RiriA.00102.a

2-dehydro-3-deoxyphosphooctonate aldolase

CENTER ID:	RiriA.00102.a
ORGANISM:	Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE:	Rocky Mountain Spotted fever
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiriA.00102.a.B1.GE36172	Full length( RiriA.00102.a )		1	274

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130461
PATRIC ID:	fig\|392021.5.peg.116
RefSeq:	YP_001494206.1
UniProt:	A8GQM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQKVVKLNNI KIGNDLPFVL ITGPCQIEGK DHALFMAEKL VKLTSKLEIP FIYKSSFDKA NRTSVHGIRG VGIEKGLEIL SKVKSEFDCP IVTDVHSESQ CTATAEVADI LQIPAFLCRQ TDLLQAAAKT GKIVKVKKGQ FLAPWDMKNV QTKLETFGVK DILFTERGAC FGYNNLVSDM RSLAIMAELN VPVVFDATHS VQQPGGLGGS TGGERKYVEL LAKAATSVGI AGMYMEVHQD PDNAPSDGPC MMKLDNLESI LIKLKKYDKI TKEK

NT Sequence

ATGCAAAAAG TAGTAAAACT TAACAATATC AAAATAGGAA ATGATTTACC GTTTGTTCTA ATTACAGGGC CTTGTCAGAT TGAAGGAAAA GATCATGCGT TGTTTATGGC AGAAAAACTT GTTAAATTAA CTAGCAAGCT TGAGATTCCA TTTATTTATA AATCTTCTTT TGATAAAGCT AATAGAACTT CCGTACATGG GATTAGAGGG GTTGGTATTG AGAAAGGTTT AGAAATTTTA TCAAAAGTAA AATCTGAATT CGATTGCCCT ATTGTTACTG ATGTTCATTC AGAGAGTCAA TGTACTGCAA CAGCTGAAGT AGCAGATATT TTACAAATTC CGGCATTTTT ATGTAGACAA ACCGACTTGC TTCAAGCTGC AGCAAAAACA GGTAAAATAG TAAAAGTAAA AAAAGGACAG TTTCTAGCTC CGTGGGATAT GAAAAATGTA CAAACAAAAC TAGAAACTTT TGGAGTAAAG GATATATTAT TTACAGAGCG AGGAGCATGT TTTGGCTATA ATAACTTGGT ATCTGATATG CGTAGTCTTG CAATAATGGC AGAGTTAAAT GTACCTGTAG TTTTTGATGC CACACATTCC GTTCAGCAAC CGGGTGGGCT AGGAGGCAGT ACCGGCGGAG AAAGAAAATA TGTAGAGTTA CTTGCAAAGG CAGCAACTTC TGTAGGTATC GCCGGTATGT ATATGGAAGT ACATCAAGAC CCCGATAACG CTCCAAGCGA TGGTCCATGT ATGATGAAGT TGGATAATCT AGAGTCTATT CTTATCAAAC TAAAAAAATA TGATAAAATT ACAAAAGAAA AA

Details for RiriA.00102.a.B1.GE36172

HARVESTED ON:	9/26/2012
SEQUENCED ON:	9/25/2012
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMQ KVVKLNNIKI GNDLPFVLIT GPCQIEGKDH ALFMAEKLVK LTSKLEIPFI YKSSFDKANR TSVHGIRGVG IEKGLEILSK VKSEFDCPIV TDVHSESQCT ATAEVADILQ IPAFLCRQTD LLQAAAKTGK IVKVKKGQFL APWDMKNVQT KLETFGVKDI LFTERGACFG YNNLVSDMRS LAIMAELNVP VVFDATHSVQ QPGGLGGSTG GXRKYVELLA KAATSVXIAG MYMEVHQDPD NAPSDGPCMM KLDNLESILI KLKNMIKLQR K

Validated NT Sequence

attttgnnnn nnttaagaag gagatatacc atggctcacc accaccacca ccatatgcaa aaagtagtaa aacttaacaa tatcaaaata ggaaatgatt taccgtttgt tctaattaca gggccttgtc agattgaagg aaaagatcat gcgttgttta tggcagaaaa acttgttaaa ttaactagca agcttgagat tccatttatt tataaatctt cttttgataa agctaataga acttccgtac atgggattag aggggttggt attgagaaag gtttagaaat tttatcaaaa gtaaaatctg aattcgattg ccctattgtt actgatgttc attcagagag tcaatgtact gcaacagctg aagtagcaga tattttacaa attccggcat ttttatgtag acaaaccgac ttgcttcaag ctgcagcaaa aacaggtaaa atagtaaaag taaaaaaagg acagtttcta gctccgtggg atatgaaaaa tgtacaaaca aaactagaaa cttttggagt aaaggatata ttatttacag agcgaggagc atgttttggc tataataact tggtatctga tatgcgtagt cttgcaataa tggcagagtt aaatgtacct gtagtttttg atgccacaca ttccgttcag caaccgggtg ggctaggagg cagtaccggc gganaaagaa aatatgtaga gttacttgca aaggcagcaa cttctgtang tatcgccggt atgtatatgg aagtacatca agaccccgat aacgctccaa gcgatggtcc atgtatgatg aagttggata atctagagtc tattcttatc aaactaaaaa atatgataaa attacaaaga aaataantga ntaanatnnn nnnnnnnnnn nnccccccnn annnnnnnnn nnnnnnaaaa aaaaanaaan nnnnnnnnnn nnannnacnt nnnnnnnnnn nnnnna

Expected Protein Sequence

MAHHHHHHMQ KVVKLNNIKI GNDLPFVLIT GPCQIEGKDH ALFMAEKLVK LTSKLEIPFI YKSSFDKANR TSVHGIRGVG IEKGLEILSK VKSEFDCPIV TDVHSESQCT ATAEVADILQ IPAFLCRQTD LLQAAAKTGK IVKVKKGQFL APWDMKNVQT KLETFGVKDI LFTERGACFG YNNLVSDMRS LAIMAELNVP VVFDATHSVQ QPGGLGGSTG GERKYVELLA KAATSVGIAG MYMEVHQDPD NAPSDGPCMM KLDNLESILI KLKKYDKITK EK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa aagtagtaaa acttaacaat atcaaaatag gaaatgattt accgtttgtt ctaattacag ggccttgtca gattgaagga aaagatcatg cgttgtttat ggcagaaaaa cttgttaaat taactagcaa gcttgagatt ccatttattt ataaatcttc ttttgataaa gctaatagaa cttccgtaca tgggattaga ggggttggta ttgagaaagg tttagaaatt ttatcaaaag taaaatctga attcgattgc cctattgtta ctgatgttca ttcagagagt caatgtactg caacagctga agtagcagat attttacaaa ttccggcatt tttatgtaga caaaccgact tgcttcaagc tgcagcaaaa acaggtaaaa tagtaaaagt aaaaaaagga cagtttctag ctccgtggga tatgaaaaat gtacaaacaa aactagaaac ttttggagta aaggatatat tatttacaga gcgaggagca tgttttggct ataataactt ggtatctgat atgcgtagtc ttgcaataat ggcagagtta aatgtacctg tagtttttga tgccacacat tccgttcagc aaccgggtgg gctaggaggc agtaccggcg gagaaagaaa atatgtagag ttacttgcaa aggcagcaac ttctgtaggt atcgccggta tgtatatgga agtacatcaa gaccccgata acgctccaag cgatggtcca tgtatgatga agttggataa tctagagtct attcttatca aactaaaaaa atatgataaa attacaaaag aaaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat