SSGCID: Target Page

RiprA.17851.a

Putative uncharacterized protein RP534

CENTER ID:	RiprA.17851.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.17851.a.B1.GE35323	Full length( RiprA.17851.a )		1	598

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272947.5.peg.544
RefSeq:	NP_220907.1
UniProt:	Q9ZD16

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDIKIRKHH IINNLKQKSK TDVFNEQSEI LEQLEYITKD QHSPINQIKT LYELEQAQKA PIEYIAFSGG GAKGAIYSGV YEAAKKTGIL DNVKAVAGSS VGAITAAVVA LGTPPKRFEE ISKNTNLQTL FGKQGFSAGM IQLNKDGKPL YDLIELIIKE NIGNFLHRSD IVNVQNDKDL DELRERYNQK DGKIYFKDIA LLRKYDPVQY KDLVITATQQ ETSALTIFNS FDTPNVEIAL ACRASASIPI VFKPVEIDGK KYVDGGYRDN IPTKYFKDNE PEFCTKEVTN NLEEITLAKK QGRTLAMVFG TGMGADANIA IYSAKNFDSP SDIVKFLVDV LFKIIAQVGG KFKYTETLRE TNEQLRENAL NTVVLDTAGI GTLDFKDAQK YCDYLHIKGY CQTLEHLNNH DLTKKIDKTF DHQKFLLNVY EVYDNKNLHK TFGTKLFEMF IPSKENNSSK WQDGIIETHN DKAKMLLSFC KTGVLTEKKL NERLKEYVII AATSRNNTLK TDTNSLNALL YTLNASAASS KIKDSFIEVL EIDKNKDTRF DKTKTFDYNI ANFQFTKKDL ESFLAKNKSA VLKIQRKHDV ARNQRSRG

NT Sequence

ATGACTGATA TAAAGATAAG AAAACATCAT ATTATTAATA ATCTGAAGCA AAAGTCCAAA ACTGATGTAT TTAATGAGCA ATCTGAAATA TTAGAGCAGT TAGAATACAT AACAAAAGAT CAACATTCTC CTATCAACCA AATCAAAACA TTGTATGAGC TAGAACAAGC ACAAAAAGCG CCTATCGAAT ATATAGCTTT TAGTGGGGGT GGAGCTAAGG GGGCAATATA TTCCGGAGTT TATGAAGCAG CAAAAAAAAC TGGTATTTTA GATAATGTTA AGGCTGTAGC CGGTTCTTCT GTTGGTGCTA TCACTGCTGC AGTGGTAGCT CTTGGGACAC CGCCTAAGCG ATTTGAAGAA ATTTCTAAAA ATACAAATCT GCAAACTTTA TTTGGAAAAC AAGGCTTTTC TGCAGGTATG ATACAGTTAA ATAAAGACGG TAAGCCTTTA TATGATTTAA TAGAACTGAT TATTAAAGAA AATATTGGGA ATTTTTTACA TAGATCAGAT ATAGTAAATG TTCAAAATGA TAAGGATCTT GATGAATTAA GAGAAAGATA TAATCAAAAG GATGGTAAAA TATATTTTAA AGATATAGCG TTATTACGAA AATACGATCC AGTGCAATAT AAGGATTTAG TAATTACAGC AACACAACAA GAGACAAGTG CATTAACTAT TTTCAATAGT TTTGATACAC CAAATGTTGA AATCGCTTTG GCTTGTCGTG CTTCTGCCTC TATACCTATT GTTTTTAAGC CGGTAGAAAT TGACGGTAAA AAATATGTTG ATGGTGGATA TAGAGATAAT ATACCGACAA AGTATTTTAA AGATAATGAA CCAGAATTTT GTACAAAAGA AGTAACTAAT AATTTGGAGG AAATTACATT AGCTAAGAAG CAAGGTAGAA CTCTGGCTAT GGTTTTTGGT ACTGGCATGG GAGCTGATGC AAATATTGCT ATATATAGTG CAAAAAATTT TGATAGTCCA AGTGATATCG TAAAGTTTTT AGTTGATGTA TTATTTAAAA TTATTGCACA AGTAGGAGGA AAATTTAAAT ATACTGAAAC TTTAAGAGAA ACTAATGAGC AATTGCGTGA AAACGCTTTA AACACCGTTG TTTTAGATAC TGCTGGAATA GGTACCTTGG ATTTTAAAGA TGCACAAAAA TATTGTGATT ATTTGCATAT TAAAGGTTAT TGTCAAACTT TAGAACATTT GAATAATCAT GACCTTACCA AAAAGATAGA TAAAACATTT GACCATCAAA AATTTCTACT AAATGTTTAT GAAGTTTATG ATAATAAAAA TTTACATAAA ACATTTGGCA CTAAATTATT TGAAATGTTT ATTCCATCAA AAGAAAATAA TTCTTCTAAA TGGCAAGACG GCATTATTGA GACTCATAAC GATAAGGCAA AAATGCTATT ATCTTTTTGT AAAACAGGAG TATTGACTGA AAAAAAATTA AATGAAAGAC TTAAAGAATA TGTAATTATT GCCGCAACTA GTCGTAACAA TACACTAAAG ACCGATACAA ACTCATTGAA TGCTTTATTA TATACATTAA ATGCTTCTGC TGCTTCAAGT AAGATAAAAG ATAGTTTCAT TGAGGTATTA GAAATAGATA AAAATAAAGA TACAAGGTTT GATAAAACTA AAACCTTTGA TTATAATATT GCTAATTTTC AATTTACAAA AAAAGATTTA GAAAGTTTTT TAGCAAAAAA TAAAAGCGCT GTGCTAAAAA TTCAACGCAA ACATGATGTA GCTAGGAATC AAAGGAGCAG GGGG

Details for RiprA.17851.a.B1.GE35323

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	74
PERCENT COVERAGE:	58

Validated AA Sequence

Validated NT Sequence

tttgtttact ttaagaagga ganataccat ggctcaccac caccaccacc atatgactga tataaagata agaaaacatc atattattaa taatctgaag caaaagtcca aaactgatgt atttaatgag caatctgaaa tattagagca gttagaatac ataacaaaag atcaacattc tcctatcaac caaatcaaaa cattgtatga gctagaacaa gcacaaaaag cgcctatcga atatatagct tttagtgggg gtggagctaa gggggcaata tattccggag tttatgaagc agcaaaaaaa actggtattt tagataatgt taaggctgta gccggttctt ctgttggtgc tatcactgct gcagtggtag ctcttgggac accgcctaag cgatttgaag aaatttctaa aaatacaaat ctgcaaactt tatttggaaa acaaggcttt tctgcaggta tgatacagtt aaataaagac ggtaagcctt tatatgattt aatagaactg attattaaag aaaatattgg gaatttttta catagatcag atatagtaaa tgttcaaaat gataaggatc ttgatgaatt aagagaaaga tataatcaaa aggatggtaa aatatatttt aaagatatag cgttattacn aaaatacgat ccngtgcaat ataaggattt agtaattacn gcaacncaac nagagacaag tgcattaact attttcaata gttttgatac nccaaatgnt gaaatcgctt tggcttgtcn ngcttntgcn tctataccta ttgnttttaa gccngtagaa attgacggta aaaatangnt gatggtggnn atngagnnaa tatacngaca antattttna anannngnac nnnnatttng nannaaanan nannannnnn tngnangant tacnttancn anannnnnan annnngnnnn nnttnnnncn gnnnggnnng atgnnannnt nctnnnnnnn nngnannant nnnnngnccn nnannnntnn nnnnnnnnnn nnnnntnncn nnnnnnnnnn tnnnnnnann tnnnnnnann nnnnnnnnnn nnnnnaa

Expected Protein Sequence

MAHHHHHHMT DIKIRKHHII NNLKQKSKTD VFNEQSEILE QLEYITKDQH SPINQIKTLY ELEQAQKAPI EYIAFSGGGA KGAIYSGVYE AAKKTGILDN VKAVAGSSVG AITAAVVALG TPPKRFEEIS KNTNLQTLFG KQGFSAGMIQ LNKDGKPLYD LIELIIKENI GNFLHRSDIV NVQNDKDLDE LRERYNQKDG KIYFKDIALL RKYDPVQYKD LVITATQQET SALTIFNSFD TPNVEIALAC RASASIPIVF KPVEIDGKKY VDGGYRDNIP TKYFKDNEPE FCTKEVTNNL EEITLAKKQG RTLAMVFGTG MGADANIAIY SAKNFDSPSD IVKFLVDVLF KIIAQVGGKF KYTETLRETN EQLRENALNT VVLDTAGIGT LDFKDAQKYC DYLHIKGYCQ TLEHLNNHDL TKKIDKTFDH QKFLLNVYEV YDNKNLHKTF GTKLFEMFIP SKENNSSKWQ DGIIETHNDK AKMLLSFCKT GVLTEKKLNE RLKEYVIIAA TSRNNTLKTD TNSLNALLYT LNASAASSKI KDSFIEVLEI DKNKDTRFDK TKTFDYNIAN FQFTKKDLES FLAKNKSAVL KIQRKHDVAR NQRSRG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg atataaagat aagaaaacat catattatta ataatctgaa gcaaaagtcc aaaactgatg tatttaatga gcaatctgaa atattagagc agttagaata cataacaaaa gatcaacatt ctcctatcaa ccaaatcaaa acattgtatg agctagaaca agcacaaaaa gcgcctatcg aatatatagc ttttagtggg ggtggagcta agggggcaat atattccgga gtttatgaag cagcaaaaaa aactggtatt ttagataatg ttaaggctgt agccggttct tctgttggtg ctatcactgc tgcagtggta gctcttggga caccgcctaa gcgatttgaa gaaatttcta aaaatacaaa tctgcaaact ttatttggaa aacaaggctt ttctgcaggt atgatacagt taaataaaga cggtaagcct ttatatgatt taatagaact gattattaaa gaaaatattg ggaatttttt acatagatca gatatagtaa atgttcaaaa tgataaggat cttgatgaat taagagaaag atataatcaa aaggatggta aaatatattt taaagatata gcgttattac gaaaatacga tccagtgcaa tataaggatt tagtaattac agcaacacaa caagagacaa gtgcattaac tattttcaat agttttgata caccaaatgt tgaaatcgct ttggcttgtc gtgcttctgc ctctatacct attgttttta agccggtaga aattgacggt aaaaaatatg ttgatggtgg atatagagat aatataccga caaagtattt taaagataat gaaccagaat tttgtacaaa agaagtaact aataatttgg aggaaattac attagctaag aagcaaggta gaactctggc tatggttttt ggtactggca tgggagctga tgcaaatatt gctatatata gtgcaaaaaa ttttgatagt ccaagtgata tcgtaaagtt tttagttgat gtattattta aaattattgc acaagtagga ggaaaattta aatatactga aactttaaga gaaactaatg agcaattgcg tgaaaacgct ttaaacaccg ttgttttaga tactgctgga ataggtacct tggattttaa agatgcacaa aaatattgtg attatttgca tattaaaggt tattgtcaaa ctttagaaca tttgaataat catgacctta ccaaaaagat agataaaaca tttgaccatc aaaaatttct actaaatgtt tatgaagttt atgataataa aaatttacat aaaacatttg gcactaaatt atttgaaatg tttattccat caaaagaaaa taattcttct aaatggcaag acggcattat tgagactcat aacgataagg caaaaatgct attatctttt tgtaaaacag gagtattgac tgaaaaaaaa ttaaatgaaa gacttaaaga atatgtaatt attgccgcaa ctagtcgtaa caatacacta aagaccgata caaactcatt gaatgcttta ttatatacat taaatgcttc tgctgcttca agtaagataa aagatagttt cattgaggta ttagaaatag ataaaaataa agatacaagg tttgataaaa ctaaaacctt tgattataat attgctaatt ttcaatttac aaaaaaagat ttagaaagtt ttttagcaaa aaataaaagc gctgtgctaa aaattcaacg caaacatgat gtagctagga atcaaaggag cagggggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at