RiprA.00163.a

phenylalanyl-tRNA synthetase subunit alpha

CENTER ID:	RiprA.00163.a
ORGANISM:	Rickettsia prowazekii str. Madrid E
ASSOCIATED DISEASE:	Endemic typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiprA.00163.a.B1.GE27186	Full length( RiprA.00163.a )		1	350

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127036
PATRIC ID:	fig\|272947.5.peg.430
RefSeq:	NP_220798.1
UniProt:	Q9ZDB5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKNIETILKL AEEKIVLVHN LKDLQEYKVE FLGRNGIVTS ELKKLGSLIN GQKRKEFGLK INTLKDKIHD IIKAKAQNLE AEELHLKLAA DKIDLTIPAR RYKQGSIHPI TQCMDELIQV FSQFGFTIEN GPNIENDFYN FTALNFEYDH PARQMHDTFY LKGHENDKPL LLRTHTSTVQ IRAMKNGKPP FRFIAPGRTY RSDSDMTHTP MFHQIEGLVI DKNINMGHLK YVIITFIRSF FENSNIELRF RPSFFPFTEP SAEVDIRMNK NDKWLEVLGC GMVHPNVLKN IDINRSEYQG FAFGLGVERF AMLKYNIKDL RRFFEGDIRW LKHYNFESFD IPNLAGGLTK

NT Sequence

ATGAAAAATA TAGAAACAAT ATTAAAACTT GCTGAGGAAA AAATTGTATT AGTACATAAC TTAAAAGATT TGCAAGAATA TAAAGTAGAA TTTTTAGGTA GAAACGGTAT AGTAACGAGT GAGCTTAAAA AATTAGGTAG TTTAATAAAT GGACAAAAAC GTAAAGAATT CGGCTTAAAA ATCAACACAT TAAAAGATAA AATACACGAT ATAATAAAAG CAAAAGCGCA AAATTTAGAG GCAGAAGAAC TGCACTTAAA ACTTGCTGCC GATAAAATTG ATTTAACCAT TCCTGCAAGA AGATATAAAC AAGGTTCTAT TCATCCAATA ACTCAATGTA TGGATGAGTT AATACAAGTA TTTTCGCAGT TTGGTTTTAC TATAGAAAAT GGACCGAATA TAGAAAATGA TTTTTATAAT TTTACTGCTC TCAATTTTGA ATATGACCAT CCAGCAAGGC AGATGCACGA TACTTTTTAT TTAAAAGGTC ATGAAAATGA TAAACCATTG CTATTACGTA CTCACACCTC AACAGTTCAG ATTAGAGCAA TGAAAAACGG CAAACCGCCT TTTAGATTTA TAGCACCTGG TCGGACTTAT AGATCAGATT CAGATATGAC ACATACTCCA ATGTTCCACC AAATAGAAGG ACTTGTTATT GATAAAAATA TCAATATGGG GCATTTAAAA TATGTTATCA TAACATTTAT AAGAAGCTTT TTTGAAAATT CTAATATTGA ACTACGTTTT AGACCTAGCT TTTTCCCATT TACAGAGCCT TCTGCCGAGG TTGATATTCG CATGAATAAA AATGATAAAT GGCTTGAGGT CCTTGGGTGT GGGATGGTAC ATCCAAATGT GCTTAAAAAT ATTGATATTA ACAGAAGTGA GTATCAAGGT TTTGCGTTTG GCCTTGGAGT AGAACGCTTT GCAATGCTAA AATATAATAT CAAAGATTTA AGACGATTTT TTGAAGGAGA TATACGTTGG CTTAAACATT ATAATTTTGA GAGTTTTGAT ATACCGAATT TAGCAGGAGG TTTGACAAAA

Details for RiprA.00163.a.B1.GE27186

HARVESTED ON:	8/17/2009
SEQUENCED ON:	9/29/2009
EXPECTED MW:	41kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK NIETILKLAE EKIVLVHNLK DLQEYKVEFL GRNGIVTSEL KKLGSLINGQ KRKEFGLKIN TLKDKIHDII KAKAQNLEAE ELHLKLAADK IDLTIPARRY KQGSIHPITQ CMDELIQVFS QFGFTIENGP NIENDFYNFT ALNFEYDHPA RQMHDTFYLK GHENDKPLLL RTHTSTVQIR AMKNGKPPFR FIAPGRTYRS DSDMTHTPMF HQIEGLVIDK NINMGHLKYV IITFIRSFFE NSNIELRFRP SFFPFTEPSA EVDIRMNKND KWLEVLGCGM VHPNVLKNID INRSEYQGFA FGLGVERFAM LKYNIKDLRR FFEGDIRWLK HYNFESFDIP NLAGGLTK

Validated NT Sequence

anggagnnat accatggctc accaccacca ccaccatatg aaaaatatag aaacaatatt aaaacttgct gaggaaaaaa ttgtattagt acataactta aaagatttgc aagaatataa agtagaattt ttaggtagaa acggtatagt aacgagtgag cttaaaaaat taggtagttt aataaatgga caaaaacgta aagaattcgg cttaaaaatc aacacattaa aagataaaat acacgatata ataaaagcaa aagcgcaaaa tttagaggca gaagaactgc acttaaaact tgctgccgat aaaattgatt taaccattcc tgcaagaaga tataaacaag gttctattca tccaataact caatgtatgg atgagttaat acaagtattt tcgcagtttg gttttactat agaaaatgga ccgaatatag aaaatgattt ttataatttt actgctctca attttgaata tgaccatcca gcaaggcaga tgcacgatac tttttattta aaaggtcatg aaaatgataa accattgcta ttacgtactc acacctcaac agttcagatt agagcaatga aaaacggcaa accgcctttt agatttatag cacctggtcg gacttataga tcagattcag atatgacaca tactccaatg ttccaccaaa tagaaggact tgttattgat aaaaatatca atatggggca tttaaaatat gttatcataa catttataag aagctttttt gaaaattcta atattgaact acgttttaga cctagctttt tcccatttac agagccttct gccgaggttg atattcgcat gaataaaaat gataaatggc ttgaggtcct tgggtgtggg atggtacatc caaatgtgct taaaaatatt gatattaaca gaagtgagta tcaaggtttt gcgtttggcc ttggagtaga acgctttgca atgctaaaat ataatatcaa agatttaaga cgattttttg aaggagatat acgttggctt aaacattata attttgagag ttttgatata ccgaatttag caggaggttt gacaaaataa gtgagtaaga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa atatagaaac aatattaaaa cttgctgagg aaaaaattgt attagtacat aacttaaaag atttgcaaga atataaagta gaatttttag gtagaaacgg tatagtaacg agtgagctta aaaaattagg tagtttaata aatggacaaa aacgtaaaga attcggctta aaaatcaaca cattaaaaga taaaatacac gatataataa aagcaaaagc gcaaaattta gaggcagaag aactgcactt aaaacttgct gccgataaaa ttgatttaac cattcctgca agaagatata aacaaggttc tattcatcca ataactcaat gtatggatga gttaatacaa gtattttcgc agtttggttt tactatagaa aatggaccga atatagaaaa tgatttttat aattttactg ctctcaattt tgaatatgac catccagcaa ggcagatgca cgatactttt tatttaaaag gtcatgaaaa tgataaacca ttgctattac gtactcacac ctcaacagtt cagattagag caatgaaaaa cggcaaaccg ccttttagat ttatagcacc tggtcggact tatagatcag attcagatat gacacatact ccaatgttcc accaaataga aggacttgtt attgataaaa atatcaatat ggggcattta aaatatgtta tcataacatt tataagaagc ttttttgaaa attctaatat tgaactacgt tttagaccta gctttttccc atttacagag ccttctgccg aggttgatat tcgcatgaat aaaaatgata aatggcttga ggtccttggg tgtgggatgg tacatccaaa tgtgcttaaa aatattgata ttaacagaag tgagtatcaa ggttttgcgt ttggccttgg agtagaacgc tttgcaatgc taaaatataa tatcaaagat ttaagacgat tttttgaagg agatatacgt tggcttaaac attataattt tgagagtttt gatataccga atttagcagg aggtttgaca aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat