RiprA.00738.a

RNA polymerase sigma factor

CENTER ID: RiprA.00738.a
ORGANISM: Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE: Endemic typhus
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiprA.00738.a.A1.GE31079 Full length( RiprA.00738.a ) 1 293
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272947.5.peg.312
RefSeq: NP_220687.1
UniProt: Q9ZDM4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNNINALAI SSESGFYSYL QKINKIPSLT QEEEFLLAKS YLEENDLQAA HKLVTSHLKL VAKIASSYKT YGLPITELVS EGNIGLMQAV KKFNPELGFR LSTYAMWWIK ASIQEYILKS WSLVKMGTTA AQKKLFFSLN KVKHKITNLY SRAITTDDFA QIADELGVSV NEVSEMNTRI SGPDLSLNNS INSDDVESGE LIELLPELRP TPEAMAINKQ NYTSKRKLLS NAMQILNDRE LRILKDRKLT DTPKTLDILS SEYNISKERI RQIENTAFEK IKKFILNNNR EIA
NT Sequence
ATGACTAATA ATATTAATGC ATTAGCAATT TCTAGTGAAT CAGGATTTTA TAGCTATTTA CAAAAAATCA ATAAAATACC TTCACTTACT CAAGAAGAAG AATTTTTATT GGCTAAATCT TATTTAGAGG AAAATGATTT ACAAGCTGCA CATAAATTAG TAACGAGTCA TCTTAAACTT GTTGCAAAAA TTGCAAGCAG CTACAAAACT TACGGTCTTC CTATTACCGA ATTGGTTTCA GAAGGTAATA TAGGATTAAT GCAGGCAGTA AAAAAATTCA ATCCGGAACT TGGTTTTCGC CTTTCAACAT ATGCGATGTG GTGGATTAAA GCATCTATAC AAGAATATAT ATTAAAATCT TGGTCATTAG TAAAAATGGG AACAACAGCA GCACAAAAAA AATTATTTTT TAGTCTTAAT AAAGTGAAGC ATAAAATTAC TAATTTATAC TCAAGGGCTA TTACTACTGA TGATTTTGCA CAAATAGCAG ATGAGTTAGG CGTTTCAGTG AATGAGGTGT CGGAGATGAA TACTAGAATT TCAGGTCCTG ATTTGTCGTT AAATAACTCT ATCAATAGTG ATGATGTAGA ATCAGGCGAA TTAATAGAAC TATTACCGGA ACTACGGCCT ACTCCTGAAG CTATGGCAAT TAACAAGCAA AATTACACAA GTAAAAGAAA ATTACTATCA AATGCTATGC AGATTTTAAA TGATAGGGAA TTACGTATTT TAAAAGATCG TAAATTAACA GATACTCCTA AAACTCTAGA TATTTTAAGC AGTGAGTATA ATATTTCGAA GGAACGTATT AGACAAATAG AAAATACAGC TTTTGAAAAA ATAAAAAAGT TTATTTTAAA CAATAATAGA GAAATCGCT
Details for RiprA.00738.a.A1.GE31079
HARVESTED ON: 12/21/2010
SEQUENCED ON: 1/13/2011
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 95
PERCENT COVERAGE: 90
Validated AA Sequence
MGTLEAQTQG PGSMTNNINA LAISSESGFY SYLQKINKIP SLTQEEXFLL AKSYLEENDL QAAHXXXTSH LKLVAKIASS YKTYGLPITE LVSEGNIGLM QAVKKFNPEL GFRLSTYAMW WIKASIQEYI LKSWSLVKMG TTAAQKKLFF SLNKVKHKIT NLYSRAITTD DFAQIADELG VSVNEVSEMN TRISGPDLSL NNSINSDDVE SGELIELLPE LRPTPEAMAI NKQNYTXKRK LLSNAMQILN DRELXILKDR XLTDTPXTLD ILSSEYXISK XRXXX
Validated NT Sequence
cnnnnnnnnn nnnnnnnnnn nngcgnnnnn nccatgtnnn nnnnnnnncc ntgnnnnnnc nccntcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacta ataatattaa tgcattagca atttctagtg aatcaggatt ttatagctat ttacaaaaaa tcaataaaat accttcactt actcaagaag aanaattttt attggctaaa tcttatttag aggaaaatga tttacaagct gcacataann anntaacgag tcatcttaaa cttgttgcaa aaattgcaag cagctacaaa acttacggtc ttcctattac cgaattggtt tcagaaggta atataggatt aatgcaggca gtaaaaaaat tcaatccgga acttggtttt cgcctttcaa catatgcgat gtggtggatt aaagcatcta tacaagaata tatattaaaa tcttggtcat tagtaaaaat gggaacaaca gcagcacaaa aaaaattatt ttttagtctt aataaagtga agcataaaat tactaattta tactcaaggg ctattactac tgatgatttt gcacaaatag cagatgagtt aggcgtttca gtgaatgagg tgtcggagat gaatactaga atttcaggtc ctgatttgtc gttaaataac tctatcaata gtgatgatgt agaatcaggc gaattaatag aactattacc ggaactacgg cctactcctg aagctatggc aattaacaag caaaattaca cnngtaaaag aaaattacta tcaaatgcta tgcagatttt aaatgatagg gaattacnta ttttaaaaga tcgtanatta acagatactc ctannactct agatatttta agcagtgagt atnatatttc gaaggnncgt antanacant agaaaanaca gcttttgaaa aaataaaaaa agnttatttt naacaanaat agannaaatc gcttnnnanc ngcacgaacn annnngcagn nnnnnnnncg annnncnnct gctannaagn ngaangnann ctnanttngn tgntgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNNINALA ISSESGFYSY LQKINKIPSL TQEEEFLLAK SYLEENDLQA AHKLVTSHLK LVAKIASSYK TYGLPITELV SEGNIGLMQA VKKFNPELGF RLSTYAMWWI KASIQEYILK SWSLVKMGTT AAQKKLFFSL NKVKHKITNL YSRAITTDDF AQIADELGVS VNEVSEMNTR ISGPDLSLNN SINSDDVESG ELIELLPELR PTPEAMAINK QNYTSKRKLL SNAMQILNDR ELRILKDRKL TDTPKTLDIL SSEYNISKER IRQIENTAFE KIKKFILNNN REIA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactaataat attaatgcat tagcaatttc tagtgaatca ggattttata gctatttaca aaaaatcaat aaaatacctt cacttactca agaagaagaa tttttattgg ctaaatctta tttagaggaa aatgatttac aagctgcaca taaattagta acgagtcatc ttaaacttgt tgcaaaaatt gcaagcagct acaaaactta cggtcttcct attaccgaat tggtttcaga aggtaatata ggattaatgc aggcagtaaa aaaattcaat ccggaacttg gttttcgcct ttcaacatat gcgatgtggt ggattaaagc atctatacaa gaatatatat taaaatcttg gtcattagta aaaatgggaa caacagcagc acaaaaaaaa ttatttttta gtcttaataa agtgaagcat aaaattacta atttatactc aagggctatt actactgatg attttgcaca aatagcagat gagttaggcg tttcagtgaa tgaggtgtcg gagatgaata ctagaatttc aggtcctgat ttgtcgttaa ataactctat caatagtgat gatgtagaat caggcgaatt aatagaacta ttaccggaac tacggcctac tcctgaagct atggcaatta acaagcaaaa ttacacaagt aaaagaaaat tactatcaaa tgctatgcag attttaaatg atagggaatt acgtatttta aaagatcgta aattaacaga tactcctaaa actctagata ttttaagcag tgagtataat atttcgaagg aacgtattag acaaatagaa aatacagctt ttgaaaaaat aaaaaagttt attttaaaca ataatagaga aatcgcttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa