGAAA GCCCCTGGGG ACTTGGTCGC CCTGGATGGC ATATTGAATG TTCAGCCATG AGCTACAAAT ATTTAGGTGA GAATTTTGAT ATTCACGGCG GCGGAGCAGA TTTAATCTTT CCGCATCATA CCAATGAGAT TGCTCAAAGT AGATGTGCTT TTCCAAGTTC TACCTATGCT AAATATTGGG TACATAATGG GTTTCTAACG GTTAACGGTG AGAAAATGAG TAAATCTCTT GGTAATTTTA TTACTGTAAG GGATTTAATG GATAAACAAA TTCAAGGAGA AGTAGTAAGG TTATTTTTAT TAAGTAGTCA CTATAGGCGT CCTCTTGATT ATAATGATAA AGCTATAGAA GATGCTAAGA AAACTTTAGA TTATTGGTAT AGAGCTATAG AAAATATTAA TGTGCAGAAA ATAGATTTAC CTCATGATTT CATGCAAAGC TTACTTGATG ATATGAATAC GCCGCTTGCC GTTAAAATCA TTAATGACTA CGCTAAAGGT GTTTTTATTT CTAAAACTGA AGAAGAAAGG CAGCTTAACG CTTCTGCTAT TATTACTTGT GCTAATTTTA TTGGTTTAAT GAATAAGAGT CCACACGAGT GGTTTAATAG CGGTGTGGAT GAACTCTATA TAAATGAGCT TGTAAATAAG CGTTTGGAAG CAAAAAAACA CAAAAATTGG TTATTAGCCG ATCAAATTCG TAACCAGTTA TTAGAGGAAA AAATAATTCT AGAAGATCAA CCTGATGGTA CTACTATTTG GCGAAAAGAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT