RiriA.00008.a
Serine hydroxymethyltransferase
| CENTER ID: | RiriA.00008.a |
| ORGANISM: | Rickettsia rickettsii str. Sheila Smith |
| ASSOCIATED DISEASE: | Rocky Mountain Spotted fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiriA.00008.a.B1.GE36167 | Full length( RiriA.00008.a ) | 1 | 420 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiriA.00008.a.B1.PS01698 | Full length( RiriA.00008.a ) | 1 | 420 |
Structures
4J5U
DEPOSITED: 2/10/2013
DETERMINATION: XRay
CLONE: RiriA.00008.a.B1.GE36167
PROTEIN: RiriA.00008.a.B1.PS01698
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126679 |
| PATRIC ID: | fig|392021.5.peg.1331 |
| RefSeq: | YP_001495222.1 |
| UniProt: | A8GTI9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIFNNNLHE TDKEINEIIK HEKLRQSSVI ELIASENFVS PAVLEAQGAL LTNKYAEGYP SKRFYNGCEE VDKAENLAIE RVKKLFNCKY ANVQPHSGSQ ANQAVYLALL QPGDTVLGMS LDSGGHLTHG AAPNMSGKWF NAVSYSVNKE TYLIDYDEIE RLADLHKPKL LIAGFSAYPR NIDFAKFREI VDKVGAYFMA DIAHIAGLVA TGEHQSPIPY AHAVTSTTHK TLRGPRGGLI LSNDEEIGHK INSALFPGLQ GGPLMHIIAA KAVAFLENLQ PEYKSYIQQV ISNAKALASS LQERGYDILT GGTDNHIVLV DLRKDGITGK LAANSLDRAG ITCNKNAIPF DETSPFITSG IRLGTPACTT RGFKEKDFVL VGHMVADILD GLKNNEDNSA LEQQVLNEVT KLIELFPFYG
NT Sequence
ATGAATATTT TTAATAATAA TTTACACGAA ACGGATAAAG AGATTAATGA AATAATAAAG CATGAAAAGC TACGTCAAAG TAGTGTAATC GAGCTTATTG CATCAGAAAA TTTCGTAAGT CCTGCTGTGC TTGAAGCTCA AGGGGCGCTT CTGACTAACA AATATGCCGA AGGATACCCA AGTAAGCGAT TCTATAACGG TTGCGAGGAA GTAGATAAAG CTGAAAATTT AGCTATAGAA CGAGTAAAAA AACTCTTTAA CTGCAAATAT GCAAATGTCC AACCTCATTC AGGATCACAA GCAAATCAGG CTGTATATCT TGCTTTATTA CAGCCGGGTG ATACGGTTCT TGGTATGTCC TTAGATAGCG GCGGGCATCT AACTCACGGT GCAGCGCCTA ATATGTCTGG TAAATGGTTT AACGCCGTTT CTTATAGCGT AAATAAAGAG ACTTATTTAA TCGATTATGA TGAGATTGAA CGCTTAGCGG ATTTGCATAA ACCAAAATTA CTTATAGCCG GTTTTTCTGC TTATCCTCGT AATATTGATT TTGCAAAATT TAGAGAAATC GTGGATAAAG TAGGAGCTTA CTTTATGGCA GATATTGCTC ATATTGCAGG ACTTGTTGCC ACAGGTGAGC ATCAAAGCCC TATTCCTTAT GCTCACGCTG TTACCTCTAC TACTCATAAA ACACTTAGAG GTCCAAGAGG TGGTTTAATT TTATCTAACG ATGAAGAGAT AGGTCACAAA ATAAATTCTG CATTATTTCC AGGACTACAA GGTGGTCCGT TAATGCATAT AATAGCTGCA AAAGCCGTAG CTTTTTTAGA AAATTTGCAG CCTGAATATA AAAGTTATAT TCAGCAGGTA ATAAGTAACG CTAAAGCTTT AGCAAGTAGC TTGCAAGAAA GAGGATATGA TATATTAACA GGTGGTACCG ATAATCATAT TGTTTTAGTG GATTTGCGTA AAGACGGAAT CACTGGTAAG CTTGCCGCAA ATTCTTTGGA TAGAGCAGGG ATTACGTGTA ATAAGAATGC TATCCCGTTT GATGAAACTT CGCCTTTTAT CACTTCAGGC ATTCGCCTTG GTACTCCCGC ATGTACTACT AGAGGCTTTA AAGAAAAGGA TTTTGTATTA GTCGGTCATA TGGTAGCAGA TATTTTAGAC GGCTTAAAAA ATAATGAAGA TAATAGCGCT CTTGAGCAGC AAGTATTAAA TGAAGTGACA AAATTAATTG AATTATTTCC GTTTTATGGC
Details for RiriA.00008.a.B1.GE36167
| HARVESTED ON: | 9/26/2012 |
| SEQUENCED ON: | 9/25/2012 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 67 |
Validated AA Sequence
MSLDSGGHLT HGAAPNMSGK WFNAVSYSVN KETYLIDYDE IERLADLHKP KLLIAGFSAY PRNIDFAKFR EIVDKVGAYF MADIAHIAGL VATGEHQSPI PYAHAVTSTT HKTLRGPRGG LILSNDEEIG HKINSALFPG LQGGPLMHII AAKAVAFLEN LQPEYKSYIQ QVISNAKALA SSLQERGYDI LTGGTDNHIV LVDLRKDGIT GKLAANSLDR AGITCNKNAI PFDETSPFIT SGIRLGTPAC TTRGFKEKDF VLVGHMVADI LDGLKNNEDN SALEQQVL
Validated NT Sequence
atttaatact tgctgctcaa gagcgctatt atcttcatta ttttttaagc cgtctaaaat atctgctacc atatgaccga ctaatacaaa atccttttct ttaaagcctc tagtagtaca tgcgggagta ccaaggcgaa tgcctgaagt gataaaaggc gaagtttcat caaacgggat agcattctta ttacacgtaa tccctgctct atccaaagaa tttgcggcaa gcttaccagt gattccgtct ttacgcaaat ccactaaaac aatatgatta tcggtaccac ctgttaatat atcatatcct ctttcttgca agctacttgc taaagcttta gcgttactta ttacctgctg aatataactt ttatattcag gctgcaaatt ttctaaaaaa gctacggctt ttgcagctat tatatgcatt aacggaccac cttgtagtcc tggaaataat gcagaattta ttttgtgacc tatctcttca tcgttagata aaattaaacc acctcttgga cctctaagtg ttttatgagt agtagaggta acagcgtgag cataaggaat agggctttga tgctcacctg tggcaacaag tcctgcaata tgagcaatat ctgccataaa gtaagctcct actttatcca cgatttctct aaattttgca aaatcaatat tacgaggata agcagaaaaa ccggctataa gtaattttgg tttatgcaaa tccgctaagc gttcaatctc atcataatcg attaaataag tctctttatt tacgctataa gaaacggcgt taaaccattt accagacata ttaggcgctg caccgtgagt tagatgcccg ccgctatcta aggacatacc aagaaccgta tcacccggct gtaataaagc aagatataca gcctgatttg cttgtgatcc tgaatgaggt tggacatttg catatttgca gttaaagagt ttttttactc gttctatagc taaattttca gctttatcta cttcctcgca accgttatag aatcgcttac ttgggtatcc ttcggcatat ttgttagtca gaagcgcccc ttgagcttca agcacagcag gacttacgaa attttctgat gcaataagct cgattacact actttgacgt agcttttcat gctttattat ttcattaatc tctttatccg tttcgtgtaa attattatta aaaatatt
Expected Protein Sequence
MAHHHHHHMN IFNNNLHETD KEINEIIKHE KLRQSSVIEL IASENFVSPA VLEAQGALLT NKYAEGYPSK RFYNGCEEVD KAENLAIERV KKLFNCKYAN VQPHSGSQAN QAVYLALLQP GDTVLGMSLD SGGHLTHGAA PNMSGKWFNA VSYSVNKETY LIDYDEIERL ADLHKPKLLI AGFSAYPRNI DFAKFREIVD KVGAYFMADI AHIAGLVATG EHQSPIPYAH AVTSTTHKTL RGPRGGLILS NDEEIGHKIN SALFPGLQGG PLMHIIAAKA VAFLENLQPE YKSYIQQVIS NAKALASSLQ ERGYDILTGG TDNHIVLVDL RKDGITGKLA ANSLDRAGIT CNKNAIPFDE TSPFITSGIR LGTPACTTRG FKEKDFVLVG HMVADILDGL KNNEDNSALE QQVLNEVTKL IELFPFYG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata tttttaataa taatttacac gaaacggata aagagattaa tgaaataata aagcatgaaa agctacgtca aagtagtgta atcgagctta ttgcatcaga aaatttcgta agtcctgctg tgcttgaagc tcaaggggcg cttctgacta acaaatatgc cgaaggatac ccaagtaagc gattctataa cggttgcgag gaagtagata aagctgaaaa tttagctata gaacgagtaa aaaaactctt taactgcaaa tatgcaaatg tccaacctca ttcaggatca caagcaaatc aggctgtata tcttgcttta ttacagccgg gtgatacggt tcttggtatg tccttagata gcggcgggca tctaactcac ggtgcagcgc ctaatatgtc tggtaaatgg tttaacgccg tttcttatag cgtaaataaa gagacttatt taatcgatta tgatgagatt gaacgcttag cggatttgca taaaccaaaa ttacttatag ccggtttttc tgcttatcct cgtaatattg attttgcaaa atttagagaa atcgtggata aagtaggagc ttactttatg gcagatattg ctcatattgc aggacttgtt gccacaggtg agcatcaaag ccctattcct tatgctcacg ctgttacctc tactactcat aaaacactta gaggtccaag aggtggttta attttatcta acgatgaaga gataggtcac aaaataaatt ctgcattatt tccaggacta caaggtggtc cgttaatgca tataatagct gcaaaagccg tagctttttt agaaaatttg cagcctgaat ataaaagtta tattcagcag gtaataagta acgctaaagc tttagcaagt agcttgcaag aaagaggata tgatatatta acaggtggta ccgataatca tattgtttta gtggatttgc gtaaagacgg aatcactggt aagcttgccg caaattcttt ggatagagca gggattacgt gtaataagaa tgctatcccg tttgatgaaa cttcgccttt tatcacttca ggcattcgcc ttggtactcc cgcatgtact actagaggct ttaaagaaaa ggattttgta ttagtcggtc atatggtagc agatatttta gacggcttaa aaaataatga agataatagc gctcttgagc agcaagtatt aaatgaagtg acaaaattaa ttgaattatt tccgttttat ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for RiriA.00008.a.B1.PS01698
| PURIFICATION DATe: | 12/11/2012 |
| CONCENTRATION: | 22.4mg/ml |
| OBSERVED MW: | 47kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 67 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSLDSGGHLT HGAAPNMSGK WFNAVSYSVN KETYLIDYDE IERLADLHKP KLLIAGFSAY PRNIDFAKFR EIVDKVGAYF MADIAHIAGL VATGEHQSPI PYAHAVTSTT HKTLRGPRGG LILSNDEEIG HKINSALFPG LQGGPLMHII AAKAVAFLEN LQPEYKSYIQ QVISNAKALA SSLQERGYDI LTGGTDNHIV LVDLRKDGIT GKLAANSLDR AGITCNKNAI PFDETSPFIT SGIRLGTPAC TTRGFKEKDF VLVGHMVADI LDGLKNNEDN SALEQQVL
Validated NT Sequence
atttaatact tgctgctcaa gagcgctatt atcttcatta ttttttaagc cgtctaaaat atctgctacc atatgaccga ctaatacaaa atccttttct ttaaagcctc tagtagtaca tgcgggagta ccaaggcgaa tgcctgaagt gataaaaggc gaagtttcat caaacgggat agcattctta ttacacgtaa tccctgctct atccaaagaa tttgcggcaa gcttaccagt gattccgtct ttacgcaaat ccactaaaac aatatgatta tcggtaccac ctgttaatat atcatatcct ctttcttgca agctacttgc taaagcttta gcgttactta ttacctgctg aatataactt ttatattcag gctgcaaatt ttctaaaaaa gctacggctt ttgcagctat tatatgcatt aacggaccac cttgtagtcc tggaaataat gcagaattta ttttgtgacc tatctcttca tcgttagata aaattaaacc acctcttgga cctctaagtg ttttatgagt agtagaggta acagcgtgag cataaggaat agggctttga tgctcacctg tggcaacaag tcctgcaata tgagcaatat ctgccataaa gtaagctcct actttatcca cgatttctct aaattttgca aaatcaatat tacgaggata agcagaaaaa ccggctataa gtaattttgg tttatgcaaa tccgctaagc gttcaatctc atcataatcg attaaataag tctctttatt tacgctataa gaaacggcgt taaaccattt accagacata ttaggcgctg caccgtgagt tagatgcccg ccgctatcta aggacatacc aagaaccgta tcacccggct gtaataaagc aagatataca gcctgatttg cttgtgatcc tgaatgaggt tggacatttg catatttgca gttaaagagt ttttttactc gttctatagc taaattttca gctttatcta cttcctcgca accgttatag aatcgcttac ttgggtatcc ttcggcatat ttgttagtca gaagcgcccc ttgagcttca agcacagcag gacttacgaa attttctgat gcaataagct cgattacact actttgacgt agcttttcat gctttattat ttcattaatc tctttatccg tttcgtgtaa attattatta aaaatatt
Expressed Protein Sequence
MAHHHHHHMN IFNNNLHETD KEINEIIKHE KLRQSSVIEL IASENFVSPA VLEAQGALLT NKYAEGYPSK RFYNGCEEVD KAENLAIERV KKLFNCKYAN VQPHSGSQAN QAVYLALLQP GDTVLGMSLD SGGHLTHGAA PNMSGKWFNA VSYSVNKETY LIDYDEIERL ADLHKPKLLI AGFSAYPRNI DFAKFREIVD KVGAYFMADI AHIAGLVATG EHQSPIPYAH AVTSTTHKTL RGPRGGLILS NDEEIGHKIN SALFPGLQGG PLMHIIAAKA VAFLENLQPE YKSYIQQVIS NAKALASSLQ ERGYDILTGG TDNHIVLVDL RKDGITGKLA ANSLDRAGIT CNKNAIPFDE TSPFITSGIR LGTPACTTRG FKEKDFVLVG HMVADILDGL KNNEDNSALE QQVLNEVTKL IELFPFYG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA TTTTTAATAA TAATTTACAC GAAACGGATA AAGAGATTAA TGAAATAATA AAGCATGAAA AGCTACGTCA AAGTAGTGTA ATCGAGCTTA TTGCATCAGA AAATTTCGTA AGTCCTGCTG TGCTTGAAGC TCAAGGGGCG CTTCTGACTA ACAAATATGC CGAAGGATAC CCAAGTAAGC GATTCTATAA CGGTTGCGAG GAAGTAGATA AAGCTGAAAA TTTAGCTATA GAACGAGTAA AAAAACTCTT TAACTGCAAA TATGCAAATG TCCAACCTCA TTCAGGATCA CAAGCAAATC AGGCTGTATA TCTTGCTTTA TTACAGCCGG GTGATACGGT TCTTGGTATG TCCTTAGATA GCGGCGGGCA TCTAACTCAC GGTGCAGCGC CTAATATGTC TGGTAAATGG TTTAACGCCG TTTCTTATAG CGTAAATAAA GAGACTTATT TAATCGATTA TGATGAGATT GAACGCTTAG CGGATTTGCA TAAACCAAAA TTACTTATAG CCGGTTTTTC TGCTTATCCT CGTAATATTG ATTTTGCAAA ATTTAGAGAA ATCGTGGATA AAGTAGGAGC TTACTTTATG GCAGATATTG CTCATATTGC AGGACTTGTT GCCACAGGTG AGCATCAAAG CCCTATTCCT TATGCTCACG CTGTTACCTC TACTACTCAT AAAACACTTA GAGGTCCAAG AGGTGGTTTA ATTTTATCTA ACGATGAAGA GATAGGTCAC AAAATAAATT CTGCATTATT TCCAGGACTA CAAGGTGGTC CGTTAATGCA TATAATAGCT GCAAAAGCCG TAGCTTTTTT AGAAAATTTG CAGCCTGAAT ATAAAAGTTA TATTCAGCAG GTAATAAGTA ACGCTAAAGC TTTAGCAAGT AGCTTGCAAG AAAGAGGATA TGATATATTA ACAGGTGGTA CCGATAATCA TATTGTTTTA GTGGATTTGC GTAAAGACGG AATCACTGGT AAGCTTGCCG CAAATTCTTT GGATAGAGCA GGGATTACGT GTAATAAGAA TGCTATCCCG TTTGATGAAA CTTCGCCTTT TATCACTTCA GGCATTCGCC TTGGTACTCC CGCATGTACT ACTAGAGGCT TTAAAGAAAA GGATTTTGTA TTAGTCGGTC ATATGGTAGC AGATATTTTA GACGGCTTAA AAAATAATGA AGATAATAGC GCTCTTGAGC AGCAAGTATT AAATGAAGTG ACAAAATTAA TTGAATTATT TCCGTTTTAT GGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT