SSGCID: Target Page

RiriA.00057.a

ABC transporter ATP-binding protein

CENTER ID:	RiriA.00057.a
ORGANISM:	Rickettsia rickettsii str. Sheila Smith
ASSOCIATED DISEASE:	Rocky Mountain Spotted fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiriA.00057.a.B1.GE36168	Full length( RiriA.00057.a )		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|392021.5.peg.780
RefSeq:	YP_001494774.1
UniProt:	A0A0H3AUS4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDSLHVKNIS KSYKKRNILT DISLNIKQGE IVGLFGPNGA GKTTCFNIII GLMKLDSGQL LLNDINITNL PIYLRARLGL GYLLQEPSIF RELSVEDNIK AVVEISNNDK EVIEQKTHNL LEQFSIVHLK DLSAASLSGG ERRRLEIARA LAIEPKFIML DEPLAGIDPL AISDIKNLIT YLREFNIGIL ITDHNVRDTL DIVDRAYVIF EGKVLLEGSA KEIANSKKVK EVYLGESFSF

NT Sequence

ATGGACAGCT TACACGTTAA AAATATATCA AAATCCTACA AAAAAAGGAA TATATTAACT GATATATCTC TGAATATAAA ACAAGGAGAG ATAGTTGGTT TATTCGGTCC GAATGGAGCC GGTAAAACAA CTTGCTTTAA CATTATTATC GGTTTAATGA AACTGGACTC AGGGCAATTA CTTTTAAATG ATATCAATAT TACCAACTTG CCTATTTATT TACGAGCAAG GCTTGGGCTC GGCTATCTTC TTCAAGAACC TTCGATATTT CGGGAATTGT CGGTTGAAGA TAATATTAAG GCTGTAGTTG AGATATCTAA CAATGATAAA GAAGTAATTG AACAAAAAAC CCATAATTTG TTAGAGCAGT TTTCGATAGT GCATTTAAAA GATTTATCCG CTGCTAGCTT ATCAGGCGGT GAAAGGCGTA GGCTTGAGAT TGCACGTGCA CTTGCAATAG AGCCTAAATT TATCATGCTT GATGAACCGC TTGCCGGTAT TGACCCGCTT GCTATTTCCG ATATTAAAAA TCTCATTACT TATTTACGTG AATTTAATAT AGGTATATTA ATTACCGACC ATAATGTACG TGATACTCTA GATATAGTCG ACCGTGCTTA TGTTATTTTT GAAGGTAAAG TATTATTAGA AGGAAGTGCT AAGGAAATAG CAAATAGCAA GAAAGTTAAG GAAGTGTATT TAGGTGAGAG CTTTAGTTTC

Details for RiriA.00057.a.B1.GE36168

HARVESTED ON:	9/26/2012
SEQUENCED ON:	9/25/2012
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD SLHVKNISKS YKKRNILTDI SLNIKQGEIV GLFGPNGAGK TTCFNIIIGL MKLDSGQLLL NDINITNLPI YLRARLGLGY LLQEPSIFRE LSVEDNIKAV VEISNNDKEV IEQKTHNLLE QFSIVHLKDL SAASLSGGER RRLEIARALA IEPKFIMLDE PLAGIDPLAI SDIKNLITYL REFNIGILIT DHNVRDTLDI VDRAYVIFEG KVLLEGSAKE IAXSKKVKEV YLGESFSF

Validated NT Sequence

tttntttnct ttaagaagga ganataccat ggctcaccac caccaccacc atatggacag cttacacgtt aaaaatatat caaaatccta caaaaaaagg aatatattaa ctgatatatc tctgaatata aaacaaggag agatagttgg tttattcggt ccgaatggag ccggtaaaac aacttgcttt aacattatta tcggtttaat gaaactggac tcagggcaat tacttttaaa tgatatcaat attaccaact tgcctattta tttacgagca aggcttgggc tcggctatct tcttcaagaa ccttcgatat ttcgggaatt gtcggttgaa gataatatta aggctgtagt tgagatatct aacaatgata aagaagtaat tgaacaaaaa acccataatt tgttagagca gttttcgata gtgcatttaa aagatttatc cgctgctagc ttatcaggcg gtgaaaggcg taggcttgag attgcacgtg cacttgcaat agagcctaaa tttatcatgc ttgatgaacc gcttgccggt attgacccgc ttgctatttc cgatattaaa aatctcatta cttatttacg tgaatttaat ataggtatat taattaccga ccataatgta cgtgatactc tagatatagt cgaccgtgct tatgttattt ttgaaggtaa agtattatta gaaggaagtg ctaaggaaat agcanatagc aagaaagtta aggaagtgta tttaggtgag agctttagtt tctaagtgag taagatagga tccggctgnn nnnnnccccc cnnnnnnnnn nnnnnnnnna naaaanannn cnnnnnnnnn nannnnnnnn nnnnnnnnnn nnnnnnnnnn nntnancnnn tnnnnnnnnn nnnnnnnnnn nnnnngnnnn nnnnctnnng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca gcttacacgt taaaaatata tcaaaatcct acaaaaaaag gaatatatta actgatatat ctctgaatat aaaacaagga gagatagttg gtttattcgg tccgaatgga gccggtaaaa caacttgctt taacattatt atcggtttaa tgaaactgga ctcagggcaa ttacttttaa atgatatcaa tattaccaac ttgcctattt atttacgagc aaggcttggg ctcggctatc ttcttcaaga accttcgata tttcgggaat tgtcggttga agataatatt aaggctgtag ttgagatatc taacaatgat aaagaagtaa ttgaacaaaa aacccataat ttgttagagc agttttcgat agtgcattta aaagatttat ccgctgctag cttatcaggc ggtgaaaggc gtaggcttga gattgcacgt gcacttgcaa tagagcctaa atttatcatg cttgatgaac cgcttgccgg tattgacccg cttgctattt ccgatattaa aaatctcatt acttatttac gtgaatttaa tataggtata ttaattaccg accataatgt acgtgatact ctagatatag tcgaccgtgc ttatgttatt tttgaaggta aagtattatt agaaggaagt gctaaggaaa tagcaaatag caagaaagtt aaggaagtgt atttaggtga gagctttagt ttctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat