RiriA.00081.a

Putative uncharacterized protein

CENTER ID: RiriA.00081.a
ORGANISM: Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE: Rocky Mountain Spotted fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.00081.a.B1.GE35616 Full length( RiriA.00081.a ) 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiriA.00081.a.B1.PS01604 Full length( RiriA.00081.a ) 1 372

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|392021.5.peg.858
RefSeq: YP_001494848.1
UniProt: A0A0H3AXN5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIYLDHNATT FIDPRVKEFI ISLMDKALNP SSAHSSGRFA KNLIETVRAQ IATALGITLS SREYDITFTS SGTEGNNLIM KNFYDGDIFI SAIEHLSIYN HINYAPNIKV ISVNTQGLVD LEHLEELLAQ SNTSKKLVSI IMANNESGVL QDIAEIGKIT KKYEAKFHSD LVQGFGRIPI NIKALGLDFA TISGHKIGGG QGGAALISSS NFQVTPMIIG GGQEKSVRSG TENVLAIAGF GLASALRTDN ISENYIKIKK LQENLEQKLK KYPNVNIVSN NVARLPNTTL ITIPNTDAQA KLIGFDLHNI CVSSGSACSS GKISKSHVLT NMGVGEEEAK SSIRISLSHT NTVSDIEAFI EAFEEIYDVI PS
NT Sequence
ATGATATATT TAGACCATAA TGCTACTACT TTTATCGACC CTAGAGTTAA GGAATTCATA ATAAGTTTAA TGGACAAGGC GCTTAACCCT TCATCAGCAC ATAGCTCAGG TAGATTTGCT AAGAATCTCA TAGAAACGGT ACGCGCACAA ATAGCGACGG CTCTTGGTAT AACCTTATCA TCTAGAGAAT ATGATATTAC TTTTACTTCA TCAGGAACTG AAGGTAATAA TTTAATAATG AAAAATTTTT ATGACGGCGA TATTTTTATT TCAGCTATTG AACATTTATC GATCTATAAC CATATAAACT ACGCTCCAAA TATTAAAGTT ATAAGTGTTA ATACGCAAGG GCTAGTTGAT TTAGAACATT TAGAAGAGTT GTTAGCTCAA AGTAATACTA GTAAAAAACT AGTTTCCATA ATAATGGCTA ATAATGAAAG CGGAGTTTTA CAAGATATAG CTGAGATAGG TAAAATAACT AAAAAATATG AGGCAAAATT TCATAGCGAT TTAGTGCAAG GTTTTGGCAG AATACCAATA AATATCAAAG CATTAGGATT AGATTTTGCT ACAATTTCAG GGCATAAAAT AGGAGGGGGG CAGGGTGGTG CTGCTTTAAT TTCTAGCTCT AATTTTCAAG TTACTCCAAT GATTATAGGA GGAGGGCAGG AAAAATCAGT ACGCTCAGGC ACTGAAAATG TTTTAGCAAT TGCAGGATTT GGTTTAGCTT CTGCATTGAG GACAGACAAT ATCTCAGAAA ATTATATAAA AATCAAAAAG TTACAAGAAA ATTTAGAGCA AAAATTAAAA AAATATCCTA ATGTAAATAT TGTTAGTAAT AACGTAGCTA GGTTGCCTAA TACTACCTTA ATTACTATAC CAAATACGGA TGCACAAGCG AAATTAATCG GGTTTGATTT ACATAATATT TGCGTAAGTT CCGGTTCTGC TTGTTCATCA GGAAAAATAT CTAAATCACA TGTATTAACC AATATGGGTG TAGGGGAAGA AGAAGCAAAA TCTTCGATTA GGATATCTTT AAGCCATACT AATACGGTAA GTGATATAGA GGCTTTTATA GAGGCTTTTG AGGAGATTTA CGATGTCATT CCTAGC
Details for RiriA.00081.a.B1.GE35616
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MIYLDHNATT FIDPRVKEFI ISLMDKALNP SSAHSSGRFA KNLIETVRAQ IATALGITLS SREYDITFTS SGTEGNNLIM KNFYDGDIFI SAIEHLSIYN HINYAPNIKV ISVNTQGLVD LEHLEELLAQ SNTSKKLVSI IMANNESGVL QDIAEIGKIT KKYEAKFHSD LVQGFGRIPI NIKALGLDFA TISGHKIGGG QGGAALISSS NFQVTPMIIG GGQEKSVRSG TENVLAIAGF GLASALRTDN ISENYIKIKK LQENLEQKLK KYPNVNIVSN NVARLPNTTL ITIPNTDAQA KLIGFDLHNI CVSSGSACSS GKISKSHVLT NMGVGEEEAK SSIRISLSHT NTVSDIEAFI EA
Validated NT Sequence
aaagcctcta taaaagcctc tatatcactt accgtattag tatggcttaa agatatccta atcgaagatt ttgcttcttc ttcccctaca cccatattgg ttaatacatg tgatttagat atttttcctg atgaacaagc agaaccggaa cttacgcaaa tattatgtaa atcaaacccg attaatttcg cttgtgcatc cgtatttggt atagtaatta aggtagtatt aggcaaccta gctacgttat tactaacaat atttacatta ggatattttt ttaatttttg ctctaaattt tcttgtaact ttttgatttt tatataattt tctgagatat tgtctgtcct caatgcagaa gctaaaccaa atcctgcaat tgctaaaaca ttttcagtgc ctgagcgtac tgatttttcc tgccctcctc ctataatcat tggagtaact tgaaaattag agctagaaat taaagcagca ccaccctgcc cccctcctat tttatgccct gaaattgtag caaaatctaa tcctaatgct ttgatattta ttggtattct gccaaaacct tgcactaaat cgctatgaaa ttttgcctca tattttttag ttattttacc tatctcagct atatcttgta aaactccgct ttcattatta gccattatta tggaaactag ttttttacta gtattacttt gagctaacaa ctcttctaaa tgttctaaat caactagccc ttgcgtatta acacttataa ctttaatatt tggagcgtag tttatatggt tatagatcga taaatgttca atagctgaaa taaaaatatc gccgtcataa aaatttttca ttattaaatt attaccttca gttcctgatg aagtaaaagt aatatcatat tctctagatg ataaggttat accaagagcc gtcgctattt gtgcgcgtac cgtttctatg agattcttag caaatctacc tgagctatgt gctgatgaag ggttaagcgc cttgtccatt aaacttatta tgaattcctt aactctaggg tcgataaaag tagtagcatt atggtctaaa tatatcatat ggtggtggtg gtggtgagc
Expected Protein Sequence
MAHHHHHHMI YLDHNATTFI DPRVKEFIIS LMDKALNPSS AHSSGRFAKN LIETVRAQIA TALGITLSSR EYDITFTSSG TEGNNLIMKN FYDGDIFISA IEHLSIYNHI NYAPNIKVIS VNTQGLVDLE HLEELLAQSN TSKKLVSIIM ANNESGVLQD IAEIGKITKK YEAKFHSDLV QGFGRIPINI KALGLDFATI SGHKIGGGQG GAALISSSNF QVTPMIIGGG QEKSVRSGTE NVLAIAGFGL ASALRTDNIS ENYIKIKKLQ ENLEQKLKKY PNVNIVSNNV ARLPNTTLIT IPNTDAQAKL IGFDLHNICV SSGSACSSGK ISKSHVLTNM GVGEEEAKSS IRISLSHTNT VSDIEAFIEA FEEIYDVIPS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatat atttagacca taatgctact acttttatcg accctagagt taaggaattc ataataagtt taatggacaa ggcgcttaac ccttcatcag cacatagctc aggtagattt gctaagaatc tcatagaaac ggtacgcgca caaatagcga cggctcttgg tataacctta tcatctagag aatatgatat tacttttact tcatcaggaa ctgaaggtaa taatttaata atgaaaaatt tttatgacgg cgatattttt atttcagcta ttgaacattt atcgatctat aaccatataa actacgctcc aaatattaaa gttataagtg ttaatacgca agggctagtt gatttagaac atttagaaga gttgttagct caaagtaata ctagtaaaaa actagtttcc ataataatgg ctaataatga aagcggagtt ttacaagata tagctgagat aggtaaaata actaaaaaat atgaggcaaa atttcatagc gatttagtgc aaggttttgg cagaatacca ataaatatca aagcattagg attagatttt gctacaattt cagggcataa aataggaggg gggcagggtg gtgctgcttt aatttctagc tctaattttc aagttactcc aatgattata ggaggagggc aggaaaaatc agtacgctca ggcactgaaa atgttttagc aattgcagga tttggtttag cttctgcatt gaggacagac aatatctcag aaaattatat aaaaatcaaa aagttacaag aaaatttaga gcaaaaatta aaaaaatatc ctaatgtaaa tattgttagt aataacgtag ctaggttgcc taatactacc ttaattacta taccaaatac ggatgcacaa gcgaaattaa tcgggtttga tttacataat atttgcgtaa gttccggttc tgcttgttca tcaggaaaaa tatctaaatc acatgtatta accaatatgg gtgtagggga agaagaagca aaatcttcga ttaggatatc tttaagccat actaatacgg taagtgatat agaggctttt atagaggctt ttgaggagat ttacgatgtc attcctagct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for RiriA.00081.a.B1.PS01604
PURIFICATION DATe: 8/21/2012
CONCENTRATION: 35mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MIYLDHNATT FIDPRVKEFI ISLMDKALNP SSAHSSGRFA KNLIETVRAQ IATALGITLS SREYDITFTS SGTEGNNLIM KNFYDGDIFI SAIEHLSIYN HINYAPNIKV ISVNTQGLVD LEHLEELLAQ SNTSKKLVSI IMANNESGVL QDIAEIGKIT KKYEAKFHSD LVQGFGRIPI NIKALGLDFA TISGHKIGGG QGGAALISSS NFQVTPMIIG GGQEKSVRSG TENVLAIAGF GLASALRTDN ISENYIKIKK LQENLEQKLK KYPNVNIVSN NVARLPNTTL ITIPNTDAQA KLIGFDLHNI CVSSGSACSS GKISKSHVLT NMGVGEEEAK SSIRISLSHT NTVSDIEAFI EA
Validated NT Sequence
aaagcctcta taaaagcctc tatatcactt accgtattag tatggcttaa agatatccta atcgaagatt ttgcttcttc ttcccctaca cccatattgg ttaatacatg tgatttagat atttttcctg atgaacaagc agaaccggaa cttacgcaaa tattatgtaa atcaaacccg attaatttcg cttgtgcatc cgtatttggt atagtaatta aggtagtatt aggcaaccta gctacgttat tactaacaat atttacatta ggatattttt ttaatttttg ctctaaattt tcttgtaact ttttgatttt tatataattt tctgagatat tgtctgtcct caatgcagaa gctaaaccaa atcctgcaat tgctaaaaca ttttcagtgc ctgagcgtac tgatttttcc tgccctcctc ctataatcat tggagtaact tgaaaattag agctagaaat taaagcagca ccaccctgcc cccctcctat tttatgccct gaaattgtag caaaatctaa tcctaatgct ttgatattta ttggtattct gccaaaacct tgcactaaat cgctatgaaa ttttgcctca tattttttag ttattttacc tatctcagct atatcttgta aaactccgct ttcattatta gccattatta tggaaactag ttttttacta gtattacttt gagctaacaa ctcttctaaa tgttctaaat caactagccc ttgcgtatta acacttataa ctttaatatt tggagcgtag tttatatggt tatagatcga taaatgttca atagctgaaa taaaaatatc gccgtcataa aaatttttca ttattaaatt attaccttca gttcctgatg aagtaaaagt aatatcatat tctctagatg ataaggttat accaagagcc gtcgctattt gtgcgcgtac cgtttctatg agattcttag caaatctacc tgagctatgt gctgatgaag ggttaagcgc cttgtccatt aaacttatta tgaattcctt aactctaggg tcgataaaag tagtagcatt atggtctaaa tatatcatat ggtggtggtg gtggtgagc
Expressed Protein Sequence
MAHHHHHHMI YLDHNATTFI DPRVKEFIIS LMDKALNPSS AHSSGRFAKN LIETVRAQIA TALGITLSSR EYDITFTSSG TEGNNLIMKN FYDGDIFISA IEHLSIYNHI NYAPNIKVIS VNTQGLVDLE HLEELLAQSN TSKKLVSIIM ANNESGVLQD IAEIGKITKK YEAKFHSDLV QGFGRIPINI KALGLDFATI SGHKIGGGQG GAALISSSNF QVTPMIIGGG QEKSVRSGTE NVLAIAGFGL ASALRTDNIS ENYIKIKKLQ ENLEQKLKKY PNVNIVSNNV ARLPNTTLIT IPNTDAQAKL IGFDLHNICV SSGSACSSGK ISKSHVLTNM GVGEEEAKSS IRISLSHTNT VSDIEAFIEA FEEIYDVIPS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATAT ATTTAGACCA TAATGCTACT ACTTTTATCG ACCCTAGAGT TAAGGAATTC ATAATAAGTT TAATGGACAA GGCGCTTAAC CCTTCATCAG CACATAGCTC AGGTAGATTT GCTAAGAATC TCATAGAAAC GGTACGCGCA CAAATAGCGA CGGCTCTTGG TATAACCTTA TCATCTAGAG AATATGATAT TACTTTTACT TCATCAGGAA CTGAAGGTAA TAATTTAATA ATGAAAAATT TTTATGACGG CGATATTTTT ATTTCAGCTA TTGAACATTT ATCGATCTAT AACCATATAA ACTACGCTCC AAATATTAAA GTTATAAGTG TTAATACGCA AGGGCTAGTT GATTTAGAAC ATTTAGAAGA GTTGTTAGCT CAAAGTAATA CTAGTAAAAA ACTAGTTTCC ATAATAATGG CTAATAATGA AAGCGGAGTT TTACAAGATA TAGCTGAGAT AGGTAAAATA ACTAAAAAAT ATGAGGCAAA ATTTCATAGC GATTTAGTGC AAGGTTTTGG CAGAATACCA ATAAATATCA AAGCATTAGG ATTAGATTTT GCTACAATTT CAGGGCATAA AATAGGAGGG GGGCAGGGTG GTGCTGCTTT AATTTCTAGC TCTAATTTTC AAGTTACTCC AATGATTATA GGAGGAGGGC AGGAAAAATC AGTACGCTCA GGCACTGAAA ATGTTTTAGC AATTGCAGGA TTTGGTTTAG CTTCTGCATT GAGGACAGAC AATATCTCAG AAAATTATAT AAAAATCAAA AAGTTACAAG AAAATTTAGA GCAAAAATTA AAAAAATATC CTAATGTAAA TATTGTTAGT AATAACGTAG CTAGGTTGCC TAATACTACC TTAATTACTA TACCAAATAC GGATGCACAA GCGAAATTAA TCGGGTTTGA TTTACATAAT ATTTGCGTAA GTTCCGGTTC TGCTTGTTCA TCAGGAAAAA TATCTAAATC ACATGTATTA ACCAATATGG GTGTAGGGGA AGAAGAAGCA AAATCTTCGA TTAGGATATC TTTAAGCCAT ACTAATACGG TAAGTGATAT AGAGGCTTTT ATAGAGGCTT TTGAGGAGAT TTACGATGTC ATTCCTAGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT