SSGCID: Target Page

RiprA.10014.d

Cytochrome c biogenesis ATP-binding export protein CcmA

CENTER ID:	RiprA.10014.d
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.10014.d.B1.GE35313	Full length( RiprA.10014.d )		1	197

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272947.5.peg.830
RefSeq:	NP_221144.1
UniProt:	Q9ZCF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDMLSLNQLQ LNIEKRNLFD LCITFLPSAI TYIKGANGSG KSSLLRMIAG IMQPSSGNIY YKNSHINNCI KPYCTYIGHN LGLKLEMTVF ENLKFWSEIY NSTETVPAAI HYFKLDYLLD EKCYNLSSGI QKIVAIARLI VCQSDLWLLD EVESNLSKEN KDLLNNLIVM KANSGGIILL SSHQENVIKS AQILQLK

NT Sequence

ATGGATATGT TATCACTTAA TCAATTACAA TTAAACATAG AAAAAAGAAA TTTATTTGAT CTATGTATTA CATTTCTTCC TTCTGCTATA ACTTATATAA AAGGTGCTAA TGGTTCTGGA AAAAGTTCGC TATTGCGTAT GATAGCCGGG ATTATGCAAC CAAGTAGCGG TAATATCTAC TATAAAAATA GTCATATCAA CAATTGCATT AAACCTTACT GCACTTATAT AGGGCATAAT TTAGGTCTAA AACTCGAAAT GACTGTTTTT GAAAATCTGA AATTTTGGTC AGAAATTTAT AATTCTACTG AAACCGTACC AGCTGCCATT CATTACTTCA AACTAGACTA TTTACTAGAT GAAAAATGCT ATAACTTATC TAGCGGAATA CAGAAAATTG TAGCTATCGC AAGACTTATT GTATGCCAAT CAGATTTATG GTTACTTGAT GAAGTTGAAA GTAACTTAAG CAAAGAAAAT AAAGATTTAC TGAATAATTT AATTGTCATG AAAGCAAATA GCGGTGGTAT TATTCTTCTA TCCTCACATC AAGAAAATGT TATAAAATCT GCACAGATAT TACAGCTTAA G

Details for RiprA.10014.d.B1.GE35313

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD MLSLNQLQLN IEKRNLFDLC ITFLPSAITY IKGANGSGKS SLLRMIAGIM QPSSGNIYYK NSHINNCIKP YCTYIGHNLG LKLEMTVFEN LKFWSEIYNS TETVPAAIHY FKLDYLLDEK CYNLSSGIQK IVAIARLIVC QSDLWLLDEV ESNLSKENKD LLNNLIVMKA NSGGIILLSS HQENVIKSAQ ILQLK

Validated NT Sequence

anattttnnt taactttaag aaggaganat accatggctc accaccacca ccaccatatg gatatgttat cacttaatca attacaatta aacatagaaa aaagaaattt atttgatcta tgtattacat ttcttccttc tgctataact tatataaaag gtgctaatgg ttctggaaaa agttcgctat tgcgtatgat agccgggatt atgcaaccaa gtagcggtaa tatctactat aaaaatagtc atatcaacaa ttgcattaaa ccttactgca cttatatagg gcataattta ggtctaaaac tcgaaatgac tgtttttgaa aatctgaaat tttggtcaga aatttataat tctactgaaa ccgtaccagc tgccattcat tacttcaaac tagactattt actagatgaa aaatgctata acttatctag cggaatacag aaaattgtag ctatcgcaag acttattgta tgccaatcag atttatggtt acttgatgaa gttgaaagta acttaagcaa agaaaataaa gatttactga ataatttaat tgtcatgaaa gcaaatagcg gtggtattat tcttctatcc tcacatcaag aaaatgttat aaaatctgca cagatattac agcttaagta agtgagtaag ataggatccg gctgnnnnnn nncccccnnn nnnnnnnnnn nnannnnnnn nannnnnnnn tnnntcncnc ctgaccntnn ncattnagtg nnnnnannnn nccggctgnn nnnnnncc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata tgttatcact taatcaatta caattaaaca tagaaaaaag aaatttattt gatctatgta ttacatttct tccttctgct ataacttata taaaaggtgc taatggttct ggaaaaagtt cgctattgcg tatgatagcc gggattatgc aaccaagtag cggtaatatc tactataaaa atagtcatat caacaattgc attaaacctt actgcactta tatagggcat aatttaggtc taaaactcga aatgactgtt tttgaaaatc tgaaattttg gtcagaaatt tataattcta ctgaaaccgt accagctgcc attcattact tcaaactaga ctatttacta gatgaaaaat gctataactt atctagcgga atacagaaaa ttgtagctat cgcaagactt attgtatgcc aatcagattt atggttactt gatgaagttg aaagtaactt aagcaaagaa aataaagatt tactgaataa tttaattgtc atgaaagcaa atagcggtgg tattattctt ctatcctcac atcaagaaaa tgttataaaa tctgcacaga tattacagct taagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat