RiprA.17300.a

OUTER MEMBRANE PROTEIN TOLC (TolC)

CENTER ID: RiprA.17300.a
ORGANISM: Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE: Endemic typhus
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiprA.17300.a.B2.GE35557 Mature protein( RiprA.17300.a ) 21 456
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272947.5.peg.232
RefSeq: NP_220610.1
UniProt: Q9ZDV0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKFTITIFI TTLLFTGSVI ALDLEQALTE GYKNNEELKA AQIKFLNAIE QFPQAFSGFM PNVGLQINRQ NSKTKYNKKY VNRLGITPRE TASTQGILTI EQSLFNGGAS IAALKAAQSG FRASRSEYYA GEQKVLLNLI TAYLDCVESK EKYDISESRV RTNIQQVKTV EEKLRLGEAT AIDIAAARAG LAAAETNKLA AYADFQGKKA NFIKVFGIEA NDITMPDLPD RLPISLDEFT RKAAKFNPDI NSARHNVTVT KALEMVQKGK LLPQVSVKLL SGGTNYNPQE PVIQNINNRI YTTTLSVNIP IYPEGGAQYS RIRSAKNQTR NSVVQLDSAI KQIKAGVVSV WEGFETAKSR IVAANQGVEA AQISYNGIVQ EEIVGSKTIL DVLDAEQKLY EAKITRVDAY KNSVLASYQM KLLTGELTAK SLKLKVKYFS PEEEFNNLKK KMFIGF
NT Sequence
ATGAGTAAAT TCACTATCAC TATATTTATT ACTACATTAT TGTTTACTGG TTCCGTTATC GCTCTTGATT TGGAGCAAGC TTTAACTGAA GGATATAAGA ATAATGAGGA ACTGAAAGCT GCTCAAATTA AGTTTTTGAA TGCAATTGAA CAGTTTCCTC AAGCTTTCTC AGGGTTTATG CCTAATGTAG GTTTACAGAT TAATAGACAA AATAGTAAGA CTAAATATAA TAAAAAATAT GTTAATAGGC TTGGTATTAC TCCACGTGAG ACAGCTAGCA CTCAAGGGAT ATTAACAATT GAACAATCAT TATTTAATGG CGGTGCTAGT ATTGCTGCTC TTAAAGCTGC TCAATCAGGA TTTAGAGCGT CACGATCCGA ATACTATGCT GGTGAGCAAA AAGTATTATT AAATTTAATA ACTGCTTATC TTGATTGTGT TGAGAGTAAG GAGAAATATG ATATCTCTGA AAGTAGGGTA CGTACTAATA TACAGCAAGT TAAGACTGTT GAAGAGAAAT TAAGACTAGG TGAAGCAACA GCAATAGATA TAGCTGCTGC AAGAGCAGGA CTTGCAGCAG CAGAAACAAA TAAACTAGCT GCTTATGCAG ATTTTCAAGG GAAAAAAGCA AATTTTATTA AAGTGTTTGG AATAGAGGCA AATGATATAA CTATGCCTGA TTTACCTGAT AGGTTACCTA TTTCGTTAGA TGAGTTCACA AGAAAAGCTG CGAAGTTCAA TCCTGATATT AATTCAGCAA GGCATAACGT AACTGTTACT AAAGCTTTAG AAATGGTGCA AAAAGGGAAA TTATTGCCGC AAGTAAGTGT AAAGTTGCTA TCAGGGGGGA CTAATTATAA TCCACAAGAG CCAGTTATTC AAAATATAAA CAATAGGATC TATACTACTA CTCTTTCGGT AAATATCCCT ATTTATCCTG AAGGAGGAGC GCAATATTCA AGAATAAGAT CAGCTAAAAA TCAAACAAGG AATAGTGTAG TCCAGCTTGA TAGTGCCATA AAGCAGATAA AAGCAGGTGT TGTTAGTGTG TGGGAAGGAT TTGAAACAGC AAAATCTCGT ATTGTTGCAG CTAATCAAGG AGTAGAAGCT GCACAAATAT CATATAACGG TATTGTGCAA GAGGAAATAG TTGGTTCTAA AACTATACTT GATGTTTTAG ATGCTGAACA AAAATTGTAT GAAGCAAAAA TAACGCGTGT TGATGCTTAT AAGAATTCAG TACTTGCTTC ATATCAAATG AAATTGTTAA CTGGTGAGCT AACTGCTAAA AGTTTAAAAC TTAAAGTAAA ATATTTTAGT CCTGAAGAAG AGTTTAACAA TCTTAAAAAG AAAATGTTTA TAGGTTTC
Details for RiprA.17300.a.B2.GE35557
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 86
Validated AA Sequence
MPNVGLQINR QNSKTKYNKK YVNRLGITPR ETASTQGILT IEQSLFNGGA SIAALKAAQS GFRASRSEYY AGEQKVLLNL ITAYLDCVES KEKYDISESR VRTNIQQVKT VEEKLRLGEA TAIDIAAARA GLAAAETNKL AAYADFQGKK ANFIKVFGIE ANDITMPDLP DRLPISLDEF TRKAAKFNPD INSARHNVTV TKALEMVQKG KLLPQVSVKL LSGGTNYNPQ EPVIQNINNR IYTTTLSVNI PIYPEGGAQY SRIRSAKNQT RNSVVQLDSA IKQIKAGVVS VWEGFETAKS RIVAANQGVE AAQISYNGIV QEEIVGSKTI LDVLDAEQKL YEAKITRVDA YKNSVLASYQ MKLLTGELTA KSLKLKVKYF SPEEEFN
Validated NT Sequence
attgttaaac tcttcttcag gactaaaata ttttacttta agttttaaac ttttagcagt tagctcacca gttaacaatt tcatttgata tgaagcaagt actgaattct tataagcatc aacacgcgtt atttttgctt catacaattt ttgttcagca tctaaaacat caagtatagt tttagaacca actatttcct cttgcacaat accgttatat gatatttgtg cagcttctac tccttgatta gctgcaacaa tacgagattt tgctgtttca aatccttccc acacactaac aacacctgct tttatctgct ttatggcact atcaagctgg actacactat tccttgtttg atttttagct gatcttattc ttgaatattg cgctcctcct tcaggataaa tagggatatt taccgaaaga gtagtagtat agatcctatt gtttatattt tgaataactg gctcttgtgg attataatta gtcccccctg atagcaactt tacacttact tgcggcaata atttcccttt ttgcaccatt tctaaagctt tagtaacagt tacgttatgc cttgctgaat taatatcagg attgaacttc gcagcttttc ttgtgaactc atctaacgaa ataggtaacc tatcaggtaa atcaggcata gttatatcat ttgcctctat tccaaacact ttaataaaat ttgctttttt cccttgaaaa tctgcataag cagctagttt atttgtttct gctgctgcaa gtcctgctct tgcagcagct atatctattg ctgttgcttc acctagtctt aatttctctt caacagtctt aacttgctgt atattagtac gtaccctact ttcagagata tcatatttct ccttactctc aacacaatca agataagcag ttattaaatt taataatact ttttgctcac cagcatagta ttcggatcgt gacgctctaa atcctgattg agcagcttta agagcagcaa tactagcacc gccattaaat aatgattgtt caattgttaa tatcccttga gtgctagctg tctcacgtgg agtaatacca agcctattaa catatttttt attatattta gtcttactat tttgtctatt aatctgtaaa cctacattag gcataaaccc tgagaaagct tgaggaaact gttcaattgc attcaaaaac ttaatttgag cagctttcag ttcctcatta ttcttatatc cttcagttaa agcttgctcc aaatcaa
Expected Protein Sequence
MAHHHHHHMA LDLEQALTEG YKNNEELKAA QIKFLNAIEQ FPQAFSGFMP NVGLQINRQN SKTKYNKKYV NRLGITPRET ASTQGILTIE QSLFNGGASI AALKAAQSGF RASRSEYYAG EQKVLLNLIT AYLDCVESKE KYDISESRVR TNIQQVKTVE EKLRLGEATA IDIAAARAGL AAAETNKLAA YADFQGKKAN FIKVFGIEAN DITMPDLPDR LPISLDEFTR KAAKFNPDIN SARHNVTVTK ALEMVQKGKL LPQVSVKLLS GGTNYNPQEP VIQNINNRIY TTTLSVNIPI YPEGGAQYSR IRSAKNQTRN SVVQLDSAIK QIKAGVVSVW EGFETAKSRI VAANQGVEAA QISYNGIVQE EIVGSKTILD VLDAEQKLYE AKITRVDAYK NSVLASYQMK LLTGELTAKS LKLKVKYFSP EEEFNNLKKK MFIGF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctc ttgatttgga gcaagcttta actgaaggat ataagaataa tgaggaactg aaagctgctc aaattaagtt tttgaatgca attgaacagt ttcctcaagc tttctcaggg tttatgccta atgtaggttt acagattaat agacaaaata gtaagactaa atataataaa aaatatgtta ataggcttgg tattactcca cgtgagacag ctagcactca agggatatta acaattgaac aatcattatt taatggcggt gctagtattg ctgctcttaa agctgctcaa tcaggattta gagcgtcacg atccgaatac tatgctggtg agcaaaaagt attattaaat ttaataactg cttatcttga ttgtgttgag agtaaggaga aatatgatat ctctgaaagt agggtacgta ctaatataca gcaagttaag actgttgaag agaaattaag actaggtgaa gcaacagcaa tagatatagc tgctgcaaga gcaggacttg cagcagcaga aacaaataaa ctagctgctt atgcagattt tcaagggaaa aaagcaaatt ttattaaagt gtttggaata gaggcaaatg atataactat gcctgattta cctgataggt tacctatttc gttagatgag ttcacaagaa aagctgcgaa gttcaatcct gatattaatt cagcaaggca taacgtaact gttactaaag ctttagaaat ggtgcaaaaa gggaaattat tgccgcaagt aagtgtaaag ttgctatcag gggggactaa ttataatcca caagagccag ttattcaaaa tataaacaat aggatctata ctactactct ttcggtaaat atccctattt atcctgaagg aggagcgcaa tattcaagaa taagatcagc taaaaatcaa acaaggaata gtgtagtcca gcttgatagt gccataaagc agataaaagc aggtgttgtt agtgtgtggg aaggatttga aacagcaaaa tctcgtattg ttgcagctaa tcaaggagta gaagctgcac aaatatcata taacggtatt gtgcaagagg aaatagttgg ttctaaaact atacttgatg ttttagatgc tgaacaaaaa ttgtatgaag caaaaataac gcgtgttgat gcttataaga attcagtact tgcttcatat caaatgaaat tgttaactgg tgagctaact gctaaaagtt taaaacttaa agtaaaatat tttagtcctg aagaagagtt taacaatctt aaaaagaaaa tgtttatagg tttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat