RiprA.18013.a

Elongation factor G

CENTER ID:	RiprA.18013.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.18013.a.B1.GE41044	Full length(RiprA.18013.a)		1	699

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

6NOT

DEPOSITED: 1/16/2019

DETERMINATION: XRay

Diffraction Image

CLONE: RiprA.18013.a.B1.GE41044

PROTEIN: RiprA.18013.a.B1.PS38427

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272947.5.peg.134
UniProt:	P41084

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKINKLEQI RNIGICAHID AGKTTTTERI LYYTGKSHKI GEVHEGGATM DWMEQEQERG ITITSAATTC RWQDKVINII DTPGHVDFTI EVERSLRVLD GAVAVFDGVA GVEPQSETVW RQADKYNVPR MCFVNKMDRM GADFYRCVEM IKDRLGARSL IIQLPIGIEE NFKGIVNLIK MKAVIWKDES LGAEYFEEDI PADMQDKAAE YRARLLDMVV ELDDTIMEQY LSGAEITEEQ IKILIRKGTI EARFYPILCG SAFKNKGVQP LLDAIVDFLP SPIDIGIVKG IEVSTSEEKD FPISIVEPFS ALAFKIMNDP FVGSLTFIRI YSGKITSGAT VINTVKNKRE KIGRMLLMHA NNREDIKEAS AGDIVALAGL KDTSTGDTLS DIDKQVVLER MEFPEPVIEL AVEPKSTADQ EKMGLALSRL AAEDPSFRVS TDHETGQTVI KGMGELHLEI IIDRMRREFK VEANIGAPQV AYRETITTAC EIDYTHKKQS GGAGQFARVK IIFEPLKDVI DLKDEDKNKT FVFESKIVGG AVPKEYIPGV EKGLNNIRET GVIAGYPMID FKATLVDGAF HDVDSSVLAF EIAAKGAFRE GMQKGNPKLL EPIMKVEVIT PDEYMGDIIG DLNSRRGQIQ NMDPRGNAQV VTAHVPLAEM FGYVNTLRSL SQGRAQFSMI FSHYDQVPSQ VADMIKAKK

NT Sequence

ATGAGTAAAA TAAATAAACT TGAACAGATT CGTAATATCG GTATATGTGC TCACATTGAT GCTGGTAAAA CTACAACTAC CGAACGTATT TTATATTATA CTGGTAAATC ACATAAAATA GGTGAAGTTC ATGAAGGTGG TGCTACTATG GATTGGATGG AACAAGAGCA AGAACGTGGT ATAACAATTA CCTCAGCTGC TACTACTTGT AGATGGCAAG ATAAAGTAAT TAATATTATT GATACTCCAG GTCACGTTGA CTTTACAATT GAAGTAGAGC GTTCGCTGCG TGTTCTTGAC GGTGCTGTTG CAGTATTTGA TGGTGTAGCT GGGGTTGAAC CACAGTCCGA AACGGTTTGG AGGCAGGCAG ATAAATATAA TGTTCCCAGA ATGTGTTTTG TTAATAAAAT GGATAGAATG GGGGCAGATT TTTATAGATG CGTTGAAATG ATCAAAGATC GTTTAGGCGC GAGGTCACTT ATTATTCAGT TGCCTATTGG TATTGAAGAA AATTTTAAGG GGATAGTTAA TCTTATAAAA ATGAAAGCAG TGATTTGGAA AGATGAATCA CTTGGTGCTG AATATTTTGA AGAAGATATA CCCGCAGATA TGCAAGATAA AGCTGCAGAA TATCGTGCTA GGTTGCTTGA TATGGTTGTT GAGTTAGATG ACACTATCAT GGAACAATAT TTATCAGGGG CAGAAATAAC AGAAGAACAA ATTAAAATCT TAATAAGAAA AGGTACTATT GAAGCAAGAT TTTATCCAAT TTTATGTGGT AGTGCTTTTA AAAATAAAGG TGTGCAACCT TTACTTGATG CCATAGTCGA TTTCTTACCT TCGCCTATTG ATATAGGTAT AGTAAAAGGT ATAGAAGTAA GTACAAGTGA AGAAAAAGAT TTTCCTATTT CTATAGTTGA GCCTTTTTCT GCTTTAGCAT TTAAAATTAT GAATGATCCT TTTGTCGGTT CATTAACTTT TATTAGAATA TATTCAGGTA AAATTACTTC AGGTGCGACT GTTATTAATA CTGTAAAAAA TAAAAGGGAA AAAATCGGTA GAATGTTATT AATGCATGCT AATAATCGTG AAGATATAAA AGAAGCATCA GCAGGTGATA TAGTAGCTTT AGCAGGTCTT AAAGATACTA GCACTGGTGA TACATTATCC GATATTGATA AACAAGTGGT CTTAGAAAGA ATGGAATTCC CAGAGCCAGT AATTGAGCTT GCAGTAGAAC CTAAGTCAAC AGCAGATCAA GAAAAAATGG GCTTAGCACT TTCTCGTTTA GCAGCAGAAG ATCCATCATT TAGGGTTTCA ACTGATCATG AAACAGGGCA AACAGTGATA AAAGGGATGG GTGAACTTCA TTTAGAAATT ATCATTGATC GTATGAGAAG AGAGTTTAAG GTTGAAGCAA ATATTGGTGC TCCTCAAGTA GCATATCGTG AAACTATAAC AACAGCCTGC GAAATTGATT ATACTCATAA AAAACAATCT GGTGGTGCAG GACAATTTGC TCGTGTAAAA ATTATTTTTG AACCTCTTAA AGACGTAATA GATCTAAAAG ATGAAGATAA AAATAAAACT TTTGTCTTTG AAAGTAAAAT TGTTGGTGGT GCTGTTCCTA AAGAATATAT ACCAGGTGTT GAAAAAGGAT TAAATAATAT TAGAGAAACC GGTGTTATTG CCGGTTATCC TATGATTGAT TTTAAAGCGA CTTTAGTCGA TGGTGCTTTT CATGATGTAG ATTCTAGCGT ACTCGCTTTT GAAATTGCTG CAAAAGGAGC GTTTAGAGAA GGGATGCAAA AAGGTAATCC TAAGTTACTT GAGCCTATTA TGAAAGTTGA AGTAATTACT CCTGATGAAT ATATGGGTGA TATTATAGGT GACTTAAATA GTCGTAGAGG GCAAATTCAA AACATGGATC CAAGAGGAAA TGCTCAAGTA GTCACTGCGC ATGTTCCTTT AGCCGAAATG TTTGGTTATG TTAATACACT TAGATCTTTA TCTCAAGGGA GAGCGCAGTT TAGTATGATA TTCTCACATT ATGATCAAGT GCCGAGTCAA GTAGCGGATA TGATTAAAGC TAAAAAG

Details for RiprA.18013.a.B1.GE41044

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	79kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS KINKLEQIRN IGICAHIDAG KTTTTERILY YTGKSHKIGE VHEGGATMDW MEQEQERGIT ITSAATTCRW QDKVINIIDT PGHVDFTIEV ERSLRVLDGA VAVFDGVAGV EPQSETVWRQ ADKYNVPRMC FVNKMDRMGA DFYRCVEMIK DRLGARSLII QLPIGIEENF KGIVNLIKMK AVIWKDESLG AEYFEEDIPA DMQDKAAEYR ARLLDMVVEL DDTIMEQYLS GAEITEEQIK ILIRKGTIEA RFYPILCGSA FKNKGVQPLL DAIVDFLPSP IDIGIVKGIE VSTSEEKDFP ISIVEPFSAL AFKIMNDPFV GSLTFIRIYS GKITSGATVI NTVKNKREKI GRMLLMHANN REDIKEASAG DIVALAGLKD TSTGDTLSDI DKQVVLERME FPEPVIELAV EPKSTADQEK MGLALSRLAA EDPSFRVSTD HETGQTVIKG MGELHLEIII DRMRREFKVE ANIGAPQVAY RETITTACEI DYTHKKQSGG AGQFARVKII FEPLKDVIDL KDEDKNKTFV FESKIVGGAV PKEYIPGVEK GLNNIRETGV IAGYPMIDFK ATLVDGAFHD VDSSVLAFEI AAKGAFREGM QKGNPKLLEP IMKVEVITPD EYMGDIIGDL NSRRGQIQNM DPRGNAQVVT AHVPLAEMFG YVNTLRSLSQ GRAQFSMIFS HYDQVPSQVA DMIKAKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta aaataaataa acttgaacag attcgtaata tcggtatatg tgctcacatt gatgctggta aaactacaac taccgaacgt attttatatt atactggtaa atcacataaa ataggtgaag ttcatgaagg tggtgctact atggattgga tggaacaaga gcaagaacgt ggtataacaa ttacctcagc tgctactact tgtagatggc aagataaagt aattaatatt attgatactc caggtcacgt tgactttaca attgaagtag agcgttcgct gcgtgttctt gacggtgctg ttgcagtatt tgatggtgta gctggggttg aaccacagtc cgaaacggtt tggaggcagg cagataaata taatgttccc agaatgtgtt ttgttaataa aatggataga atgggggcag atttttatag atgcgttgaa atgatcaaag atcgtttagg cgcgaggtca cttattattc agttgcctat tggtattgaa gaaaatttta aggggatagt taatcttata aaaatgaaag cagtgatttg gaaagatgaa tcacttggtg ctgaatattt tgaagaagat atacccgcag atatgcaaga taaagctgca gaatatcgtg ctaggttgct tgatatggtt gttgagttag atgacactat catggaacaa tatttatcag gggcagaaat aacagaagaa caaattaaaa tcttaataag aaaaggtact attgaagcaa gattttatcc aattttatgt ggtagtgctt ttaaaaataa aggtgtgcaa cctttacttg atgccatagt cgatttctta ccttcgccta ttgatatagg tatagtaaaa ggtatagaag taagtacaag tgaagaaaaa gattttccta tttctatagt tgagcctttt tctgctttag catttaaaat tatgaatgat ccttttgtcg gttcattaac ttttattaga atatattcag gtaaaattac ttcaggtgcg actgttatta atactgtaaa aaataaaagg gaaaaaatcg gtagaatgtt attaatgcat gctaataatc gtgaagatat aaaagaagca tcagcaggtg atatagtagc tttagcaggt cttaaagata ctagcactgg tgatacatta tccgatattg ataaacaagt ggtcttagaa agaatggaat tcccagagcc agtaattgag cttgcagtag aacctaagtc aacagcagat caagaaaaaa tgggcttagc actttctcgt ttagcagcag aagatccatc atttagggtt tcaactgatc atgaaacagg gcaaacagtg ataaaaggga tgggtgaact tcatttagaa attatcattg atcgtatgag aagagagttt aaggttgaag caaatattgg tgctcctcaa gtagcatatc gtgaaactat aacaacagcc tgcgaaattg attatactca taaaaaacaa tctggtggtg caggacaatt tgctcgtgta aaaattattt ttgaacctct taaagacgta atagatctaa aagatgaaga taaaaataaa acttttgtct ttgaaagtaa aattgttggt ggtgctgttc ctaaagaata tataccaggt gttgaaaaag gattaaataa tattagagaa accggtgtta ttgccggtta tcctatgatt gattttaaag cgactttagt cgatggtgct tttcatgatg tagattctag cgtactcgct tttgaaattg ctgcaaaagg agcgtttaga gaagggatgc aaaaaggtaa tcctaagtta cttgagccta ttatgaaagt tgaagtaatt actcctgatg aatatatggg tgatattata ggtgacttaa atagtcgtag agggcaaatt caaaacatgg atccaagagg aaatgctcaa gtagtcactg cgcatgttcc tttagccgaa atgtttggtt atgttaatac acttagatct ttatctcaag ggagagcgca gtttagtatg atattctcac attatgatca agtgccgagt caagtagcgg atatgattaa agctaaaaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat