SSGCID: Target Page

RiprA.18411.a

VIRB10 PROTEIN (VirB10)

CENTER ID:	RiprA.18411.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.18411.a.B2.GE40089	soluble domain		58	483
RiprA.18411.a.B3.GE40090	globular domain (aln H.pyl struc)		191	483

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272947.5.peg.298
RefSeq:	WP_004597370.1
UniProt:	Q9ZDN6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVDQNNNNN TDSLSGADLP EVQRELSKVS VSFNKNIAIV VVICAILIYI FYTLFVSTKK EEIPETKIPS NIVKPVTDVD DIIPEIPKLP DPPKLERPIV SPPPPPPPPV VEVPPVLPPI AVEGNKDKTL QLPPVSLPTT SGTLVESDAE KQRREAKRKS AIVLVGGVAP KKTPEQMTEA VTFKDRGDMF LLLGRGKLID AVLETGINSD LGGEIRAIIS RDVFSEKGKV ILIPKGSKIF GKYATSTSSD SYGRVSIIWD RVDLTNGYTI EFDSPAVDNL GRPGLQGRVD NKYKEQFANA VLQSGFNIGL AKILDKLVPP PIDSQAAATN SATATQILNV AQTISSNTAM DVNTRIVTIC TNILAAITDK TSIAYATMTQ ACTTAQNASS AHTSEQRLQT LIQAVNTAAS NLLTTTSIAS TPTQAQQAST QAFTDVTNVV QNMITQQHFK PTTTVNQGTP IRIYVNKDYK FPKTVLLKSK VMK

NT Sequence

atggctgtag atcaaaataa caataataat accgattctt tatcaggggc agatttacct gaagtacaaa gagaattatc taaagtttcg gttagtttta ataagaatat tgcgatagta gtggtcattt gtgctatcct catatatatc ttttatactc tttttgttag cactaaaaaa gaagaaatac cggagactaa aataccgagt aatattgtaa agcctgttac agatgttgat gatattatcc cagagattcc aaagttaccc gatccaccga aacttgagag gccaatagtg tccccaccac cacctcctcc tccaccggta gtagaagtac ctcctgtctt acctccaatt gctgtagaag gaaataaaga taaaacactt caattacccc cggtttcgtt acctacgacc tcaggaacgt tagttgagag tgatgcagaa aaacaacgcc gtgaagcaaa aaggaaatca gctatagtgt tagttggtgg ggtagcacct aaaaaaacac cagaacaaat gacagaagca gtaacattta aagatcgtgg tgatatgttt ttgttgctcg gacgcggtaa attaattgat gcagtccttg aaacaggaat aaatagtgat ctcggtggtg aaatcagagc aattattagt agagatgtat tttctgagaa aggtaaagtt atattaatac ccaaaggatc aaaaatattc ggtaaatatg caacttcaac ttcatcggat agttacggta gggtttcgat catatgggat cgagtcgact taactaatgg ttatacaata gaatttgact cacctgcagt tgataattta ggtagacctg gattacaagg gagagttgat aataaatata aggaacaatt tgcaaatgcc gtattacaat ctggctttaa tataggtctt gctaaaatcc tagataagtt agtacctcct cctatagatt cacaggcagc ggctaccaac agcgctacag caacacaaat attaaatgta gctcaaacta tttcgtctaa cactgctatg gatgtaaata ctaggatagt tacaatttgt actaatatac ttgctgctat taccgataaa acttcgattg cttatgctac tatgactcaa gcatgtacga cagcacaaaa tgcttcttct gcacatactt ctgaacagag attgcaaaca ctaatacagg cagttaatac ggcagcttca aatttgctga ctactacatc tattgcatca actccgacac aagcacaaca agcttctaca caagctttta ctgatgtgac taatgttgta caaaatatga taactcagca acattttaag cctaccacaa cggttaatca aggtacgccg attagaatat acgttaataa ggattataaa tttccaaaaa ccgttttatt aaaatcgaag gtaatgaaat ga

Details for RiprA.18411.a.B2.GE40089

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	47kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMT KKEEIPETKI PSNIVKPVTD VDDIIPEIPK LPDPPKLERP IVSPPPPPPP PVVEVPPVLP PIAVEGNKDK TLQLPPVSLP TTSGTLVESD AEKQRREAKR KSAIVLVGGV APKKTPEQMT EAVTFKDRGD MFLLLGRGKL IDAVLETGIN SDLGGEIRAI ISRDVFSEKG KVILIPKGSK IFGKYATSTS SDSYGRVSII WDRVDLTNGY TIEFDSPAVD NLGRPGLQGR VDNKYKEQFA NAVLQSGFNI GLAKILDKLV PPPIDSQAAA TNSATATQIL NVAQTISSNT AMDVNTRIVT ICTNILAAIT DKTSIAYATM TQACTTAQNA SSAHTSEQRL QTLIQAVNTA ASNLLTTTSI ASTPTQAQQA STQAFTDVTN VVQNMITQQH FKPTTTVNQG TPIRIYVNKD YKFPKT

Validated NT Sequence

cggtttttgg aaatttataa tccttattaa cgtatattct aatcggcgta ccttgattaa ccgttgtggt aggcttaaaa tgttgctgag ttatcatatt ttgtacaaca ttagtcacat cagtaaaagc ttgtgtagaa gcttgttgtg cttgtgtcgg agttgatgca atagatgtag tagtcagcaa atttgaagct gccgtattaa ctgcctgtat tagtgtttgc aatctctgtt cagaagtatg tgcagaagaa gcattttgtg ctgtcgtaca tgcttgagtc atagtagcat aagcaatcga agttttatcg gtaatagcag caagtatatt agtacaaatt gtaactatcc tagtatttac atccatagca gtgttagacg aaatagtttg agctacattt aatatttgtg ttgctgtagc gctgttggta gccgctgcct gtgaatctat aggaggaggt actaacttat ctaggatttt agcaagacct atattaaagc cagattgtaa tacggcattt gcaaattgtt ccttatattt attatcaact ctcccttgta atccaggtct acctaaatta tcaactgcag gtgagtcaaa ttctattgta taaccattag ttaagtcgac tcgatcccat atgatcgaaa ccctaccgta actatccgat gaagttgaag ttgcatattt accgaatatt tttgatcctt tgggtattaa tataacttta cctttctcag aaaatacatc tctactaata attgctctga tttcaccacc gagatcacta tttattcctg tttcaaggac tgcatcaatt aatttaccgc gtccgagcaa caaaaacata tcaccacgat ctttaaatgt tactgcttct gtcatttgtt ctggtgtttt tttaggtgct accccaccaa ctaacactat agctgatttc ctttttgctt cacggcgttg tttttctgca tcactctcaa ctaacgttcc tgaggtcgta ggtaacgaaa ccgggggtaa ttgaagtgtt ttatctttat ttccttctac agcaattgga ggtaagacag gaggtacttc tactaccggt ggaggaggag gtggtggtgg ggacactatt ggcctctcaa gtttcggtgg atcgggtaac tttggaatct ctgggataat atcatcaaca tctgtaacag gctttacaat attactcggt attttagtct ccggtatttc ttctttttta gtcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHTK KEEIPETKIP SNIVKPVTDV DDIIPEIPKL PDPPKLERPI VSPPPPPPPP VVEVPPVLPP IAVEGNKDKT LQLPPVSLPT TSGTLVESDA EKQRREAKRK SAIVLVGGVA PKKTPEQMTE AVTFKDRGDM FLLLGRGKLI DAVLETGINS DLGGEIRAII SRDVFSEKGK VILIPKGSKI FGKYATSTSS DSYGRVSIIW DRVDLTNGYT IEFDSPAVDN LGRPGLQGRV DNKYKEQFAN AVLQSGFNIG LAKILDKLVP PPIDSQAAAT NSATATQILN VAQTISSNTA MDVNTRIVTI CTNILAAITD KTSIAYATMT QACTTAQNAS SAHTSEQRLQ TLIQAVNTAA SNLLTTTSIA STPTQAQQAS TQAFTDVTNV VQNMITQQHF KPTTTVNQGT PIRIYVNKDY KFPKTVLLKS KVMK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catactaaaa aagaagaaat accggagact aaaataccga gtaatattgt aaagcctgtt acagatgttg atgatattat cccagagatt ccaaagttac ccgatccacc gaaacttgag aggccaatag tgtccccacc accacctcct cctccaccgg tagtagaagt acctcctgtc ttacctccaa ttgctgtaga aggaaataaa gataaaacac ttcaattacc cccggtttcg ttacctacga cctcaggaac gttagttgag agtgatgcag aaaaacaacg ccgtgaagca aaaaggaaat cagctatagt gttagttggt ggggtagcac ctaaaaaaac accagaacaa atgacagaag cagtaacatt taaagatcgt ggtgatatgt ttttgttgct cggacgcggt aaattaattg atgcagtcct tgaaacagga ataaatagtg atctcggtgg tgaaatcaga gcaattatta gtagagatgt attttctgag aaaggtaaag ttatattaat acccaaagga tcaaaaatat tcggtaaata tgcaacttca acttcatcgg atagttacgg tagggtttcg atcatatggg atcgagtcga cttaactaat ggttatacaa tagaatttga ctcacctgca gttgataatt taggtagacc tggattacaa gggagagttg ataataaata taaggaacaa tttgcaaatg ccgtattaca atctggcttt aatataggtc ttgctaaaat cctagataag ttagtacctc ctcctataga ttcacaggca gcggctacca acagcgctac agcaacacaa atattaaatg tagctcaaac tatttcgtct aacactgcta tggatgtaaa tactaggata gttacaattt gtactaatat acttgctgct attaccgata aaacttcgat tgcttatgct actatgactc aagcatgtac gacagcacaa aatgcttctt ctgcacatac ttctgaacag agattgcaaa cactaataca ggcagttaat acggcagctt caaatttgct gactactaca tctattgcat caactccgac acaagcacaa caagcttcta cacaagcttt tactgatgtg actaatgttg tacaaaatat gataactcag caacatttta agcctaccac aacggttaat caaggtacgc cgattagaat atacgttaat aaggattata aatttccaaa aaccgtttta ttaaaatcga aggtaatgaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for RiprA.18411.a.B3.GE40090

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHLL LGRGKLIDAV LETGINSDLG GEIRAIISRD VFSEKGKVIL IPKGSKIFGK YATSTSSDSY GRVSIIWDRV DLTNGYTIEF DSPAVDNLGR PGLQGRVDNK YKEQFANAVL QSGFNIGLAK ILDKLVPPPI DSQAAATNSA TATQILNVAQ TISSNTAMDV NTRIVTICTN ILAAITDKTS IAYATMTQAC TTAQNASSAH TSEQRLQTLI QAVNTAASNL LTTTSIASTP TQAQQASTQA FTDVTNVVQN MITQQHFKPT TTVNQGTPIR IYVNKDYKFP KTVLLKSKVM K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgttgc tcggacgcgg taaattaatt gatgcagtcc ttgaaacagg aataaatagt gatctcggtg gtgaaatcag agcaattatt agtagagatg tattttctga gaaaggtaaa gttatattaa tacccaaagg atcaaaaata ttcggtaaat atgcaacttc aacttcatcg gatagttacg gtagggtttc gatcatatgg gatcgagtcg acttaactaa tggttataca atagaatttg actcacctgc agttgataat ttaggtagac ctggattaca agggagagtt gataataaat ataaggaaca atttgcaaat gccgtattac aatctggctt taatataggt cttgctaaaa tcctagataa gttagtacct cctcctatag attcacaggc agcggctacc aacagcgcta cagcaacaca aatattaaat gtagctcaaa ctatttcgtc taacactgct atggatgtaa atactaggat agttacaatt tgtactaata tacttgctgc tattaccgat aaaacttcga ttgcttatgc tactatgact caagcatgta cgacagcaca aaatgcttct tctgcacata cttctgaaca gagattgcaa acactaatac aggcagttaa tacggcagct tcaaatttgc tgactactac atctattgca tcaactccga cacaagcaca acaagcttct acacaagctt ttactgatgt gactaatgtt gtacaaaata tgataactca gcaacatttt aagcctacca caacggttaa tcaaggtacg ccgattagaa tatacgttaa taaggattat aaatttccaa aaaccgtttt attaaaatcg aaggtaatga aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat