RiprA.00532.a

Thymidylate synthase

CENTER ID: RiprA.00532.a
ORGANISM: Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE: Endemic typhus
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiprA.00532.a.D11.GE00001 RiprA.00532.a 1 294
RiprA.00532.a.D12.GE00001 RiprA.00532.a 1 247
RiprA.00532.a.D13.GE00001 RiprA.00532.a 1 253
RiprA.00532.a.D14.GE00001 RiprA.00532.a 18 294
RiprA.00532.a.D15.GE00001 RiprA.00532.a 18 247
RiprA.00532.a.D16.GE00001 RiprA.00532.a 18 253
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_155488
PATRIC ID: fig|272947.5.peg.310
RefSeq: NP_220685.1
UniProt: Q9ZDM6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHNTTKRVTV PALEAMLYET IKVLDHGFIR VIDYMGDDSS IVQAARVSYG KGTKQLNQDK GLINYLLRHY HTTPFEMCDI KFHIKLPIFI ARQWIRHRTA SVNEYSARYS ILGNEFYLPD PANIASQSVV NKQCRAGDSV PKKVSEKVLA ILEEDARRCY RHYKELMNAD EDGNILDENV SGIARELARI NLTLNYYTEW YWKINLHNLL HFLRLRTDPK AQYEIRVYAE KILDIVKAWV PFTYEAFEEY RLQGANISRK GLEVIKRMIK GEKVIHETSG MNKREWEELV KIFR
NT Sequence
atgcacaata cgacaaaaag agtaacagta ccagcacttg aagcaatgtt atatgagact atcaaagttt tagatcatgg ttttattagg gtaatcgatt atatgggtga tgatagttcg atagtacagg cagctcgtgt ttcttatggt aaaggtacta agcagttaaa ccaagataaa ggattaataa attacttgct gcgtcattat catacgacac cttttgagat gtgtgacatc aaatttcaca ttaaattacc tatatttatt gcacggcagt ggattaggca taggactgct agtgttaacg aatattcagc aaggtattct attttaggaa atgaatttta tttacctgac cctgccaata ttgcttctca atctgttgta aataaacaat gtagagcagg ggatagcgta ccgaaaaaag tatctgaaaa agttctcgca attttagaag aagatgctag acggtgctac aggcattata aggagctcat gaatgctgat gaagatggaa atattctaga tgagaatgtt tcaggcatag caagagaact tgctcgtatt aatttaactt tgaattatta tacggaatgg tattggaaga ttaatttaca taatttgctt cattttttaa gattacgaac tgatcctaag gcacaatatg aaattagagt ttatgccgaa aagatacttg acatagtaaa agcttgggta ccttttactt acgaagcgtt tgaagagtat cgtttgcaag gcgcgaatat ttcacgtaaa ggtttagagg taattaaaag aatgataaaa ggtgaaaaag tgatccatga aactagtggt atgaataaaa gagaatggga ggaattggta aagattttta ggtag
Details for RiprA.00532.a.D11.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/31/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 88
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG MHNTTKRVTV PALEAMLYET IKVLDHGFIR VIDYMGDDSS IVQAARVSYG KGTKQLNQDK GLINYLLRHY HTTPFEMCDI KFHIKLPIFI ARQWIRHRTA SVNEYSARYS ILGNEFYLPD PANIASQSVV NKQCRAGDSV PKKVSEKVLA ILEEDARRCY RHYKELMNAD EDGNILDENV SGIARELARI NLTLNYYTEW YWKINLHNLL HFLRLRTDPK AQYEIRVYAE KILDIVKAWV PFTYEAFEEY RLQGANISRK GLEVIKRMIK GEKVIHETSG MNKREWEELV KIFR
Validated NT Sequence
gcggcgaagt aaaaccggaa gtgaagccgg agacccacat caacctgaag gttagcgacg gtagcagcga aatcttcttc aagattaaga agaccacccc gctgcgtcgc ctgatggaag cattcgctaa acgccagggc aaggaaatgg attccctgcg ctttctgtac gacggtatcc gtattcaggc agaccagact ccggaagatc tggacatgga agataacgac attatcgaag cacaccgtga acaaatcggt ggaatgcaca acaccacgaa acgcgtaacg gtgccagctc tggaggcaat gctgtacgag actatcaagg tcctggacca cggctttatt cgtgtgatcg attatatggg cgacgattcc tccatcgtgc aagctgctcg tgtttcctac ggcaaaggta ccaaacagct gaaccaagac aaaggtctga ttaactatct cctgcgtcat tatcacacca ctcctttcga gatgtgtgac atcaagttcc atatcaaact gccaatcttc atagctcgtc agtggattcg tcaccgcact gcatctgtca acgaatatag cgctcgttat tctatcctcg gcaacgaatt ctatctcccg gacccggcca acatagcttc gcagtccgtt gtaaacaagc agtgccgtgc cggcgattct gttccgaaga aagtaagcga gaaggtatta gctatcctgg aagaggacgc ccgccgctgt taccgtcact ataaagaatt aatgaacgcc gacgaagacg gcaatatcct tgacgagaac gtctccggca tcgcacgtga actcgcccgc atcaatctga ctctcaacta ctacaccgaa tggtactgga agattaacct gcataacctc ctgcactttc tccgtctgcg tactgacccg aaggcacaat acgagattcg tgtctacgct gagaagattc tggacatagt taaagcatgg gttcctttca cctacgaagc attcgaagag tatcgtctgc agggcgcaaa catctctcgt aaaggccttg aagtcattaa gcgtatgatc aaaggcgaga aagtgatcca tgaaacctct ggtatgaata aacgcgaatg ggaagaactc gtcaagattt ttcgttaa
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMHNT TKRVTVPALE AMLYETIKVL DHGFIRVIDY MGDDSSIVQA ARVSYGKGTK QLNQDKGLIN YLLRHYHTTP FEMCDIKFHI KLPIFIARQW IRHRTASVNE YSARYSILGN EFYLPDPANI ASQSVVNKQC RAGDSVPKKV SEKVLAILEE DARRCYRHYK ELMNADEDGN ILDENVSGIA RELARINLTL NYYTEWYWKI NLHNLLHFLR LRTDPKAQYE IRVYAEKILD IVKAWVPFTY EAFEEYRLQG ANISRKGLEV IKRMIKGEKV IHETSGMNKR EWEELVKIFR
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gcacaacacc acgaaacgcg taacggtgcc agctctggag gcaatgctgt acgagactat caaggtcctg gaccacggct ttattcgtgt gatcgattat atgggcgacg attcctccat cgtgcaagct gctcgtgttt cctacggcaa aggtaccaaa cagctgaacc aagacaaagg tctgattaac tatctcctgc gtcattatca caccactcct ttcgagatgt gtgacatcaa gttccatatc aaactgccaa tcttcatagc tcgtcagtgg attcgtcacc gcactgcatc tgtcaacgaa tatagcgctc gttattctat cctcggcaac gaattctatc tcccggaccc ggccaacata gcttcgcagt ccgttgtaaa caagcagtgc cgtgccggcg attctgttcc gaagaaagta agcgagaagg tattagctat cctggaagag gacgcccgcc gctgttaccg tcactataaa gaattaatga acgccgacga agacggcaat atccttgacg agaacgtctc cggcatcgca cgtgaactcg cccgcatcaa tctgactctc aactactaca ccgaatggta ctggaagatt aacctgcata acctcctgca ctttctccgt ctgcgtactg acccgaaggc acaatacgag attcgtgtct acgctgagaa gattctggac atagttaaag catgggttcc tttcacctac gaagcattcg aagagtatcg tctgcagggc gcaaacatct ctcgtaaagg ccttgaagtc attaagcgta tgatcaaagg cgagaaagtg atccatgaaa cctctggtat gaataaacgc gaatgggaag aactcgtcaa gatttttcgt taaaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for RiprA.00532.a.D12.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/31/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 72
Validated AA Sequence
MHNTTKRVTV PALEAMLYET IKVLDHGFIR VIDYMGDDSS IVQAARVSYG KGTKQLNQDK GLINYLLRHY HTTPFEMCDI KFHIKLPIFI ARQWIRHRTA SVNEYSARYS ILGNEFYLPD PANIASQSVV NKQCRAGDSV PKKVSEKVLA ILEEDARRCY RHYKELMNAD EDGNILDENV SGIARELARI NLTLNYYTEW YWKINLHNLL HFLRLRTDPK AQYEIRVYAE KILDIVKAWV PFTYEAF
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMHNT TKRVTVPALE AMLYETIKVL DHGFIRVIDY MGDDSSIVQA ARVSYGKGTK QLNQDKGLIN YLLRHYHTTP FEMCDIKFHI KLPIFIARQW IRHRTASVNE YSARYSILGN EFYLPDPANI ASQSVVNKQC RAGDSVPKKV SEKVLAILEE DARRCYRHYK ELMNADEDGN ILDENVSGIA RELARINLTL NYYTEWYWKI NLHNLLHFLR LRTDPKAQYE IRVYAEKILD IVKAWVPFTY EAF
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gcacaacacc acgaaacgcg taacggtgcc agctctggag gcaatgctgt acgagactat caaggtcctg gaccacggct ttattcgtgt gatcgattat atgggcgacg attcctccat cgtgcaagct gctcgtgttt cctacggcaa aggtaccaaa cagctgaacc aagacaaagg tctgattaac tatctcctgc gtcattatca caccactcct ttcgagatgt gtgacatcaa gttccatatc aaactgccaa tcttcatagc tcgtcagtgg attcgtcacc gcactgcatc tgtcaacgaa tatagcgctc gttattctat cctcggcaac gaattctatc tcccggaccc ggccaacata gcttcgcagt ccgttgtaaa caagcagtgc cgtgccggcg attctgttcc gaagaaagta agcgagaagg tattagctat cctggaagag gacgcccgcc gctgttaccg tcactataaa gaattaatga acgccgacga agacggcaat atccttgacg agaacgtctc cggcatcgca cgtgaactcg cccgcatcaa tctgactctc aactactaca ccgaatggta ctggaagatt aacctgcata acctcctgca ctttctccgt ctgcgtactg acccgaaggc acaatacgag attcgtgtct acgctgagaa gattctggac atagttaaag catgggttcc tttcacctac gaagcattct aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for RiprA.00532.a.D13.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 12/29/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 86
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG MHNTTKRVTV PALEAMLYET IKVLDHGFIR VIDYMGDDSS IVQAARVSYG KGTKQLNQDK GLINYLLRHY HTTPFEMCDI KFHIKLPIFI ARQWIRHRTA SVNEYSARYS ILGNEFYLPD PANIASQSVV NKQCRAGDSV PKKVSEKVLA ILEEDARRCY RHYKELMNAD EDGNILDENV SGIARELARI NLTLNYYTEW YWKINLHNLL HFLRLRTDPK AQYEIRVYAE KILDIVKAWV PFTYEAFEEY RLQ
Validated NT Sequence
ttactgcaga cgatactctt cgaatgcttc gtaggtgaaa ggaacccatg ctttaactat gtccagaatc ttctcagcgt agacacgaat ctcgtattgt gccttcgggt cagtacgcag acggagaaag tgcaggaggt tatgcaggtt aatcttccag taccattcgg tgtagtagtt gagagtcaga ttgatgcggg cgagttcacg tgcgatgccg gagacgttct cgtcaaggat attgccgtct tcgtcggcgt tcattaattc tttatagtga cggtaacagc ggcgggcgtc ctcttccagg atagctaata ccttctcgct tactttcttc ggaacagaat cgccggcacg gcactgcttg tttacaacgg actgcgaagc tatgttggcc gggtccggga gatagaattc gttgccgagg atagaataac gagcgctata ttcgttgaca gatgcagtgc ggtgacgaat ccactgacga gctatgaaga ttggcagttt gatatggaac ttgatgtcac acatctcgaa aggagtggtg tgataatgac gcaggagata gttaatcaga cctttgtctt ggttcagctg tttggtacct ttgccgtagg aaacacgagc agcttgcacg atggaggaat cgtcgcccat ataatcgatc acacgaataa agccgtggtc caggaccttg atagtctcgt acagcattgc ctccagagct ggcaccgtta cgcgtttcgt ggtgttgtgc attccaccga tttgttcacg gtgtgcttcg ataatgtcgt tatcttccat gtccagatct tccggagtct ggtctgcctg aatacggata ccgtcgtaca gaaagcgcag ggaatccatt tccttgccct ggcgtttagc gaatgcttcc atcaggcgac gcagcggggt ggtcttctta atcttgaaga agatttcgct gctaccgtcg ctaaccttca ggttgatgtg ggtctccggc ttcacttccg gttttacttc gccgctatgg tggtggtggt g
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMHNT TKRVTVPALE AMLYETIKVL DHGFIRVIDY MGDDSSIVQA ARVSYGKGTK QLNQDKGLIN YLLRHYHTTP FEMCDIKFHI KLPIFIARQW IRHRTASVNE YSARYSILGN EFYLPDPANI ASQSVVNKQC RAGDSVPKKV SEKVLAILEE DARRCYRHYK ELMNADEDGN ILDENVSGIA RELARINLTL NYYTEWYWKI NLHNLLHFLR LRTDPKAQYE IRVYAEKILD IVKAWVPFTY EAFEEYRLQ
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gcacaacacc acgaaacgcg taacggtgcc agctctggag gcaatgctgt acgagactat caaggtcctg gaccacggct ttattcgtgt gatcgattat atgggcgacg attcctccat cgtgcaagct gctcgtgttt cctacggcaa aggtaccaaa cagctgaacc aagacaaagg tctgattaac tatctcctgc gtcattatca caccactcct ttcgagatgt gtgacatcaa gttccatatc aaactgccaa tcttcatagc tcgtcagtgg attcgtcacc gcactgcatc tgtcaacgaa tatagcgctc gttattctat cctcggcaac gaattctatc tcccggaccc ggccaacata gcttcgcagt ccgttgtaaa caagcagtgc cgtgccggcg attctgttcc gaagaaagta agcgagaagg tattagctat cctggaagag gacgcccgcc gctgttaccg tcactataaa gaattaatga acgccgacga agacggcaat atccttgacg agaacgtctc cggcatcgca cgtgaactcg cccgcatcaa tctgactctc aactactaca ccgaatggta ctggaagatt aacctgcata acctcctgca ctttctccgt ctgcgtactg acccgaaggc acaatacgag attcgtgtct acgctgagaa gattctggac atagttaaag catgggttcc tttcacctac gaagcattcg aagagtatcg tctgcagtaa aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
Details for RiprA.00532.a.D14.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/31/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 58
Validated AA Sequence
MCDIKFHIKL PIFIARQWIR HRXASVNEYS ARYSILGNEF YLPDPANIAS QSVVNKQCRA GDSVPKKVSE KVLAILEEDA RRCYRHYKEL MNADEDGNIL DENVSGIARE LARINLTLNY YTEWYWKINL HNLLHFLRLR TDPKAQYEIR VYAEKILDIV KAWVPFTYEA FEEYRLQGAN ISRKGLEVIK RMIKGEKVIH ETSGMNKREW EELVKIFR
Validated NT Sequence
ttcgggcttt gttagcagcc ggntctcngt ggtggtggtg gtggtgctcg agtgcggccg caagctttta acgaaaaatc ttgacgagtt cttcccattc gcgtttattc ataccagagg tttcatggat cactttctcg cctttgatca tacgcttaat gacttcaagg cctttacgag agatgtttgc gccctgcaga cgatactctt cgaatgcttc gtaggtgaaa ggaacccatg ctttaactat gtccagaatc ttctcagcgt agacacgaat ctcgtattgt gccttcgggt cagtacgcag acggagaaag tgcaggaggt tatgcaggtt aatcttccag taccattcgg tgtagtagtt gagagtcaga ttgatgcggg cgagttcacg tgcgatgccg gagacgttct cgtcaaggat attgccgtct tcgtcggcgt tcattaattc tttatagtga cggtaacagc ggcgggcgtc ctcttccagg atagctaata ccttctcgct tactttcttc ggaacagaat cgccggcacg gcactgcttg tttacaacgg actgcgaagc tatgttggcc gggtccggga gatagaattc gttgccgagg atagaataac gagcgctata ttcgttgaca gatgcagngc ggtgacgaat ccactgacga gctatgaaga ttggcagttt gatatggaac ttgatgtcac acatctcgaa anggagtggn gtgataatga cgcannannt nnnnnatcag anctttgtct tggttcanct gnttggtanc tttgccgtan gaaacacgag cagcttgcac ganggaggaa ncgncgccca tataatcgat nnnnngaata aagncgnggt ccaggacctt gnnagtctcg nanccnccga tttgttcnnn gnnnnnttcn ataagtcnnt nncttnnatg nnccannnct tccggantcn ggnctgncnn aannnanncn gnnnnacnnn aagcnnnagg nanncnattt ncnnnnncnn ngnntnnann naannnnnnn nnnnnnngnn nnnnnnnnnn nnntncngaa nnnnnattnn nnnnnnnnnn nnnnnnnntn nnggnnnnng nnnngnnnnn nnnnnnnnnn nnnnnncnnn ntnnnnnnnn nnnnng
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYETI KVLDHGFIRV IDYMGDDSSI VQAARVSYGK GTKQLNQDKG LINYLLRHYH TTPFEMCDIK FHIKLPIFIA RQWIRHRTAS VNEYSARYSI LGNEFYLPDP ANIASQSVVN KQCRAGDSVP KKVSEKVLAI LEEDARRCYR HYKELMNADE DGNILDENVS GIARELARIN LTLNYYTEWY WKINLHNLLH FLRLRTDPKA QYEIRVYAEK ILDIVKAWVP FTYEAFEEYR LQGANISRKG LEVIKRMIKG EKVIHETSGM NKREWEELVK IFR
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata cgagactatc aaggtcctgg accacggctt tattcgtgtg atcgattata tgggcgacga ttcctccatc gtgcaagctg ctcgtgtttc ctacggcaaa ggtaccaaac agctgaacca agacaaaggt ctgattaact atctcctgcg tcattatcac accactcctt tcgagatgtg tgacatcaag ttccatatca aactgccaat cttcatagct cgtcagtgga ttcgtcaccg cactgcatct gtcaacgaat atagcgctcg ttattctatc ctcggcaacg aattctatct cccggacccg gccaacatag cttcgcagtc cgttgtaaac aagcagtgcc gtgccggcga ttctgttccg aagaaagtaa gcgagaaggt attagctatc ctggaagagg acgcccgccg ctgttaccgt cactataaag aattaatgaa cgccgacgaa gacggcaata tccttgacga gaacgtctcc ggcatcgcac gtgaactcgc ccgcatcaat ctgactctca actactacac cgaatggtac tggaagatta acctgcataa cctcctgcac tttctccgtc tgcgtactga cccgaaggca caatacgaga ttcgtgtcta cgctgagaag attctggaca tagttaaagc atgggttcct ttcacctacg aagcattcga agagtatcgt ctgcagggcg caaacatctc tcgtaaaggc cttgaagtca ttaagcgtat gatcaaaggc gagaaagtga tccatgaaac ctctggtatg aataaacgcg aatgggaaga actcgtcaag atttttcgtt aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for RiprA.00532.a.D15.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/31/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 95
PERCENT COVERAGE: 85
Validated AA Sequence
MXAXAKRXGX EMDXXRXXYD GIRIQAXQTP EDLDMXDNDI IEAHRXQIGG XETIKVLDHG FIRVIDYMGD DSSIVQAARV SYGKGTKQLN QDKGLINYLL RHYHTTPFEM CDIKFHIKLP IFIARQWIRH RTASVNEYSA RYSILGNEFY LPDPANIASQ SVVNKQCRAG DSVPKKVSEK VLAILEEDAR RCYRHYKELM NADEDGNILD ENVSGIAREL ARINLTLNYY TEWYWKINLH NLLHFLRLRT DPKAQYEIRV YAEKILDIVK AWVPFTYEAF
Validated NT Sequence
ttcgggcttt gtnngcagcc gganctcngt ggtggtggtg gtggtgctcg agtgcggccg caagctttta gaatgcttcg taggtgaaag gaacccatgc tttaactatg tccagaatct tctcagcgta gacacgaatc tcgtattgtg ccttcgggtc agtacgcaga cggagaaagt gcaggaggtt atgcaggtta atcttccagt accattcggt gtagtagttg agagtcagat tgatgcgggc gagttcacgt gcgatgccgg agacgttctc gtcaaggata ttgccgtctt cgtcggcgtt cattaattct ttatagtgac ggtaacagcg gcgggcgtcc tcttccagga tagctaatac cttctcgctt actttcttcg gaacagaatc gccggcacgg cactgcttgt ttacaacgga ctgcgaagct atgttggccg ggtccgggag atagaattcg ttgccgagga tagaataacg agcgctatat tcgttgacag atgcagtgcg gtgacgaatc cactgacgag ctatgaagat tggcagtttg atatggaact tgatgtcaca catctcgaaa ggagtggtgt gataatgacg caggagatag ttaatcagac ctttgtcttg gttcagctgt ttggtacctt tgccgtanga aacacgagca gcttgcacga tggangaatc gtcgcccata taatcgatca cacgaataaa gccgtggtcc aggaccttga tagtctcgna tccaccgatt tgntcncggt gtgcttcgat aatgtcgtta tctnccatgt ccagatcttc cggagtctgg nctgcctgaa tacggatacc gtcgtacnna angcgnannn aatccatttc nntgcccngg cgtttagcgn angcttncat cnngcgacgc annggggtgg ncttnnaanc ttgaanaann ntngntgcta ccgncgctaa ccttcnngnt gannggnncn cnncttcnct nnnnnnnttn nntnnnngcn nnggngngnn ggnggtgnnn ngnnnnnncn nnnnnnnngn tannnannnt nnnnnntcnn nnnnnnnnt
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYETI KVLDHGFIRV IDYMGDDSSI VQAARVSYGK GTKQLNQDKG LINYLLRHYH TTPFEMCDIK FHIKLPIFIA RQWIRHRTAS VNEYSARYSI LGNEFYLPDP ANIASQSVVN KQCRAGDSVP KKVSEKVLAI LEEDARRCYR HYKELMNADE DGNILDENVS GIARELARIN LTLNYYTEWY WKINLHNLLH FLRLRTDPKA QYEIRVYAEK ILDIVKAWVP FTYEAF
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata cgagactatc aaggtcctgg accacggctt tattcgtgtg atcgattata tgggcgacga ttcctccatc gtgcaagctg ctcgtgtttc ctacggcaaa ggtaccaaac agctgaacca agacaaaggt ctgattaact atctcctgcg tcattatcac accactcctt tcgagatgtg tgacatcaag ttccatatca aactgccaat cttcatagct cgtcagtgga ttcgtcaccg cactgcatct gtcaacgaat atagcgctcg ttattctatc ctcggcaacg aattctatct cccggacccg gccaacatag cttcgcagtc cgttgtaaac aagcagtgcc gtgccggcga ttctgttccg aagaaagtaa gcgagaaggt attagctatc ctggaagagg acgcccgccg ctgttaccgt cactataaag aattaatgaa cgccgacgaa gacggcaata tccttgacga gaacgtctcc ggcatcgcac gtgaactcgc ccgcatcaat ctgactctca actactacac cgaatggtac tggaagatta acctgcataa cctcctgcac tttctccgtc tgcgtactga cccgaaggca caatacgaga ttcgtgtcta cgctgagaag attctggaca tagttaaagc atgggttcct ttcacctacg aagcattcta aaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for RiprA.00532.a.D16.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/31/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 86
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIXADQTP EDLDMEDNDI IEAHREQIGG YETIKVLDHG FIRVIDYMGD DSSIVQAARV SYGKGTKQLN QDKGLINYLL RHYHTTPFEM CDIKFHIKLP IFIARQXIRH RTXXVNEYSA RYSILGNEFY LPDPANIASQ SVVNKQCRAG DSVPKKVSEK VLAILEEDAR RCYRHYKELM NADEDGNILD ENVSGIAREL ARINLTLNYY TEWYWKINLH NLLHFLRLRT DPKAQYEIRV YAEKILDIVK AWVPFTYEAF EEYRLQ
Validated NT Sequence
ttactgcaga cgatactctt cgaatgcttc gtaggtgaaa ggaacccatg ctttaactat gtccagaatc ttctcagcgt agacacgaat ctcgtattgt gccttcgggt cagtacgcag acggagaaag tgcaggaggt tatgcaggtt aatcttccag taccattcgg tgtagtagtt gagagtcaga ttgatgcggg cgagttcacg tgcgatgccg gagacgttct cgtcaaggat attgccgtct tcgtcggcgt tcattaattc tttatagtga cggtaacagc ggcgggcgtc ctcttccagg atagctaata ccttctcgct tactttcttc ggaacagaat cgccggcacg gcactgcttg tttacaacgg actgcgaagc tatgttggcc gggtccggga gatagaattc gttgccgagg atagaataac gagcgctata ttcgttgaca gnnncagtgc ggtgacgaat ccnctgacga gctatgaaga ttggcagttt gatatggaac ttgatgtcac acatctcgaa aggagtggtg tgataatgac gcaggagata gttaatcaga cctttgtctt ggttcagctg tttggtacct ttgccgtagg aaacacgagc agcttgcacg atggaggaat cgtcgcccat ataatcgatc acacgaataa agccgtggtc caggaccttg atagtctcgt atccaccgat ttgttcncgg tgtgcttcga taatgtcgtt atcttccatg tccagatctt ccggagtctg gtctgccnga atacggatac cgtcgtacag aaagcgcagg gaatccattt ccttgccctg gcgtttagcg aatgcttcca tcaggcgacg cagcggggtg gtcttcttaa tcttgaagaa gatttcgctg ctaccgtcgc taaccttcag gttgatgtgg gtctccggct tcacttccgg ttttacttcg ccgctangg
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYETI KVLDHGFIRV IDYMGDDSSI VQAARVSYGK GTKQLNQDKG LINYLLRHYH TTPFEMCDIK FHIKLPIFIA RQWIRHRTAS VNEYSARYSI LGNEFYLPDP ANIASQSVVN KQCRAGDSVP KKVSEKVLAI LEEDARRCYR HYKELMNADE DGNILDENVS GIARELARIN LTLNYYTEWY WKINLHNLLH FLRLRTDPKA QYEIRVYAEK ILDIVKAWVP FTYEAFEEYR LQ
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata cgagactatc aaggtcctgg accacggctt tattcgtgtg atcgattata tgggcgacga ttcctccatc gtgcaagctg ctcgtgtttc ctacggcaaa ggtaccaaac agctgaacca agacaaaggt ctgattaact atctcctgcg tcattatcac accactcctt tcgagatgtg tgacatcaag ttccatatca aactgccaat cttcatagct cgtcagtgga ttcgtcaccg cactgcatct gtcaacgaat atagcgctcg ttattctatc ctcggcaacg aattctatct cccggacccg gccaacatag cttcgcagtc cgttgtaaac aagcagtgcc gtgccggcga ttctgttccg aagaaagtaa gcgagaaggt attagctatc ctggaagagg acgcccgccg ctgttaccgt cactataaag aattaatgaa cgccgacgaa gacggcaata tccttgacga gaacgtctcc ggcatcgcac gtgaactcgc ccgcatcaat ctgactctca actactacac cgaatggtac tggaagatta acctgcataa cctcctgcac tttctccgtc tgcgtactga cccgaaggca caatacgaga ttcgtgtcta cgctgagaag attctggaca tagttaaagc atgggttcct ttcacctacg aagcattcga agagtatcgt ctgcagtaaa agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat