RiriA.00113.a
3-oxoacyl-[acyl-carrier-protein] synthase 2
| CENTER ID: | RiriA.00113.a |
| ORGANISM: | Rickettsia rickettsii str. Sheila Smith |
| ASSOCIATED DISEASE: | Rocky Mountain Spotted fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiriA.00113.a.B1.GE36173 | Full length( RiriA.00113.a ) | 1 | 428 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4JGA
DEPOSITED: 2/28/2013
DETERMINATION: XRay
CLONE: RiriA.00113.a.B1.GE36173
PROTEIN: RiriA.00113.a.B1.PS01703
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126985 |
| PATRIC ID: | fig|392021.5.peg.1371 |
| RefSeq: | YP_001495263.1 |
| UniProt: | A0A0H3AY56 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNQRVNKRV VITGLGLVTP VGLNVNSSWK NIVDGVSGIK TITEFDTSKL ACKIAGLIDN SEKDGFKLEN FTQADDINRL SKMDKFIHYG VAAATEAVED SGWLPDDEKS RDRTGLILGS GIGGLKMIED TSIKLYQENN GKVSPFFIPA SLINLLSGLV SIKYGFSGPN QTAVTACSTG AHAIGDAMRM IKHGYADVMI AGGAEAPVTP VGVAGFVAAR ALCTKYNDNP KKASRPWDKD RSGFVMGEGA GVVVLEEYEH ALNRGAKVYG EVIGYGSTGD AYHMTAPHPE GRGAYRAMRD AMLDATITPD MIDYINAHGT STTLGDGIEL AAVQKLFLEA NPKVLMSSTK SSIGHLLGAA GSVEFIFSAL AIRDQIAPPT LNLDTPMDEV NIDLVALKAK KTKIDYVLSN SFGFGGTNAS LVIKSILV
NT Sequence
ATGTCGAATC AAAGAGTAAA TAAAAGAGTA GTTATTACCG GACTTGGACT TGTTACTCCT GTTGGGCTTA ATGTTAACTC GTCTTGGAAA AATATTGTAG ATGGAGTAAG CGGTATAAAA ACTATTACAG AATTTGACAC TTCTAAACTT GCGTGTAAAA TTGCTGGACT TATAGATAAC TCTGAGAAAG ATGGATTTAA ACTTGAAAAT TTCACACAAG CAGATGACAT TAATAGACTA AGTAAAATGG ATAAATTTAT CCATTACGGA GTAGCAGCCG CAACCGAAGC AGTTGAAGAT AGCGGCTGGT TACCCGACGA TGAAAAATCT CGTGATAGAA CCGGCTTGAT ATTAGGTTCA GGAATCGGCG GGCTTAAAAT GATCGAAGAT ACCTCTATCA AACTTTATCA AGAAAATAAC GGCAAAGTTA GTCCTTTCTT TATCCCCGCT TCATTAATTA ATCTTTTATC CGGTCTTGTT TCTATAAAAT ACGGTTTTAG CGGTCCAAAC CAAACGGCAG TAACAGCTTG CTCTACAGGG GCACATGCTA TAGGCGATGC TATGCGTATG ATAAAGCACG GCTATGCCGA TGTTATGATA GCAGGCGGTG CGGAAGCTCC AGTTACACCT GTCGGAGTTG CAGGTTTCGT TGCTGCTAGA GCATTATGTA CTAAATATAA CGATAATCCT AAAAAAGCCT CAAGACCTTG GGATAAAGAC CGCAGCGGCT TTGTTATGGG TGAAGGAGCA GGCGTTGTAG TCTTAGAAGA ATATGAGCAT GCCTTAAATC GAGGGGCTAA AGTTTACGGT GAAGTTATAG GATACGGCTC TACAGGTGAC GCCTATCATA TGACAGCTCC ACATCCTGAA GGTAGAGGAG CTTATAGAGC GATGAGAGAC GCAATGCTAG ATGCAACTAT CACGCCCGAT ATGATTGATT ATATTAATGC ACACGGTACT TCTACTACAC TTGGTGATGG AATAGAATTA GCAGCCGTAC AAAAATTATT TTTAGAAGCG AATCCTAAAG TTTTAATGTC TTCTACTAAA TCATCAATAG GACATTTGCT TGGTGCAGCA GGAAGTGTTG AGTTTATCTT TTCAGCTCTT GCCATTAGAG ACCAGATTGC ACCGCCAACC TTAAACTTAG ATACCCCTAT GGATGAGGTG AATATAGATT TAGTTGCACT GAAAGCTAAG AAAACTAAGA TAGATTACGT GCTTTCCAAC TCGTTCGGAT TCGGTGGTAC TAATGCTAGT TTAGTGATTA AGAGTATACT CGTT
Details for RiriA.00113.a.B1.GE36173
| HARVESTED ON: | 9/26/2012 |
| SEQUENCED ON: | 9/25/2012 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 60 |
Validated AA Sequence
MAHHHHHHMS NQRVNKRVVI TGLGLVTPVG LNVNSSWKNI VDGVSGIKTI TEFDTSKLAC KIAGLIDNSE KDGFKLENFT QADDINRLSK MDKFIHYGVA AATEAVEDSG WLPDDEKSRD RTGLILGSGI GGLKMIEDTS IKLYQENNGK VSPFFIPASL INLLSGLVSI KYGFSGPNQT AVTACSTGAH AIGDAMRMIK HGYADVMIAX GAEAPVTPVG VAXFVAARAL CTKYNDNPKK ASRPWDKDRS GFVMGEXXXL XSXXI
Validated NT Sequence
cnnnnnnana ttttgtttac tttaagaagg aganatacca tggctcacca ccaccaccac catatgtcga atcaaagagt aaataaaaga gtagttatta ccggacttgg acttgttact cctgttgggc ttaatgttaa ctcgtcttgg aaaaatattg tagatggagt aagcggtata aaaactatta cagaatttga cacttctaaa cttgcgtgta aaattgctgg acttatagat aactctgaga aagatggatt taaacttgaa aatttcacac aagcagatga cattaataga ctaagtaaaa tggataaatt tatccattac ggagtagcag ccgcaaccga agcagttgaa gatagcggct ggttacccga cgatgaaaaa tctcgtgata gaaccggctt gatattaggt tcaggaatcg gcgggcttaa aatgatcgaa gatacctcta tcaaacttta tcaagaaaat aacggcaaag ttagtccttt ctttatcccc gcttcattaa ttaatctttt atccggtctt gtttctataa aatacggttt tagcggtcca aaccaaacgg cagtaacagc ttgctctaca ggggcacatg ctataggcga tgctatgcgt atgataaagc acggctatgc cgatgttatg atagcangcg gtgcggaagc tccagttaca cctgtcggag ttgcangttt cgttgctgct agagcattat gtactaaata taacgataat cctaaaaaag cctcaagacc ttgggataaa gaccgcagcg gctttgttat gggtgaanga ncnnngttgn antcttanaa natatgagcn tgccnnaatc naggggctna anttacggtg aanttatann atanngctnt acnnntgann cnnatcatat gacagctccn nncctgannn nnannanctn ananagngat gananannca atgntnnnng nantatnang cccganatga tnntnnnnnn ntgnnnnnnn actncnacnn cnnnnnngnn gnnnnnnnng cancgnannn nnnnnttnnn agcnaatnnn nntnnnangn cnnnnnctna nnntnnanng nnntnnntgn ncnncnnnnn nnnnnnntnn nnnntnnnnn nnnnnnnnnn cnncnnnnnn nntnnnnncc cnnngnnnnn nnnnnnnnnn ntnnng
Expected Protein Sequence
MAHHHHHHMS NQRVNKRVVI TGLGLVTPVG LNVNSSWKNI VDGVSGIKTI TEFDTSKLAC KIAGLIDNSE KDGFKLENFT QADDINRLSK MDKFIHYGVA AATEAVEDSG WLPDDEKSRD RTGLILGSGI GGLKMIEDTS IKLYQENNGK VSPFFIPASL INLLSGLVSI KYGFSGPNQT AVTACSTGAH AIGDAMRMIK HGYADVMIAG GAEAPVTPVG VAGFVAARAL CTKYNDNPKK ASRPWDKDRS GFVMGEGAGV VVLEEYEHAL NRGAKVYGEV IGYGSTGDAY HMTAPHPEGR GAYRAMRDAM LDATITPDMI DYINAHGTST TLGDGIELAA VQKLFLEANP KVLMSSTKSS IGHLLGAAGS VEFIFSALAI RDQIAPPTLN LDTPMDEVNI DLVALKAKKT KIDYVLSNSF GFGGTNASLV IKSILV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga atcaaagagt aaataaaaga gtagttatta ccggacttgg acttgttact cctgttgggc ttaatgttaa ctcgtcttgg aaaaatattg tagatggagt aagcggtata aaaactatta cagaatttga cacttctaaa cttgcgtgta aaattgctgg acttatagat aactctgaga aagatggatt taaacttgaa aatttcacac aagcagatga cattaataga ctaagtaaaa tggataaatt tatccattac ggagtagcag ccgcaaccga agcagttgaa gatagcggct ggttacccga cgatgaaaaa tctcgtgata gaaccggctt gatattaggt tcaggaatcg gcgggcttaa aatgatcgaa gatacctcta tcaaacttta tcaagaaaat aacggcaaag ttagtccttt ctttatcccc gcttcattaa ttaatctttt atccggtctt gtttctataa aatacggttt tagcggtcca aaccaaacgg cagtaacagc ttgctctaca ggggcacatg ctataggcga tgctatgcgt atgataaagc acggctatgc cgatgttatg atagcaggcg gtgcggaagc tccagttaca cctgtcggag ttgcaggttt cgttgctgct agagcattat gtactaaata taacgataat cctaaaaaag cctcaagacc ttgggataaa gaccgcagcg gctttgttat gggtgaagga gcaggcgttg tagtcttaga agaatatgag catgccttaa atcgaggggc taaagtttac ggtgaagtta taggatacgg ctctacaggt gacgcctatc atatgacagc tccacatcct gaaggtagag gagcttatag agcgatgaga gacgcaatgc tagatgcaac tatcacgccc gatatgattg attatattaa tgcacacggt acttctacta cacttggtga tggaatagaa ttagcagccg tacaaaaatt atttttagaa gcgaatccta aagttttaat gtcttctact aaatcatcaa taggacattt gcttggtgca gcaggaagtg ttgagtttat cttttcagct cttgccatta gagaccagat tgcaccgcca accttaaact tagatacccc tatggatgag gtgaatatag atttagttgc actgaaagct aagaaaacta agatagatta cgtgctttcc aactcgttcg gattcggtgg tactaatgct agtttagtga ttaagagtat actcgtttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at