SSGCID: Target Page

RiprA.17583.b

PROTEASE II (PtrB)

CENTER ID:	RiprA.17583.b
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.17583.b.B1.GE35317	Full length( RiprA.17583.b )		1	684

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272947.5.peg.286
RefSeq:	NP_220665.1
UniProt:	Q9ZDP6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPPIANKQN YSFEIHKQTI KDDYNWLRDP NWPNVEEDKI LDYLKAENKY TENFFLNLKK DKKKIFEELK GRIKLDDESV YIKKKDYYYY QRVERDKNYP IYCRKHNSIE ATEEVILDVN LLASKGSFTD IGEIAMSPDQ NLMAYSVNFT GNEQYNIKIY NIKKQQYLSN EVKDVAPVII WHARLNGFFY ITINENQRWD RVIFHSLDRN ITQDKLIFEV KNPIHFISCK RSASHEYIFI NSGDHNENEI YVISMQDDSF TPKLVRVAEN KVFYEVEHNG DFFYIKTNYK AKNFHIVKVP INNFENANWN DIYIQEEQDK YLKSFDVTKN YLILNYRYNG LPLIKIKQFQ DQHENIIHFP DVSFQANSFS TNFEEDNIRI DYSSLARPNT TYSYDFDKNK LTILKDQEIP SGFNPEEYKV ERIFADNEGV KVPITLFYKK SLFKKDGSNP LYLIGYGAYG ITMPVNFRNI AVTLTNRGFI YAIAHIRGGD DLGHDWYEAA KFLTKKRTFE DFIACSRTLI KEKYTNNNNI VIAGGSAGGM LIGYVLNEKP EIYRAAIAHV PFVDVLNTML DENLPLTLLE YNEWGNPKEQ EYFDYIKSYS PYDNVKTQNY PALFIRCGIS DPRVGYWEPA KWVAKLRELK TDNNPLLLKI NMDTGHQGAA GRFDYLQEIT DELVFIFKIF DVEI

NT Sequence

ATGAAACCAC CAATCGCAAA TAAACAAAAT TATAGTTTTG AAATCCACAA GCAAACAATA AAAGATGATT ATAACTGGCT GCGTGATCCT AATTGGCCAA ATGTAGAAGA AGATAAAATT TTAGATTACT TAAAAGCCGA GAATAAATAT ACTGAAAATT TTTTTCTCAA TCTGAAAAAA GATAAAAAAA AGATTTTTGA AGAGTTAAAA GGACGTATTA AATTAGATGA TGAATCAGTT TATATTAAGA AAAAAGATTA TTACTATTAT CAGAGAGTAG AACGAGATAA AAATTATCCT ATATATTGTC GAAAGCATAA TAGTATCGAG GCAACTGAAG AAGTAATATT AGATGTAAAC TTACTTGCTT CAAAAGGGAG TTTTACCGAT ATTGGAGAAA TTGCAATGTC ACCTGATCAA AATTTAATGG CTTATAGCGT TAATTTTACA GGTAATGAGC AATATAATAT CAAAATATAC AATATTAAAA AACAACAATA CTTATCTAAT GAAGTAAAGG ATGTCGCTCC TGTTATAATT TGGCATGCGC GACTTAACGG CTTTTTCTAT ATTACTATTA ATGAAAATCA ACGCTGGGAT AGGGTCATAT TTCATAGTTT AGACAGAAAT ATTACTCAAG ACAAATTAAT ATTTGAGGTA AAAAACCCTA TCCATTTTAT TAGCTGCAAA AGATCCGCAA GTCATGAATA TATTTTTATA AATTCAGGTG ATCATAATGA AAACGAAATT TATGTAATTT CTATGCAGGA TGATAGTTTT ACACCTAAAT TAGTACGTGT AGCAGAAAAT AAAGTATTCT ATGAAGTAGA ACATAATGGT GATTTTTTTT ATATTAAAAC TAATTATAAA GCTAAAAATT TTCATATTGT AAAAGTTCCA ATTAATAATT TTGAAAATGC TAATTGGAAT GATATTTATA TTCAAGAAGA ACAGGACAAA TATTTAAAAA GCTTTGATGT TACAAAAAAT TATCTAATTT TAAATTATCG TTATAATGGA TTGCCACTTA TTAAAATAAA ACAATTTCAA GATCAACATG AAAACATAAT TCATTTTCCC GATGTAAGTT TCCAAGCAAA TAGTTTTTCT ACTAACTTTG AAGAAGATAA CATCAGAATA GATTATTCTT CTCTAGCAAG ACCAAATACC ACTTATAGCT ACGATTTTGA TAAAAATAAA TTAACAATAT TAAAGGACCA AGAAATACCT TCTGGCTTTA ATCCAGAAGA GTATAAAGTA GAAAGAATAT TTGCAGATAA TGAAGGTGTA AAAGTACCGA TAACATTATT TTATAAAAAA TCTTTATTTA AAAAAGATGG TTCGAATCCT TTATATCTAA TAGGTTATGG AGCTTATGGT ATCACTATGC CGGTTAATTT TAGAAACATA GCAGTAACGC TTACAAATCG TGGTTTTATT TATGCTATTG CTCATATTAG AGGTGGTGAT GATTTAGGAC ATGATTGGTA TGAAGCTGCT AAATTTTTAA CAAAAAAAAG GACTTTTGAA GATTTTATCG CATGTAGTAG AACTTTAATT AAAGAAAAAT ATACAAATAA TAATAATATA GTAATAGCGG GCGGAAGTGC TGGCGGCATG TTAATCGGTT ATGTTCTAAA CGAAAAACCA GAAATTTATA GAGCAGCGAT AGCTCATGTA CCATTTGTAG ATGTATTAAA TACTATGCTT GATGAAAACT TACCACTCAC TCTCTTAGAA TATAATGAAT GGGGTAATCC AAAAGAGCAA GAATATTTTG ATTATATTAA ATCATATTCG CCTTATGATA ATGTCAAAAC ACAAAACTAC CCTGCTCTAT TCATTAGGTG TGGCATATCT GACCCGCGCG TTGGTTATTG GGAACCAGCT AAATGGGTAG CAAAATTACG AGAATTAAAA ACTGATAATA ATCCTTTATT ATTAAAAATA AATATGGATA CGGGACATCA AGGTGCTGCT GGTCGTTTTG ATTATTTGCA AGAGATAACT GACGAATTAG TATTTATTTT TAAAATCTTT GATGTAGAGA TT

Details for RiprA.17583.b.B1.GE35317

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	81kDa
OBSERVED MW:	81kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	43

Validated AA Sequence

Validated NT Sequence

ttgnntannt taagaaggag anataccatg gctcaccacc accaccacca tatgaaacca ccaatcgcaa ataaacaaaa ttatagtttt gaaatccaca agcaaacaat aaaagatgat tataactggc tgcgtgatcc taattggcca aatgtagaag aagataaaat tttagattac ttaaaagccg agaataaata tactgaaaat ttttttctca atctgaaaaa agataaaaaa aagatttttg aagagttaaa aggacgtatt aaattagatg atgaatcagt ttatattaag aaaaaagatt attactatta tcagagagta gaacgagata aaaattatcc tatatattgt cgaaagcata atagtatcga ggcaactgaa gaagtaatat tagatgtaaa cttacttgct tcaaaaggga gttttaccga tattggagaa attgcaatgt cacctgatca aaatttaatg gcttatagcg ttaattttac aggtaatgag caatataata tcaaaatata caatattaaa aaacaacaat acttatctaa tgaagtaaag gatgtcgctc ctgttataat ttggcatgcg cgacttaacg gctttttcta tattactatt aatgaaaatc aacgctggga tagggtcata tttcatagtt tagacagaaa tattactcaa gacaaattaa tatttgaggt aaaaaaccct atccatttta ttagctgcna aagatccgca agtcatgaat atatttttat aaattcaggt gatcataatg aaaacgaaat ttatgtaatt tctatgcagg atganagttt tacncctaaa ttagtacgtg tagcanaaaa taaagtattc tatgaagtag aacataatgg ngattttttt tatattaaaa ctaattataa agctaaaatt ttnntattgt aaaagtnccn attaataatt ttgaaaatgc taattggnat gatnnttata ntcananana ggacnaatat ttnaaagctt tgangtncna aaaatnnnct antttnantn atcntnnnan nnnnngccnn taanaannnn tncanatcnn nnnnannnnn nnnnttnccn annnnnnnnn ancannnnnt tnnnnnnnnc ttnnnnaann a

Expected Protein Sequence

MAHHHHHHMK PPIANKQNYS FEIHKQTIKD DYNWLRDPNW PNVEEDKILD YLKAENKYTE NFFLNLKKDK KKIFEELKGR IKLDDESVYI KKKDYYYYQR VERDKNYPIY CRKHNSIEAT EEVILDVNLL ASKGSFTDIG EIAMSPDQNL MAYSVNFTGN EQYNIKIYNI KKQQYLSNEV KDVAPVIIWH ARLNGFFYIT INENQRWDRV IFHSLDRNIT QDKLIFEVKN PIHFISCKRS ASHEYIFINS GDHNENEIYV ISMQDDSFTP KLVRVAENKV FYEVEHNGDF FYIKTNYKAK NFHIVKVPIN NFENANWNDI YIQEEQDKYL KSFDVTKNYL ILNYRYNGLP LIKIKQFQDQ HENIIHFPDV SFQANSFSTN FEEDNIRIDY SSLARPNTTY SYDFDKNKLT ILKDQEIPSG FNPEEYKVER IFADNEGVKV PITLFYKKSL FKKDGSNPLY LIGYGAYGIT MPVNFRNIAV TLTNRGFIYA IAHIRGGDDL GHDWYEAAKF LTKKRTFEDF IACSRTLIKE KYTNNNNIVI AGGSAGGMLI GYVLNEKPEI YRAAIAHVPF VDVLNTMLDE NLPLTLLEYN EWGNPKEQEY FDYIKSYSPY DNVKTQNYPA LFIRCGISDP RVGYWEPAKW VAKLRELKTD NNPLLLKINM DTGHQGAAGR FDYLQEITDE LVFIFKIFDV EI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac caccaatcgc aaataaacaa aattatagtt ttgaaatcca caagcaaaca ataaaagatg attataactg gctgcgtgat cctaattggc caaatgtaga agaagataaa attttagatt acttaaaagc cgagaataaa tatactgaaa atttttttct caatctgaaa aaagataaaa aaaagatttt tgaagagtta aaaggacgta ttaaattaga tgatgaatca gtttatatta agaaaaaaga ttattactat tatcagagag tagaacgaga taaaaattat cctatatatt gtcgaaagca taatagtatc gaggcaactg aagaagtaat attagatgta aacttacttg cttcaaaagg gagttttacc gatattggag aaattgcaat gtcacctgat caaaatttaa tggcttatag cgttaatttt acaggtaatg agcaatataa tatcaaaata tacaatatta aaaaacaaca atacttatct aatgaagtaa aggatgtcgc tcctgttata atttggcatg cgcgacttaa cggctttttc tatattacta ttaatgaaaa tcaacgctgg gatagggtca tatttcatag tttagacaga aatattactc aagacaaatt aatatttgag gtaaaaaacc ctatccattt tattagctgc aaaagatccg caagtcatga atatattttt ataaattcag gtgatcataa tgaaaacgaa atttatgtaa tttctatgca ggatgatagt tttacaccta aattagtacg tgtagcagaa aataaagtat tctatgaagt agaacataat ggtgattttt tttatattaa aactaattat aaagctaaaa attttcatat tgtaaaagtt ccaattaata attttgaaaa tgctaattgg aatgatattt atattcaaga agaacaggac aaatatttaa aaagctttga tgttacaaaa aattatctaa ttttaaatta tcgttataat ggattgccac ttattaaaat aaaacaattt caagatcaac atgaaaacat aattcatttt cccgatgtaa gtttccaagc aaatagtttt tctactaact ttgaagaaga taacatcaga atagattatt cttctctagc aagaccaaat accacttata gctacgattt tgataaaaat aaattaacaa tattaaagga ccaagaaata ccttctggct ttaatccaga agagtataaa gtagaaagaa tatttgcaga taatgaaggt gtaaaagtac cgataacatt attttataaa aaatctttat ttaaaaaaga tggttcgaat cctttatatc taataggtta tggagcttat ggtatcacta tgccggttaa ttttagaaac atagcagtaa cgcttacaaa tcgtggtttt atttatgcta ttgctcatat tagaggtggt gatgatttag gacatgattg gtatgaagct gctaaatttt taacaaaaaa aaggactttt gaagatttta tcgcatgtag tagaacttta attaaagaaa aatatacaaa taataataat atagtaatag cgggcggaag tgctggcggc atgttaatcg gttatgttct aaacgaaaaa ccagaaattt atagagcagc gatagctcat gtaccatttg tagatgtatt aaatactatg cttgatgaaa acttaccact cactctctta gaatataatg aatggggtaa tccaaaagag caagaatatt ttgattatat taaatcatat tcgccttatg ataatgtcaa aacacaaaac taccctgctc tattcattag gtgtggcata tctgacccgc gcgttggtta ttgggaacca gctaaatggg tagcaaaatt acgagaatta aaaactgata ataatccttt attattaaaa ataaatatgg atacgggaca tcaaggtgct gctggtcgtt ttgattattt gcaagagata actgacgaat tagtatttat ttttaaaatc tttgatgtag agatttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat