SSGCID: Target Page

RiprA.10100.b

PROTEASE DO (htrA)

CENTER ID:	RiprA.10100.b
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.10100.b.A2.GE27749	Mature protein( RiprA.10100.b )		26	315

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_133460
PATRIC ID:	fig\|272947.5.peg.193
RefSeq:	NP_220575.1
UniProt:	Q9ZDX8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MILQEVKMKK LPILLKLVFI INLVFTNNIV LASSDKNTAI FEKAKKAIVT IETRIAVSAY DDTSSWTGTG FINDKNNGYI ITNTHVVGGA SIGTYFVTFY NGEQAEAKLI YYDIWQDYAI LKVESKDIPV SAIQIAFTSE LPKLNQKVFV VGNTEAKGFS FHTGYLSDLY HIEGLMPQCT YVINLNTTGG ASGSPVLNDK IEAIGVLYGG GKTHSLALHG DYVARTLKSL KNNKQPSRKH IGIISELYSL NKAVRHHNFP KDEMYKYINK FPDSRNRVIS VKAVLSGSPA QKSLKAGDII WAVNDQETWR QFSII

NT Sequence

GTGATTTTAC AAGAGGTAAA AATGAAAAAA TTACCGATTT TACTAAAACT TGTATTTATT ATTAATTTAG TTTTTACGAA TAATATAGTT TTAGCATCTT CTGATAAAAA CACTGCAATA TTTGAAAAAG CTAAAAAAGC AATCGTAACG ATTGAGACTA GAATTGCAGT ATCGGCTTAT GATGATACAA GTAGTTGGAC TGGAACAGGA TTTATTAACG ATAAGAATAA CGGTTATATA ATTACTAATA CTCATGTTGT TGGTGGTGCA TCTATCGGAA CTTATTTTGT TACTTTTTAT AACGGTGAAC AGGCAGAAGC AAAACTTATC TATTACGATA TTTGGCAAGA TTATGCTATT TTGAAAGTAG AGAGTAAAGA TATACCGGTT TCAGCAATAC AAATAGCTTT TACTAGTGAG CTTCCAAAAT TAAATCAAAA GGTTTTTGTA GTTGGTAATA CAGAGGCGAA AGGTTTTTCT TTCCATACTG GTTATTTATC CGATCTTTAT CATATTGAAG GTTTAATGCC CCAATGTACT TATGTTATTA ACCTTAATAC TACTGGTGGT GCTAGTGGTT CGCCTGTATT AAATGATAAG ATTGAGGCTA TAGGAGTATT ATATGGTGGC GGTAAAACTC ATTCTTTAGC ATTACACGGT GATTATGTAG CACGTACTTT GAAAAGTTTA AAAAATAATA AACAGCCAAG CAGGAAACAT ATAGGAATAA TTAGCGAGCT GTATTCGTTA AATAAAGCAG TTAGACATCA TAATTTTCCT AAAGACGAAA TGTATAAATA TATAAATAAA TTTCCTGATA GTAGAAATAG AGTGATAAGT GTTAAGGCAG TTTTATCTGG TTCTCCTGCT CAAAAATCTT TAAAAGCAGG TGATATTATT TGGGCAGTAA ATGATCAAGA AACTTGGCGG CAATTTAGCA TTATT

Details for RiprA.10100.b.A2.GE27749

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTNNIVLAS SDKNTAIFEK AKKAIVTIET RIAVSAYDDT SSWTGTGFIN DKNNGYIITN THVVGGASIG TYFVTFYNGE QAEAKLIYYD IWQDYAILKV ESKDIPVSAI QIAFTSELPK LNQKVFVVGN TEAKGFSFHT GYLSDLYHIE GLMPQCTYVI NLNTTGGASG SPVLNDKIEA IGVLYGGGKT HSLALHGDYV ARTLKSLKNN KQPSRKHIGI ISELYSLNKA VRHHNFPKDE MYKYINKFPD SRNRVISVKA VLSGSPAQKS LKAGDIIWAV NDQETWRQFS II

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacga ataatatagt tttagcatct tctgataaaa acactgcaat atttgaaaaa gctaaaaaag caatcgtaac gattgagact agaattgcag tatcggctta tgatgataca agtagttgga ctggaacagg atttattaac gataagaata acggttatat aattactaat actcatgttg ttggtggtgc atctatcgga acttattttg ttacttttta taacggtgaa caggcagaag caaaacttat ctattacgat atttggcaag attatgctat tttgaaagta gagagtaaag atataccggt ttcagcaata caaatagctt ttactagtga gcttccaaaa ttaaatcaaa aggtttttgt agttggtaat acagaggcga aaggtttttc tttccatact ggttatttat ccgatcttta tcatattgaa ggtttaatgc cccaatgtac ttatgttatt aaccttaata ctactggtgg tgctagtggt tcgcctgtat taaatgataa gattgaggct ataggagtat tatatggtgg cggtaaaact cattctttag cattacacgg tgattatgta gcacgtactt tgaaaagttt aaaaaataat aaacagccaa gcaggaaaca tataggaata attagcgagc tgtattcgtt aaataaagca gttagacatc ataattttcc taaagacgaa atgtataaat atataaataa atttcctgat agtagaaata gagtgataag tgttaaggca gttttatctg gttctcctgc tcaaaaatct ttaaaagcag gtgatattat ttgggcagta aatgatcaag aaacttggcg gcaatttagc attatttaat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgaataat atagttttag catcttctga taaaaacact gcaatatttg aaaaagctaa aaaagcaatc gtaacgattg agactagaat tgcagtatcg gcttatgatg atacaagtag ttggactgga acaggattta ttaacgataa gaataacggt tatataatta ctaatactca tgttgttggt ggtgcatcta tcggaactta ttttgttact ttttataacg gtgaacaggc agaagcaaaa cttatctatt acgatatttg gcaagattat gctattttga aagtagagag taaagatata ccggtttcag caatacaaat agcttttact agtgagcttc caaaattaaa tcaaaaggtt tttgtagttg gtaatacaga ggcgaaaggt ttttctttcc atactggtta tttatccgat ctttatcata ttgaaggttt aatgccccaa tgtacttatg ttattaacct taatactact ggtggtgcta gtggttcgcc tgtattaaat gataagattg aggctatagg agtattatat ggtggcggta aaactcattc tttagcatta cacggtgatt atgtagcacg tactttgaaa agtttaaaaa ataataaaca gccaagcagg aaacatatag gaataattag cgagctgtat tcgttaaata aagcagttag acatcataat tttcctaaag acgaaatgta taaatatata aataaatttc ctgatagtag aaatagagtg ataagtgtta aggcagtttt atctggttct cctgctcaaa aatctttaaa agcaggtgat attatttggg cagtaaatga tcaagaaact tggcggcaat ttagcattat taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa