RiprA.17832.a

190 KD ANTIGEN (Sca2)

CENTER ID:	RiprA.17832.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.17832.a.B1.GE35318	Full length( RiprA.17832.a )		1	341

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130526
PATRIC ID:	fig\|272947.5.peg.80
RefSeq:	NP_220474.1
UniProt:	Q9ZE66

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDVSFKTETI TEQDEATQRQ QVSDDTSRKV AILVKATSTL HKPVHHNILS DRLKVTVIGA GDEKTNVNRG IWISGLYGVN KQGVWKNIPK YQGRTTGLTI GADAEFINNH DVIGIAYSNL ESKIKYNKKL GKTAVHGHLL SVYGLKELVK GFSLQSITSY GHNYIKNKSK NLNKIIGKYQ NNNFQTLLNY KYRTKYNLHF IPSIGFKYDY SRASNYKEYN VDIENLMIQK KSNQSFESSI GAKIVSKPII SKNNIILTLS AHGNIERHFN NKNTKVNAKA TFKKQTLQET IIIPKQPKLG YNIGNNILMS IKNINVLLEY NYYTHKKYHS HQGLVKLKVN L

NT Sequence

ATGGATGTGT CATTCAAAAC TGAAACAATA ACAGAACAAG ATGAAGCGAC ACAGAGACAA CAAGTATCTG ATGACACTAG TAGAAAAGTA GCAATCTTAG TTAAGGCAAC TTCAACTCTT CACAAACCTG TTCATCACAA TATTTTAAGT GATAGATTAA AGGTGACTGT CATAGGAGCA GGTGATGAAA AAACTAATGT AAACAGGGGG ATATGGATTA GCGGCTTATA CGGGGTTAAC AAACAAGGAG TTTGGAAGAA CATTCCAAAA TATCAAGGTC GTACAACCGG TCTCACTATC GGGGCTGATG CTGAATTCAT CAATAATCAT GATGTTATTG GAATCGCTTA TAGTAATCTA GAATCAAAAA TTAAATATAA TAAAAAACTA GGGAAAACAG CAGTGCATGG TCATCTTTTA AGTGTTTATG GTCTAAAAGA ACTAGTTAAA GGTTTCTCAT TGCAGTCTAT AACATCTTAT GGTCATAATT ATATTAAGAA TAAATCTAAA AATTTAAATA AAATAATTGG AAAATATCAA AATAATAATT TTCAAACTTT ATTGAATTAT AAATATCGTA CAAAATACAA TTTACATTTT ATTCCTAGTA TAGGTTTTAA ATATGATTAT TCAAGAGCTA GCAACTATAA AGAATATAAC GTAGATATAG AAAATCTTAT GATTCAAAAG AAATCAAATC AATCATTTGA AAGTAGCATT GGCGCTAAAA TAGTTTCTAA GCCTATAATA AGCAAAAATA ATATAATATT AACACTAAGT GCTCATGGTA ATATTGAACG TCACTTCAAT AATAAAAATA CAAAAGTTAA TGCAAAAGCA ACTTTTAAAA AACAAACATT ACAAGAAACT ATCATTATAC CGAAACAACC TAAACTTGGA TATAACATCG GTAATAATAT ACTTATGAGT ATAAAAAATA TCAATGTGCT ACTTGAGTAT AATTATTATA CTCATAAAAA ATATCACAGC CATCAAGGAC TTGTTAAGTT AAAAGTTAAT TTG

Details for RiprA.17832.a.B1.GE35318

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMD VSFKTETITE QDEATQRQQV SDDTSRKVAI LVKATSTLHK PVHHNILSDR LKVTVIGAGD EKTNVNRGIW ISGLYGVNKQ GVWKNIPKYQ GRTTGLTIGA DAEFINNHDV IGIAYSNLES KIKYNKKLGK TAVHGHLLSV YGLKELVKGF SLQSITSYGH NYIKNKSKNL NKIIGKYQNN NFQTLLNYKY RTKYNLHFIP SIGFKYDYSR ASNYKEYNVD IENLMIQKKS NQSFESSIGA KIVSKPIISK NNIILTLSAH GNIERHFNNK NTKVNAKATF KKQTLQETII IPKQPKLGYN IGNNILMSIK NINVLLEYNY YTHKKYHSHQ GLVKLKVNL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatg tgtcattcaa aactgaaaca ataacagaac aagatgaagc gacacagaga caacaagtat ctgatgacac tagtagaaaa gtagcaatct tagttaaggc aacttcaact cttcacaaac ctgttcatca caatatttta agtgatagat taaaggtgac tgtcatagga gcaggtgatg aaaaaactaa tgtaaacagg gggatatgga ttagcggctt atacggggtt aacaaacaag gagtttggaa gaacattcca aaatatcaag gtcgtacaac cggtctcact atcggggctg atgctgaatt catcaataat catgatgtta ttggaatcgc ttatagtaat ctagaatcaa aaattaaata taataaaaaa ctagggaaaa cagcagtgca tggtcatctt ttaagtgttt atggtctaaa agaactagtt aaaggtttct cattgcagtc tataacatct tatggtcata attatattaa gaataaatct aaaaatttaa ataaaataat tggaaaatat caaaataata attttcaaac tttattgaat tataaatatc gtacaaaata caatttacat tttattccta gtataggttt taaatatgat tattcaagag ctagcaacta taaagaatat aacgtagata tagaaaatct tatgattcaa aagaaatcaa atcaatcatt tgaaagtagc attggcgcta aaatagtttc taagcctata ataagcaaaa ataatataat attaacacta agtgctcatg gtaatattga acgtcacttc aataataaaa atacaaaagt taatgcaaaa gcaactttta aaaaacaaac attacaagaa actatcatta taccgaaaca acctaaactt ggatataaca tcggtaataa tatacttatg agtataaaaa atatcaatgt gctacttgag tataattatt atactcataa aaaatatcac agccatcaag gacttgttaa gttaaaagtt aatttgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t