SSGCID: Target Page

RiprA.17832.c

Uncharacterized protein RP016

CENTER ID:	RiprA.17832.c
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.17832.c.B2.GE35320	Mature protein( RiprA.17832.c )		31	302

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272947.5.peg.16
RefSeq:	NP_220411.1
UniProt:	Q9ZEC7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKLTAQNLL KKSRFLKYSL LTSISVGAVM AIPVERIAMG MDQEAFCAEL SKKLSLEFSQ SYEDTISTTQ EKNNLSNNGP SNKSDMAEEL ANVTTKSLYK VRKMQAPEFK ISENKFLNNL DSQTISKELD PNTYTTESIS QKPEIILTAS STTVSTDSNS FVTVSTASSL KPVTSYQPTP DFKPNYSLGF NTPINTNLKI VRKLSFSSEQ PQIVQHSKSM IPLMPTAPVV LPKSSIEIEP EMVSNIPRVD DTISIKSSEV REDIKGTKDQ KTLVDQFNSS IGIWSKKTGK NKYDLNKDSS QK

NT Sequence

ATGAATAAGT TAACAGCACA AAATTTACTA AAAAAATCAA GATTTTTGAA ATATTCTTTG CTTACCTCTA TTTCAGTAGG AGCTGTAATG GCAATTCCTG TTGAAAGGAT TGCTATGGGA ATGGATCAAG AAGCATTTTG TGCTGAATTA AGTAAGAAAT TATCACTCGA ATTTTCACAA TCATATGAAG ATACAATCAG TACAACACAA GAAAAAAATA ACTTATCTAA CAATGGACCA AGCAATAAAA GTGACATGGC AGAAGAGTTA GCTAATGTAA CTACCAAATC GTTGTATAAA GTTAGAAAGA TGCAAGCACC AGAATTCAAA ATCAGCGAAA ATAAGTTTCT GAATAATTTG GATTCTCAAA CAATATCAAA AGAGTTAGAT CCAAATACTT ATACAACAGA GTCAATATCT CAAAAACCAG AAATTATTCT AACAGCATCT TCGACTACTG TTAGTACAGA TTCAAATAGT TTTGTTACTG TTTCTACTGC TTCATCATTA AAACCTGTAA CTTCGTATCA GCCCACTCCA GATTTTAAAC CAAATTATAG TTTAGGATTT AATACACCAA TAAATACAAA TTTAAAGATA GTAAGAAAAC TATCATTTAG TAGTGAGCAG CCGCAGATAG TACAGCATAG TAAATCAATG ATCCCTTTGA TGCCAACAGC ACCTGTAGTA TTGCCTAAAA GTAGTATTGA AATTGAGCCA GAAATGGTAA GTAATATACC ACGAGTTGAT GATACGATAA GCATCAAATC ATCTGAAGTA AGAGAGGATA TTAAAGGTAC GAAAGATCAA AAAACACTAG TAGATCAATT CAATAGTAGC ATAGGTATCT GGAGCAAAAA GACAGGAAAA AATAAGTATG ATTTGAATAA AGATAGTTCT CAGAAA

Details for RiprA.17832.c.B2.GE35320

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	31kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA IPVERIAMGM DQEAFCAELS KKLSLEFSQS YEDTISTTQE KNNLSNNGPS NKSDMAEELA NVTTKSLYKV RKMQAPEFKI SENKFLNNLD SQTISKELDP NTYTTESISQ KPEIILTASS TTVSTDSNSF VTVSTASSLK PVTSYQPTPD FKPNYSLGFN TPINTNLKIV RKLSFSSEQP QIVQHSKSMI PLMPTAPVVL PKSSIEIEPE MVSNIPRVDD TISIKSSEVR EDIKGTKDQK TLVDQFNSSI GIWSKKTGKN KYDXNKDSSQ K

Validated NT Sequence

atcttactca cttatttctg agaactatct ttattcnaat catacttatt ttttcctgtc tttttgctcc agatacctat gctactattg aattgatcta ctagtgtttt ttgatctttc gtacctttaa tatcctctct tacttcagat gatttgatgc ttatcgtatc atcaactcgt ggtatattac ttaccatttc tggctcaatt tcaatactac ttttaggcaa tactacaggt gctgttggca tcaaagggat cattgattta ctatgctgta ctatctgcgg ctgctcacta ctaaatgata gttttcttac tatctttaaa tttgtattta ttggtgtatt aaatcctaaa ctataatttg gtttaaaatc tggagtgggc tgatacgaag ttacaggttt taatgatgaa gcagtagaaa cagtaacaaa actatttgaa tctgtactaa cagtagtcga agatgctgtt agaataattt ctggtttttg agatattgac tctgttgtat aagtatttgg atctaactct tttgatattg tttgagaatc caaattattc agaaacttat tttcgctgat tttgaattct ggtgcttgca tctttctaac tttatacaac gatttggtag ttacattagc taactcttct gccatgtcac ttttattgct tggtccattg ttagataagt tatttttttc ttgtgttgta ctgattgtat cttcatatga ttgtgaaaat tcgagtgata atttcttact taattcagca caaaatgctt cttgatccat tcccatagca atcctttcaa caggaattgc catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa ttcctgttga aaggattgct atgggaatgg atcaagaagc attttgtgct gaattaagta agaaattatc actcgaattt tcacaatcat atgaagatac aatcagtaca acacaagaaa aaaataactt atctaacaat ggaccaagca ataaaagtga catggcagaa gagttagcta atgtaactac caaatcgttg tataaagtta gaaagatgca agcaccagaa ttcaaaatca gcgaaaataa gtttctgaat aatttggatt ctcaaacaat atcaaaagag ttagatccaa atacttatac aacagagtca atatctcaaa aaccagaaat tattctaaca gcatcttcga ctactgttag tacagattca aatagttttg ttactgtttc tactgcttca tcattaaaac ctgtaacttc gtatcagccc actccagatt ttaaaccaaa ttatagttta ggatttaata caccaataaa tacaaattta aagatagtaa gaaaactatc atttagtagt gagcagccgc agatagtaca gcatagtaaa tcaatgatcc ctttgatgcc aacagcacct gtagtattgc ctaaaagtag tattgaaatt gagccagaaa tggtaagtaa tataccacga gttgatgata cgataagcat caaatcatct gaagtaagag aggatattaa aggtacgaaa gatcaaaaaa cactagtaga tcaattcaat agtagcatag gtatctggag caaaaagaca ggaaaaaata agtatgattt gaataaagat agttctcaga aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat