SSGCID: Target Page

RiprA.17669.a

Putative uncharacterized protein RP332

CENTER ID:	RiprA.17669.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.17669.a.A2.GE31117	Mature protein( RiprA.17669.a )		21	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272947.5.peg.342
RefSeq:	NP_220715.1
UniProt:	Q9ZDJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKILILGVTG MLGNSMFRFL TQDSKFNVCA TARSNAASLY FSKDLTNKLI TNVDVENHDS LVEVLNKTNP DVVINCIGLV KQLADVNDPL KALPINSLLP HRLANLCGLI GSRLIHISTD CVFSGKRGNY KESDFPDCND LYGRSKFLGE VDYPHAITLR TSIIGHELAS NRSLINWFLS TEGYVKGFTK AIYSGFPTVE FARIIRDFVL PNTELHGLYH VASHPINKFD LLKLVAEIYN KEIEIIPSDE LIVERSLDST RFNSITGYKP PEWRELVKRM YEFR

NT Sequence

ATGAAAATAT TAATTTTAGG TGTTACAGGT ATGCTGGGTA ACAGCATGTT TAGATTTTTA ACTCAGGATA GTAAATTTAA CGTTTGTGCA ACCGCTCGCA GTAATGCAGC AAGTTTATAC TTTTCTAAGG ATTTAACAAA TAAATTAATT ACAAATGTAG ATGTTGAAAA CCATGATTCT TTAGTTGAGG TTTTAAATAA AACAAATCCT GATGTTGTCA TAAATTGTAT AGGGCTTGTA AAGCAATTAG CTGATGTAAA TGATCCATTA AAAGCATTAC CAATAAATAG TTTATTGCCT CATAGGTTAG CAAATTTATG CGGACTTATA GGTAGTAGAT TAATCCATAT TAGTACGGAT TGTGTATTTT CAGGAAAAAG AGGTAATTAT AAAGAAAGTG ATTTTCCTGA TTGTAATGAT CTGTATGGTC GCTCAAAATT TCTCGGTGAA GTAGATTATC CGCATGCTAT CACTTTACGT ACTTCTATTA TAGGTCATGA GTTAGCAAGC AATAGAAGTT TAATAAACTG GTTTTTAAGT ACAGAAGGTT ATGTCAAAGG TTTTACAAAA GCTATTTATT CAGGATTCCC GACGGTAGAG TTTGCAAGAA TAATAAGAGA TTTTGTATTA CCAAATACAG AACTACATGG ACTTTATCAT GTGGCATCAC ACCCTATTAA TAAGTTCGAT TTGTTAAAAT TAGTCGCAGA AATATATAAT AAAGAAATTG AAATTATTCC TTCAGATGAG CTGATAGTAG AACGCTCACT AGATTCTACT CGTTTTAACT CTATAACTGG ATATAAACCT CCTGAATGGC GTGAACTTGT AAAGCGCATG TATGAGTTTA GG

Details for RiprA.17669.a.A2.GE31117

HARVESTED ON:	12/21/2010
SEQUENCED ON:	12/27/2010
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	81

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTQDSKFNV CATARSNAAS LYFSKDLTNK LITNVDVENH DSLVEVLNKT NPDVVINCIG LVKQLADVND PLKALPINSL LPHRLANLCG LIGSRLIHIS TDCVFSGKRG NYKESDFPDC NDLYGRSKFL GEVDYPHAIT LRTSIIGHEL ASNRSLINWF LSTEGYVKGF TKAIYSGFPT VEFARIIRDF VLPNTELHGL YHVASHPINX FDC

Validated NT Sequence

annannnnnn nnnnnnntac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgactca ggatagtaaa tttaacgttt gtgcaaccgc tcgcagtaat gcagcaagtt tatacttttc taaggattta acaaataaat taattacaaa tgtagatgtt gaaaaccatg attctttagt tgaggtttta aataaaacaa atcctgatgt tgtcataaat tgtatagggc ttgtaaagca attagctgat gtaaatgatc cattaaaagc attaccaata aatagtttat tgcctcatag gttagcaaat ttatgcggac ttataggtag tagattaatc catattagta cggattgtgt attttcagga aaaagaggta attataaaga aagtgatttt cctgattgta atgatctgta tggtcgctca aaatttctcg gtgaagtaga ttatccgcat gctatcactt tacgtacttc tattataggt catgagttag caagcaatag aagtttaata aactggtttt taagtacaga aggttatgtc aaaggtttta caaaagctat ttattcagga ttcccgacgg tagagtttgc nagaataata agagattttg tattaccaaa tacagaacta catggacttt atcatgtggc atcacaccct attaatnagt tcgattgtta aaantagtcg cagaaatata taataaagaa attgaaatta ttccttnnga tgagctgata gtanaangct cactagattc tactcgtttt aactctataa ctggatataa acctcctgna tggcgtgnnn ttgtaaagcg cangnntgag tttangtnan aancagcacg aacnngttct gcagccaann tntcgaggnn ccngctgcna acaaannnnn aaannaagct ganntngnnn nnnnnnnnnn nnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactcaggat agtaaattta acgtttgtgc aaccgctcgc agtaatgcag caagtttata cttttctaag gatttaacaa ataaattaat tacaaatgta gatgttgaaa accatgattc tttagttgag gttttaaata aaacaaatcc tgatgttgtc ataaattgta tagggcttgt aaagcaatta gctgatgtaa atgatccatt aaaagcatta ccaataaata gtttattgcc tcataggtta gcaaatttat gcggacttat aggtagtaga ttaatccata ttagtacgga ttgtgtattt tcaggaaaaa gaggtaatta taaagaaagt gattttcctg attgtaatga tctgtatggt cgctcaaaat ttctcggtga agtagattat ccgcatgcta tcactttacg tacttctatt ataggtcatg agttagcaag caatagaagt ttaataaact ggtttttaag tacagaaggt tatgtcaaag gttttacaaa agctatttat tcaggattcc cgacggtaga gtttgcaaga ataataagag attttgtatt accaaataca gaactacatg gactttatca tgtggcatca caccctatta ataagttcga tttgttaaaa ttagtcgcag aaatatataa taaagaaatt gaaattattc cttcagatga gctgatagta gaacgctcac tagattctac tcgttttaac tctataactg gatataaacc tcctgaatgg cgtgaacttg taaagcgcat gtatgagttt aggtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa